Anti-Angiogenic and Anti-Cancer Evaluation of Betulin Nanoemulsion in Chicken Chorioallantoic Membrane and Skin Carcinoma in Balb/c Mice

2013 ◽  
Vol 9 (4) ◽  
pp. 577-589 ◽  
Author(s):  
Cristina A. Dehelean ◽  
Stefana Feflea ◽  
Dorina Gheorgheosu ◽  
Srinivas Ganta ◽  
Anca M. Cimpean ◽  
...  
Keyword(s):  
Chorioallantoic Membrane ◽  
Skin Carcinoma ◽  
Anti Cancer ◽  
Chicken Chorioallantoic Membrane

scholarly journals The Aqueous Soluble Polyphenolic Fraction ofPsidium guajavaLeaves Exhibits Potent Anti-Angiogenesis and Anti-Migration Actions on DU145 Cells

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Chiung-Chi Peng ◽  
Chiung-Huei Peng ◽  
Kuan-Chou Chen ◽  
Chiu-Lan Hsieh ◽  
Robert Y. Peng
Keyword(s):  
Chorioallantoic Membrane ◽  
Psidium Guajava ◽  
Elisa Assay ◽  
Scratch Assay ◽  
Anti Cancer ◽  
Assay Methods ◽  
Du145 Cells ◽  
Chicken Chorioallantoic Membrane ◽  
Chorioallantoic Membrane Assay ◽  
Wound Scratch Assay

The aqueous extract ofPsidium guajavabudding leaves (PE) bears an extremely high content of polyphenolic and isoflavonoids. Whether it could be used as an anti-tumor chemopreventive in view of anti-angiogenesis and anti-migration, we performed the assay methods including the MTT assay to examine the cell viability; the ELISA assay to test the expressions of VEGF, IL-6 and IL-8; the western blot analysis to detect TIMP-2; the gelatinolytic zymography to follow the expression of MMPs; the wound scratch assay to examine the migration capability; and the chicken chorioallantoic membrane assay to detect the suppressive angiogenesis. Results indicated that the IC50 of PE for DU145 cells was ∼0.57 mg ml−1. In addition, PE effectively inhibited the expressions of VEGF, IL-6 and IL-8 cytokines, and MMP-2 and MMP-9, and simultaneously activated TIMP-2 and suppressed the cell migration and the angiogenesis. Conclusively, PE potentially possesses a strong anti-DU145 effect. Thus, clinically it owns the potential to be used as an effective adjuvant anti-cancer chemopreventive.

Inhibition by doxycycline of angiogenesis in the chicken chorioallantoic membrane (CAM)

2005 ◽  
Vol 56 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Mary Richardson ◽  
David Wong ◽  
Samantha Lacroix ◽  
Jolanta Stanisz ◽  
Gurmit Singh
Keyword(s):  
Chorioallantoic Membrane ◽  
Chicken Chorioallantoic Membrane

scholarly journals Angiotropism of Human Melanoma: Studies Involving In Transit and Other Cutaneous Metastases and the Chicken Chorioallantoic Membrane

2006 ◽  
Vol 28 (3) ◽  
pp. 187-193 ◽  
Author(s):  
Claire Lugassy ◽  
Stephen E. Vernon ◽  
Klaus Busam ◽  
Jean A. Engbring ◽  
Danny R. Welch ◽  
...  
Keyword(s):  
Chorioallantoic Membrane ◽  
Human Melanoma ◽  
Cutaneous Metastases ◽  
In Transit ◽  
Chicken Chorioallantoic Membrane

Role of AKT/PKB signaling in fibroblast growth factor-1 (FGF-1)-induced angiogenesis in the chicken chorioallantoic membrane (CAM)

2004 ◽  
Vol 94 (1) ◽  
pp. 109-116 ◽  
Author(s):  
Reza Forough ◽  
Brian Weylie ◽  
Chirag Patel ◽  
Sandy Ambrus ◽  
Ugra S. Singh ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Chorioallantoic Membrane ◽  
Fibroblast Growth ◽  
Chicken Chorioallantoic Membrane

scholarly journals An antagonist of integrin alpha v beta 3 prevents maturation of blood vessels during embryonic neovascularization

1995 ◽  
Vol 108 (7) ◽  
pp. 2655-2661 ◽  
Author(s):  
C.J. Drake ◽  
D.A. Cheresh ◽  
C.D. Little
Keyword(s):  
Experimental Data ◽  
Monoclonal Antibody ◽  
Blood Vessels ◽  
Chorioallantoic Membrane ◽  
Normal Pattern ◽  
Basal Surface ◽  
Lumen Formation ◽  
Vessel Development ◽  
Alpha V Beta 3 ◽  
Chicken Chorioallantoic Membrane

Experimental data in this study demonstrate that integrin alpha v beta 3 is fundamentally involved in the maturation of blood vessels during embryonic neovascularization (vasculogenesis). Integrin alpha v beta 3 was specifically expressed on the surface of angioblasts during vessel development in quail embryos and vitronectin, a ligand for alpha v beta 3, localized to the basal surface of these cells. More importantly, microinjection of the anti-alpha v beta 3 monoclonal antibody, LM609, disrupted the normal pattern of vascular development. After exposure to LM609 the angioblasts in experimental embryos appeared as clusters of rounded cells lacking normal cellular protrusions. This led to disruption of lumen formation and abnormal vessel patterning. These findings demonstrate that during vasculogenesis ligation of integrin alpha v beta 3 on the surface of primordial endothelial cells is critical for the differentiation and maturation of blood vessels. Similar studies on chicken chorioallantoic membrane showed that LM609 blocks angiogenesis. Together the two studies suggest that integrin alpha v beta 3 plays a role in neovascularization of tissues.

scholarly journals STAT3/5 Inhibitors Suppress Proliferation in Bladder Cancer and Enhance Oncolytic Adenovirus Therapy

2020 ◽  
Vol 21 (3) ◽  
pp. 1106 ◽  
Author(s):  
Sruthi V. Hindupur ◽  
Sebastian C. Schmid ◽  
Jana Annika Koch ◽  
Ahmed Youssef ◽  
Eva-Maria Baur ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Cell Cycle Progression ◽  
Chorioallantoic Membrane ◽  
Oncolytic Adenovirus ◽  
Cycle Progression ◽  
Cellular Processes ◽  
Division Cell ◽  
Chicken Chorioallantoic Membrane ◽  
Cam Model ◽  
Phosphorylated Stat3

The JAK-STAT signalling pathway regulates cellular processes like cell division, cell death and immune regulation. Dysregulation has been identified in solid tumours and STAT3 activation is a marker for poor outcome. The aim of this study was to explore potential therapeutic strategies by targeting this pathway in bladder cancer (BC). High STAT3 expression was detected in 51.3% from 149 patient specimens with invasive bladder cancer by immunohistochemistry. Protein expression of JAK, STAT and downstream targets were confirmed in 10 cell lines. Effects of the JAK inhibitors Ruxolitinib and BSK-805, and STAT3/5 inhibitors Stattic, Nifuroxazide and SH-4-54 were analysed by cell viability assays, immunoblotting, apoptosis and cell cycle progression. Treatment with STAT3/5 but not JAK1/2 inhibitors reduced survival, levels of phosphorylated STAT3 and Cyclin-D1 and increased apoptosis. Tumour xenografts, using the chicken chorioallantoic membrane (CAM) model responded to Stattic monotherapy. Combination of Stattic with Cisplatin, Docetaxel, Gemcitabine, Paclitaxel and CDK4/6 inhibitors showed additive effects. The combination of Stattic with the oncolytic adenovirus XVir-N-31 increased viral replication and cell lysis. Our results provide evidence that inhibitors against STAT3/5 are promising as novel mono- and combination therapy in bladder cancer.

scholarly journals Tumor grafts grown on the chicken chorioallantoic membrane are distinctively characterized by MRI under functional gas challenge

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Conny F. Waschkies ◽  
Fatma Kivrak Pfiffner ◽  
Dorothea M. Heuberger ◽  
Marcel A. Schneider ◽  
Yinghua Tian ◽  
...  
Keyword(s):  
Chorioallantoic Membrane ◽  
Chicken Chorioallantoic Membrane
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