The Use of Nanostructured Gibberellic Acid to Optimize the Cultivation of Echinacea purpurea (L.) Moench In Vitro

2020 ◽  
Vol 17 (9) ◽  
pp. 4718-4724
Author(s):  
Elena V. Maslova ◽  
Alexander A. Krolevets ◽  
Polina A. Gaidai ◽  
Elena A. Kalashnikova ◽  
Mikhail Y. Cherednichenko ◽  
...  
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Exposure Time ◽  
Effective Action ◽  
Active Substance ◽  
Callus Tissue ◽  
Echinacea Purpurea ◽  
Action Time ◽  
Sterilization Process

The sterilization process of plant explants of E. purpurea was optimized when introduced into an In Vitro culture, the most effective sterilizers, their exposure time and concentration were selected. An approach to increase the yield of plant explants seedlings from Echinacea purpurea (L.) Moench seeds is proposed. During the study an effective action time of 1 hour and a concentration of 1% nanocapsulated gibberellic acid were selected for more successful seed germination and cultivation of E. purpurea in vitro. This mode is at the same time the most economical both in terms of exposure time and in the minimum consumption of the active substance. The most optimal media compositions were determined, both for microclonal propagation and for the formation of E. purpurea callus tissue in in vitro culture.

scholarly journals Introduction of hyssopus officinalis l . into in vitro culture to optimize the conditions for obtaining callus tissues and microclonal propagation as a promising metod of innovative agrobiotechnologies

2021 ◽  
Vol 30 ◽  
pp. 05006
Author(s):  
Elena Maslova ◽  
Natalya Gulya ◽  
Tatyana Perelugina ◽  
Valeria Semykina ◽  
Elena Kalashnikova
Keyword(s):  
In Vitro Culture ◽  
Exposure Time ◽  
Crop Production ◽  
Callus Tissue ◽  
Nutrient Media ◽  
Sterilization Process ◽  
Callus Tissues ◽  
Active Substances ◽  
Hyssopus Officinalis

The sterilization process of plant explants of H. officinalis was optimized when introduced into an in vitro culture, the most effective sterilization modes, optimal sterilizing agents, their exposure time and concentration were selected. Callus tissues and mini-plants of H. officinalis were obtained in vitro and the most optimal nutrient media were determined both for microclonal propagation and for the induction of callus tissue H. officinalis, which can be further used for mass cultivation of cell and culture and obtaining safe bio-additives with active substances for livestock and crop production as a part of the development of modern agrobiotechnologies.

scholarly journals The Effect of Hormone Seed Priming Using Gibberellic Acid on Seed Germination Characteristics and Seedling Growth of Coneflower (Echinacea purpurea)

2021 ◽  
Vol 8 (1) ◽  
pp. 173-188
Author(s):  
Farshid Yousefi ◽  
Abdolreza Sihampoosh ◽  
Abdolmahdi Bakhshandeh ◽  
Seyyed Amir Mousavi ◽  
◽  
...  
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Seedling Growth ◽  
Seed Priming ◽  
Echinacea Purpurea ◽  
Germination Characteristics

In vitro Organogenesis in Cotton (Gossypium spp) for Ex-situ Conservation Issue

2021 ◽  
pp. 108-119
Author(s):  
Marius Guillaume Sinha ◽  
Gilles Habib Todjro Cacaï ◽  
Jerome Anani Houngue ◽  
Serge Sètondji Houédjissin ◽  
Thiéris Dassoudo Sossou ◽  
...  
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Activated Charcoal ◽  
Genetic Erosion ◽  
Ex Situ ◽  
Naphthalene Acetic Acid ◽  
Zygotic Embryos ◽  
Local Varieties ◽  
Regeneration Rate

Background: Monovarietal cultivation of cotton plant allowed the genetic erosion for traditional cotton varieties that proceed essential traits mainly involved in cotton genetic breeding. These varieties need to be preserved for future used. This study aims to evaluate the effect of gibberellic acid on cotton seed germination and the effect of Benzylaminopurin (BAP), Kinetin (KIN), α-naphthalene acetic acid (NAA) and activated charcoal (CA) on cotton seedlings growth obtained from different type of explants. Methodology: The seeds of three improved varieties (KET782, ANG956, OKP768) and five local varieties (Q62, Q64, Q85, Q88, Q92) were germinated with different concentration of gibberellic acid (GA3). Different explants were collected from the germinated plantlets and cultivated in different culture media containing plants growth regulators and activated charcoal in different concentration. Results: We found that Gibberellic Acid activity on cotton seeds germination significantly varied according to the variety and GA3 concentration in the medium. 63.33% germination rate in OKP768 was obtained on the medium containing 0.5 mg/L of GA3 while the medium with 1 mg/l of GA3 gave the highest seed germination in Q85 (75%), Q64 (69.17%), and ANG956 (40.83%). The plantlets regeneration rate varied within the explants in different varieties used. 100%of axillary nodes were regenerated in Q62; Q85 and Q92 varieties while 60% were regenerated with the zygotic embryos in ANG956, and OKP768. Moreover, the medium supplemented with GA3 (1mg/l) + NAA (1 mg/l) induced the greatest number of roots (2.75 roots/plantlet) in the ANG956 and OKP768 varieties. With activated charcoal (10 g/l), ANG956 and OKP768 varieties achieved better performance with respective roots length average of 3.4 cm/plantlet and 2.1 cm/plantlet. The activated charcoal at 10 g/l highly influenced the length of roots with an average of 7.7cm in ANG956 variety. Implication: The protocol established during this study will be useful for in vitro regeneration and conservation for cotton local varieties.

scholarly journals Effect of gibberellic acid, stratification and salinity on seed germination of Echinacea purpurea cv. Magnus

2015 ◽  
Vol 61 (3) ◽  
pp. 13-22 ◽  
Author(s):  
Shamila Yadolahi Zadeh ◽  
Ali Akbar Ramin ◽  
Bahram Baninasab
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Salinity Stress ◽  
Echinacea Purpurea ◽  
Purple Coneflower ◽  
Randomized Design ◽  
Salinity Levels ◽  
The Mean ◽  
Completely Randomized Design ◽  
Mean Time

SummaryThis study was conducted in order to determine the appropriate treatment for breaking dormancy and the effect of salinity on seed germination of purple coneflower (Echinacea purpureacv. Magnus), in two separate experiments. In the first experiment, five levels of gibberellic acid (GA3) (0, 250, 500, 1000, and 1500 mg×L−1) with four levels of cold moist stratification period of seeds at 5°C (0, 5, 10 and 15 days) were launched. A factorial experiment was conducted in a completely randomized design with four replications. The statistical analysis showed that concentration of 250 mg×L−1GA3with 10 days of cold moist chilling significantly increased the percentage of germination of normal seedlings and reduced the mean time of germination. In the second experiment, the seeds were chilled for 10 days at 5°C and half of them treated with 250 mg×L−1GA3for 24 hours. The seeds treated with GA3, and those non-treated were subjected to NaCl for salinity stress. The experiment was conducted using five salinity levels (0, 20, 40, 60 and 80 mM NaCl) in four replications in a completely randomized design. The results showed that purple coneflower is highly sensitive to salinity in the germination stage. The results also showed that by increasing salinity levels, the percentage of germination and normal seedlings significantly decreased and the mean time to germination increased, compared to the control treatment. But the seeds treated with GA3showed higher viability and better performance under salinity stress condition.

scholarly journals In vitro seed germination and seedling development of Annona crassiflora Mart.

2009 ◽  
Vol 66 (3) ◽  
pp. 410-413 ◽  
Author(s):  
Márcia de Nazaré Oliveira Ribeiro ◽  
Moacir Pasqual ◽  
Fabíola Villa ◽  
Leila Aparecida Salles Pio ◽  
Henk William Maria Hilhorst
Keyword(s):  
Acetic Acid ◽  
Seed Germination ◽  
Gibberellic Acid ◽  
Seedling Development ◽  
Interactive Effects ◽  
Naphthalene Acetic Acid ◽  
Feasible Alternative ◽  
Annona Crassiflora ◽  
Short Time

Annona crassiflora Mart known as 'araticum', 'marolo' or 'field araticum' is a typical fruit from the Cerrado biome of Brazil with socio-economic and medicinal importance. Normally, Annona crassiflora is propagated through seeds. However, due to a deep dormancy that the seeds display at dispersion and the difficulty to obtain uniform plants in a short time period, micropropagation may be a feasible alternative. Concentrations of gibberellic acid (GA3) and naphthalene-acetic acid (NAA) and their interactive effects on in vitro seed germination and seedling development of Annona crassiflora were studied. Mature fruits of Annona crassiflora were depulped and the seeds washed in clear water and dried at room temperature. Seed coat was removed and the seeds were placed on Murashige & Skoog (MS) medium supplemented with gibberellic acid (GA3) and naphthalene-acetic acid (NAA), 30 g L-1 sucrose and 6 g L-1 agar-agar. Seeds were kept under these conditions for 30 days. After this period, seedlings were kept for another 90 days on Wood Plant Medium (WPM) with 20 g L-1 sucrose and 5 g L-1 agar-agar supplemented with the same GA3 and NAA concentrations. Cultures were incubated under controlled conditions at 25 ± 2°C temperature, 16: 8 (light: dark) photoperiod of 32 µmol m-2 s-1 irradiance provided by cool white fluorescent tubes (Philips). Use of WPM medium supplemented with 25-32 mg L-1 GA3 or MS with 26-30 mg L-1 GA3 and 2 mg L-1 NAA promoted rooting and plant growth.

scholarly journals The Development of Gametophyte Sterilization Method for Liverworts Acrolejeunea fertilis (Reinw., Blume and Nees) Schiffn. In vitro Culture

2021 ◽  
Vol 28 (1) ◽  
pp. 39
Author(s):  
Mouleidi Dwi Putri ◽  
Windri Handayani ◽  
Astari Dwiranti ◽  
Andi Salamah ◽  
Niarsi Merry Hemelda ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Exposure Time ◽  
Common Type ◽  
Internal Contamination ◽  
Sterilization Method ◽  
Combination Treatments ◽  
Sterilization Process ◽  
Bacteria And Fungi ◽  
High Level

In vitro culture gametophytes of leafy liverworts often have problems in their sterilization process. These problems due to the high level of contamination and the fragile structure of the gametophyte leafy liverworts. The structures can be easily to damage after exposure to disinfectant. This study aimed to observe the concentration and the exposure time of “Bayclin” commercial bleach to suppress contamination with the viability of Acrolejeunea fertilisgametophytic explants. This research was conducted using control and 6 combination treatments with “Bayclin” concentration (1.00%, 1.25%, and 1.50%) and exposure time 60 and 120 seconds, then accompanied by the addition of Tetracycline 2.5 mg/ml. The qualitative parameters observed were the explant color, the type and location of contamination, and the growth of explants. The quantitative parameters were the percentage of contamination, the percentage of growth, and the number of new branches. The results showed that “Bayclin” 1.25% and 1.50% with 60 seconds exposure time has the lowest percentage of contamination which is 70% until the 7th days after planting. The most common type of internal contamination from the explant is bacteria and fungi. However, the growth of the new branch still occurs in some explants even though it has been contaminated and browned.

scholarly journals Dormancy and Germination In Vitro Response of Hydrangea macrophylla and Hydrangea paniculata Seed to Light, Cold-Treatment and Gibberellic Acid

2010 ◽  
Vol 28 (1) ◽  
pp. 41-47 ◽  
Author(s):  
Stephen P. Greer ◽  
Timothy A. Rinehart
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Red Light ◽  
Cold Treatment ◽  
Seed Viability ◽  
In Vitro Study ◽  
Potassium Nitrate ◽  
Hydrangea Macrophylla ◽  
Solid Media

Abstract Seed germination was optimized for ten Hydrangea macrophylla (Thunb.) Ser. and two Hydrangea paniculata Siebold cultivars in vitro. Methods were also developed to assay seed physiology. Best results for in vitro study were obtained with 0.5× Gamborgs solid media in conjunction with Plant Preservative Mixture (PPM), and by sterilizing seed with trichloro-s-triazinetrione (Trichlor). Assays of physiology were conducted by sterilizing seed and treating with combinations of white and red light, cold-treatment, gibberellic acid and potassium nitrate, and light cycles. Estimates of seed viability/dormancy, germination of non-dormant seed, and germination overall were calculated for each treatment combination. The most favorable conditions for overall Hydrangea seed germination were cold-treatment for 6 weeks, imbibition with GA3 + KNO3, and plating on half-strength Gamborgs media supplemented with GA3 in the presence of white light.

scholarly journals Promotion of Immature Seed Germination in Jacaranda mimosifolia

HortScience ◽  
2005 ◽  
Vol 40 (5) ◽  
pp. 1485-1486
Author(s):  
Ikuo Miyajima ◽  
Adriana Kato ◽  
Juan Carlos Hagiwara ◽  
Diego Mata ◽  
Gabriela Facciuto ◽  
...  
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Seedling Stage ◽  
Immature Seed ◽  
In Vitro Germination ◽  
Immature Seeds ◽  
Mature Seeds ◽  
Jacaranda Mimosifolia

In vitro germination of immature seeds of Jacaranda mimosifolia treated with gibberellic acid (GA3) was studied. Immature seeds were collected monthly after crossings and sown on Murashige and Skoog (1962) medium with 3.0% sucrose and 0.6% agar after soaked 24 hours with 0, 10, 100, and 500 mg·L–1 GA3 solutions. Though germination was observed in the immature seeds harvested 2 months after crossing (2 MAC), the rate was quite low. When immature seeds of 3 MAC treated with 100 or 500 mg·L–1 GA3 solution were cultured, >60% germination were obtained within 2 weeks after culturing. These results indicate that immature seeds of 3 MAC treated with adequate GA3 solutions, seedlings can be obtained precociously and the period from crossing to the seedling stage was shorter than for mature seeds.

scholarly journals The Development of In Vitro Culture Sterilization Method of Gametophyte Explant Lopholejeunea sp.

2021 ◽  
Vol 28 (2) ◽  
pp. 110
Author(s):  
Anna Widyastuti ◽  
Afiatry Putrika ◽  
Astari Dwiranti ◽  
Andi Salamah ◽  
Niarsi Merry Hemelda ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Exposure Time ◽  
Shoot Formation ◽  
Common Type ◽  
In Vitro Cultures ◽  
Sterilization Process ◽  
Bacteria And Fungi ◽  
High Level ◽  
Viable Culture

In vitro cultures of leafy liverworts are still facing significant challenges due to high-level of explant contamination. The sterilization process can easily damage the structure of liverwort after exposure to the disinfectant. This study was to determine the concentration and time exposure of commercial bleach as a disinfectant to suppress contamination using the gametophyte culture of Lopholejeunea sp. The experiment consisted of control and six treatment combinations of commercial bleach with concentration 0.5, 0.75, and 1% (v/v), and exposure time (60 and 90 seconds). The type and location of contamination, the color of the explants after sterilization, and response after 30 days were observed. The results showed that the 0.75% bleach with 60 and 90 seconds exposure time had a lower contamination until the 7th day of culture. The most common type of contamination is bacteria and fungi that arise from the explant. Despite the contamination, it did not inhibit shoot formation. Further studies still needed to determine the type of fungicides and antibiotics with the most potent concentration and exposure time should be tested to obtain an axenic and viable culture of liverworts Lopholejeunea sp.

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