scholarly journals Quality Assessment of Urinary Organic Acid Analysis

1994 ◽  
Vol 31 (2) ◽  
pp. 129-133 ◽  
Author(s):  
J R Bonham ◽  
M Downing ◽  
R J Pollitt ◽  
N J Manning ◽  
K H Carpenter ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Organic Aciduria ◽  
Gas Chromatography Mass Spectrometry ◽  
Average Score ◽  
Tyrosinaemia Type ◽  
Maple Syrup ◽  
Significant Difference ◽  
Chain Acyl ◽  
Urinary Organic Acid Analysis ◽  
The Uk

The number of known inherited metabolic disorders resulting in an organic aciduria has increased steadily over the past two decades. Prompt and reliable detection is both clinically and technically demanding but is essential if appropriate treatment is to be undertaken. This is the first study of laboratory performance in the detection of these disorders to be undertaken in the UK. Some conditions were accurately identified by most laboratories: for example for maple syrup urine disease, 12 of 14 laboratories provided an appropriate response and medium chain acyl-CoA dehydrogenase deficiency was correctly identified by 15 of 17 laboratories. However, accuracy of detection was poorer for other conditions: for example, only eight of 17 laboratories detected tyrosinaemia type 1 and nine of 18 laboratories detected 4-hydroxybutyric aciduria. The strongest correlation with good performance was obtained by comparison with the extent of peak identification: r=0·62, P=0·002. The need for regular attendance at scientific symposia was also supported by a weaker positive correlation with the average score achieved, P=0·08. Evidence also suggested that some of the laboratories with a low workload performed less well. No significant difference in performance could be demonstrated between the 17 laboratories who used gas chromatography -mass spectrometry and the six participants who used gas chromatography alone.

scholarly journals Determination of ethanol in micro-volumes of blood by headspace gas chromatography: Statistical comparison between capillary and venous sampling sites

2020 ◽  
pp. 002580242092863
Author(s):  
Luke Taylor ◽  
Vytautas Remeškevičius ◽  
Lili Saskoy ◽  
Tara Brodie ◽  
Jeshan Mahmud ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Sample Preparation ◽  
Venous Blood ◽  
Forensic Toxicology ◽  
Capillary Blood ◽  
Blood Alcohol ◽  
Venous Sampling ◽  
Preparation Methods ◽  
Significant Difference ◽  
The Uk

Ethanol is the most commonly encountered drug in forensic toxicology, with widespread use throughout society. For this reason, it is important that there are a variety of reliable and robust methods to detect and quantify the content of alcohol in blood samples of suspected drink drivers. A common method of detection is gas chromatography with flame ionisation detector, with a number of sample preparation techniques employed. Typically, venous blood is sampled and used in the analysis. However, there is currently no legal specification in the UK of the blood sample source. This study investigates the use of capillary blood as an alternative to venous blood alongside two different sample volumes: 100 and 10 µL. Venous and capillary blood were collected from volunteers who had consumed alcohol. All blood sampling was carried out one hour after cessation of drinking. The results show a statistically significant difference between venous and capillary samples, with an average difference of 3.38 ± 1.99 mg/100 mL at 100 μL and approximately 4.13 ± 2.42 mg/100 mL at 10 μL, respectively. Predominantly, venous blood was detected at higher concentrations than the corresponding capillary samples. The deviations in alcohol samples between venous and capillary blood are consistent with previous studies. However, our research indicates that capillary blood is a viable matrix to test for alcohol, albeit one that underestimates blood-alcohol content in relation to venous sampling. There was no statistically significant difference between the 100 and 10 µL sample preparation methods on an individual basis, which infers that micro-volumes of alcohol are suitable for forensic blood-alcohol analysis.

scholarly journals Constituents and Anthelmintic Activity Evaluation of Albizia Adiantifolia (Schumach) W.F. Wright Essential Oils FromNigeria

2018 ◽  
Vol 10 (2) ◽  
pp. 10 ◽  
Author(s):  
Akinsola Akande ◽  
Sherifat Aboaba ◽  
Guido Flamini
Keyword(s):  
Gas Chromatography ◽  
Essential Oils ◽  
Anthelmintic Activity ◽  
Stem Bark ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Root Bark ◽  
Dependent Manner ◽  
Caryophyllene Oxide ◽  
Significant Difference

Albizia adiantifolia (Schumach) W.F. Wright (Fabaceae) is a plant used traditionally in treating different health ailments which includes worm infections. The essential oils (EOs) were obtained by hydrodistillation in an all glass Clevenger apparatus, and characterized by gas chromatography (GC) and gas chromatography-mass spectrometry analysis (GC-MS). In vitro petri-dish anthelmintic activity was carried out using adult earthworm, Eudrilus eugeniae. The leaves, stem bark and root bark EOs afforded a total of 40, 26 and 20 constituents representing 90.9%, 94.1% and 90.9% of the total oil fractions respectively. The classes of compounds identified in the leaves, stem bark and root bark are oxygenated monoterpenes (4.1%, 1.7% and 4.0% respectively), sesquiterpene hydrocarbons (39.5%, 67.3% and 42.6% respectively), oxygenated sesquiterpenes (18.7%, 22.3% and 30.1% respectively), non-terpene derivatives (12.1%, 2.6% and 14.2% respectively) and apocarotenoids (16.5% and 0.2% in the leaves and stem-bark). β-caryophyllene (23.0%), E-geranylacetone (7.4%), acorenone (6.4%), viridiflorol (6.4%), α-zingiberene (6.3%) and ar-curcumene (4.6%) were the major constituents in the leaves oil, β-caryophyllene (39.3%), selin-11-en-4-α-ol (10.4%), α-zingiberene (9.6%), ar-curcumene (7.2%), caryophyllene oxide (6.4%) and α-humulene (5.6%) were the major constituents in the stem bark oil, while β-caryophyllene (32.1%), selin-11-en-4-α-ol (13.1%), caryophyllene oxide (8.4%), pentadecanal (6.1%) and α -humulene (4.4%) were the major constituents in the root bark oil. β -caryophyllene dominated the oils. The leaf EO was the most active against E. eugeniae worm. All the oils showed a relatively higher activity compared to Albendazole, in a concentration dependent manner. There was significant difference (p<0.001) in activity.

Simpler liquid-chromatographic screening for organic acid disorders.

1984 ◽  
Vol 30 (4) ◽  
pp. 542-546 ◽  
Author(s):  
M J Bennett ◽  
C E Bradey
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Gas Chromatography ◽  
Volatile Fatty Acids ◽  
Simultaneous Detection ◽  
Organic Aciduria ◽  
Gas Chromatography Mass Spectrometry ◽  
Identification System ◽  
Chromatographic Technique ◽  
Normal Infant

Abstract We describe an isocratic cation-exchange chromatographic technique, with ultraviolet detection, for determination of urinary organic acids. The method is useful for screening urines from neonates and infants suspected of having an organic aciduria, prior to confirmatory analysis by gas chromatography-mass spectrometry. Advantages of this technique over conventional gas chromatography include ease of sample preparation and the simultaneous detection of volatile fatty acids; limitations include its lack of an identification system like that of mass spectrometry, the presence of nonultraviolet-absorbing fatty acids, and the relatively long retention times of phenolic compounds. Chromatograms for patients with methylmalonic aciduria, propionic aciduria, isovaleric aciduria, beta-ketothiolase deficiency, lactic aciduria, and glutaric aciduria type II are compared with those for a normal infant.

scholarly journals A Study on the Volatile Compounds in Elaeagnus angustifolia L. Flowers during Flowering Season by Gas Chromatography-Mass Spectrometry Coupled with Advanced Chemometrics

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Zhijing Liu ◽  
Degang Ding ◽  
Huapeng Cui ◽  
Chuanchuan Wang ◽  
Juntao Liu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Volatile Compounds ◽  
Gas Chromatography Mass Spectrometry ◽  
Analysis Tool ◽  
Elaeagnus Angustifolia ◽  
Full Bloom ◽  
Chromatography Mass Spectrometry ◽  
Flowering Season ◽  
Significant Difference

The flowers of Elaeagnus angustifolia L. have been used as a homologous variety in China, whose quality seriously relies on the compositions during the flowering period. Unfortunately, studies on the variations of volatile compounds during the flowering season are rarely reported. Herein, a gas chromatography-mass spectrometry-based untargeted metabolomic methodology was proposed for the comprehensive analysis of volatile compounds in E. angustifolia flowers to classify various flowering stages. Samples from four flowering stages were collected, including the initial bloom stage, pre-full bloom stage (70–80% of flowers), full bloom stage, and ending of the bloom stage. Simultaneous distillation extraction was used for the extraction of volatile compounds in the flowers, which was then analyzed by a newly developed chemometric data analysis tool, autoGCMSDataAnal. An advantage of the developed methodology is that compounds can be accurately screened and identified. Finally, 59 compounds that showed significant difference among four flowering stages were screened and 31 compounds were identified. Sample clustering results from principal component analysis and hierarchical clustering analysis suggested that flowers from the pre-full bloom stage and full bloom stage may be more suitable when used as raw materials for industrial products.

scholarly journals Yield and composition of lavender essential oil grown in substrate

2018 ◽  
Vol 36 (2) ◽  
pp. 259-264
Author(s):  
Ana PS Mambrí ◽  
Jerônimo L Andriolo ◽  
Melânia P Manfron ◽  
Suany MG Pinheiro ◽  
Francieli L Cardoso ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Essential Oil ◽  
Biomass Yield ◽  
Dry Mass ◽  
Planting Date ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Composition ◽  
Significant Difference ◽  
Made In

ABSTRACT The objective of this research was to evaluate the effect of successive harvesting times and shading on the production of biomass, yield and phytochemical composition of essential oil in leaves and inflorescences of soilless grown Lavandula dentata. Plants were grown in pots filled with sand, inside a polyethylene greenhouse, in a closed system at UFSM. Plants were harvested at 150, 213 and 320 days after planting date (DAP) in winter, spring and summer, respectively. At 150 DAP, a 50% shading screen was installed. The experimental design was a randomized 3x2 factorial in subdivided plots with 36 plants per subplot. Fresh (FM) and dry mass (DM), yield and chemical composition of essential oil in leaves and inflorescences were determined after each harvest date. The essential oil was extracted, using 70 g fresh mass of leaves and of inflorescences, respectively. Identification and quantification of compounds in the essential oil were determined by gas chromatography/mass spectrometry (GC/MS). The FM of inflorescences was higher on plants harvested 213 DAP grown without shading while of leaves it was higher on plants harvested 320 DAP grown without shading. The yield of the essential oil was higher in the leaves of plants harvested 320 DAP grown without shading. Thirty-one compounds were identified and quantified, being the major ones 1.8 cineol, camphor and linalool, without significant difference among treatments. Three consecutive harvests can be made in August (150 DAP), October (213 DAP) and February (320 DAP) without replacing plants.

scholarly journals Analysis of 5-hydroxytryptamine and its related indoles in cerebrospinal fluid of leukemic children by gas chromatography-mass spectrometry

2020 ◽  
Vol 44 (1) ◽  
pp. 41-45
Author(s):  
Pingping Zhang ◽  
Bo Wang ◽  
Yanmei Sun ◽  
Jian Gao ◽  
Kaoqi Lian
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Cerebrospinal Fluid ◽  
Acetic Acid ◽  
Reliable Method ◽  
Lymphoblastic Leukemia ◽  
Gas Chromatography Mass Spectrometry ◽  
Good Sensitivity ◽  
Chromatography Mass Spectrometry ◽  
Significant Difference

Abstract Background It is meaningful to quantify some neurotransmitters in cerebrospinal fluid (CSF) for pathology and clinical diagnosis. The objective of this study was to develop a rapid and reliable method for detecting 5-hydroxyindole ethanol (5-HTOL), 5-hydroxyindole acetic acid (5-HIAA), 5-hydroxytryptophan (5-HTP) and 5-hydroxytryptamine (5-HT) in CSF by gas chromatography-mass spectrometry (GC-MS), and explore the clinical significance of the levels of these neurotransmitters in CSF from acute lymphoblastic leukemia (ALL). Methods The levels of 5-HTOL, 5-HIAA, 5-HTP and 5-HT in CSF from children with ALL and a control (CON) group were examined by the proposed GC-MS method. Results The GC-MS method showed good sensitivity and accuracy. 5-HT and 5-HIAA contents in the ALL group were significantly lower than those in the CON group (p < 0.01), while there was no significant difference in 5-HTP and 5-HTOL contents between the two groups (p > 0.05). Conclusions This sensitive and reliable method could be used in studies focusing on clinical pathogenesis of ALL.

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