Allozyme variation in populations of Lacerta raddei and Lacerta nairensis (Sauria: Lacertidae) from Armenia

1996 ◽  
Vol 17 (3) ◽  
pp. 233-246 ◽  
Author(s):  
Darlene E. Upton ◽  
Felix D. Danielyan ◽  
Michèle L. Bobyn ◽  
Ilya S. Darevsky ◽  
Robert W. Murphy ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mitochondrial Dna ◽  
Allozyme Variation ◽  
The Other ◽  
Separate Species ◽  
Allelic Differences ◽  
Allozyme Loci ◽  
And Behavior

AbstractLacertca raddei and Lacerta nairensis have been recognized as two separate species based on morphology and behavior, and each has been implicated as a sexual parent of different parthenogenetic forms. However, recent mitochondrial DNA work failed to distinguish these two as separate species. We examined genetic diversity at 36 allozyme loci from six populations of L. nairensis and four populations of L. raddei. There were no fixed allelic differences between the two. Mean heterozygosity was slightly higher among populations of L. raddei than among populations of L. nairensis. A Distance Wagner phenogram showed that the northernmost population of L. raddei clustered with the L. nairensis populations; the other L. raddei populations clustered together. We suggest that L. raddei and L. nairensis may not be separate species, a finding which has important implications for determining the origins of some parthenogenetic Lacerta.

Allozyme Variation and Population Substructuring in the Caucasian Ground Lizards Lacerta derjugini and Lacerta praticola

2011 ◽  
Vol 4 (2) ◽  
pp. 115-119 ◽  
Author(s):  
Ross D. MacCulloch ◽  
Ilya S. Darevsky ◽  
Robert W. Murphy ◽  
Jinzhong Fu
Keyword(s):  
Genetic Diversity ◽  
Allozyme Variation ◽  
Close Proximity ◽  
Geographic Separation ◽  
Population Substructuring ◽  
Allozyme Loci ◽  
Two Populations

Genetic diversity at 35 allozyme loci was surveyed in Lacerta derjugini (3 populations) and L. praticola (2 populations). Indices of variability were consistent with those found in other Caucasian Lacerta. There was little genetic substructuring between two populations of L. praticola despite considerable geographic separation. Conversely, populations of L. derjugini in close proximity to one another exhibited considerable substructuring.

LACK OF CLONAL VARIATION AMONG CANADIAN POPULATIONS OF THE CORN LEAF APHID, RHOPALOSIPHUM MAIDIS FITCH (HOMOPTERA: APHIDIDAE)

1995 ◽  
Vol 127 (5) ◽  
pp. 623-629 ◽  
Author(s):  
J.C. Simon ◽  
P.D.N. Hebert ◽  
C. Carillo ◽  
R. De Melo
Keyword(s):  
Genetic Diversity ◽  
Mitochondrial Dna ◽  
North America ◽  
North Africa ◽  
Allozyme Variation ◽  
Clonal Variation ◽  
Allozyme Analysis ◽  
Rhopalosiphum Maidis ◽  
Mtdna Variation ◽  
Corn Leaf Aphid

AbstractThe extent of allozyme variation and the amount of genetic divergence were assessed among populations of the aphid Rhopalosiphum maidis in Ontario. Allozyme analysis of individuals from 12 sites revealed no polymorphism for 32 loci. In addition, the extent of karyotypic, allozyme, and mitochondrial DNA (mtDNA) variation was examined for clones from North America, Europe, and North Africa. As previously noted, chromosome number varied in relation to the host-plant on which aphids were collected, but neither allozyme nor mtDNA patterns allowed discrimination among clones. Further work is needed to determine whether the lack of genetic diversity in R. maidis is linked with its recent introduction to North America or with its loss of a sexual phase.

Allozyme and mitochondrial DNA separation of Pacific northern bluefin tuna, Thunnus thynnus orientalis (Temminck and Schlegel), from southern bluefin tuna, Thunnus maccoyii (Castelnau)

1995 ◽  
Vol 46 (6) ◽  
pp. 921 ◽  
Author(s):  
RD Ward ◽  
NG Elliott ◽  
PM Grewe
Keyword(s):  
Mitochondrial Dna ◽  
Fishery Management ◽  
Dna Analysis ◽  
Haplotype Frequency ◽  
Restriction Enzymes ◽  
Bluefin Tuna ◽  
Allozyme Variation ◽  
Site Variation ◽  
Southern Bluefin Tuna ◽  
Allozyme Loci

Northern and southern bluefin tunas are morphologically similar and can be misidentified, posing problems for fishery management and marketing. Allozyme variation and restriction-site variation in mitochondrial DNA (mtDNA) were used to distinguish between the two species. A survey of 36 allozyme loci active in white muscle and liver tissue showed that the genetic identity between the species was high (Nei's I = 0.907). One diagnostic locus (sAH*) and two nearly diagnostic loci (ADA* and GDA*) were found, and four loci showed highly significant allele frequency differences (FH*, GPI-A*, PGDH* and sSOD*). A survey of the mtDNA genome, using 15 restriction enzymes and southern blotting, revealed five restriction enzymes that gave species-diagnostic restriction digest profiles (Ban I, Bcl I, Dra I, Pvu II, Xba I) and a further three enzymes (Pst I, Barn HI and Nco I) with large haplotype frequency differences. Mitochondrial DNA analysis provided more reliable discrimination of specimens than did allozyme analysis, although the more rapid allozyme identification will be accurate for most specimens. The two biochemical genetic methods were then used to identify Australian-caught fish of uncertain identity. Six of 12 tuna originally considered to be northern bluefin tuna were confirmed as northern bluefin and six were identified as southern bluefin. The presence of northern bluefin tuna as far south as south-western Tasmania was confirmed.

Allozyme variation and population structure of Carex humilis var. nana (Cyperaceae) in Korea

2001 ◽  
Vol 79 (4) ◽  
pp. 457-463 ◽  
Author(s):  
Man Kyu Huh
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Genetic Variation ◽  
North American ◽  
Population Level ◽  
Allozyme Variation ◽  
Population Divergence ◽  
Geographic Ranges ◽  
The Mean ◽  
Allozyme Loci

Genetic diversity and population structure of 22 Carex humilis var. nana Ohwi (Cyperaceae) populations in Korea were determined using genetic variation at 23 allozyme loci. This is a long-lived herbaceous species with a widespread distribution in eastern Asia. The 12 enzymes revealed 23 putative loci, of which 11 were polymorphic (47.8%). Genetic diversity at the varietal level and at the population level was 0.131 and 0.118, respectively. Total genetic diversity (HT = 0.274) and within population genetic diversity (HS = 0.256) were high, whereas the extent of the population divergence was relatively low (GST = 0.068). An indirect estimate of the number of migrants per generation (Nm = 3.42) indicated that gene flow was high among Korean populations. Wide geographic ranges, perennial herbaceous nature, and the persistence of multiple generations are associated with the high level of genetic variation. A distinct difference between Asian and North American Carex is shown in the proportion of genetic variation (GST) (p < 0.001). The mean GST of Asian Carex was estimated as 0.056; thus, only 5.6% of genetic variability was distributed among populations, whereas the mean GST of North American Carex was estimated as 19.5% (3.5 times higher). It is probable that the geographical distance between population pairs and presence or absence of glacial history may play roles in the substantial difference between both groups.Key words: Carex humilis var. nana, genetic diversity, population structure.

scholarly journals Genetic Diversity Estimates for the Genus Hydrangea and Development of a Molecular Key Based on SSR

2006 ◽  
Vol 131 (6) ◽  
pp. 787-797 ◽  
Author(s):  
Timothy A. Rinehart ◽  
Brian E. Scheffler ◽  
Sandra M. Reed
Keyword(s):  
Genetic Diversity ◽  
Genetic Similarity ◽  
Gene Diversity ◽  
The Other ◽  
Separate Species ◽  
Low Diversity ◽  
Wide Hybrids ◽  
Diversity Estimates ◽  
Total Diversity ◽  
Size Data

Using 14 codominant microsatellite markers that amplify loci across 14 different Hydrangea L. species, we analyzed gene diversity and genetic similarity within Hydrangea. Samples also included Dichroa Lour., Platycrater Sieb. and Zucc., and Schizophragma Sieb. and Zucc. genera to establish their relatedness to Hydrangea species since previous work suggests they may be closely related. Our results support the close affiliation between Macrophyllae E.M. McClint. and Petalanthe (Maxim.) Rehder subsections and their separation from the other Hydrangea species. Most of the Hydrangea species analyzed cluster within their designated sections and subsections; however, genetic distance between species within each subsection varied considerably. Our data suggest that morphological analyses which labeled H. serrata (Thunb.) Ser. as a subspecies of H. macrophylla (Thunb. Ex J.A. Murr.) Ser. are probably more accurate than recent genome size data suggesting H. macrophylla ssp. macrophylla (Thunb.) Ser. and H. macrophylla ssp. serrata (Thunb.) Makino are separate species. Gene diversity estimates indicate that 64.7% of the total diversity is due to differences between species and 49.7% of the overall variation is due to differences between subsections. Low diversity suggests a lack of gene flow between species and subsections and underscores the difficulty in making wide hybrids. Since only 35.3% of the genetic variation is common to all species, unique alleles were used to develop a molecular key for unambiguous species identification and interspecific hybrid verification. Genetic similarity estimates for all 85 samples suggests a roadmap for introgressing horticulturally important traits from different Hydrangea species.

Allozyme variation in three closely related species of Caucasian rock lizards (Lacerta)

1995 ◽  
Vol 16 (4) ◽  
pp. 331-340 ◽  
Author(s):  
Ross D. MacCulloch ◽  
F.D. Danielyan ◽  
Ilya S. Darevsky ◽  
Robert W. Murphy ◽  
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Genetic Distance ◽  
Related Species ◽  
Allozyme Variation ◽  
Direct Count ◽  
Closely Related Species ◽  
Polymorphic Loci ◽  
Allozyme Loci ◽  
Insular Populations

AbstractGenetic diversity at 37 allozyme loci was surveyed from Lacerta valentini (4 populations), L. portschinskii and L. rudis (1 population each). The number of polymorphic loci ranged from 1 (L. valentini) to 11 (L. rudis). Mean heterozygosity (direct count) ranged from 0.003 (L. valentini) to 0.071 (L. rudis). Nei's (1978) genetic distance ranged from 0-0.03 among populations of L. valentini, 0.127-0.163 between L. valentini and L. rudis and 0.366-0.487 between L. portschinskii and the two other taxa. Indices of genetic variability for species having disjunct distributions were lower than in species with contiguous distributions, similar to the case of insular populations, which have lower values than do mainland populations.

Mitochondrial DNA and allozyme variation in Atlantic salmon (Salmo salar) populations in Wales

1995 ◽  
Vol 52 (1) ◽  
pp. 171-178 ◽  
Author(s):  
M. O'Connell ◽  
D. O. F. Skibinski ◽  
J. A. Beardmore
Keyword(s):  
Mitochondrial Dna ◽  
Atlantic Salmon ◽  
Salmo Salar ◽  
Restriction Enzymes ◽  
Allozyme Variation ◽  
Data Sets ◽  
Atlantic Salmon Salmo Salar ◽  
Length Polymorphisms ◽  
Relative Value ◽  
Allozyme Loci

Four restriction enzymes were used to assay mitochondrial DNA (mtDNA) variation in Atlantic salmon (Salmo salar) in Wales. A total of 122 fish from 22 sites, within three catchments, were analyzed. A further 368 fish from seven of these sites were also investigated at 19–21 allozyme loci. The allozyme and mtDNA data revealed significant genetic differentiation between and within catchments. The allozyme data were as informative as mtDNA restriction fragment length polymorphisms in distinguishing between populations of Atlantic salmon. The similarity between data sets in information content is in contrast with previous studies. The relative value of both techniques to fishery managers is discussed.

The genetic structure and the conservation of the five natural populations of Pinusradiata

1988 ◽  
Vol 18 (5) ◽  
pp. 506-514 ◽  
Author(s):  
G. F. Moran ◽  
J. C. Bell ◽  
K.G. Eldridge
Keyword(s):  
Genetic Diversity ◽  
Natural Populations ◽  
Allozyme Variation ◽  
Conservation Strategies ◽  
Mainland Population ◽  
Island Populations ◽  
Expected Heterozygosity ◽  
Environmental Criteria ◽  
Total Genetic Diversity ◽  
Allozyme Loci

Pinusradiata is restricted to three mainland coastal populations in California and two island populations off Baja California, Mexico. In this study each population was divided into two geographic stands based on natural disjunctions within populations. In addition to the division mentioned above, each mainland population was also divided into a number of ecological stands based on ecological and environmental criteria. Genetic variation was measured by analysing 31 allozyme loci electrophoretically using germinated seed from 272 families across all stands and populations. Moderate levels of allozyme variation were found as shown by population averages for mean number of alleles per locus (1.79), percent polymorphic loci (46.4%, 0.99 criterion), and expected heterozygosity (0.098). In fact, the overall genetic diversity in P. radiata (HT = 0.117) is low compared with that of other conifers. In comparison with populations, the levels of allozyme variation in both ecological and geographic stands were slightly lower (means across ecological stands: A = 1.56, P = 39.3%, and He = 0.095). Of the total genetic diversity in P. radiata, 16.2% could be apportioned between populations, a high proportion for a conifer. In contrast, only 2.0 and 1.3% of the genetic diversity on average within mainland populations was between ecological and geographic stands, respectively. These results indicate a low level of genetic differentiation within populations, at least based on the ecological or geographic criteria used in this study. The implications and usefulness of these findings for the development of insitu conservation strategies are discussed.

scholarly journals Morpometric study of Hungarian honey bee (Apis mellifera L.) colonies

2013 ◽  
pp. 59-63
Author(s):  
Erika Zakar ◽  
Edit Zajácz ◽  
Tímea Rácz ◽  
János Oláh ◽  
András Jávor ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mitochondrial Dna ◽  
Apis Mellifera ◽  
Honey Bee ◽  
Honey Bees ◽  
The Other ◽  
Special Importance ◽  
Agricultural Plants ◽  
The Common ◽  
The One

The honey bees are essential for the pollination of agricultural plants. The Pannonian honey bee, Apis mellifera pannonica, is native to Hungary, only these subspecies are being bread in our country. The parameters have been separated the pannon and italian honey bee subspecies, the colour of tergit, the cubital index and proboscis length. The morphometric analisys is of special importance because this, on the one hand, shows correlation with honey bee production and on the other hand, the pure morphometric charactersitics is the basis of any potential honey bee export. Mitochondrial DNA and microsatellites are the common methods to define genetic diversity and the separation of subspecies.

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