Application of Antimicrobial Peptides against Microcystis aeruginosa to Control Harmful Algal Blooms

2018 ◽  
Vol 36 (4) ◽  
pp. 601-609 ◽  
Author(s):  
Sang-Il Han ◽  
Yoonkyung Park ◽  
Yoon-E Choi
Keyword(s):  
Antimicrobial Peptides ◽  
Microcystis Aeruginosa ◽  
Harmful Algal Blooms ◽  
Algal Blooms

scholarly journals Transcriptional Analysis of Microcystis aeruginosa Co-Cultured with Algicidal Bacteria Brevibacillus laterosporus

2021 ◽  
Vol 18 (16) ◽  
pp. 8615
Author(s):  
Yulei Zhang ◽  
Dong Chen ◽  
Ning Zhang ◽  
Feng Li ◽  
Xiaoxia Luo ◽  
...  
Keyword(s):  
Microcystis Aeruginosa ◽  
Harmful Algal Blooms ◽  
Algal Blooms ◽  
Control Method ◽  
Acid Synthesis ◽  
Transcriptional Analysis ◽  
Economic Losses ◽  
Algicidal Bacteria ◽  
Brevibacillus Laterosporus ◽  
Expression Of Genes

Harmful algal blooms caused huge ecological damage and economic losses around the world. Controlling algal blooms by algicidal bacteria is expected to be an effective biological control method. The current study investigated the molecular mechanism of harmful cyanobacteria disrupted by algicidal bacteria. Microcystis aeruginosa was co-cultured with Brevibacillus laterosporus Bl-zj, and RNA-seq based transcriptomic analysis was performed compared to M. aeruginosa, which was cultivated separately. A total of 1706 differentially expressed genes were identified, which were mainly involved in carbohydrate metabolism, energy metabolism and amino acid metabolism. In the co-cultured group, the expression of genes mainly enriched in photosynthesis and oxidative phosphorylation were significantly inhibited. However, the expression of the genes related to fatty acid synthesis increased. In addition, the expression of the antioxidant enzymes, such as 2-Cys peroxiredoxin, was increased. These results suggested that B. laterosporus could block the electron transport by attacking the PSI system and complex I of M. aeruginosa, affecting the energy acquisition and causing oxidative damage. This further led to the lipid peroxidation of the microalgal cell membrane, resulting in algal death. The transcriptional analysis of algicidal bacteria in the interaction process can be combined to explain the algicidal mechanism in the future.

Quantifying the efficacy of diquat dibromide in controlling Microcystis aeruginosa and Aphanizomenon flos-aquae in comparison to copper sulfate and potassium permanganate

2019 ◽  
Vol 5 (1) ◽  
pp. 140-151 ◽  
Author(s):  
Sara Dia ◽  
Ibrahim Alameddine ◽  
Mutasem El-Fadel
Keyword(s):  
Potassium Permanganate ◽  
Microcystis Aeruginosa ◽  
Copper Sulfate ◽  
Harmful Algal Blooms ◽  
Algal Blooms ◽  
Freshwater Systems ◽  
Cyanobacterial Harmful Algal Blooms

Cyanobacterial harmful algal blooms (HABs) are an emerging problem worldwide, affecting many important freshwater systems.

scholarly journals Abundant Allelochemicals and the Inhibitory Mechanism of the Phenolic Acids in Water Dropwort for the Control of Microcystis aeruginosa Blooms

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2653
Author(s):  
Jixiang Liu ◽  
Yajun Chang ◽  
Linhe Sun ◽  
Fengfeng Du ◽  
Jian Cui ◽  
...  
Keyword(s):  
Microcystis Aeruginosa ◽  
Phenolic Acids ◽  
Harmful Algal Blooms ◽  
Algal Blooms ◽  
Inhibitory Effect ◽  
Simultaneous Action ◽  
Inhibitory Effects ◽  
Chl A ◽  
Ferulic Acids ◽  
Water Dropwort

In recent years, with the frequent global occurrence of harmful algal blooms, the use of plant allelopathy to control algal blooms has attracted special and wide attention. This study validates the possibility of turning water dropwort into a biological resource to inhibit the growth of harmful Microcystis aeruginosa blooms via allelopathy. The results revealed that there were 33 types of allelopathic compounds in the water dropwort culture water, of which 15 were phenolic acids. Regarding water dropwort itself, 18 phenolic acids were discovered in all the organs of water dropwort via a targeted metabolomics analysis; they were found to be mainly synthesized in the leaves and then transported to the roots and then ultimately released into culture water where they inhibited M. aeruginosa growth. Next, three types of phenolic acids synthesized in water dropwort, i.e., benzoic, salicylic, and ferulic acids, were selected to clarify their inhibitory effects on the growth of M. aeruginosa and their mechanism(s) of action. It was found that the inhibitory effect of phenolic acids on the growth of M. aeruginosa increased with the increase of the exposure concentration, although the algae cells were more sensitive to benzoic acid than to salicylic and ferulic acids. Further study indicated that the inhibitory effects of the three phenolic acids on the growth of M. aeruginosa were largely due to the simultaneous action of reducing the number of cells, damaging the integrity of the cell membrane, inhibiting chlorophyll a (Chl-a) synthesis, decreasing the values of F0 and Fv/Fm, and increasing the activity of the antioxidant enzymes (SOD, POD, and CAT) of M. aeruginosa. Thus, the results of this study indicate that both culture water including the rich allelochemicals in water dropwort and biological algae inhibitors made from water dropwort could be used to control the growth of noxious algae in the future.

scholarly journals Nutrient Loading and Viral Memory Drive Accumulation of Restriction Modification Systems in Bloom-Forming Cyanobacteria

mBio ◽  
2021 ◽  
Author(s):  
Spiridon E. Papoulis ◽  
Steven W. Wilhelm ◽  
David Talmy ◽  
Erik R. Zinser
Keyword(s):  
Water Quality ◽  
Microcystis Aeruginosa ◽  
Harmful Algal Blooms ◽  
Algal Blooms ◽  
Nutrient Loading ◽  
Major Contributor ◽  
Content Type ◽  
Global Threat ◽  
Restriction Modification ◽  
Restriction Modification Systems

Harmful algal blooms (HABs), caused by cyanobacteria like Microcystis aeruginosa , are a global threat to water quality and use across the planet. Researchers have agreed that nutrient loading is a major contributor to HAB persistence.

scholarly journals Isolation and Identification of Two Algae-Lysing Bacteria against Microcystis aeruginosa

Water ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 2485
Author(s):  
Jing Yang ◽  
Kai Qiao ◽  
Junping Lv ◽  
Qi Liu ◽  
Fangru Nan ◽  
...  
Keyword(s):  
Microcystis Aeruginosa ◽  
Harmful Algal Blooms ◽  
Algal Blooms ◽  
Removal Rate ◽  
Polymerase Chain Reaction Amplification ◽  
Cyanobacterial Bloom ◽  
Practical Significance ◽  
Algicidal Bacteria ◽  
Bacterial Strains ◽  
Isolation And Identification

Algae blooms present an environmental problem worldwide. In response to the outbreak of harmful algal blooms in cyanobacteria, the role of biological control has drawn wide attention, particularly for algicidal bacteria. The mechanism underlying algicidal activity was determined in our study. Algae-lysing bacteria used were separated from water and sediment collected from the Fenhe scenic spot of Taiyuan. Genetic and molecular identification was conducted by polymerase chain reaction amplification based on 16S rDNA gene. These bacterial strains were identified as Raoultella planticola and Aeromonas sp. The algae-lysing characteristics were evaluated on Microcystis aeruginosa. For the two algicidal bacteria, the high inoculation ratio (>8%) of bacteria strains contributed to the lytic effect. M. aeruginosa could be completely removed by these strains at different cell ages. However, the time used decreased with an increase in cell age. The removal rate was increased while M. aeruginosa was in the lag and logarithmic phases. The earlier bacteria strains could be inoculated, the sooner all algae could be removed. Both algicidal substances were protein, which could destroy the photosynthetic systems and break the cell of M. aeruginosa. The algicidal bacteria strain has important theoretical and practical significance for economic and feasible algae removal and provides good germplasm resources and technical support for the control of cyanobacterial bloom.

scholarly journals Adsorption Strategy for Removal of Harmful Cyanobacterial Species Microcystis aeruginosa Using Chitosan Fiber

2020 ◽  
Vol 12 (11) ◽  
pp. 4587 ◽  
Author(s):  
Yun Hwan Park ◽  
Sok Kim ◽  
Ho Seon Kim ◽  
Chulhwan Park ◽  
Yoon-E Choi
Keyword(s):  
Microcystis Aeruginosa ◽  
Harmful Algal Blooms ◽  
Algal Blooms ◽  
Control Group ◽  
Environmental Damage ◽  
Environment And Public Health ◽  
Predominant Species ◽  
Cyanobacterial Species ◽  
Cyanobacterial Harmful Algal Blooms ◽  
Chitosan Fiber

Microcystis aeruginosa is one of the predominant species responsible for cyanobacterial-harmful algal blooms (Cyano-HABs) in water bodies. Cyano-HABs pose a growing number of serious threats to the environment and public health. Therefore, the demand for developing safe and eco-friendly solutions to control Cyano-HABs is increasing. In the present study, the adsorptive strategy using chitosan was applied to remove M. aeruginosa cells from aqueous phases. Using a simple immobilization process, chitosan could be fabricated as a fiber sorbent (chitosan fiber, CF). By application of CF, almost 89% of cyanobacterial cells were eliminated, as compared to those in the control group. Field emission scanning electron microscopy proved that the M. aeruginosa cells were mainly attached to the surface of the sorbent, which was correlated well with the measurement of the surface area of the fiber. We tested the hypothesis that massive applications of the fabricated CF to control Cyano-HABs might cause environmental damage. However, the manufactured CF displayed negligible toxicity. Moreover, we observed that the release of cyanotoxins and microcystins (MCs), during the removal process using CF, could be efficiently prevented by a firm attachment of the M. aeruginosa cells without cell lysis. Our results suggest the possibility of controlling Cyano-HABs using a fabricated CF as a non-toxic and eco-friendly agent for scaled-up applications.

Flocculation Removal of Microcystis Aeruginosa by Chitosan-Bentonite Compound Material

2011 ◽  
Vol 335-336 ◽  
pp. 1381-1384
Author(s):  
Bin Guo Zheng ◽  
Wei Gong Peng ◽  
Ji Biao Zhang ◽  
Zheng Zheng
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Water Treatment ◽  
Adverse Effects ◽  
Microcystis Aeruginosa ◽  
Harmful Algal Blooms ◽  
Algal Blooms ◽  
Drinking Water Treatment ◽  
Treatment Processes ◽  
Compound Material

Harmful algal blooms have long been an issue worldwide owing to their adverse effects on drinking water treatment processes as well as drinking water quality. In this paper, chitosan-bentonite compound material was prepared by the supporting of chitosan on pillared bentonite and used for removal of harmful algae from water. The results showed that the compound material was effective for the removal of cyanobacterial Microcystis aeruginosa.

scholarly journals Genomic insight for Algicidal activity in Rhizobium sp. (AQ_MP)

2021 ◽  
Author(s):  
Mili Pal ◽  
Asifa Qureshi ◽  
Hemant Purohit
Keyword(s):  
Microcystis Aeruginosa ◽  
Harmful Algal Blooms ◽  
Algal Blooms ◽  
Nutrient Release ◽  
Glycoside Hydrolases ◽  
Polysaccharide Lyases ◽  
Responsible Gene ◽  
Wide Range ◽  
Biological Detoxification ◽  
Rhizobium Sp

Abstract Occurrence of Harmful Algal Blooms (HABs) creates a threat to aquatic ecosystem affecting the existing flora and fauna. Hence, the mitigation of HABs through an eco-friendly approach remains a challenge for environmentalists. The present study provides the genomic insights of Rhizobium sp. (AQ_MP), an environmental isolate that showed the capability of degrading Microcystis aeruginosa (Cyanobacteria) at laboratory scale. Genome sequence analysis of Rhizobium sp. (AQ_MP) was performed to determine the algal lysis properties and toxin degradative pathway. It is envisaged that Rhizobium sp. (AQ_MP) secreted CAZymes like Glycosyltransferases (GT), Glycoside Hydrolases (GH), polysaccharide lyases (PL), which allowed algal polysaccharide degradation (lysis) and enabled nutrient release for the subsequent growth of Rhizobium sp. (AQ_MP) Genome analysis also showed the presence of the glutathione metabolic pathway, which is the biological detoxification pathway responsible for microcystin degradation. The conserved region mlrC, a microcystin toxin degrading responsible gene, was also annotated in Rhizobium sp. (AQ_MP). This study confirmed that Rhizobium sp. (AQ_MP) harbours a wide range of crucial enzymes released for lysis of Microcystis aeruginosa (M. aeruginosa) cells and also for degradation of microcystin toxin. This study thus find promiscuity for scaling the lab based analysis to field level in future.

Sign in / Sign up

Export Citation Format

Share Document