scholarly journals Antimicrobial Activities of Lactic Acid Bacteria Strains Isolated from Human Breast Milk Against Human Pathogenic Strains

Author(s):  
Maryam A. S. Abubakr
2018 ◽  
Vol 31 (2) ◽  
pp. 31
Author(s):  
Yusra M.B. Muhsin ◽  
Huda Z. Majeed ◽  
Basam Basim Mohammed ◽  
Salih A.A. Mohammed

     Biosurfactants have a wide-range of applications due to their unique properties like specificity, not toxicity (from LAB) and relative ease of preparation. These properties hold promise of biosurfactants to increase breast milk benefit were isolated and described into Lactobacillus plantarum, Lactobacillus fermentum ,Lactococcuslactis, and Leuconostocmesenteroides.The degree of microbial destruction of disease, which promotes  the effective remediation of disease spreading.This paper presents a review of available research, methods and publications regarding Biosurfactant extraction from Lactic Acid bacteria isolated from human breast milk. 3 samples of human breast milk was provided, LAB were isolated and described, Biosurfactants recovery and surface activity were tested and extracted endo and extra cellular. In other side 26 samples from eye patients were ordered, diagnosed and their sensitivity to biosurfactant were studied. The results showed that 5 isolates of LAB from human breast milk were biosurfactant producer but L. plantarum was the more efficiency in surface activity. In other side, out of 26 eyes sample 18 were positive to pathogenic bacteria included E.coli (7), Klebseilla (5), Staphylococcus aureus(3)and S.epidermides (3).Extracellular Biosurfactanthad good effect against tested bacteria, but endocellular (extraction by normal method) had not any effect against any bacteria, whereas by solvents method were the more active against all tested bacteria. The results are promising enough to continue the quest for enhancement of inhibition growth of pathogenic bacteria with biosurfactant application (extracted extracellular by solvents) to look forward for biosurfactant as a solution of antibiotic resistance problem. In this study we concluded thatL. plantarum was the more effectiveness in biosurfactant surface activity and the extracellular biosurfactant by solvent method for extraction were better than endocellular and normal method of extraction.


2014 ◽  
Author(s):  
Nayara Alves Reis ◽  
Norma Suely Evangelista-Barreto ◽  
Margarete Alice Fontes Saraiva ◽  
Marly Silveira Santos ◽  
Brenda Borges Vieira ◽  
...  

2021 ◽  
pp. 105297
Author(s):  
Ghulam Ayesha Javed ◽  
Najma Arshad ◽  
Aneela Munir ◽  
Shafaat Yar Khan ◽  
Sana Rasheed ◽  
...  

2007 ◽  
Vol 146 (1) ◽  
pp. 225-232 ◽  
Author(s):  
Oyuna V. Tsydenova ◽  
Agus Sudaryanto ◽  
Natsuko Kajiwara ◽  
Tatsuya Kunisue ◽  
Valeriy B. Batoev ◽  
...  

2004 ◽  
Vol 287 (5) ◽  
pp. C1404-C1411 ◽  
Author(s):  
Ryoko Minekawa ◽  
Takashi Takeda ◽  
Masahiro Sakata ◽  
Masami Hayashi ◽  
Aki Isobe ◽  
...  

Neonatal necrotizing enterocolitis (NEC), which is a disease with a poor prognosis, is considered to be caused by the coincidence of intestinal ischemia-reperfusion injury and systemic inflammation due to the colonization of pathogenic bacteria. Interleukin (IL)-8, a proinflammatory cytokine, plays an important role in the pathophysiology of NEC. It was recently reported that IL-1β activates the IL-8 gene by regulating the transcriptional nuclear factor κB (NF-κB) signaling pathways in intestinal cells. The protective role of maternal milk in NEC pathogenesis has been reported in both human and animal studies. In this study, we show that human breast milk dramatically suppressed the IL-1β-induced activation of the IL-8 gene promoter by inhibiting the activation pathway of NF-κB. Moreover, we also show that human breast milk induced the production of IκBα. These results suggest that human breast milk could be protective and therapeutic in neonates with NEC by inhibiting the activation pathway of NF-κB.


Nutrients ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 573
Author(s):  
Naoko Tsugawa ◽  
Mayu Nishino ◽  
Akiko Kuwabara ◽  
Honami Ogasawara ◽  
Maya Kamao ◽  
...  

Background: Breast milk is considered the optimal source of nutrition during infancy. Although the vitamin D concentration in human breast milk is generally considered poor for infants, vitamin D in breast milk is an important source for exclusively breastfed infants. Increases in vitamin D insufficiency and deficiency in lactating mothers may reduce vitamin D concentrations in breast milk. This study aimed to compare vitamin D and 25-hydroxyvitamin D (25OHD) concentrations in breast milk collected in 1989 and 2016–2017 and simultaneously analyze them with liquid chromatography-tandem mass spectrometry (LC-MS/MS); the association between the lifestyle of recent lactating mothers (2016–2017) and vitamin D status in human breast milk was also evaluated. Method: Lactating mothers were recruited from three regions of Japan in 1989 (n = 72) and 2016–2017 (n = 90), and milk from 3–4 months was collected in summer and winter. The samples were strictly sealed and stored at −80℃ until measurement. Breast milk vitamin D and 25OHD concentrations were analyzed by LC-MS/MS. Vitamin D intake, sun exposure, and sunscreen use of the lactating mothers in 2016–2017 were assessed. Results: Both vitamin D and 25OHD concentrations in breast milk were higher in the summer regardless of the survey year. Significantly lower vitamin D and 25OHD concentrations were observed in 2016–2017 compared with 1989 in summer, but no survey year difference was observed in winter. The stepwise multiple regression analyses identified season, daily outdoor activity, and suntan in the last 12 months as independent factors associated with vitamin D3 concentrations. Conclusion: The results suggest that low vitamin D status in recent lactating mothers may have decreased vitamin D and 25OHD concentrations in breast milk compared with the 1980s. These results are helpful for developing public health strategies to improve vitamin D status in lactating mothers and infants.


Author(s):  
Yahya R. Tahboub ◽  
Adnan M. Massadeh ◽  
Nihaya A. Al-sheyab ◽  
Diab El shrafat ◽  
Israa A. Nsserat

Sign in / Sign up

Export Citation Format

Share Document