Inhibitor of Differentiation-3 Mediates High Fat Diet-Induced Visceral Fat Expansion

Background. The condensed fermentative extract of bonito (BoE), skipjack tuna (Katsuwonus pelamis), has claimed its health conditioning effects against lifestyle-related diseases such as hypertension and type 2 diabetes. Methods. We evaluated the antiobesity and anti-inflammatory effects of BoE on mice fed a high-fat diet (HFD). Mice (9 weeks of age) were maintained for 11 weeks on HFD with or without BoE (50 mg or 500 mg/kg). Results. Compared with untreated mice, BoE50 or BoE500 mice achieved maximum weight reductions of 7.4% (males) and 11.4% (females), and visceral fat in male BoE500 mice was more decreased among all mice (P = 0.00459). Furthermore, an antiobesity gene uncoupling protein-1 was significantly induced in the visceral fat tissues of male BoE500 (P = 0.0110) and female BoE50 and BoE500 mice (P = 0.0110 and P = 0.0110, resp.). Finally, we detected reduced amount of granulocyte-colony stimulating factor (P = 0.0250) in the sera of female BoE50 and interleukin- (IL-) 5 (P = 0.0120), IL-6 (P = 0.0118), and IL-13 (P = 0.0243) in female BoE500 mice. Conclusion. The antiobesity and anti-inflammatory effects of BoE were demonstrated with our examination system and any toxic adverse effects were not observed in mice during the 3-month investigation.

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Cinnamaldehyde Inhibits Visceral Fat Accumulation and the levels of Glucose, Insulin, Leptin in Serum of rats fed with High Fat Diet (HFD)

Research Journal of Pharmacy and Technology ◽

10.5958/0974-360x.2015.00306.6 ◽

2015 ◽

Vol 8 (12) ◽

pp. 1701

Author(s):

Haripriya Doraiswamy ◽

Vijayalakshmi Kirshnamurthy

Keyword(s):

Visceral Fat ◽

High Fat Diet ◽

High Fat ◽

Fat Accumulation ◽

Visceral Fat Accumulation

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Dietary nitrite reverses features of postmenopausal metabolic syndrome induced by high-fat diet and ovariectomy in mice

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.00360.2016 ◽

2017 ◽

Vol 312 (4) ◽

pp. E300-E308 ◽

Cited By ~ 14

Author(s):

Kazuo Ohtake ◽

Nobuyuki Ehara ◽

Hiroshige Chiba ◽

Genya Nakano ◽

Kunihiro Sonoda ◽

...

Keyword(s):

Nitric Oxide ◽

Insulin Resistance ◽

Metabolic Syndrome ◽

Visceral Fat ◽

High Fat Diet ◽

Hormone Replacement ◽

Dependent Manner ◽

High Fat ◽

Alternative Source ◽

Enos Activity

Menopausal women are at greater risk of developing metabolic syndrome with reduced endothelial nitric oxide synthase (eNOS) activity. Hormone replacement therapy increases eNOS activity and normalizes some characteristics of metabolic syndrome. We hypothesized that nitric oxide (NO) supplementation should have a therapeutic effect on this syndrome. We examined the effect of dietary nitrite in a mouse model with postmenopausal metabolic syndrome induced by ovariectomy (OVX) and a high fat diet (HF). C57BL/6 female mice were divided into five groups, sham+normal fat diet (NF), sham+ HF, OVX+HF with or without sodium nitrite (50 mg and 150 mg/l) in the drinking water. Daily food intake and weekly body weight were monitored for 18 wk. OVX and HF significantly reduced plasma levels of nitrate/nitrite (NOx), and mice developed obesity with visceral hypertrophic adipocytes and increased transcriptional levels of monocyte chemoattractant protein-1, TNF-α, and IL-6 in visceral fat tissues. The proinflammatory state in the adipocytes provoked severe hepatosteatosis and insulin resistance in OVX+HF group compared with sham+NF group. However, dietary nitrite significantly suppressed adipocyte hypertrophy and transcriptions of proinflammatory cytokines in visceral fat in a dose-dependent manner. The improvement of visceral inflammatory state consequently reversed the hepatosteatosis and insulin resistance observed in OVX+HF mice. These results suggest that an endogenous NO defect might underlie postmenopausal metabolic syndrome and that dietary nitrite provides an alternative source of NO, subsequently compensating for metabolic impairments of this syndrome.

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Visceral Fat Accumulation Induced by a High-fat Diet Causes the Atrophy of Mesenteric Lymph Nodes in Obese Mice

Obesity ◽

10.1038/oby.2008.55 ◽

2008 ◽

Vol 16 (6) ◽

pp. 1261-1269 ◽

Cited By ~ 40

Author(s):

C.S. Kim ◽

S.C. Lee ◽

Y.M. Kim ◽

B.S. Kim ◽

H.S. Choi ◽

...

Keyword(s):

Lymph Nodes ◽

Visceral Fat ◽

High Fat Diet ◽

Obese Mice ◽

High Fat ◽

Mesenteric Lymph Nodes ◽

Fat Accumulation ◽

Visceral Fat Accumulation ◽

Mesenteric Lymph

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Effect of High Fat Diet on Weight Loss Through the Expression of Uncouple Protein 1 in Mice Visceral Fat

Folia Medica Indonesiana ◽

10.20473/fmi.v56i3.22190 ◽

2020 ◽

Vol 56 (3) ◽

pp. 223

Author(s):

Indira Syahraya ◽

Hermina Novida ◽

Lilik Herawati ◽

Purwo Sri Rejeki

Keyword(s):

Weight Loss ◽

Visceral Fat ◽

High Fat Diet ◽

Significant Weight Loss ◽

Uncouple Protein ◽

High Fat ◽

Immunohistochemistry Staining ◽

Key Factor ◽

Weight Loss Group ◽

Ucp1 Expression

Obesity has become an epidemic around the world. High fat diet (HFD) have been implemented as one of intervention to battle obesity. Uncouple protein 1 (UCP1) is one of the key factor on energy expenditure. The aim of this experiment is to see the macronutrients composition on weight loss and UCP1 expression in the visceral fat. Fifty male mice, 2-3 months old, 18-30 grams, were put in five different groups. K1 (20% protein, 62.0% carbohydrate, 12% fat), K2 (60% protein, 0% carbohydrate, 30% fat), K3 (45% protein, 0% carbohydrate, 45% fat), K4 (30% protein, 0% carbohydrate, 60% fat), K5 (15% protein, 0% carbohydrate, 75% fat). The experiment was done in four weeks, mice body weight was measured every week. UCP1 expression seen using immunohistochemistry staining was measured at the end of the fourth week. Significant weight loss was achieved by K4 (-9.60±3.81) gram by the end of week four (p<0.05). K4 had the least amount of visceral fat. The result was that K4 achieved a significant visceral fat mass (0.02±0.06) gram compared to K1 (0.53±0.08) gram. Compared to other groups K5 expressed UCP1 more than the others (3.78±3.72) cphfp. HFD fed groups produced significant weight loss, group that had the greatest weight loss is K4. Meanwhile, each group had a variety of UCP1 expression.

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Effects of Collagen Peptide Administration on Visceral Fat Content in High-Fat Diet-Induced Obese Mice

Journal of Nutritional Science and Vitaminology ◽

10.3177/jnsv.67.57 ◽

2021 ◽

Vol 67 (1) ◽

pp. 57-62

Author(s):

Ran WATANABE ◽

Mana YAMAGUCHI ◽

Kyosuke WATANABE ◽

Muneshige SHIMIZU ◽

TAKAHASHI Azusa ◽

...

Keyword(s):

Visceral Fat ◽

High Fat Diet ◽

Fat Content ◽

Obese Mice ◽

High Fat ◽

Collagen Peptide ◽

Visceral Fat Content

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Erchen Decoction Mitigates Lipid Metabolism Disorder by the Regulation of PPARγ and LPL Gene in a High-Fat Diet C57BL/6 Mice Model

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2020/9102475 ◽

2020 ◽

Vol 2020 ◽

pp. 1-8

Author(s):

Mengting Zhang ◽

Yanfei Shao ◽

Bizhen Gao ◽

Jicheng Chen ◽

Ping Zhang ◽

...

Keyword(s):

Skeletal Muscle ◽

Body Weight ◽

Lipid Metabolism ◽

Visceral Fat ◽

Protein Level ◽

High Fat Diet ◽

Blood Lipid ◽

Abdominal Circumference ◽

High Fat ◽

Gene And Protein Expression

Erchen decoction (ECD) is a common treatment prescribed in traditional Chinese medicine (TCM) clinics, which has remarkable efficacy in the treatment of obesity, fatty liver, hyperlipidemia, diabetes, and other diseases caused by phlegm. In this study, we investigated the effect that ECD had on the lipid metabolism induced by high-fat diet in C57BL/6 mice. Body weight, body length, and abdominal circumference were detected. Blood lipid content was measured via biochemical assay kit. The gene and protein expression of PPARγ and LPL in visceral fat and skeletal muscle of mice was measured by real-time PCR and western blot. The research discovered that the phlegm-resolving effect that ECD had on high-fat diet mice was mainly manifested as reduced body weight, Lee’s index, abdominal circumference, and level of TG and TC. Meanwhile, we observed significantly increased PPARγ mRNA and protein level in visceral fat and PPARγ and LPL protein level in skeletal muscle in the ECD group. Contrarily, a decrease in PPARγ mRNA level in skeletal muscle in the ECD group was observed. Therefore, we speculate that ECD regulates the lipid metabolic disorder by decreasing the blood lipid level. Moreover, the potential molecular mechanism of ECD is to promote the expression of PPARγ in visceral fat and skeletal muscle and the expression of LPL in skeletal muscle.

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