Quantitative Bacteriology of Tonsils Removed from Children with Tonsillitis Hypertrophy and Recurrent Tonsillitis with and without Hypertrophy

1995 ◽  
Vol 104 (8) ◽  
pp. 646-652 ◽  
Author(s):  
Jeffrey J. Kuhn ◽  
L. W. Preston Church ◽  
Itzhak Brook ◽  
David A. Bianchi ◽  
Catherine L. Waters ◽  
...  
Keyword(s):  
Bacterial Species ◽  
Bacterial Load ◽  
Anaerobic Bacteria ◽  
Tonsillar Hypertrophy ◽  
Recurrent Tonsillitis ◽  
Eikenella Corrodens ◽  
Etiologic Role ◽  
Prevotella Melaninogenica ◽  
Aerobic And Anaerobic ◽  
Number Of Bacteria

The aerobic and anaerobic bacterial species and their numbers were studied in tonsillar specimens from children who had undergone elective tonsillectomy: 6 patients with recurrent tonsillitis (RT), 9 with recurrent tonsillitis with hypertrophy (RTH), and 8 with obstructive tonsillar hypertrophy (OTH). Mixed flora were present in all tonsils, yielding an average of 6.7 isolates (5.6 aerobic or facultative and 1.1 anaerobic bacteria). The highest recovery rate of organisms per tonsil was in patients with OTH (7.7 per tonsil), compared to 6.3 per tonsil in RT and 5.9 per tonsil in RTH. The predominant aerobic and facultative organisms were Haemophilus influenzae (22 isolates), Neisseria sp (16), Staphylococcus aureus (14), and Eikenella corrodens (14), and the predominant anaerobic bacteria were Fusobacterium sp (8), Bacteroides sp (7), and Prevotella melaninogenica (5). The number of bacteria per gram of tonsillar tissue varied between 104 and 108. A higher concentration of S aureus and H influenzae was found in hypertrophic tonsils (RTH and OTH) as compared to RT. These findings suggest the presence of an increased bacterial load and supports an etiologic role for H influenzae and S aureus in hypertrophic tonsils with and without inflammation (RTH and OTH). Further studies to elucidate the effect of selective antimicrobial therapy directed at these organisms may offer an alternative management of hypertrophic tonsils.

Immunoglobulin- and complement-coated bacteria in pus from peritonsillar abscesses

1998 ◽  
Vol 112 (7) ◽  
pp. 634-638 ◽  
Author(s):  
Markus Lilja ◽  
Simo Räisänen ◽  
Lars-Eric Stenfors
Keyword(s):  
Bacterial Species ◽  
Bacterial Flora ◽  
Anaerobic Bacteria ◽  
Inflammatory Cells ◽  
Immunofluorescence Assay ◽  
Peritonsillar Abscess ◽  
Secretory Iga ◽  
Intracellular Bacteria ◽  
Protective Mechanisms ◽  
Aerobic And Anaerobic

AbstractFifty-five samples of pus were collected from 51 acute, non-perforated, two spontaneously ruptured and two recurrent peritonsillar abscesses (35 males and 18 females; median age 18 years) and analysed regarding (i) aerobic and anaerobic bacteria (standard culturing), (ii) morphology of bacteria and inflammatory cells (direct microscopy of acridine orange-stained material), and (iii) the percentage of bacteria coated with immunoglobulins IgG, secretory IgA (SIgA) andIgM and complement cleavage product C3b (immunofluorescence assay). Seventy-one per cent of the abscesses harboured a mixed bacterial flora of various aerobes and anaerobes. In none of the cases with a single bacterial species (27 per cent) could immunoglobulin- or complement-coated bacteria be found. In abscesses with a mixed flora, 18 per cent harboured IgG-coated, 15 per cent SIgA-coated, five per cent IgM-coated and five per cent C3b-coated bacteria, respectively. All pus samples contained inflammatory cells in abundance but they were mostly deformed and only occasionally could intracellular bacteria be recognized. Insufficient immunoglobulin-coating of bacteria might be an important aetiopathogenic factor in the development of a peritonsillar abscess. Bactericide in the abscesses is accomplished chiefly by protective mechanisms not dependent on antigen recognition by antibodies.

Prevention of Bacteremia in Dogs Undergoing Dental Scaling by Prior Administration of Oral Clindamycin or Chlorhexidine Oral Rinse

2000 ◽  
Vol 17 (1) ◽  
pp. 11-16 ◽  
Author(s):  
Terry L. Bowersock ◽  
Ching Ching Wu ◽  
Gregory A. Inskeep ◽  
S. Ted Chester
Keyword(s):  
Anaerobic Bacteria ◽  
Automated System ◽  
Dental Scaling ◽  
Oral Rinse ◽  
Oral Regimen ◽  
Before And After ◽  
Prior Administration ◽  
Aerobic And Anaerobic ◽  
Number Of Bacteria

Dogs with periodontitis were used to determine the efficacy of an oral regimen of clindamycin versus chlorhexidine acetate oral rinse in reducing the total number of bacteria and the incidence of bacteremia before and after dental scaling. Aerobic and anaerobic bacteria, isolated from blood and gingival swab cultures, were identified to genus using an automated system.

Paronychia: a Mixed Infection

1993 ◽  
Vol 18 (3) ◽  
pp. 358-359 ◽  
Author(s):  
I. BROOK
Keyword(s):  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Klebsiella Pneumoniae ◽  
Mixed Infection ◽  
Anaerobic Bacteria ◽  
Eikenella Corrodens ◽  
Bacteroides Species ◽  
Group A ◽  
Aerobic And Anaerobic ◽  
Fusobacterium Species

Cultures of paronychia of the fingers have grown aerobic and anaerobic bacteria. Each was recovered individually in only about a quarter of the patients and a mixed aerobic/anaerobic flora was isolated in half of the patients. The predominant anaerobic organisms were Gram-positive anaerobic cocci, Bacteroides species and Fusobacterium species. The predominant aerobic organisms were Staphylococcus aureus, gamma-haemolytic streptococci, Eikenella corrodens, group A beta-hemolytic streptococci, alpha-hemolytic streptococci, and Klebsiella pneumoniae. Candida albicans was recovered in about 15% of the cases. The management of this infection is discussed.

scholarly journals Associations between Salivary Cytokines and Periodontal and Microbiological Parameters in Patients undergoing Fixed Orthodontic Appliance Treatment

2019 ◽  
Author(s):  
Yong Chen ◽  
Wong Wing Kit ◽  
Jayampath C Seneviratne ◽  
Shuying Huang ◽  
Colman McGrath ◽  
...  
Keyword(s):  
Health Status ◽  
Bacterial Load ◽  
Anaerobic Bacteria ◽  
Gingival Inflammation ◽  
Orthodontic Appliance ◽  
Orthodontic Therapy ◽  
Periodontal Inflammation ◽  
Gingival Health ◽  
Fixed Orthodontic Appliance ◽  
Aerobic And Anaerobic

Abstract Background: Orthodontic treatment can lead to microbial-induced gingival inflammation and aseptic periodontal inflammations. The aim of this study was to investigate the relationship between salivary pro-inflammatory cytokines levels with gingival health status and oral microbe loads among patients undergoing fixed orthodontic appliance treatment (FOAT). Methods: This was a cross-sectional study among a sample of 111 consecutive orthodontic patients (mean age 18.4 ± 4.4 years). Clinical examinations were conducted to assess the gingival health status employing the Modified Gingival Index (MGI), Gingival Bleeding Index (GBI) and Plaque Index (PI). Salivary microbiological assessments of total aerobic and anaerobic bacteria count, streptococci count and lactobacilli count were undertaken. Salivary immunological assessments included Interleukin-1Beta (IL-1β) and macrophage migration inhibitory factor (MIF) ELISA assays. Results: The mean ± standard deviation (SD) of salivary IL-1β was 83.52 ± 85.62 pg/ml and MIF was 4.12 ± 0.96 ng/ml. Positive significant correlations between salivary IL-1β levels and salivary bacteria counts (r=0.380~0.446, P<0.001), and between salivary MIF levels and total salivary aerobic and anaerobic bacteria counts (r=0.249~0.306, P<0.01) were observed. A significant positive correlation was found between salivary IL-1β levels and BI (r=0.216, p<0.05). Conclusions: The levels of salivary IL-1β and MIF correlate with oral bacterial load among patients undergoing fixed orthodontic therapy. This has implications in understanding gingival inflammation/ aseptic periodontal inflammation during orthodontic therapy.

Aerobic and Anaerobic Bacteria in Tonsils of Children with Recurrent Tonsillitis

1981 ◽  
Vol 90 (3) ◽  
pp. 261-263 ◽  
Author(s):  
Itzhak Brook ◽  
Paula Yocum ◽  
Ellen M. Friedman
Keyword(s):  
Anaerobic Bacteria ◽  
Core Material ◽  
Beta Lactamase ◽  
Type B ◽  
Recurrent Tonsillitis ◽  
Patients Âgés ◽  
Anaerobic Microorganisms ◽  
Group A ◽  
Group B ◽  
Aerobic And Anaerobic

Tonsils were obtained from 50 children suffering from recurrent tonsillitis. Patients' ages ranged from 2.5 to 17 years (mean 6 years); 29 were males and 21 females. The tonsils were sectioned in half after heat searing of the surface and the core material was cultured for aerobic and anaerobic microorganisms. Mixed aerobic and anaerobic flora was obtained in all patients, yielding an average of 7.8 isolates (4.1 anaerobes and 3.7 aerobes) per specimen. There were 207 anaerobes isolated. The predominant isolates were 101 Bacteroides sp (including 10 B fragilis group, and 47 B melaninogenicus group), 39 Fusobacterium sp, 34 Gram-positive anaerobic cocci (25 Peptococcus sp and 9 Peptostreptococcus sp) and 16 Veillonella sp. There were 185 aerobic isolates. The predominant isolates were 41 α-hemolytic streptococci, 24 Staphylococcus aureus, 19 β-hemolytic streptococci (11 group A, 4 group B, and 2 each group C and F), 14 Haemophilus sp (including 12 H influenzae type B) and 5 H parainfluenzae. Beta-lactamase production was noted in 56 isolates recovered from 37 tonsils. These were all isolates of S aureus (24) and B fragilis (10), 15 of 47 B melaninogenicus (32%), 5 of 12 B oralis (42%), and 2 of 12 H influenzae type B (17%). Our findings indicate the polymicrobial aerobic and anaerobic nature of deep tonsillar flora in children with recurrent tonsillitis, and demonstrate the presence of many β-lactamase-producing organisms in 74% of the patients.

Aerobic and Anaerobic Bacteria in Tonsils of Different Ages with Recurrent Tonsillitis

2019 ◽  
Vol 10 (9) ◽  
pp. 1022
Author(s):  
Gulbahar F. Karim ◽  
Siham Sh. AL-Salihi ◽  
Qanat Mahmood Atya ◽  
Kasim Sakran Abass
Keyword(s):  
Anaerobic Bacteria ◽  
Recurrent Tonsillitis ◽  
Different Ages ◽  
Aerobic And Anaerobic

scholarly journals Effectiveness of 0.66% Povidone-Iodine Eye Drops on Ocular Surface Flora before Cataract Surgery: A Nationwide Microbiological Study

2021 ◽  
Vol 10 (10) ◽  
pp. 2198
Author(s):  
Rosario Musumeci ◽  
Pasquale Troiano ◽  
Marianna Martinelli ◽  
Matteo Piovella ◽  
Claudio Carbonara ◽  
...  
Keyword(s):  
Cataract Surgery ◽  
Ocular Surface ◽  
Povidone Iodine ◽  
Bacterial Species ◽  
Bacterial Flora ◽  
Bacterial Load ◽  
Controlled Study ◽  
Eye Drops ◽  
Microbiological Analysis ◽  
Contralateral Eye

A multicenter, nonrandomized, prospective, controlled study was conducted to evaluate, as perioperative prophylactic treatment, the anti-infective effectiveness of 0.66% povidone-iodine eye drops (IODIM®) against the bacterial flora of the conjunctival surface of patients who undergo cataract surgery. Eye drops containing 0.66% povidone-iodine were applied to the eye undergoing cataract surgery; the untreated contralateral eye was used as control. One hundred and twenty patients set to receive unilateral cataract surgery were enrolled in 5 Italian Ophthalmology Centers and pretreated for three days with 0.66% povidone-iodine eye drops. The contralateral eye, used as control, was left untreated. Conjunctival swabs of both eyes were collected at the baseline visit and after three days of treatment, just before the cataract surgery. A qualitative and quantitative microbiological analysis of bacterial presence was evaluated by means of bacterial culture, followed by identification. Methicillin resistance determination was also performed on staphylococci isolates. Bacterial load before and after treatment of the eye candidate for cataract surgery was evaluated and compared to the untreated eye. A reduction or no regrowth on the culture media of the bacterial load was observed in 100% of the study subjects. A great heterogenicity of bacterial species was found. The 0.66% povidone-iodine eye drops, used for three days prior to cataract surgery, were effective in reducing the conjunctival bacterial load. The 0.66% povidone-iodine eye drops (IODIM®) might represent a valid perioperative prophylactic antiseptic adjuvant treatment to protect the ocular surface from microbial contamination in preparation of the surgical procedure.

scholarly journals Volatile scents of influenza A and S. pyogenes (co-)infected cells

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Selina Traxler ◽  
Gina Barkowsky ◽  
Radost Saß ◽  
Ann-Christin Klemenz ◽  
Nadja Patenge ◽  
...  
Keyword(s):  
Influenza A ◽  
Early Recognition ◽  
Bacterial Species ◽  
Bacterial Load ◽  
Gas Chromatography Mass Spectrometry ◽  
Sampling System ◽  
Infected Cells ◽  
Infection Processes ◽  
Non Destructive

AbstractInfluenza A is a serious pathogen itself, but often leads to dangerous co-infections in combination with bacterial species such as Streptococcus pyogenes. In comparison to classical biochemical methods, analysis of volatile organic compounds (VOCs) in headspace above cultures can enable destruction free monitoring of metabolic processes in vitro. Thus, volatile biomarkers emitted from biological cell cultures and pathogens could serve for monitoring of infection processes in vitro. In this study we analysed VOCs from headspace above (co)-infected human cells by using a customized sampling system. For investigating the influenza A mono-infection and the viral-bacterial co-infection in vitro, we analysed VOCs from Detroit cells inoculated with influenza A virus and S. pyogenes by means of needle-trap micro-extraction (NTME) and gas chromatography mass spectrometry (GC-MS). Besides the determination of microbiological data such as cell count, cytokines, virus load and bacterial load, emissions from cell medium, uninfected cells and bacteria mono-infected cells were analysed. Significant differences in emitted VOC concentrations were identified between non-infected and infected cells. After inoculation with S. pyogenes, bacterial infection was mirrored by increased emissions of acetaldehyde and propanal. N-propyl acetate was linked to viral infection. Non-destructive monitoring of infections by means of VOC analysis may open a new window for infection research and clinical applications. VOC analysis could enable early recognition of pathogen presence and in-depth understanding of their etiopathology.

Aerobic and Anaerobic Bacteria in the Middle ear and ear Canal in Acute Otitis Media

1982 ◽  
Vol 93 (sup386) ◽  
pp. 100-102 ◽  
Author(s):  
J. Luotonen ◽  
A. M. M. Jokipii ◽  
P. Sipilä ◽  
J. Väyrynen ◽  
L. Jokipii ◽  
...  
Keyword(s):  
Otitis Media ◽  
Middle Ear ◽  
Anaerobic Bacteria ◽  
Acute Otitis Media ◽  
Ear Canal ◽  
Aerobic And Anaerobic

scholarly journals Spatial and Temporal Features of the Growth of a Bacterial Species Colonizing the Zebrafish Gut

mBio ◽  
2014 ◽  
Vol 5 (6) ◽  
Author(s):  
Matthew Jemielita ◽  
Michael J. Taormina ◽  
Adam R. Burns ◽  
Jennifer S. Hampton ◽  
Annah S. Rolig ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Spatial Structure ◽  
Growth Kinetics ◽  
Spatial Organization ◽  
Temporal Dynamics ◽  
Bacterial Species ◽  
Bacterial Load ◽  
Light Sheet ◽  
Logistic Growth ◽  
Light Sheet Fluorescence Microscopy

ABSTRACTThe vertebrate intestine is home to microbial ecosystems that play key roles in host development and health. Little is known about the spatial and temporal dynamics of these microbial communities, limiting our understanding of fundamental properties, such as their mechanisms of growth, propagation, and persistence. To address this, we inoculated initially germ-free zebrafish larvae with fluorescently labeled strains of anAeromonasspecies, representing an abundant genus in the zebrafish gut. Using light sheet fluorescence microscopy to obtain three-dimensional images spanning the gut, we quantified the entire bacterial load, as founding populations grew from tens to tens of thousands of cells over several hours. The data yield the first ever measurements of the growth kinetics of a microbial species inside a live vertebrate intestine and show dynamics that robustly fit a logistic growth model. Intriguingly, bacteria were nonuniformly distributed throughout the gut, and bacterial aggregates showed considerably higher growth rates than did discrete individuals. The form of aggregate growth indicates intrinsically higher division rates for clustered bacteria, rather than surface-mediated agglomeration onto clusters. Thus, the spatial organization of gut bacteria both relative to the host and to each other impacts overall growth kinetics, suggesting that spatial characterizations will be an important input to predictive models of host-associated microbial community assembly.IMPORTANCEOur intestines are home to vast numbers of microbes that influence many aspects of health and disease. Though we now know a great deal about the constituents of the gut microbiota, we understand very little about their spatial structure and temporal dynamics in humans or in any animal: how microbial populations establish themselves, grow, fluctuate, and persist. To address this, we made use of a model organism, the zebrafish, and a new optical imaging technique, light sheet fluorescence microscopy, to visualize for the first time the colonization of a live, vertebrate gut by specific bacteria with sufficient resolution to quantify the population over a range from a few individuals to tens of thousands of bacterial cells. Our results provide unprecedented measures of bacterial growth kinetics and also show the influence of spatial structure on bacterial populations, which can be revealed only by direct imaging.

Sign in / Sign up

Export Citation Format

Share Document