Comparative morphology of leaf epidermis in 34 species of Maianthemum (Asparagaceae, Polygonateae) and their systematic significance

Phytotaxa ◽  
2016 ◽  
Vol 275 (2) ◽  
pp. 81 ◽  
Author(s):  
YING MENG ◽  
JIA-JIAN WANG ◽  
ZE-LONG NIE
Keyword(s):  
Geographic Distribution ◽  
Molecular Phylogenetics ◽  
Leaf Surface ◽  
Guard Cells ◽  
Comparative Morphology ◽  
Leaf Epidermis ◽  
Systematic Significance ◽  
Anomocytic Stomata ◽  
Epidermal Features ◽  
Scanning Electron

To investigate the possibility of using leaf epidermal characters for species identification in developing a classification for Maianthemum, we examined leaf epidermal features from across the geographic distribution of the genus, including sampling of 34 species, one subspecies and one variety, and used light and scanning electron microscopy to make observations of these features. Our results suggest that the shape of epidermal cells, and anticlinal walls on both the abaxial and adaxial leaf surface have systematic significance for defining Maianthemum taxa and are congruent with clades inferred using molecular phylogenetics. The pattern of anticlinal walls in the genus seems to be related to the geographic distribution and environmental conditions. Other characters yielded in this study including anomocytic stomata, elliptic to narrow elliptic guard cells, wrinkled, striate or nearly smooth cuticle, smooth or sinuolate to erose inner margin of the outer stomatal rim, are not useful for taxon circumscription in the genus.

scholarly journals Epidermal features of rice leaf CV. BRRI Dhan29

1970 ◽  
Vol 16 (2) ◽  
pp. 177-180 ◽  
Author(s):  
Md. Tofazzal Islam ◽  
A.K.M. Golam Sarwar ◽  
Hasna Hena Begum ◽  
Toshiaki Ito
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Leaf Epidermis ◽  
Rice Leaf ◽  
Plant Taxon ◽  
Epidermal Features ◽  
Scanning Electron

Not available.Keywords: Leaf epidermis; Rice; Scanning electron microscopy (SEM); Slender macro hair. DOI: 10.3329/bjpt.v16i2.3931 Bangladesh J. Plant Taxon. 16(2): 177-180, 2009 (December)

scholarly journals The position and structure of the nectary in spring heath (Erica carnea L.) flowers

2012 ◽  
Vol 62 (2) ◽  
pp. 13-21 ◽  
Author(s):  
Elżbieta Weryszko-Chmielewska ◽  
Mirosław Chwil ◽  
Marek Wróbel
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Guard Cells ◽  
Small Degree ◽  
Nectar Secretion ◽  
Ecological Traits ◽  
Anomocytic Stomata ◽  
Pore Opening ◽  
Floral Nectaries ◽  
Scanning Electron

Ecological traits of <i>Erica carnea</i> L. flowers and the morphology of floral nectaries were investigated using stereoscopic, light and scanning electron microscopy. The nectary in the flowers of <i>Erica carnea</i> is located in the basal part of the ovary. It represents the gynoecial nectary type. It has the form of a yellow, ribbed ring with eight outgrowths, pointed towards the base, which alternately adjoin the stamen filaments. The height of the nectary is 400 µm and its thickness 200 - 250 µm. The parenchyma of the nectary is composed of 6 - 8 layers. Nectar secretion occurs through anomocytic stomata with a diameter of 17 µm. Guard cells are only found on the outgrowths of the nectary and they are situated most frequently at the level of other epidermal cells. During nectar secretion, a small degree of pore opening was observed. In the flowers of <i>Erica carnea</i>, secondary nectar presentation was found, with the nectar accumulating at the base of the fused corolla.

A Scanning Electron Microscope Study of Isolated Guard Cells

1973 ◽  
Vol 31 ◽  
pp. 454-455 ◽  
Author(s):  
P. Dayanandan ◽  
P. B. Kaufman
Keyword(s):  
Electron Microscope ◽  
Electron Microscope Study ◽  
Three Dimensional ◽  
Guard Cells ◽  
Scanning Electron Microscope Study ◽  
Psilotum Nudum ◽  
Subsidiary Cells ◽  
Welwitschia Mirabilis ◽  
Cycas Revoluta ◽  
Scanning Electron

A three dimensional appreciation of the guard cell morphology coupled with ultrastjuctural studies should lead to a better understanding of their still obscure dynamics of movement. We have found the SEM of great value not only in studies of the surface details of stomata but also in resolving the structures and relationships that exist between the guard and subsidiary cells. We now report the isolation and SEM studies of guard cells from nine genera of plants.Guard cells were isolated from the following plants: Psilotum nudum, four species of Equisetum, Cycas revoluta, Ceratozamia sp., Pinus sylvestris, Ephedra cochuma, Welwitschia mirabilis, Euphorbia tirucalli and Allium cepa.

scholarly journals Arabidopsis NPF4.6 and NPF5.1 Control Leaf Stomatal Aperture by Regulating Abscisic Acid Transport

Genes ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 885
Author(s):  
Takafumi Shimizu ◽  
Yuri Kanno ◽  
Hiromi Suzuki ◽  
Shunsuke Watanabe ◽  
Mitsunori Seo
Keyword(s):  
Abscisic Acid ◽  
Plant Hormone ◽  
Leaf Surface ◽  
Stomatal Closure ◽  
Guard Cells ◽  
Cell Types ◽  
Acid Transport ◽  
Wild Type ◽  
Vascular Tissues ◽  
Abscisic Acid Transport

The plant hormone abscisic acid (ABA) is actively synthesized in vascular tissues and transported to guard cells to promote stomatal closure. Although several transmembrane ABA transporters have been identified, how the movement of ABA within plants is regulated is not fully understood. In this study, we determined that Arabidopsis NPF4.6, previously identified as an ABA transporter expressed in vascular tissues, is also present in guard cells and positively regulates stomatal closure in leaves. We also found that mutants defective in NPF5.1 had a higher leaf surface temperature compared to the wild type. Additionally, NPF5.1 mediated cellular ABA uptake when expressed in a heterologous yeast system. Promoter activities of NPF5.1 were detected in several leaf cell types. Taken together, these observations indicate that NPF5.1 negatively regulates stomatal closure by regulating the amount of ABA that can be transported from vascular tissues to guard cells.

Atlas of leaf surface micromorphology in Aloe L. (Asphodelaceae) from the Horn of Africa region

Phytotaxa ◽  
2021 ◽  
Vol 524 (4) ◽  
pp. 261-282
Author(s):  
ESHETU FENTAW ◽  
KELDA F.V.A. ELLIOTT ◽  
SEBSEBE DEMISSEW ◽  
DAVID CUTLER ◽  
OLWEN M. GRACE
Keyword(s):  
Species Delimitation ◽  
Plant Material ◽  
Species Complex ◽  
Leaf Surface ◽  
Detailed Comparison ◽  
Species Variation ◽  
Leaf Epidermis ◽  
Horn Of Africa ◽  
Surface Micromorphology ◽  
Leaf Surface Micromorphology

The confident identification to species rank of fragmentary and sterile plant material is often challenged by the absence of diagnostic characters, which are present in intact specimens, reproductive parts, and plants in habitat. Here, we consider leaf surface micromorphology for the identification of the genus Aloe in the Horn of Africa region. Primary and secondary sculpturing of the leaf epidermis and stomata were characterised from SEM micrographs of 35 taxa representing 31 species of Aloe (Asphodelaceae subfam. Alooideae). Detailed comparison revealed that leaf surface characters are conserved between species and within-species variation is modest. Closely related taxa in the Aloe adigratana—A. camperi—A. sinana species complex could be distinguished using leaf surface micromorphology alone. These characters also guide species delimitation; in the species complex including A. schoelleri and A. steudneri, a narrow circumscription is supported, whereas with A. ankoberensis and A. pulcherrima, a wider circumscription merits consideration. The observed trait combinations are characteristic of plants in xeric environments, with the most notable feature being stomata that are most deeply sunken in species in more arid habitats. Our findings support the use of comparative study of micromorphological leaf surface characters for species identification and taxonomy in the genus Aloe.

scholarly journals Leaf surface characters applied to Lauraceae taxonomy in a Seasonal Forest of Southern Brazil

2009 ◽  
Vol 52 (6) ◽  
pp. 1453-1460 ◽  
Author(s):  
Guilherme Bordignon Ceolin ◽  
Jumaida Maria Rosito ◽  
Thais Scotti do Canto-Dorow
Keyword(s):  
Morphological Analysis ◽  
Leaf Surface ◽  
Guard Cells ◽  
Southern Brazil ◽  
Diagnostic Characters ◽  
Microscopical Analysis ◽  
Diagnostic Characteristics ◽  
Microscopic Evaluation ◽  
Printing Technique ◽  
Seasonal Forests

The goal of this work was to test if the macro and micro morphological analysis of the leaf surface could provide vegetative diagnostic characters for some of the most common Lauraceae species in Southern Brazilian Seasonal Forests. The leaf printing technique with universal instantaneous adhesive was used for the epidermical microscopical analysis and external macroscospical analyses of leave were made. Microscopic evaluation revealed the visibility and contours of anticlinal walls of epidermical cell and stomata and shape of guard-cells. Macroscopic evaluation showed the absence or presence of characters such as hairiness, domatia and scents. The results showed that analyzed characters, together with other diagnostic characteristics, could contribute in taxonomic delimitation of some common Lauraceae species in the Southern Brazil.

scholarly journals Petal and Sepal Epidermal Micromorphology of Six Lathyrus Taxa (Fabaceae) and their Systematic Value

2012 ◽  
Vol 40 (1) ◽  
pp. 35 ◽  
Author(s):  
Hüseyin CILDIR ◽  
Ahmet KAHRAMAN ◽  
Musa DOGAN
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Glandular Trichomes ◽  
Systematic Significance ◽  
Trichome Types ◽  
First Time ◽  
Scanning Electron

In this study, the epidermal types and their distribution on dorsal and lateral petals, the trichome types and their density on sepals of Lathyrus chloranthus, L. digitatus, L. laxiflorus subsp. laxiflorus, L. roseus subsp. roseus, L. sativus, and L. tuberosus, belonging to sections Lathyrus, Lathyrostylis, Pratensis, and Orobon of the genus Lathyrus in Turkey were investigated using light microscopy (LM) and scanning electron microscopy (SEM), and the systematic significance of these characters was evaluated. These taxa, except L. sativus, are studied for the first time under aspects of the petal and sepal micromorphology. Three major epidermal types were recognized on the petal: tabular rugose striate cells (TRS), areolate cells with more or less striations (AS), and papillose conical striate cells (PCS). TRS and AS were further subdivided into three subtypes. TRS was found on the dorsal and lateral petals of L. chloranthus, L. sativus, and L. tuberosus: the dorsal petals of L. roseus subsp. roseus and the lateral petals of L. laxiflorus subsp. laxiflorus, while AS was present on the dorsal and lateral petals of L. digitatus and the lateral petals of L. roseus subsp. roseus. PCS was found only on the dorsal petals of L. digitatus. Three main types of trichomes on the sepal were observed: peltate glandular, capitate glandular, and nonglandular trichomes. The capitate glandular and nonglandular trichomes were further subdivided into three subtypes. The peltate glandular trichomes were present only in L. chloranthus, but absent in the others. The capitate glandular trichomes were found in L. chloranthus, L. laxiflorus subsp. laxiflorus, L. roseus subsp. roseus, and L. tuberosus. The nonglandular trichomes were always present in L. chloranthus and L. laxiflorus subsp. laxiflorus. The present results show that the petal and sepal micromorphology can be used in delimitation of the taxa based on petal and sepal micromorphology.

scholarly journals Stomatal Development in Aerial Axes of Psilotum nudum (Psilotaceae)

2012 ◽  
Vol 128 (3-4) ◽  
pp. 95-99 ◽  
Author(s):  
James E. Mickle ◽  
Maria Rosaria Barone Lumaga ◽  
Paolo De Luca
Keyword(s):  
Electron Microscopy ◽  
Guard Cells ◽  
Cytoplasmic Protein ◽  
Stomatal Development ◽  
Wax Deposition ◽  
Psilotum Nudum ◽  
Pd Alloy ◽  
Entire Cell ◽  
Mother Cells ◽  
Scanning Electron

Abstract Apical regions of developing aerial shoots of Psilotum nudum (L.) Beauv. were studied using both scanning electron microscopy (SEM) and light microscopy (LM) with the aim of improving our understanding of early stages in stomatal and epidermal ontogenesis. SEM samples were fixed in gluteraldehyde, critical point dried, and coated with an Au-Pd alloy. LM samples were fixed in FAA and embedded in paraffin. LM sections were stained with 0.05% toluidine blue for protein. SEM shows that P. nudum stomata develop from 20 µm-long domed meristemoid cells into guard cell mother cells (GMCs). A furrow dividing guard cells develops at 30 µm long, and wax deposition that will cover the entire cell begins at 70 µm long. LM longitudinal sections of GMCs show a cytoplasmic protein net that organizes into radial fibers, similar to reports of actin fibers in stomata of angiosperms. This study provides additional details of stomatal development in Psilotum and is the first report of an actin-like protein net in Psilotum.

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