Bactericidal Effect of Silver Nanoparticles Against Mycobacterium tuberculosis

2013 ◽  
Vol 7 (3) ◽  
pp. 282-287 ◽  
Author(s):  
V. Lakshmi Praba ◽  
M. Kathirvel ◽  
K. Vallayyachari ◽  
K. Surendar ◽  
M. Muthuraj ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Mycobacterium Tuberculosis ◽  
Bactericidal Effect

scholarly journals Biosynthesis, Characterization, and Biological Activities of Procyanidin Capped Silver Nanoparticles

2020 ◽  
Vol 11 (3) ◽  
pp. 66
Author(s):  
Umar M. Badeggi ◽  
Jelili A. Badmus ◽  
Subelia S. Botha ◽  
Enas Ismail ◽  
Jeanine L. Marnewick ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Food Packaging ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Bacterial Species ◽  
Bactericidal Effect ◽  
Total Extract ◽  
Selected Area Electron Diffraction ◽  
Ic50 Values ◽  
Inhibitory Activities

In this study, procyanidin dimers and Leucosidea sericea total extract (LSTE) were employed in the synthesis of silver nanoparticles (AgNPs) and characterized by ultraviolet-visible (UV-Visible) spectroscopy, high-resolution transmission electron microscopy (HRTEM), selected area electron diffraction (SAED), X-ray diffraction (XRD), and dynamic light scattering (DLS) techniques. AgNPs of about 2–7 nm were obtained. DLS and stability evaluations confirmed that the AgNPs/procyanidins conjugates were stable. The formed nanoparticles exhibited good inhibitory activities against the two enzymes studied. The IC50 values against the amylase enzyme were 14.92 ± 1.0, 13.24 ± 0.2, and 19.13 ± 0.8 µg/mL for AgNPs coordinated with LSTE, F1, and F2, respectively. The corresponding values for the glucosidase enzyme were 21.48 ± 0.9, 18.76 ± 1.0, and 8.75 ± 0.7 µg/mL. The antioxidant activities were comparable to those of the intact fractions. The AgNPs also demonstrated bacterial inhibitory activities against six bacterial species. While the minimum inhibitory concentrations (MIC) of F1-AgNPs against Pseudomonas aeruginosa and Staphylococcus aureus were 31.25 and 15.63 µg/mL respectively, those of LSTE-AgNPs and F2-AgNPs against these organisms were both 62.50 µg/mL. The F1-AgNPs demonstrated a better bactericidal effect and may be useful in food packaging. This research also showed the involvement of the procyanidins as reducing and capping agents in the formation of stable AgNPs with potential biological applications.

The bactericidal effect of silver nanoparticles

2005 ◽  
Vol 16 (10) ◽  
pp. 2346-2353 ◽  
Author(s):  
Jose Ruben Morones ◽  
Jose Luis Elechiguerra ◽  
Alejandra Camacho ◽  
Katherine Holt ◽  
Juan B Kouri ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Bactericidal Effect

Study on the synthesis of antibacterial plastic by using silver nanoparticles doped in zeolite framework

2021 ◽  
Vol 6 (1) ◽  
pp. 32-36
Author(s):  
Anh Quoc Le ◽  
Van Phu Dang ◽  
Ngoc Duy Nguyen ◽  
Kim Lan Nguyen Thi ◽  
Kim Lang Vo Thi ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Average Particle Size ◽  
Bactericidal Effect ◽  
Ethanol Solution ◽  
Average Particle ◽  
Γ Irradiation ◽  
Zeolite Framework ◽  
Ion Exchange Reaction ◽  
Transmission Electron

Silver nanoparticles (AgNPs) doped in the zeolite framework (AgNPs/Z) were successfully synthesized by γ-irradiation in ethanol solution of silver ion-zeolite (Ag+/Z) prepared by ion exchange reaction between silver nitrate (AgNO3) and zeolite 4A. The effects of the Ag+ concentration and irradiation dose on the formation of AgNPs/Z were also investigated. AgNPs/Z with the silver content of about 10,000 ppm and the average particle size of AgNPs of about 27 nm was characterized by ultraviolet-visible spectroscopy, powder X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM). Firstly, AgNPs/Z was added into PP resins for creation of PP-AgNPs/Z masterbatch (Ag content of ~10.000 ppm) and then PP-AgNPs/Z plastics were preapared by mixing masterbatch with PP resins. The antibacterial activity of the PP-AgNPs/Z plastics was investigated against Gram-negative bacteria Escherichia coli (E. coli). The results showed that PP-AgNPs/Z plastic contained 100 ppm of Ag possessed a high antibacterial property, namely the bactericidal effect was more than 96 % on the platic surface. In conclusion, possessing many advantages such as: vigorously antibacterial effect and good dispersion in plastic matrix, AgNPs/Z is promising to be applied as bactericidal agent for plastic industry.

scholarly journals Antimicrobial Efficacy of Low Concentration PVP-Silver Nanoparticles Deposited on DBD Plasma-Treated Polyamide 6,6 Fabric

Coatings ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 581 ◽  
Author(s):  
Ana Isabel Ribeiro ◽  
Dilara Senturk ◽  
Késia Karina Silva ◽  
Martina Modic ◽  
Uros Cvelbar ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Low Cost ◽  
Antimicrobial Effect ◽  
Bactericidal Effect ◽  
Dbd Plasma ◽  
Low Concentration ◽  
Log Reduction ◽  
Spray Method ◽  
Antibacterial Textiles ◽  
Fabric Surface

In this study, a low concentration (10 μg·mL−1) of poly(N-vinylpyrrolidone) (PVP)-coated silver nanoparticles (AgNPs) were deposited by spray and exhaustion (30, 70 and 100 °C) methods onto untreated and dielectric barrier discharge (DBD) plasma-treated polyamide 6,6 (PA66) fabric. DBD plasma-treated samples showed higher AgNP deposition than untreated ones for all methods. After five washing cycles, only DBD plasma-treated samples displayed AgNPs on the fabric surface. The best-performing method was exhaustion at 30 °C, which exhibited less agglomeration and the best antibacterial efficacy against S. aureus (4 log reduction). For E. coli, the antimicrobial effect showed good results in all the exhaustion samples (5 log reduction). Considering the spray method, only the DBD plasma-treated samples showed some bacteriostatic activity for both strains, but the AgNP concentration was not enough to have a bactericidal effect. Our results suggest DBD plasma may be a low cost and chemical-free method for the preparation of antibacterial textiles, allowing for the immobilization of a very low—but effective—concentration of AgNPs.

scholarly journals In VitroCombination Studies of Benzothiazinone Lead Compound BTZ043 against Mycobacterium tuberculosis

2012 ◽  
Vol 56 (11) ◽  
pp. 5790-5793 ◽  
Author(s):  
Benoit Lechartier ◽  
Ruben C. Hartkoorn ◽  
Stewart T. Cole
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Inhibitory Concentration ◽  
Bactericidal Effect ◽  
Lead Compound ◽  
Content Type ◽  
Drug Candidates ◽  
New Class ◽  
Novel Targets ◽  
Essential Enzyme ◽  
Strain H37rv

ABSTRACTBenzothiazinones (BTZ) are a new class of drug candidates to combat tuberculosis that inhibit decaprenyl-phosphoribose epimerase (DprE1), an essential enzyme involved in arabinan biosynthesis. Using the checkerboard method and cell viability assays, we have studied the interaction profiles of BTZ043, the current lead compound, with several antituberculosis drugs or drug candidates againstMycobacterium tuberculosisstrain H37Rv, namely, rifampin, isoniazid, ethambutol, TMC207, PA-824, moxifloxacin, meropenem with or without clavulanate, and SQ-109. No antagonism was found between BTZ043 and the tested compounds, and most of the interactions were purely additive. Data from two different approaches clearly indicate that BTZ043 acts synergistically with TMC207, with a fractional inhibitory concentration index of 0.5. TMC207 at a quarter of the MIC (20 ng/ml) used in combination with BTZ043 (1/4 MIC, 0.375 ng/ml) had a stronger bactericidal effect onM. tuberculosisthan TMC207 alone at a concentration of 80 ng/ml. This synergy was not observed when the combination was tested on a BTZ-resistantM. tuberculosismutant, suggesting that DprE1 inhibition is the basis for the interaction. This finding excludes the possibility of synergy occurring through an off-target mechanism. We therefore hypothesize that sub-MICs of BTZ043 weaken the bacterial cell wall and allow improved penetration of TMC207 to its target. Synergy between two new antimycobacterial compounds, such as TMC207 and BTZ043, with novel targets, offers an attractive foundation for a new tuberculosis regimen.

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