Biological Activity of Serum Prolactin in Patients with Primary Hypothyroidism

Prolactin bioactivity was measured in sera from 22 patients with primary hypothyroidism and 13 euthyroid control subjects and compared with estimates of immunoactivity given by radioimmunoassay. The Nb2 rat lymphoma cell assay was modified to improve specificity for prolactin and used to measure bioactivity. Results of serum prolactin levels obtained using the bioassay were closely correlated with radioimmunoassay results in both hypothyroid patients and control subjects. Mean bioactivity/immunoactivity ratios in patients were not significantly different from those in control subjects. Mean prolactin concentration measured by both assays was significantly higher in patients than control subjects. Serum prolactin in patients with primary hypothyroidism appears to have essentially normal bioactivity as measured in the Nb2 assay, in contrast with a report of major differences between activity in radioreceptor assay and RIA in hypothyroid patients.

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Serum prolactin in uraemia: correlations between bioactivity and activity in two immunoassays

Acta Endocrinologica ◽

10.1530/acta.0.1200295 ◽

1989 ◽

Vol 120 (3) ◽

pp. 295-300 ◽

Cited By ~ 5

Author(s):

Caroline R. Smith ◽

Joan Butler ◽

Neil Iggo ◽

Michael R. Norman

Keyword(s):

Biological Activity ◽

Peritoneal Dialysis ◽

Clinical Significance ◽

Elevated Serum ◽

Biologically Active ◽

Serum Prolactin ◽

Immunoradiometric Assay ◽

Control Subjects ◽

Significant Difference ◽

And Control

Abstract. The clinical significance of hyperprolactinaemia in uraemic patients is uncertain and discrepancies between immunoactivity and biological activity of serum hPRL have been reported. We have modified the Nb2 cell bioassay to improve specificity for hPRL and used this assay to measure hPRL bioactivity in sera from 26 uraemic patients and 40 control subjects. Seventeen patients were receiving regular haemodialysis and 9 continuous ambulatory peritoneal dialysis. Levels of hPRL bioactivity were compared with hPRL immunoactivity measured by RIA (PRL-RIA) and by immunoradiometric assay (PRL-IRMA). Serum hPRL levels measured by all three assays were significantly elevated in uraemic patients compared with control subjects (P< 0.001). The immunoradiometric method gave significantly lower results than RIA in control subjects but not in uraemic patients (P< 0.05). There was no significant difference in mean ratio of hPRL bioactivity to PRL-RIA between patients and control subjects (1.18 ± 0.05 vs 1.11 ± 0.03, mean ± sem). The ratio of hPRL bioactivity to PRL-IRMA was slightly decreased in uraemic patients compared with controls (P = 0.05). Serum hPRL bioactivity was closely correlated with immunoactivity in both immunoassays (r ≥ 0.96) in patients and controls. These results confirm that elevated serum hPRL levels in uraemic patients represent biologically active hormone which may contribute to hypogonadism.

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Serum prolactin levels in sons of alcoholics and control subjects

American Journal of Psychiatry ◽

10.1176/ajp.144.7.854 ◽

1987 ◽

Vol 144 (7) ◽

pp. 854-859 ◽

Cited By ~ 65

Keyword(s):

Serum Prolactin ◽

Control Subjects ◽

And Control

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The Effects of a Neutral Stimulus (Buzzer) on Motor Responses and Disfluencies in Normal Speakers

Journal of Speech and Hearing Research ◽

10.1044/jshr.1201.179 ◽

1969 ◽

Vol 12 (1) ◽

pp. 179-184 ◽

Cited By ~ 7

Author(s):

Richard R. Martin ◽

Gerald M. Siegel

Keyword(s):

College Students ◽

Neutral Stimulus ◽

Motor Responses ◽

Control Subjects ◽

And Control ◽

Speaking Task

Seventy-two college students were divided into three groups: Button Push-Speech (BP-S), Speech-Button Push (S-BP), and Control. BP-S subjects pushed one of two buttons on signal for 8 min. During the last 4 min, depression of the criterion button caused a buzzer to sound. After the button-push task, subjects spoke spontaneously for 30 min. During the last 20 min, the buzzer was presented contingent upon each disfluency. S-BP subjects were run under the same procedures, but the order of button-push and speech tasks was reversed. Control subjects followed the same procedures as S-BP subjects, but no buzzer signal was presented at any time. Both S-BP and BP-S subjects emitted significantly fewer disfluencies during the last 20 min (Conditioning) than during the first 10 min (Baserate) of the speaking task. The frequency of disfluencies for Control subjects did not change significantly from Baserate to Conditioning. In none of the three groups did the frequency of pushes on the criterion button change significantly from minute to minute throughout the 8-min button-push session.

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Tests for Platelet Changes, Acute Phase Reactants and Serum Lipids in Diabetes mellitus and Peripheral Vascular Disease

Thrombosis and Haemostasis ◽

10.1055/s-0038-1657283 ◽

1982 ◽

Vol 48 (03) ◽

pp. 289-293 ◽

Cited By ~ 17

Author(s):

B A van Oost ◽

B F E Veldhuyzen ◽

H C van Houwelingen ◽

A P M Timmermans ◽

J J Sixma

Keyword(s):

Diabetes Mellitus ◽

Vascular Disease ◽

Peripheral Vascular Disease ◽

Acute Phase ◽

Serum Lipids ◽

Diabetic Patients ◽

Peripheral Vascular ◽

Acute Phase Reactants ◽

Control Subjects ◽

And Control

SummaryPlatelets tests, acute phase reactants and serum lipids were measured in patients with diabetes mellitus and patients with peripheral vascular disease. Patients frequently had abnormal platelet tests and significantly increased acute phase reactants and serum lipids, compared to young healthy control subjects. These differences were compared with multidiscriminant analysis. Patients could be separated in part from the control subjects with variables derived from the measurement of acute phase proteins and serum lipids. Platelet test results improved the separation between diabetics and control subjects, but not between patients with peripheral vascular disease and control subjects. Diabetic patients with severe retinopathy frequently had evidence of platelet activation. They also had increased acute phase reactants and serum lipids compared to diabetics with absent or nonproliferative retinopathy. In patients with peripheral vascular disease, only the fibrinogen concentration was related to the degree of vessel damage by arteriography.

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The fate of orally administered sialic acid: First insights from patients with N-acetylneuraminic acid synthase deficiency and control subjects

Molecular Genetics and Metabolism Reports ◽

10.1016/j.ymgmr.2021.100777 ◽

2021 ◽

Vol 28 ◽

pp. 100777

Author(s):

Christel Tran ◽

Licia Turolla ◽

Diana Ballhausen ◽

Sandrine Cornaz Buros ◽

Tony Teav ◽

...

Keyword(s):

Sialic Acid ◽

Acetylneuraminic Acid ◽

Control Subjects ◽

And Control

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Cognitive Function and Mortality in a Community-based Elderly Cohort of First-ever Stroke Survivors and Control Subjects

Journal of Stroke and Cerebrovascular Diseases ◽

10.1016/j.jstrokecerebrovasdis.2009.07.006 ◽

2010 ◽

Vol 19 (5) ◽

pp. 382-387 ◽

Cited By ~ 12

Author(s):

Peter Hobson ◽

Jolyon Meara

Keyword(s):

Cognitive Function ◽

Stroke Survivors ◽

Community Based ◽

Control Subjects ◽

Elderly Cohort ◽

And Control

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Mandibular deviations in TMD and non-TMD groups related to eye dominance and head posture

Journal of Clinical Pediatric Dentistry ◽

10.17796/jcpd.25.2.j7171238p2413611 ◽

2002 ◽

Vol 25 (2) ◽

pp. 147-155 ◽

Cited By ~ 12

Author(s):

N. Pradham ◽

G. White ◽

N. Mehta ◽

A. Forgione

Keyword(s):

Head Rotation ◽

Lateral Direction ◽

Head Posture ◽

Control Subjects ◽

Eye Dominance ◽

Pain Center ◽

And Control ◽

Female Subjects

This study was designed to determine whether eye-dominance affects head posture (rotation) and in turn, whether head posture is associated with mandibular frenum midline deviation, in both TMJ and control subjects. Eye dominance was determined using three tests:Porta, Hole, Point tests. Natural head posture was evaluated using the Arthrodial protractor. Mandibular frenum deviation was recorded as left, right or no deviation. Fifty female subjects were included in the study, 25 TMJ patients attending the Gelb Craniomandibular Pain Center and 25 non-TMJ control subjects. The findings indicate that eye dominance and direction of head rotation are strongly associated in both TMJ and control subjects. Further, in TMJ subjects mandibular deviation occurred in greater frequency than in controls and tends to occur in the contra lateral direction of head rotation.

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