scholarly journals The Oral Microbiota Is Modified by Systemic Diseases

2018 ◽  
Vol 98 (2) ◽  
pp. 148-156 ◽  
Author(s):  
D.T. Graves ◽  
J.D. Corrêa ◽  
T.A. Silva
Keyword(s):  
Lupus Erythematosus ◽  
Leukocyte Adhesion ◽  
Periodontal Diseases ◽  
Oral Microbiome ◽  
Tooth Surface ◽  
Oral Microbiota ◽  
Systemic Diseases ◽  
Microbial Dysbiosis ◽  
Inflammatory Drugs ◽  
Periodontal Bone Loss

Periodontal diseases are initiated by bacteria that accumulate in a biofilm on the tooth surface and affect the adjacent periodontal tissue. Systemic diseases such as diabetes, rheumatoid arthritis (RA), and systemic lupus erythematosus (SLE) increase susceptibility to destructive periodontal diseases. In human studies and in animal models, these diseases have been shown to enhance inflammation in the periodontium and increase the risk or severity of periodontitis. All 3 systemic diseases are linked to a decrease in bacterial taxa associated with health and an increase in taxa associated with disease. Although there is controversy regarding the specific oral bacterial changes associated with each disease, it has been reported that diabetes increases the levels of Capnocytophaga, Porphyromonas, and Pseudomonas, while Prevotella and Selenomonas are increased in RA and Selenomonas, Leptotrichia, and Prevotella in SLE. In an animal model, diabetes increased the pathogenicity of the oral microbiome, as shown by increased inflammation, osteoclastogenesis, and periodontal bone loss when transferred to normal germ-free hosts. Moreover, in diabetic animals, the increased pathogenicity could be substantially reversed by inhibition of IL-17, indicating that host inflammation altered the microbial pathogenicity. Increased IL-17 has also been shown in SLE, RA, and leukocyte adhesion deficiency and may contribute to oral microbial changes in these diseases. Successful RA treatment with anti-inflammatory drugs partially reverses the oral microbial dysbiosis. Together, these data demonstrate that systemic diseases characterized by enhanced inflammation disturb the oral microbiota and point to IL-17 as key mediator in this process.

scholarly journals Oral microbial dysbiosis and its performance in predicting oral cancer

2020 ◽  
Author(s):  
Shih-Chi Su ◽  
Lun-Ching Chang ◽  
Hsien-Da Huang ◽  
Chih-Yu Peng ◽  
Chun-Yi Chuang ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Fusobacterium Nucleatum ◽  
Risky Behaviors ◽  
Health Condition ◽  
Oral Microbiome ◽  
Oral Microbiota ◽  
Microbial Dysbiosis ◽  
Anti Cancer ◽  
Betel Quid Chewing ◽  
Male Patients

Abstract Dysbiosis of oral microbiome may dictate the progression of oral squamous cell carcinoma (OSCC). Yet, the composition of oral microbiome fluctuates by saliva and distinct sites of oral cavity and is affected by risky behaviors (smoking, drinking and betel quid chewing) and individuals’ oral health condition. To characterize the disturbances in the oral microbial population mainly due to oral tumorigenicity, we profiled the bacteria within the surface of OSCC lesion and its contralateral normal tissue from discovery (n = 74) and validation (n = 42) cohorts of male patients with cancers of the buccal mucosa. Significant alterations in the bacterial diversity and relative abundance of specific oral microbiota (most profoundly, an enrichment for genus Fusobacterium and the loss of genus Streptococcus in the tumor sites) were identified. Functional prediction of oral microbiome shown that microbial genes related to the metabolism of terpenoids and polyketides were differentially enriched between the control and tumor groups, indicating a functional role of oral microbiome in formulating a tumor microenvironment via attenuated biosynthesis of secondary metabolites with anti-cancer effects. Furthermore, the vast majority of microbial signatures detected in the discovery cohort was generalized well to the independent validation cohort, and the clinical validity of these OSCC-associated microbes was observed and successfully replicated. Overall, our analyses reveal signatures (a profusion of Fusobacterium nucleatum CTI-2 and a decrease in Streptococcus pneumoniae) and functions (decreased production of tumor-suppressive metabolites) of oral microbiota related to oral cancer.

scholarly journals Analysis of Oral Microbiota Revealed High Abundance of Prevotella Intermedia in Gout Patients

2018 ◽  
Vol 49 (5) ◽  
pp. 1804-1812 ◽  
Author(s):  
Juan Liu ◽  
Li Cui ◽  
Xinmin Yan ◽  
Xinyuan Zhao ◽  
Jinluo Cheng ◽  
...  
Keyword(s):  
Serratia Marcescens ◽  
Oral Microbiome ◽  
Oral Microbiota ◽  
Prevotella Intermedia ◽  
Systemic Diseases ◽  
16S Sequencing ◽  
Control Subjects ◽  
Qpcr Analysis ◽  
Healthy Control ◽  
First Time

Background/Aims: Microbes reside in a number of body sites, including the oral cavity, and are associated with the progression of many systemic diseases. In this study, we aimed to investigate the effects of gout and hyperuricemia (HUA) on the composition of oral microbiomes. Methods: Analysis of the oral microbiota from 12 gout patients, 11 HUA patients, and 19 healthy control subjects was performed using a deep sequencing approach, and validation of significant changes in Prevotella intermedia and Serratia marcescens in new patient cohorts was performed using quantitative PCR (qPCR). Results: Our analysis indicated that both gout and HUA significantly altered the composition of the oral microbiome in patients. Patients with gout or HUA had significantly greater levels of salivary Prevotella intermedia but significantly lower levels of Serratia marcescens than healthy control subjects. Conclusion: We demonstrated the association between the oral microbiome and gout and HUA for the first time. In particular, 16S sequencing and qPCR analysis revealed significantly higher levels of oral Prevotella intermedia in gout/HUA patients, which suggests that these patients might be at risk for the development of periodontitis.

scholarly journals The Oral Microbiota: A Literature Review for Updating Professionals in Dentistry. Part I

2019 ◽  
pp. 143-152
Author(s):  
Cristina Barboza-Solís DDS, MSc, PhD ◽  
Luis Alberto Acuña-Amador PhD
Keyword(s):  
Oral Health ◽  
Scientific Evidence ◽  
Oral Microbiome ◽  
Current Evidence ◽  
Future Research ◽  
Oral Microbiota ◽  
Systemic Diseases ◽  
Conceptual Background ◽  
Keyword Searches

In recent decades, a body of literature examining the relationships between oral health and general health has rapidly developed. However, the biological mechanisms involved in explaining such relationships have not been fully described. Recent evidence has suggested that these relationships could be partially explained by the composition and interaction of the microbiome/microbiota between local and systemic body sites. For instance, it has been suggested that intestinal microbiota could have effects on non-communicable diseases, such as diabetes or cardiovascular diseases. The objective of this study is to explore current evidence of the link between oral and systemic diseases, to discuss whether oral microbiome/microbiota could represent an unexplored biological pathway partially explaining those relationships. A non-systematic review of the literature was carried out using keyword searches in Pubmed from February to May 2019. The ultimate goal was to present recent scientific evidence to update the general knowledge on this topic to professionals in dentistry. This review is divided in two parts for journal publication; however, it is intended to be used as one piece. In this first part, we will summarize the conceptual background of oral microbiome/microbiota, we will describe the main methods used in microbiology to characterize oral organisms, and will present the main composition of bacteria in oral microbiome/microbiota. The second part highlights the main evidence regarding the biological plausibility that links oral microbiome and systemic diseases and we will conclude with some future research recommendations. Taking into account the role of oral microbiota in the development of systemic diseases could change the main paradigm of how oral health is currently conceptualized by dental professionals.

scholarly journals Exploring the Oral Microbiome in Rheumatic Diseases, State of Art and Future Prospective in Personalized Medicine with an AI Approach

2021 ◽  
Vol 11 (7) ◽  
pp. 625
Author(s):  
Silvia Bellando-Randone ◽  
Edda Russo ◽  
Vincenzo Venerito ◽  
Marco Matucci-Cerinic ◽  
Florenzo Iannone ◽  
...  
Keyword(s):  
Personalized Medicine ◽  
Rheumatic Diseases ◽  
Lupus Erythematosus ◽  
Scientific Community ◽  
Oral Microbiome ◽  
Oral Microbiota ◽  
Systemic Lupus ◽  
Diagnostic Biomarkers ◽  
General Application ◽  
State Of Art

The oral microbiome is receiving growing interest from the scientific community, as the mouth is the gateway for numerous potential etiopathogenetic factors in different diseases. In addition, the progression of niches from the mouth to the gut, defined as “oral–gut microbiome axis”, affects several pathologies, as rheumatic diseases. Notably, rheumatic disorders (RDs) are conditions causing chronic, often intermittent pain affecting the joints or connective tissue. In this review, we examine evidence which supports a role for the oral microbiome in the etiology and progression of various RDs, including rheumatoid arthritis (RA), Sjogren’s syndrome (SS), and systemic lupus erythematosus (SLE). In addition, we address the most recent studies endorsing the oral microbiome as promising diagnostic biomarkers for RDs. Lastly, we introduce the concepts of artificial intelligence (AI), in particular, machine learning (ML) and their general application for understanding the link between oral microbiota and rheumatic diseases, speculating the application of a possible AI approach-based that can be applied to personalized medicine in the future.

scholarly journals Metagenomic and Metatranscriptomic Insight Into Oral Biofilms in Periodontitis and Related Systemic Diseases

2021 ◽  
Vol 12 ◽  
Author(s):  
Yi Huang ◽  
Xinyuan Zhao ◽  
Li Cui ◽  
Shaohong Huang
Keyword(s):  
Microbial Communities ◽  
Oral Microbiome ◽  
Etiologic Factor ◽  
Systemic Diseases ◽  
Pathogenic Microorganisms ◽  
Microbial Dysbiosis ◽  
Composition And Structure ◽  
Oral Biofilms ◽  
Systemic Health ◽  
Insight Into

The oral microbiome is one of the most complex microbial communities in the human body and is closely related to oral and systemic health. Dental plaque biofilms are the primary etiologic factor of periodontitis, which is a common chronic oral infectious disease. The interdependencies that exist among the resident microbiota constituents in dental biofilms and the interaction between pathogenic microorganisms and the host lead to the occurrence and progression of periodontitis. Therefore, accurately and comprehensively detecting periodontal organisms and dissecting their corresponding functional activity characteristics are crucial for revealing periodontitis pathogenesis. With the development of metagenomics and metatranscriptomics, the composition and structure of microbial communities as well as the overall functional characteristics of the flora can be fully profiled and revealed. In this review, we will critically examine the currently available metagenomic and metatranscriptomic evidence to bridge the gap between microbial dysbiosis and periodontitis and related systemic diseases.

scholarly journals Understanding the microbial components of periodontal diseases and periodontal treatment-induced microbiological shifts

2020 ◽  
Author(s):  
Ioannis Fragkioudakis ◽  
Marcello P. Riggio ◽  
Danae Anastasia Apatzidou
Keyword(s):  
Periodontal Diseases ◽  
Bacterial Species ◽  
Oral Microbiome ◽  
Periodontal Pocket ◽  
Oral Microbiota ◽  
Periodontal Pathogens ◽  
Susceptible Host ◽  
Modern Culture ◽  
Microbial Identification ◽  
Dental Biofilm

In the mid-1960s the microbial aetiology of periodontal diseases was introduced based on classical experimental gingivitis studies . Since then, numerous studies have addressed the fundamental role that oral microbiota plays in the initiation and progression of periodontal diseases. Recent advances in laboratory identification techniques have contributed to a better understanding of the complexity of the oral microbiome in both health and disease. Modern culture-independent methods such as human oral microbial identification microarray and next-generation sequencing have been used to identify a wide variety of microbial taxa residing in the gingival sulcus and the periodontal pocket. The first theory of the ‘non-specific plaque’ hypothesis gave rise to the ‘ecological plaque’ hypothesis and more recently to the ‘polymicrobial synergy and dysbiosis hypothesis’. Periodontitis is now considered to be a multimicrobial inflammatory disease in which the various bacterial species within the dental biofilm are in a dysbiotic state and this imbalance favours the establishment of chronic inflammatory conditions and ultimately the destruction of tooth-supporting tissues. Apart from the known putative periodontal pathogens, the whole biofilm community is now considered to play a role in the establishment of inflammation and the initiation and progression of periodontitis in a susceptible host. Treatment is unlikely to eliminate putative pathogens but, when it is thoroughly performed it has the potential to establish a healthy ecosystem by altering the microbial community in numbers and composition and also contribute to the maturation of the host immune response.

Oral Microbiome Signatures in Diabetes Mellitus and Periodontal Disease

2020 ◽  
Vol 99 (6) ◽  
pp. 658-665 ◽  
Author(s):  
T.E. Matsha ◽  
Y. Prince ◽  
S. Davids ◽  
U. Chikte ◽  
R.T. Erasmus ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Periodontal Disease ◽  
Periodontal Diseases ◽  
Conditional Logistic Regression ◽  
Oral Microbiome ◽  
Oral Microbiota ◽  
Pocket Depth ◽  
Logistic Regression Models ◽  
Gingival Bleeding ◽  
Rdna Sequencing

Disturbances in the oral microbiome are associated with periodontal disease initiation and progression and diabetes mellitus (DM), but how this contributes to the cause-and-effect relationship between periodontal disease and DM is poorly understood. We examined the bacterial composition in plaque samples from 128 South Africans with periodontal disease across glycemic statuses using 16S rDNA sequencing of regions 2, 3, 4, 6-7, 8, and 9. Of the 9 phyla identified, Firmicutes, Proteobacteria, Bacteroidetes, Fusobacteria, and Actinobacteria made up >98%. Fusobacteria and Actinobacteria were significantly more abundant in subjects with diabetes, while Proteobacteria were less abundant. However, in the presence of gingival bleeding and DM, as compared with DM without gingival bleeding, Actinobacteria were markedly reduced while Bacteroidetes were more abundant. In contrast, no differences in Actinobacteria or Bacteroidetes abundance were observed between DM with and without pocket depth (PD) ≥4 mm. At the genus level, similar changes in relative abundance were observed in the presence of DM and periodontal disease. Our findings remained in conditional logistic regression models adjusted for age, sex, waist circumference, and the 5 most dominant phyla. For example, Actinobacteria significantly increased the odds of diabetes by 10% in subjects with gingival bleeding, while Fusobacteria increased this odd by 14%; yet, among subjects with PD ≥4 mm, Fusobacteria decreased the odds of DM by 47%. Our findings have confirmed the alterations in the composition of the oral microbiota across glycemic statuses as well as different stages of periodontal disease. However, it is not clear whether these differences were the consequence of hyperglycemia or the presence of periodontal diseases. Therefore, we recommend further investigations in a longitudinal study design.

scholarly journals Oral Microbiota: A Major Player in the Diagnosis of Systemic Diseases

Diagnostics ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1376
Author(s):  
Charlotte Thomas ◽  
Matthieu Minty ◽  
Alexia Vinel ◽  
Thibault Canceill ◽  
Pascale Loubières ◽  
...  
Keyword(s):  
Periodontal Diseases ◽  
Systemic Disease ◽  
Oral Microbiota ◽  
Systemic Diseases ◽  
Oral Infections ◽  
New Therapeutic Approaches ◽  
Major Player ◽  
Health And Disease ◽  
Novel Target ◽  
Systemic Health

The oral cavity is host to a complex and diverse microbiota community which plays an important role in health and disease. Major oral infections, i.e., caries and periodontal diseases, are both responsible for and induced by oral microbiota dysbiosis. This dysbiosis is known to have an impact on other chronic systemic diseases, whether triggering or aggravating them, making the oral microbiota a novel target in diagnosing, following, and treating systemic diseases. In this review, we summarize the major roles that oral microbiota can play in systemic disease development and aggravation and also how novel tools can help investigate this complex ecosystem. Finally, we describe new therapeutic approaches based on oral bacterial recolonization or host modulation therapies. Collaboration in diagnosis and treatment between oral specialists and general health specialists is of key importance in bridging oral and systemic health and disease and improving patients' wellbeing.

Root Surface Characteristics of Children Teeth with Periodontal Diseases

2007 ◽  
Vol 32 (2) ◽  
pp. 101-104 ◽  
Author(s):  
E Bimstein ◽  
W Wignall ◽  
D Cohen ◽  
J Katz
Keyword(s):  
Down Syndrome ◽  
Light Microscopy ◽  
Leukocyte Adhesion ◽  
Periodontal Diseases ◽  
Surface Characteristics ◽  
Aggressive Periodontitis ◽  
Root Surface ◽  
Systemic Diseases ◽  
Leukocyte Adhesion Deficiency ◽  
Root Surfaces

The present study examined the root surfaces of teeth from children with or without periodontal diseases and with or without systemic diseases. Light microscopy revealed that when compared to control teeth: teeth with chronic periodontitis had similar radicular histology; teeth from children with leukocyte adhesion deficiency,Down syndrome and aggressive periodontitis had narrower cementum areas; teeth from children with hypophosphatasia showed cementum aplasia. Cementum anomalies may facilitate the establishment and progress of periodontitis in children.

The role of probiotics in correction of microbiocenosis and cytokine balance of the oral cavity of patient with chronic inflammatory disease of periodont

2020 ◽  
Vol 25 (4) ◽  
pp. 323-330
Author(s):  
E. S. Ovcharenko ◽  
V. V. Erichev ◽  
S. I. Risovannij ◽  
T. V. Aksenova ◽  
S. V. Melekhov ◽  
...  
Keyword(s):  
Oral Cavity ◽  
Periodontal Diseases ◽  
Treatment Protocol ◽  
Chronic Inflammatory Disease ◽  
Oral Microbiome ◽  
Comprehensive Treatment ◽  
Local Immunity ◽  
Treatment Techniques ◽  
Before And After

Relevance. A long-term inflammation in the oral cavity, unreasonable treatment of periodontal patients with antibiotics cause imbalance between certain types of fungal-bacterial associations accompanied by oral dysbiosis and change of local immune status. So, development and application of new comprehensive diagnosis and treatment techniques in periodontal patients, use of products for correction of microbiota and local immunity are a current and a long-term task. Purpose is to optimize the comprehensive treatment protocol of inflammatory periodontal diseases by introducing probiotics and evaluation of oral microbiome and cytokine profile.Materials and methods. 140 patients were examined. Of these 60 patients had chronic generalized plaque-induced gingivitis and 60 patients suffered from moderate chronic generalized periodontitis. Bacterial and fungal microbiome was assessed and the host immune response was evaluated in all patients before and after the treatment. Half of the patients were treated conventionally and the other half were treated according to a modified scheme: probiotic “Bifidumbacterin Forte” was added.Results. A large number of yeast-like fungi Candida and commensal bacteria were detected in periodontal pockets of patients with chronic generalized plaque-induced gingivitis and moderate chronic generalized periodontitis. That correlates with a significant increase of pro-inflammatory cytokines (TNF-α, IL—8), decrease of concentration of INF-γ and increase of antiinflammatory cytokine IL-4.Conclusion. Changes in clinical, microbiological and immunological values during a modified combination therapy with a probiotic and during a conventional treatment demonstrated that effectiveness of treatment of chronic gingivitis and chronic periodontitis increased by 40% and 37% respectively.

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