scholarly journals Features of free oxidation in liver homogenates of rats in combined action of copper and subtoxic level of stearate potassium and sodium

2016 ◽  
Author(s):  
O. V. Lototska
Keyword(s):  
Free Radical ◽  
Liver Homogenate ◽  
Sodium Stearate ◽  
Free Radical Oxidation ◽  
The Body ◽  
Antioxidant Systems ◽  
Radical Oxidation ◽  
Combined Action ◽  
Liver Homogenates ◽  
Free Oxidation

Toxic effects of heavy metals on animals and humans is aggravating the presence of surfactants. The aim of our research was to identify the peculiarities of free radical oxidation and antioxidant protection in the use of drinking water with subtoxic doses of potassium and sodium stearates in combination with copper on the body of warmblooded animals, such as the liver of experimental rats. Analysis of indicators allows asserting that in experimental rats liver homogenate observed activation of free radical oxidation, the intensity of which depended on the concentration of stearates in water. More pronounced changes were in animals that consumed water with potassium stearate.The action of potassium stearate observed inhibition of antioxidant systems while under the influence of sodium stearate – its activation. Changes in performance were more pronounced in combination stearates with copper.

Gender differences in biochemical measurement reflecting the state of free-radical oxidation and antioxidant protection among workers in metallurgical production

2019 ◽  
Vol 1 (10) ◽  
pp. 877-881
Author(s):  
I. A. Umnyagina ◽  
L. A. Strakhova ◽  
T. V. Blinova
Keyword(s):  
Oxidative Stress ◽  
Gender Differences ◽  
Free Radical ◽  
Biochemical Parameters ◽  
Free Radical Oxidation ◽  
The Body ◽  
Antioxidant Protection ◽  
Radical Oxidation ◽  
Metallurgical Production ◽  
Production Factors

Introduction. To date, age and sex differences have been established for many biochemical parameters. Gender differences in indicators for systems such as antioxidant, thiol-disulfide, oxidative stress and inflammation systems are absent or under study.The aim of the study was to identify gender differences in biochemical parameters reflecting the functioning of antioxidant systems of the body and free radical oxidation in workers of metallurgical production, in contact with harmful production factors.Materials and methods. The blood of men and women working at the metallurgical enterprise of the Nizhny Novgorod region (n=80) under the influence of a complex of physical and chemical production factors was studied. Total oxidative stress, total antioxidant capacity of serum, glutathione levels were studied by photometric biochemical methods. Levels of C-reactive protein and 8-hydroxy–2-deoxyguanosine were studied by ELISA.Results. The average amount of peroxides in the serum of women exceeded 1.6 times this figure in men. In the group of men, the content of 8-Ondg was higher by 26% (p=0.012), the level of GS-by 12% (p=0.019), the activity of SOD — by 1.5–2 times (p=0.0001), the level of CRP — by 2 times (p=0.008) compared to similar indicators in women.Conclusions. Studies of gender differences in workers under the influence of harmful production factors will allow more effective approach to the etiology, treatment and prognosis of production-related diseases. Indicators of oxidative stress and antioxidant protection can be indicators of the health of workers under the influence of harmful industrial factors and be important in the prevention of diseases associated with oxidative stress.

scholarly journals ACETAMINOPHEN EFFECT ON FREE RADICAL OXIDATION INDICES IN RATS WITH TYPE 2 DIABETES MELLITUS

2017 ◽  
Author(s):  
O. B. Furka ◽  
I. B. Ivanusa ◽  
M. M. Mykhalkiv ◽  
I. M. Klishch
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Free Radical ◽  
Liver Homogenate ◽  
Free Radical Oxidation ◽  
Maximum Increase ◽  
Radical Oxidation ◽  
Age Related

Background. Acetaminophen is a drug used to relieve pain syndrome. It is used both independently and in composition of combined drugs. Type 2 diabetes is an age-related disease that is associated with a violation of insulin synthesis by pancreas.Objective. The aim of the research was to study the effect of acetaminophen on major free radical oxidation indices of rats with type 2 diabetes mellitus in time dynamics.Methods. We conducted two series of experiments. The first series comprised rats with type 2 diabetes mellitus and acute acetaminophen toxic lesions. The second series involved rats with type 2 diabetes mellitus and acetaminophen administration at a dose of 55 mg/kg for the period of 7 days.Results. Administration of acetaminophen for rats with type 2 diabetes mellitus caused the increase in the content of malondialdehyde, diene and triene conjugates and Schiff bases in blood plasma and malondialdehyde, diene and triene conjugates in liver homogenate. The maximum increase in these indices was observed on the first day of the experiment. Gradually these indices decreased on the 3rd, 5th and 7th days of the experiment.Conclusions. Free radical oxidation increased in both series of the experiment. This process developed in rats with type 2 diabetes mellitus and acute acetaminophen toxic lesions more intensively, than in rats with type 2 diabetes mellitus and administration of acetaminophen at the highest therapeutic dose during 7 days.

scholarly journals Specific features of biological oxidation processes in helminthiases of horses

2020 ◽  
Vol 61 (3) ◽  
pp. 126-130
Author(s):  
Larisa V. Pilip ◽  
◽  
Olga V. Byakova ◽  
Maria E. Kazakova ◽  
◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
The Body ◽  
Luminous Intensity ◽  
Radical Oxidation ◽  
Pathological Conditions ◽  
Oxidized Products

The chemical processes occurring in the animal body under pathological conditions are of interest to researchers. The objective of the research was to study the indicators of lipid peroxidation and antioxidant activity in horses infested with helminthiases and treated with anthelmintic paste with an antioxidant. The study has proved the role of parasitic infestation in accelerating the processes of free radical oxidation, which is manifested by a decrease in the light sum of radicals (36.62-39.21), the maximum luminous intensity (6.13-6.98) and antioxidant activity (5.65-6.2) at the start of the research. Chronic invasion increases the concentration of under-oxidized products up to 38.59, and the rate of initiating free radical oxidation – up to 6.95, the values of antioxidant activity being stably low (5.51-5.91). An important role in the regulation of lipid peroxidation in horses during helminthiases, as well as in the process of deworming, is played by the antioxidants contained in anthelmintic preparations. The antioxidant santohin included as a compound in the «Alezan» preparation suppresses enhanced peroxidation and activates the antioxidant protection of the body, which is reflected in a decrease in the light sum of the radicals to as low as 26.73, a maximum luminous intensity to – 3.76, and an increase in antioxidant activity – up to 7.16.

scholarly journals Assessment of the body’s nonspecific defense in workers exposed to noxious factors of chemical and petrochemical industry

2016 ◽  
Vol 97 (5) ◽  
pp. 784-792 ◽  
Author(s):  
R I Sabitova ◽  
E D Kravets ◽  
E F Galiullina ◽  
D F Shakirov ◽  
F Kh Kamilov ◽  
...  
Keyword(s):  
Free Radical ◽  
Energy Metabolism ◽  
Petrochemical Industry ◽  
Antioxidant Defense ◽  
Free Radical Oxidation ◽  
The Body ◽  
Experimental Plant ◽  
Electrolyte Metabolism ◽  
Radical Oxidation ◽  
Oxidation Processes

Aim. To study the processes of free-radical oxidation, antioxidant defense, energy metabolism, electrolyte metabolism, and cytokine system reflecting the state of the non-specific defense of the body in workers exposed to complex of toxic substances under the conditions of manufacturing, substantiating the importance of indicators of preclinical diagnosis of disease development risk.Methods. The study included 90 workers of JSC «Experimental Plant Neftekhim», 95 workers of JSC «Kaustic» and 101 workers of JSC «Ufa plant of elastomeric materials, products and structures». Materials for the study were blood, mixed saliva, gingival fluid and urine. The laboratory studies were performed to evaluate the free radical oxidation processes, antioxidant defense, energy metabolism, electrolyte metabolism, and cytokine system. Taking into account the features of industrial factors, particularly their effects on the workers bodies, routes of toxic compounds entry by inhalation, oral cavity and skin of the hands, three professional groups were formed (A, B, C). Group A included employees having constant contact with chlororganic compounds. The group B included persons who have constant contact with the higher and lower aromatic hydrocarbons. A group C consisted of employees who have constant contact with a mixture of chemicals: rubber compound containing carcinogens - benzo(a)pyrene, NDMA, nitrosodiethylamine; white carbon black, rubber dust, talc, amine compounds, sulfur dioxide, carbon monoxide. The control group consisted of employees of administrative and managerial staff.Results. The study revealed that one of the major pathogenetic mechanisms of action of chemical and petrochemical industry hazards is activation of free radical oxidation.Conclusion. Among the mechanisms of the influence of chemical contaminants of working environment a leading role play intensification of free radical oxidation processes, failure and/or inhibition of the antioxidant defense components.

scholarly journals S-ADEMETHIONINE EFFECT ON THE ACTIVITY OF FREE RADICAL OXIDATION PROCESSES AND ARGININE/CITRULLINE CYCLE WITH DOXORUBICIN-INDUCED LIVER INJURY

2020 ◽  
Vol 20 (2) ◽  
pp. 156-161
Author(s):  
G.S. Maslova ◽  
R.I. Skrypnyk ◽  
І.М. Skrypnyk
Keyword(s):  
Free Radical ◽  
Sodium Chloride ◽  
Liver Injury ◽  
Liver Homogenate ◽  
Free Radical Oxidation ◽  
Thiobarbituric Acid ◽  
Arginase Activity ◽  
Radical Oxidation ◽  
Group I ◽  
Doxorubicin Administration

Seeking for effective methods for doxorubicin-induced toxicity prevention is of great clinical importance. The aim of the study is to investigate the effect of S-ademethionine on the activity of free radical oxidation and antioxidant protection and arginine / citrulline cycle, and the relationship between the indicators during the modeled doxorubicin-induced liver injury in rats. Materials and methods. The series of study were performed on 30 white non-linear adult rats, 15 (50%) males, 15 (50%) females, weighing 160-220 g. The experimental animals were divided into 3 groups: I group (n=10) included rats (5 males and 5 females), which during 3 days were intraperitoneally administered with doxorubicin of 5 mg/kg/day to achieve cumulative dose of 15 mg/kg and 0.9% sodium chloride solution 1 ml; ІІ group (n=10) included rats (5 males and 5 females), which for 3 days were administered with doxorubicin in dosage similar to that in group I and S-ademethionine intraperitoneally in a dose of 100 mg/kg/day with a total dose of 300 mg/kg; ІІІ group (n=10) included rats (5 males and 5 females), which were injected intraperitoneally for 3 days with 0.9% sodium chloride 1 ml solution by 2 injections. Thiobarbituric acid reactants, arginine, catalase, arginase and ornithine decarboxylase activity were studied in the liver homogenate. Results. The rats of group I in the course of doxorubicin administration demonstrated the growth of the thiobarbituric acid reactants in 2.57 times (p=0.002) in the liver homogenate that was accompanied by 1.7-fold decreased arginase activity compared to the control group III. An inverse correlation was found between the content of thiobarbituric acid reactants and arginase activity in the liver homogenate of group I rats (r=-0.71; p=0.02). Coadministration of S-ademethionine and doxorubicin administration led to 1.4-fold increase in catalase activity in the liver homogenate of rats compared to the control (p=0.009) that reduced the severity of oxidative stress and prevented changes in the arginine/citrulline cycle. Conclusions: S-ademethionine combined with doxorubicin, reduces the activity of free radical oxidation by potentiating the production of antioxidant protective enzymes that prevent impairments in the arginine / citrulline cycle.

scholarly journals Correction of free radical processes and Mitochondrial dysfunction in rats poisoned by sodium nitrite and tobacco smoke, mildronate preparation

2017 ◽  
Author(s):  
P. H. Lykhatskyi
Keyword(s):  
Free Radical ◽  
Sodium Nitrite ◽  
Tobacco Smoke ◽  
Age Groups ◽  
Free Radical Oxidation ◽  
Premature Death ◽  
The Body ◽  
Mitochondrial Enzymes ◽  
Radical Oxidation ◽  
Different Ages

Introduction. According to the WHO, smoking takes the second place in the list of reasons that cause premature death of people. The interest in clarifying the mechanisms of action on the organism of nitrites and nitrates and the links of the pathogenesis of gemic hypoxia, which thus arises, is due to their widespread use in industry, agriculture and medicine. Therefore, the study of the combined effects of several xenobiotics on the body is feasible and relevant.The aimof the study –to investigate the effectiveness of using antihydroxant Mildronate and its influence on the oxidative processes in the body of rats of different ages infected with sodium nitrite on the background of 45 days of tobacco intoxication.Research methods. Under the conditions of damage to rats of different ages, sodium nitrite on the background of tobacco intoxication was determined in the blood of the content of methemoglobin (MetHb) in reaction with acetone cyanadirin and carboxyhemoglobin (HbCO) in reaction with hexacyanone (III) potassium. The activity of lipoperoxidation processes was assessed by the content of TBА – active products (TBА – AP) in the serum of blood, lungs and myocardium of experimental animals. The evaluation of the functioning of the bioenergetic processes was carried out using the activity of succinate dehydrogenase (SAD), which was studied by the reaction of reduction of potassium ferricyanide, which solution has a yellow color, to colorless ferrocyanide of potassium succinate by the action of EDA and cytochrome oxidase (CO) by the oxidation reaction of dimethyl-n-phenylenediamine.Results and Discussion. It has been established that the defeat of rats of different age groups by sodium nitrite (24 and 72 h before the end of the experiment) on the background of 45 days intoxication with tobacco smoke leads to the activation of free radical oxidation processes, indicating an increase in the content of methemoglobin in the blood of affected animals and TBА-AP in serum of blood, lungs and myocardium after poisoning. At the same time in the blood the content of carboxyhemoglobin increases. In the body, there is a mixed hypoxia (hemitic and circulatory), which leads to disturbances in the functioning of the mitochondrial oxidation chain. The most susceptible to the activity of toxicotoxins were immature animals, and in older animals there was a significant change in the activity of mitochondrial enzymes in the myocardium after the injury, which was more pronounced in comparison with other age groups of rats. In order to correct the revealed violations, a drug of metabolic action with antihypoxant properties of mildronate was used, which positively influenced the activity of lipoperoxidation processes and the activity of mitochondrial enzymes under hypoxia conditions.Conclusions. The obtained results indicate the expediency of inclusion of mildronate in the complex treatment of poisonings of different genesis.

scholarly journals Indices of free-radical oxidation and antioxidant system of rats with acute hepatitis combined with merkazolil-induced hypothyroidism

2015 ◽  
Vol 17 (3) ◽  
Author(s):  
O. Y. Zarichna
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Acute Hepatitis ◽  
Liver Homogenate ◽  
Free Radical Oxidation ◽  
Protection System ◽  
Antioxidant Protection ◽  
Radical Oxidation ◽  
Galactosamine Hepatitis ◽  
Antioxidant Protection System

<p>In experiments on rats with modulated hypothyroidism, acute galactosamine hepatitis, acute hepatitis combined<br />with merkazolil-induced hypothyroidism the changes in the indices of free-radical oxidation in blood and liver<br />homogenate were studied. Results of the investigation indicate a pronounced increase of lipid peroxidation products,<br />both in blood and in liver homogenate against the backdrop of exhaustion of the enzymatic and non-enzymatic links<br />antioxidant protection system in animals with hypothyroidism. In case of acute galactosamine hepatitis increase of<br />lipid peroxidation products, both in blood and in liver homogenate against the background of activation units both<br />components of the antioxidant protection system was established. The most significant inhibition of the enzymatic<br />and non-enzymatic links antioxidant protection system suffered rats with acute hepatitis on the background of<br />hypothyroidism. It is concluded that failure revealed a mutual gain reflects the negative impact of acute toxic liver<br />injury and thyroid hormone deficiency.</p>

scholarly journals Correction of the state of antioxidant protection system of the fowl’s organism under the circumstances of being poisoned by Derosal

2020 ◽  
Vol 22 (98) ◽  
pp. 46-50
Author(s):  
O. S. Kochevenko ◽  
I. O. Zhukova ◽  
O. M. Bobrytska ◽  
I. O. Kostiuk ◽  
L. A. Vodopyanova
Keyword(s):  
Superoxide Dismutase ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
The State ◽  
The Body ◽  
Superoxide Dismutase Activity ◽  
Antioxidant Protection ◽  
Diene Conjugates ◽  
Active Forms Of Oxygen ◽  
Radical Oxidation

In fact, Free radical oxidation is one of the required factors of homeostasis as far as the active forms of oxygen, which have unpaired electron, perform useful functions, they are necessary intermediaries of numerous processes of normal cells’ functioning such as biosynthesis of prostaglandins and leukotriene as well as other biologically active substances. Also, they take part in pathological processes such as: inflammation, fever and others. Free radical oxidation is the process of direct oxygen transferring to the substrate forming peroxide, ketones, aldehydes, etc. Some amino acids, proteins, water and carbohydrates prone to the processes of peroxidation, but crucial place is granted to lipids at the expanse of fatty acids which are included to their composition. The article highlights the information concerning the analysis of the state of antioxidant system of hens in the process of feeding them with subtoxic doses of fungicide of Derosal (BAYER, Germany) (0.1 LD50 for a fowl, 900 mg/kg of the body mass) which active ingredient is benzimidazole carbamate (carbendazim, BMC). It presents the changes of these indicators in the process of using pharmacological and natural antioxidants. The analysis has figured out that giving Derosal to the hens without corrections (II group) has been followed with the activation of the process of lipids’ peroxidation (POL) in the blood plasma, that is followed with the increase of concentration of primary and secondary lipoperoxidation products – diene conjugates (DC) and TBA-active products on the average up to 21.0–25.0 %, catalase activity – up to 38.5 % and decrease of superoxide dismutase activity (SOD) up to 21.8 % and АОА – up to 17.8 %, that shows the adverse effects of pesticides on the system of antioxidant protection of the organism. In the fowls’ groups III, IV and V the adding of antioxidant E-selenium, ascorbic acid and thermally untreated buckwheat seeds as the source of  quercetins and anthocyanins has led to the induction of antioxidant resources that is shown by the decrease in comparison with the control of content of diene conjugates, malonic dialdehyde and catalase (in average up to 13.6–33.6–18.0 % accordingly) and compensatory strengthening of superoxide dismutase activity and general activity of antioxidant (АОА) on the average up to 44.9–72.1–76.5 % and 15.3–13.4–21.7 % accordingly. In comparison with group II the activity of catalase has been lower as well as the activity of superoxide dismutase has been higher in all the groups almost in 1.5–2 times (Р ≤ 0.001).

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN BUAS-BUAS (Premna serratifolia L.) ASAL KABUPATEN MELAWI PROVINSI KALIMANTAN BARAT DENGAN METODE DPPH

2020 ◽  
Vol 3 (2) ◽  
pp. 405-412
Author(s):  
Weni Puspita ◽  
◽  
Dina Yuspita Sari ◽  
Ika Ristia Rahman ◽  
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Free Radical ◽  
Cell Damage ◽  
Antioxidant Properties ◽  
Free Radical Oxidation ◽  
The Body ◽  
Radical Oxidation ◽  
Ic50 Values ◽  
Ethanol Extracts

Buas (Premna serratifolia L.) contains secondary metabolites of flavonoids, saponins, tannins and triterpenoids/steroids, where flavonoids are known to have antioxidant properties and counteract free radicals. Antioxidants are compounds that can fight toxic hazards and reduce cell damage to the body caused by free radical oxidation. The purpose of this study was to determine the antioxidant activity of ethanol extracts of buas-buas leaves was carried out by DPPH (2,2-diphenyl-1-picrylhydrazyl), using UV-Vis spectrophotometry at wavelength 518.40 nm with various concentrations of 10 ppm, 20 ppm, 30 ppm, 40 ppm and 50 ppm. The results showed that Buas Buas leaves (Premna serratifolia L.) had an antioxidant activity with IC50 values ??of 20.66 ug / mL.

OXIDATIVE STRESS IN EXPERIMENTAL PERIODONTITIS

2020 ◽  
pp. 67-72
Author(s):  
Zakhvatov A.N. ◽  
Hajdar D.A. ◽  
Tarasova T.V. ◽  
Avanesov A.M. ◽  
Samarina D.V. ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
Total Activity ◽  
The Body ◽  
Antioxidant Potential ◽  
Radical Oxidation ◽  
Periodontal Tissues ◽  
Markers Of Oxidative Stress ◽  
Experimental Periodontitis

Relevance. The study of bioradical processes in periodontitis in the dynamics of the disease and the establishment of the role of predictors of periodontal tissue damage will provide a deeper understanding of the links of pathogenesis and, on this basis, introduce new therapeutic and diagnostic technologies into practice. The purpose of the study: Assessment of the level of markers of oxidative stress in experimental periodontitis in the dynamics of the study. Materials and methods. The study was conducted on white non-linear rats, reproducing the model of periodontitis proposed by Shkolnaya K. D., Atrushkevich V. G. (Patent RU No. 2625295 of 12.07.2017). The total activity of free radical oxidation and the body's antioxidant potential were evaluated by biochemiluminescence, the intensity of lipoperoxidation processes, and the enzyme antioxidant potential. Results. A comprehensive analysis of the overall oxidative and antioxidant capacity of the body was carried out, during which it was revealed that progressive experimental periodontitis causes inhibition of the antioxidant potential and is characterized by a violation of the processes of neutralization of reactive oxygen species and the inability to inhibit the launched reactions of free radical oxidation, as evidenced by the dynamics of an increased level of markers of oxidative stress. Conclusion. Thus, in periodontitis, oxidative stress develops, which contributes to the progression of destructive processes of periodontal tissues, which substantiates the feasibility of using markers of the intensity of free radical oxidation as diagnostic and prognostic criteria for the course of the disease and the introduction of antioxidant-type drugs in complex therapy.

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