Genisys and computer-assisted identification of nematodes

Nematology ◽  
2000 ◽  
Vol 2 (1) ◽  
pp. 17-30 ◽  
Author(s):  
Jack Milton ◽  
Renaud Fortuner ◽  
Jim Diederich

AbstractIdentification of species for the study of biodiversity in the phylum Nemata is defined as being able to identify any of the 12 000 described species of nematodes, and being able to recognise when the specimen under study belongs to a species unknown to science. Moreover, this large-scale identification must be done by biologists who are not expert taxonomists. It is argued that printed identification aids are too rigid and unreliable, and molecular identification aid is too limited, to be used in this context. Only computer identification tools are flexible, reliable, and general enough to give a non-taxonomist the possibility to identify any species. However, a review of existing tools and approaches shows that they fall short of reaching this objective. The functionality of a general identification system that could overcome the problems and difficulties encountered in the use of computerised tools is then described. This system (which has been described by the authors in several articles under the name of Genisys) would include a general database with all the characters from all the published descriptions of nematodes species and a set of integrated identification tools. Some of these tools would export the data into formats usable by existing identification and systematics tools. Other tools would have to be developed to support new approaches. A guidance tool would help the non-expert user by suggesting the successive computerised tools to be used during an identification session or by directing the user to other approaches, such as molecular identification in the case of heteroderids or some Meloidogyne species. L’identification des espèces pour l’étude de la biodiversité dans le phylum Nemata est définie comme la possibilité d’identifier n’importe laquelle des 12 000 espèces décrites du groupe et d’être capable de reconnaître que le spécimen étudié appartient à une espèce non encore décrite. En outre, ce type d’identification à grande échelle doit pouvoir être pratiqué par des biologistes qui ne sont pas des taxinomistes. Il est montré que les aides imprimées à l’identification sont trop rigides et trop peu fiables et que l’identification moléculaire est encore de portée trop limitée pour être utilisables dans ce contexte. Seuls les outils d’identification assistée par ordinateur sont suffisamment flexibles, fiables et généraux pour permettre à un non taxinomiste d’accomplir les identifications définies plus haut. Pourtant, une revue des outils existants et des approches utilisées montre qu’ils sont incapables d’atteindre l’objectif fixé. La fonctionnalité d’un système d’identification général capable de résoudre les problèmes soulevés par l’utilisation d’outils informatisés est ensuite décrite. Ce système (décrit par les auteurs du présent article sous le nom de Genisys) doit comprendre une base de données avec tous les caractères inclus dans les descriptions publiées des espèces connues et un ensemble d’outils d’identification intégrés. L’un de ces outils devrait permettre d’exporter les données sous des formats utilisables par les outils existants d’identification et de systématique. D’autres outils devraient être développés pour mettre en œuvre de nouvelles approches. Un outil de guidage aiderait le non-spécialiste en lui suggérant les outils informatiques à utiliser successivement dans le cadre d’une session d’identification ou en le dirigeant vers d’autres approches telles que l’identification moléculaire dans le cas de certains Hétérodérides et d’espèces de Meloidogyne.

1993 ◽  
Vol 76 (3_suppl) ◽  
pp. 1089-1090 ◽  
Author(s):  
F. Richard Ferraro

The present article describes a demonstration experiment used in a large introductory psychology class pertaining to mental imagery ability. The experiment is effective in providing a concrete instance of mental imagery as well as an effective discussion regarding individual differences and gender differences in imagery ability.


2007 ◽  
Vol 57 (12) ◽  
pp. 2777-2789 ◽  
Author(s):  
Sabri M. Naser ◽  
Peter Dawyndt ◽  
Bart Hoste ◽  
Dirk Gevers ◽  
Katrien Vandemeulebroecke ◽  
...  

The aim of this study was to evaluate the use of the phenylalanyl-tRNA synthase alpha subunit (pheS) and the RNA polymerase alpha subunit (rpoA) partial gene sequences for species identification of members of the genus Lactobacillus. Two hundred and one strains representing the 98 species and 17 subspecies were examined. The pheS gene sequence analysis provided an interspecies gap, which in most cases exceeded 10 % divergence, and an intraspecies variation of up to 3 %. The rpoA gene sequences revealed a somewhat lower resolution, with an interspecies gap normally exceeding 5 % and an intraspecies variation of up to 2 %. The combined use of pheS and rpoA gene sequences offers a reliable identification system for nearly all species of the genus Lactobacillus. The pheS and rpoA gene sequences provide a powerful tool for the detection of potential novel Lactobacillus species and synonymous taxa. In conclusion, the pheS and rpoA gene sequences can be used as alternative genomic markers to 16S rRNA gene sequences and have a higher discriminatory power for reliable identification of species of the genus Lactobacillus.


2019 ◽  
pp. 17-37
Author(s):  
Иосиф Александрович Фридман

Статья посвящена творческому наследию выдающегося итальянско-еврейского учёного, историка и библеиста Умберто Кассуто (1883-1951). Его основной вклад в науку о Священном Писании - развёрнутая критика документальной гипотезы происхождения Пятикнижия. Основная часть данной статьи представляет собой изложение воззрений Кассуто на первый из «столпов» документальной гипотезы - употребление в тексте Торы Божественных имён. Автор надеется показать, что, используя строго научные методы, Кассуто даёт вполне правдоподобные альтернативные объяснения тем феноменам, на исключительную правоту собственного объяснения которых с момента возникновения претендует документальная гипотеза. Вместо того чтобы опровергать частные аспекты названной теории, Кассуто, последовательно выступая против самого принципа деления текста Пятикнижия на первоначально независимые друг от друга «источники», фактически предложил в библеистике новую научную парадигму, потенциально претендующую на всеохватность и полную замену документальной гипотезы. В последующей статье на эту же тему автор намеревается продемонстрировать подход У. Кассуто ко второму, третьему, четвёртому и пятому «столпам» документальной гипотезы, а также остановить внимание на рецепции идей Кассуто в мире библеистики. The present article is devoted to the scientific heritage left by a major Jewish academician of Italian descent, historian and biblical scholar Umberto Cassuto (1883-1951). His main contribution to this field consists in large-scale criticism of the Documentary Hypothesis of the origin of the Pentateuch. In the main part of our article the author presents an outline of Cassuto’s views on the first of the five «pillars» onwhich the Documentary Hypothesis rests. The author endeavors to demonstrate that, using strictly scientific argumentation, Cassuto gives quite plausible alternative explanations to such phenomena as the Documentary Hypothesis claims its unique right to explain correctly. Instead of attacking and refuting any particular aspects of the hypothesis, Cassutoconsistently argues against the very principle according to which the Torah is divided among a number of «source texts» perceived to have once been independent of one another. As a matter of fact, U. Cassuto has presented a new scholarly paradigm with a potential that would suffice to make it a full-fledged substitution of the Documentary Hypothesis. In a second article on this topic the author intends to demonstrate Cassuto’s approach to the other four «pillars» of the Documentary Hypothesis as well as to devote special attention to the response that Cassuto’s ideas met in the world of Bible studies.


2003 ◽  
Vol 93 (6) ◽  
pp. 483-490 ◽  
Author(s):  
M.E. Carew ◽  
V. Pettigrove ◽  
A.A. Hoffmann

AbstractChironomids are excellent biological indicators for the health of aquatic ecosystems, but their use at finer taxonomic levels is hindered by morphological similarity of species at each life stage. Molecular markers have the potential to overcome these problems by facilitating species identification particularly in large-scale surveys. In this study, the potential of the polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) approach was tested to rapidly distinguish among chironomids within a geographic area, by considering chironomid species from Melbourne, Australia. By comparing molecular markers with diagnostic morphological traits, RFLP profiles of the cytochrome oxidase I (COI) region were identified that were specific to genera and some common species. These profiles were used to develop an RFLP–based key, which was validated by testing the markers on samples from several wetlands and streams. As well as allowing for rapid identification of species that are difficult to separate on morphological grounds, this approach also has the potential to resolve current taxonomic ambiguities.


2019 ◽  
Author(s):  
Miguel A. Vadillo

Ego depletion has been successfully replicated in hundreds of studies. Yet the most recent large-scale Registered Replication Reports (RRR), comprising thousands of participants, have yielded disappointingly small effects, sometimes even failing to reach statistical significance. Although these results may seem surprising, in the present article I suggest that they are perfectly consistent with a long-term decline in the size of the depletion effects that can be traced back to at least 10 years ago, well before any of the RRR on ego depletion were conceived. The decline seems to be at least partly due to a parallel trend toward publishing better and less biased research.


Author(s):  
Elizabeth Hartnell-Young

This paper takes a large-scale social perspective in describing a national project in Australia that was premised on local school communities working together and contributing ideas for the benefit of their students, and potentially, the whole country. The project was intended to improve schools’ capacity for educating boys, and in the long-term, the learning outcomes of under-performing boys, using evidence-based and action research methods. It was supported by the web spaces and tools of the National Quality Schooling Framework and Think.com. This paper emphasises the structures and processes teachers engaged in while building knowledge through their daily work, where the resulting ideas became the property of the whole community. Analyses focus on the extent to which an underlying social structure for knowledge building developed in various parts of the nation during the project, making it possible to characterize a process for innovations in education with commitment to continual idea improvement. Résumé Le présent article adopte une perspective sociale à grande échelle pour décrire un projet national en Australie fondé sur la collaboration des communautés scolaires locales et leur contribution d’idées au bénéfice de leurs élèves et, éventuellement, de l’ensemble du pays. Le projet avait pour but de rendre les écoles plus aptes à éduquer les garçons et, à long terme, d’améliorer les résultats d’apprentissage des garçons qui sous-performent à l’aide de méthodes de recherche-action fondées sur des données probantes. Il a bénéficié du soutien des espaces et des outils Web du National Quality Schooling Framework et de Think.com. Le présent article met l’accent sur les structures et les processus que les enseignants ont utilisés dans leur travail quotidien pour la coélaboration de connaissances; les idées qui en ont résulté sont par la suite devenues la propriété de l’ensemble de la communauté. Les analyses portent principalement sur la mesure dans laquelle une structure sociale sous-jacente de coélaboration des connaissances s’est développée en différents endroits de la nation au cours du projet, ce qui rend possible la caractérisation d’un processus d’innovation en éducation avec un engagement envers l’amélioration continue.


Author(s):  
S. Shanawaz Basha ◽  
N. Musrat Sultana

Biometrics refers to the automatic recognition of individuals based on their physiological and/or behavioral characteristics, such as faces, finger prints, iris, and gait. In this paper, we focus on the application of finger print recognition system. The spectral minutiae fingerprint recognition is a method to represent a minutiae set as a fixedlength feature vector, which is invariant to translation, and in which rotation and scaling become translations, so that they can be easily compensated for. Based on the spectral minutiae features, this paper introduces two feature reduction algorithms: the Column Principal Component Analysis and the Line Discrete Fourier Transform feature reductions, which can efficiently compress the template size with a reduction rate of 94%.With reduced features, we can also achieve a fast minutiae-based matching algorithm. This paper presents the performance of the spectral minutiae fingerprint recognition system, this fast operation renders our system suitable for a large-scale fingerprint identification system, thus significantly reducing the time to perform matching, especially in systems like, police patrolling, airports etc,. The spectral minutiae representation system tends to significantly reduce the false acceptance rate with a marginal increase in the false rejection rate.


2020 ◽  
Author(s):  
José Massougbodji ◽  
Hervé Tchala Vignon Zomahoun ◽  
Evehouenou Lionel Adisso ◽  
Jasmine Sawadogo ◽  
Valérie Borde ◽  
...  

Abstract Background Little is known about engaging patients and stakeholders in the process of scaling up effective knowledge translation interventions targeting the general public. Using an integrated knowledge translation approach, we aimed to scale up and evaluate an effective pilot program of disseminating research results in public libraries. Methods We conducted a scaling-up study targeting the general public. Based on our successful pilot project, we co-developed and implemented a larger-scale program of free citizen workshops in public libraries, this time in close research partnership with stakeholders and patient representatives. Citizen workshops, each facilitated by one participating physician and one science communicator, consisted of a 45-min computer-assisted presentation and a 45-min open exchange. Additional scale-up costs included offering financial incentives to stakeholders involved and the purchase of audio-visual equipment. The intervention outcome was knowledge gained. Scale-up outcomes were satisfaction, appropriateness, coverage, time and costs. An evaluation questionnaire was used to collect data of interest. Both quantitative and qualitative analyses were performed. Results The workshop theme chosen by patient and stakeholder representatives was the high prevalence of medication overuse among people over 65 years of age. From April to May 2019, 26 workshops were given in 25 public libraries reaching 362 people. Eighteen participating physicians and six science communicators facilitated the workshops. Participants reported significant knowledge gain (mean difference 2.1, 95% CI 2.0–2.2, P < .001). Median score for overall public satisfaction was 9/10 (IQR 8–10). A high level of appropriateness of the workshops was globally rated by the public participants Coverage was 92.6% of the total number of public libraries targeted. Costs were $6,051.84 CAD for workshop design and $22,935.41 CAD for scaling them up. Conclusion This project successfully established a large-scale and successful KT bridge between researchers, clinicians, and citizens via public libraries. This study provides a model for a dissemination practice that benefits the general public by both engaging them in the dissemination process and by targeting them directly.


Sign in / Sign up

Export Citation Format

Share Document