scholarly journals Determination of ethanol in micro-volumes of blood by headspace gas chromatography: Statistical comparison between capillary and venous sampling sites

2020 ◽  
pp. 002580242092863
Author(s):  
Luke Taylor ◽  
Vytautas Remeškevičius ◽  
Lili Saskoy ◽  
Tara Brodie ◽  
Jeshan Mahmud ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Sample Preparation ◽  
Venous Blood ◽  
Forensic Toxicology ◽  
Capillary Blood ◽  
Blood Alcohol ◽  
Venous Sampling ◽  
Preparation Methods ◽  
Significant Difference ◽  
The Uk

Ethanol is the most commonly encountered drug in forensic toxicology, with widespread use throughout society. For this reason, it is important that there are a variety of reliable and robust methods to detect and quantify the content of alcohol in blood samples of suspected drink drivers. A common method of detection is gas chromatography with flame ionisation detector, with a number of sample preparation techniques employed. Typically, venous blood is sampled and used in the analysis. However, there is currently no legal specification in the UK of the blood sample source. This study investigates the use of capillary blood as an alternative to venous blood alongside two different sample volumes: 100 and 10 µL. Venous and capillary blood were collected from volunteers who had consumed alcohol. All blood sampling was carried out one hour after cessation of drinking. The results show a statistically significant difference between venous and capillary samples, with an average difference of 3.38 ± 1.99 mg/100 mL at 100 μL and approximately 4.13 ± 2.42 mg/100 mL at 10 μL, respectively. Predominantly, venous blood was detected at higher concentrations than the corresponding capillary samples. The deviations in alcohol samples between venous and capillary blood are consistent with previous studies. However, our research indicates that capillary blood is a viable matrix to test for alcohol, albeit one that underestimates blood-alcohol content in relation to venous sampling. There was no statistically significant difference between the 100 and 10 µL sample preparation methods on an individual basis, which infers that micro-volumes of alcohol are suitable for forensic blood-alcohol analysis.

Effects of Different Methods Used to Take Blood Samples on Blood Glucose Measurements

2021 ◽  
pp. 105477382110247
Author(s):  
Eda Ergin ◽  
Ayten Zaybak
Keyword(s):  
Blood Glucose ◽  
Glucose Tolerance Test ◽  
Venous Blood ◽  
Tolerance Test ◽  
Capillary Blood ◽  
Oral Glucose ◽  
Blood Samples ◽  
Significant Difference ◽  
Measurement Results ◽  
Quasi Experimental

The purpose of this study is to compare whether or not there is a difference between venous and capillary blood samples in blood glucose measurements and investigate the effects of different aseptic methods used in skin cleaning before collecting blood samples on measurement results. This quasi-experimental study was conducted with 109 patients. The capillary first and second blood drop values taken from the patients after fasting and at 2 hours following 75 g oral glucose tolerance test (OGTT) and capillary and venous blood glucose values were compared. There was no significant difference between the median venous blood glucose value and the capillary second blood drop value taken after wiping the finger with alcohol. There was no significant difference between the first and second blood drop values of capillary blood glucose 2 hours after OGTT.

The prevalence of anaemia among Saudi school girls: a comparison of two techniques

2016 ◽  
Vol 13 (2) ◽  
pp. 82-88
Author(s):  
Elham Abbas Aljaaly ◽  
Nahlaa Abduwahab Khalifa
Keyword(s):  
Venous Blood ◽  
National Sample ◽  
Capillary Blood ◽  
Primary Data ◽  
Content Type ◽  
Jeddah City ◽  
System Method ◽  
Significant Difference ◽  
High Prevalence ◽  
School Girls

Purpose – Continuous integration of health screens to investigate and track variations in the students’ healthiness over time is essential. However, the sustainability of this practice remains challenging. The purpose of this paper is to identify the prevalence of anaemia based on haemoglobin (Hb) estimation among Saudi school girls in Jeddah city by examining capillary and venous blood. Design/methodology/approach – The design is investigative, using primary data to define the prevalence of anaemia based on two techniques. Samples of venous (n=408) and capillary (n=797) blood from Saudi school girls in Jeddah city was collected and compared using: Reflotron Plus System method (capillary blood) and ABX MICROS 60-OT Automated Haematology Analyser for (venous blood). Findings – When the capillary blood was used, the prevalence of anaemia was 40 per cent (n=319 out of 797). Using the venous blood, the prevalence was 29.4 per cent (n=120 out of 408). Comparison of Hb data for both tests showed a significant difference in Hb levels with levels between the 10g and 12 g/dl particularly and both measurements demonstrated good agreement (ICC=0.87, 95 per cent CI (0.845, 0.892)). Research limitations/implications – The data were collected only in Jeddah city. Further studies should include a national sample that is representative of both gender from different backgrounds and geographical areas in the kingdom of Saudi Arabia. Practical implications – The high prevalence of anaemia within the school girls’ population require decision makers to devise a set of maintainable projects to progress students’ general health, including health education and supplementation such as iron, particularly if anaemia is diagnosed to be nutrition-related. This particularly applied to the health centres at female schools that have anaemia screening programmes that going on in Saudi schools. Originality/value – This study reveals a technique-based measures of anaemia prevalence that have public and practical health implications.

scholarly journals Analytical performance of lateral flow immunoassay for SARS-CoV-2 exposure screening on venous and capillary blood samples

2020 ◽  
Author(s):  
Margaret A Black ◽  
Guomiao Shen ◽  
Xiaojun Feng ◽  
Wilfredo Garcia Beltran ◽  
Yang Feng ◽  
...  
Keyword(s):  
Whole Blood ◽  
Colloidal Gold ◽  
Venous Blood ◽  
Capillary Blood ◽  
Lateral Flow ◽  
Antibody Detection ◽  
Lateral Flow Immunoassay ◽  
Blood Samples ◽  
Significant Difference ◽  
Finger Stick

Objectives: Numerous serologic immunoassays have been launched to detect antibodies to SARS-CoV-2, including rapid tests. Here, we validate use of a lateral flow immunoassay (LFI) intended for rapid screening and qualitative detection of anti-SARS-CoV-2 IgM and IgG in serum, plasma, and whole blood, and compare results with ELISA. We also seek to establish the value of LFI testing on blood obtained from a capillary blood sample. Methods: Samples collected by venous blood draw and capillary finger stick were obtained from patients with SARS-CoV-2 detected by RT-qPCR and control patients negative for SARS-CoV-2. Samples were tested with the 2019-nCoV IgG/IgM Detection Kit (Colloidal Gold) lateral flow immunoassay, and antibody calls were compared with results obtained by ELISA. Results: The Biolidics LFI kit shows clinical sensitivity of 92% at 7 days after PCR diagnosis of SARS-CoV-2 on venous blood. Test specificity was 92% for IgM and 100% for IgG. There was no significant difference in detecting IgM and IgG with Biolidics LFI and ELISA at D0 and D7 (p=1.00), except for detection of IgM at D7 (p=0.04). Finger stick whole blood of SARS-CoV-2 patients showed 93% sensitivity for antibody detection. Conclusions: Clinical performance of Biolidics 2019-nCoV IgG/IgM Detection Kit (Colloidal Gold) is comparable to ELISA and showed consistent results across different sample types. Furthermore, we show that capillary blood obtained by finger stick shows similar sensitivity for detecting anti-SARS-CoV-2 IgM and IgG antibodies as venous blood samples. This provides an opportunity for decentralized rapid testing in the community and may allow point-of-care and longitudinal self-testing for the presence of anti-SARS-CoV-2 antibodies.

scholarly journals The Correlation of Ig M Anti PGL-1 antibody between blood veins and dryed capillary blood on filter papers in household contact of leprosy patient

2019 ◽  
Author(s):  
Anisha Calista Prakoeswa ◽  
Bayu Bijaksana Rumondor ◽  
Meva Nareza Trianita ◽  
I. Iswahyudi ◽  
Fatma Rosida ◽  
...  
Keyword(s):  
Filter Paper ◽  
Large Scale ◽  
Venous Blood ◽  
Household Contact ◽  
Capillary Blood ◽  
Serological Screening ◽  
Significant Difference ◽  
Leprosy Patients ◽  
The Mean ◽  
Filter Papers

Delays of leprosy detection and treatment can lead to disability and potential transmission. Serologic examination has the advantage in detecting Subclinical Leprosy. The procedure of serologic test, which is one of its main limitation, could be simplified by the use filter paper. This study aims to assess the effectiveness of the use of capillary blood dropped on filter paper as a substitute for venous blood in household contact of leprosy patients. Seventeen samples of capillary blood dried on filter paper and venous blood samples from the same individual were examined by ELISA method to determine the levels of IgM anti-Phenolic glycolipid-1 (PGL-1). The mean of anti- PGL-1 IgM levels of filter paper samples 163.31±126.16; whereas the mean of levels from venous samples was 473.16±411.26. There was significant difference and correlation between these two groups. Samples on filter paper in household contact can be used to determine the level of anti-PGL-1 IgM in serum by converting on the regression basis. Further study is required to evaluate the potency of filter paper methods to conduct large-scale serological screening.

A Comparison of Venous versus Capillary Blood Samples when Measuring Blood Glucose Using a Point-of-Care, Capillary-Based Glucometer

2019 ◽  
Vol 34 (05) ◽  
pp. 506-509
Author(s):  
Jessica Topping ◽  
Matthew Reardon ◽  
Jake Coleman ◽  
Brian Hunter ◽  
Haruka Shojima-Perera ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Point Of Care ◽  
Research Question ◽  
Venous Blood ◽  
Hospital Setting ◽  
Capillary Blood ◽  
Needle Stick Injury ◽  
Blood Samples ◽  
Significant Difference ◽  
Venous Sample

AbstractBackground:Blood glucose level (BGL) is routinely assessed by paramedics in the out-of-hospital setting. Most commonly, BGL is measured using a blood sample of capillary origin analyzed by a hand-held, point-of-care glucometer. In some clinical circumstances, the capillary sample may be replaced by blood of venous origin. Given most point-of-care glucometers are engineered to analyze capillary blood samples, the use of venous blood instead of capillary may lead to inaccurate or misleading measurements.Hypothesis/Problem:The aim of this prospective study was to compare mean difference in BGL between venous and capillary blood from healthy volunteers when measured using a capillary-based, hand-held, point-of-care glucometer.Methods:Using a prospective observational comparison design, 36 healthy participants provided paired samples of blood, one venous and the other capillary, taken near simultaneously. The BGL values were similar between the two groups. The capillary group had a range of 4.3mmol/l, with the lowest value being 4.4mmol/l and 8.7mmol/l the highest. The venous group had a range of 2.7mmol/l, with the lowest value being 4.1mmol/l and 7.0mmol/l the highest.For the primary research question, the mean BGL for the venous sample group was 5.3mmol/l (SD = 0.6), compared to 5.6mmol/l (SD = 0.8) for the capillary group. This represented a statistically significant difference of 0.3mmol/l (P = .04), but it did not reach the a priori established point of clinical significance (1.0mmol/l). Pearson’s correlation coefficient for capillary versus venous indicated moderate correlation (r = 0.42).Conclusion:In healthy, non-fasted people in a non-clinical setting, a statistically significant, but not clinically significant, difference was found between venous- and capillary-derived BGL when measured using a point-of-care, capillary-based glucometer. Correlation between the two was moderate. In this context, using venous samples in a capillary-based glucometer is reasonable providing the venous sample can be gathered without exposure of the clinician to risk of needle-stick injury. In clinical settings where physiological derangement or acute illness is present, capillary sampling would remain the optimal approach.

Reliability of Point-of-Care Capillary Blood Glucose Measurements in the Critical Value Range

2014 ◽  
Vol 138 (7) ◽  
pp. 962-966 ◽  
Author(s):  
Ron B. Schifman ◽  
Tan T. Nguyen ◽  
Susan T. Page
Keyword(s):  
Point Of Care ◽  
Venous Blood ◽  
Capillary Blood ◽  
Convenience Sample ◽  
Critical Range ◽  
Significant Difference ◽  
Patients With Diabetes ◽  
Value Range ◽  
Blood Specimens ◽  
One Year

Context.—Point-of-care glucose (POCG) testing on capillary blood specimens is central to maintaining glycemic control in patients with diabetes. Although there are known performance issues with POCG methods, especially for maintaining tight glucose control, there is little information about the accuracy of results in the critical ranges that may involve life-threatening conditions. Objectives.—To evaluate the reliability of POCG measurements in critical, high (>600 mg/dL) and low (<40 mg/dL) ranges. Design.—One-year retrospective analysis of POCG (ACCU-CHEK glucose meter, Roche Diagnostics Corporation, Indianapolis, Indiana) results for routine patient care were obtained. The frequency and accuracy of repeat testing after critical POCG results was analyzed. A convenience sample of noncritical capillary POCG measurements retested on venous blood specimens by another point-of-care device (RAPIDPoint 405 analyzer, Siemens Medical Solutions USA, Malvern, Pennsylvania) was also evaluated. Results.—Critical values were observed in 2.4 per 1000 POCG tests (256 of 105,928; 0.24%), with the highest rate (76 of 2289; 3.32%) from the emergency department. Twice as many critical high values as critical low values were seen. Nearly 80% of critical POCG tests (204 of 256) were repeated within 10 minutes. Of these 204 repeat measurements, 112 (54.9%) met accuracy criteria (±15 mg/dL of low and ±20% of high initial values). Accuracy was significantly higher when retesting was performed on the same meter or by the same operator (P ≤ .05). Comparison of capillary and venous POCG testing of noncritical results showed no significant difference (P = .95), with 89.8% (125 of 139) meeting accuracy criteria. Conclusions.—POCG measurements in the critical range are frequently erroneous, which is likely caused by preanalytic factors associated with sampling capillary blood. POCG testing practices should include retesting to confirm critical results.

scholarly journals Perbedaan Hitung Jumlah Trombosit Menggunakan Darah Vena dan Darah Kapiler

2016 ◽  
Vol 3 (2) ◽  
pp. 81
Author(s):  
Hieronymus Rayi Prasetya ◽  
Maria Irena Dentri ◽  
Sistiyono Sistiyono
Keyword(s):  
Platelet Count ◽  
Blood Platelets ◽  
Venous Blood ◽  
Blood Platelet ◽  
Capillary Blood ◽  
Blood Capillaries ◽  
Blood Samples ◽  
Platelet Counts ◽  
Significant Difference ◽  
Blood Platelet Count

Background: Platelets play a role in hemostasis which is the body's mechanisms to prevent and stop the bleeding. Platelets participate in the effort to close the wound, so that the body does not experience a loss of blood and protected from foreign cells. Examination of the platelet count is very important in the diagnosis of diseases, one of which is the diagnosis of dengue hemorrhagic fever (DHF). Examination of blood counts, especially platelets in clinical laboratories causes blood samples in use are not always the venous blood but could use capillary blood. Capillary blood samples are used primarily in pediatric patients, because the venous blood sampling is difficult, patient loads, and also shorten the time when taking blood. The purpose of this study was to determine whether there is a difference in counting the number of platelets using samples of blood veins and capillaries. Methods: Quantitative research with observational approach using a cross sectional study design in the 30 samples of student D3 Health Analyst STIKes To Nation Yogyakarta. Statistical methods in use are independent T test. Results: The research subjects were 30 samples of student D3 Health Analyst STIKes To Nation Yogyakarta. The results of the examination of venous blood platelet count and blood capillaries have different average values ​​are 247 530 cells / ml of blood, for blood platelets veins and 184 270 cells / ml of blood for capillary blood platelets. Spearman correlation analysis Obtained results of the examination of venous blood platelet count and blood capillaries normal distribution (p> 0.05). 0.129 venous blood platelet counts, while the number of blood platelets kapilernya 0.089. Conclusion: There is a significant difference from the results of counting the number of blood platelets using veins and capillaries, where the use of capillary blood samples showed that lower platelet counts.

scholarly journals Quality Assessment of Urinary Organic Acid Analysis

1994 ◽  
Vol 31 (2) ◽  
pp. 129-133 ◽  
Author(s):  
J R Bonham ◽  
M Downing ◽  
R J Pollitt ◽  
N J Manning ◽  
K H Carpenter ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Organic Aciduria ◽  
Gas Chromatography Mass Spectrometry ◽  
Average Score ◽  
Tyrosinaemia Type ◽  
Maple Syrup ◽  
Significant Difference ◽  
Chain Acyl ◽  
Urinary Organic Acid Analysis ◽  
The Uk

The number of known inherited metabolic disorders resulting in an organic aciduria has increased steadily over the past two decades. Prompt and reliable detection is both clinically and technically demanding but is essential if appropriate treatment is to be undertaken. This is the first study of laboratory performance in the detection of these disorders to be undertaken in the UK. Some conditions were accurately identified by most laboratories: for example for maple syrup urine disease, 12 of 14 laboratories provided an appropriate response and medium chain acyl-CoA dehydrogenase deficiency was correctly identified by 15 of 17 laboratories. However, accuracy of detection was poorer for other conditions: for example, only eight of 17 laboratories detected tyrosinaemia type 1 and nine of 18 laboratories detected 4-hydroxybutyric aciduria. The strongest correlation with good performance was obtained by comparison with the extent of peak identification: r=0·62, P=0·002. The need for regular attendance at scientific symposia was also supported by a weaker positive correlation with the average score achieved, P=0·08. Evidence also suggested that some of the laboratories with a low workload performed less well. No significant difference in performance could be demonstrated between the 17 laboratories who used gas chromatography -mass spectrometry and the six participants who used gas chromatography alone.

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