Preparation of three-dimensional multilayer ECM-simulated woven/knitted fabric composite scaffolds for potential tissue engineering applications

2019 ◽  
Vol 90 (7-8) ◽  
pp. 925-936 ◽  
Author(s):  
Dandan Guo ◽  
Shuai Wang ◽  
Yuxiang Yin ◽  
Jun Luo ◽  
Chenjie Meng ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Pore Diameter ◽  
Fiber Diameter ◽  
Three Dimensional ◽  
Single Layer ◽  
Woven Fabrics ◽  
Collagen Solution ◽  
Tendon Tissue Engineering ◽  
Bonding Properties ◽  
Composite Fabrics

The extracellular matrix (ECM), with its multilayer fiber structure, regulates diverse functions including cell proliferation, migration, differentiation and tissue regeneration effects. To mimic and replace the native ECM, the structures and properties of three single-layer fabric substrates including warp/weft-knitted and woven fabrics were analyzed, then two-layer warp/weft-knitted composite fabrics prepared by polyurethane (PU) bonding, and woven composite fabrics prepared by polycaprolactone (PCL)/collagen solution bonding or PU bonding, were studied. After PCL/collagen solution bonding or PU bonding, properties such as pore diameter, air permeability, stress and the contact angle of composite fabrics decreased by some degree, while fiber diameter, thickness and the thermal conductivity of composite fabrics increased. In combination with fiber diameter, pore diameter and physical properties, we know that warp- or weft-knitted composite fabrics are ideal scaffolda for potential applications in nerve, myocardium and tendon tissue engineering.

Three-Dimensional-Construct Bioreactor Conditioning in Human Tendon Tissue Engineering

2011 ◽  
Vol 17 (19-20) ◽  
pp. 2561-2572 ◽  
Author(s):  
Colin Y.L. Woon ◽  
Armin Kraus ◽  
Shyam S. Raghavan ◽  
Brian C. Pridgen ◽  
Kai Megerle ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Three Dimensional ◽  
Tendon Tissue ◽  
Tendon Tissue Engineering ◽  
Human Tendon

scholarly journals Biodegradable Microfluidic Scaffolds for Vascular Tissue Engineering

2004 ◽  
Vol 845 ◽  
Author(s):  
C. J. Bettinger ◽  
J. T. Borenstein ◽  
R. S. Langer
Keyword(s):  
Tissue Engineering ◽  
Vascular Tissue ◽  
Time Lag ◽  
Three Dimensional ◽  
Single Layer ◽  
Vascular Tissue Engineering ◽  
Engineering Applications ◽  
Organ Regeneration ◽  
Superior Mechanical Properties ◽  
Microfabrication Techniques

ABSTRACTThis work describes the integration of novel microfabrication techniques for vascular tissue engineering applications in the context of a novel biodegradable elastomer. The field of tissue engineering and organ regeneration has been borne out of the high demand for organ transplants. However, one of the critical limitations in regeneration of vital organs is the lack of an intrinsic blood supply. This work expands on the development of scaffolds for vascular tissue engineering applications by employing microfabrication techniques. Unlike previous efforts, this work focuses on fabricating single layer and three-dimensional scaffolds from poly(glycerol-sebacate) (PGS), a novel biodegradable elastomer with superior mechanical properties. The transport properties of oxygen and carbon dioxide in PGS were measured through a series of time-lag diffusion experiments. The results of these measurements were used to calculate a characteristic length scale for oxygen diffusion limits in solid PGS scaffolds. Single layer and three-dimensional microfluidic scaffolds were then produced using fabrication techniques specific for PGS. This work has resulted in the fabrication of solid PGS-based scaffolds with biomimetic fluid flow and capillary channels on the order of 10 microns in width. Fabrication of complex, three-dimensional microfluidic PGS scaffolds was also demonstrated by stacking and bonding multiple microfluidic layers.

Structural Optimization of Woven Fabric with Curved Surface

2012 ◽  
Vol 443-444 ◽  
pp. 408-411
Author(s):  
Yan Fang Wang ◽  
Xing Feng Guo
Keyword(s):  
Structural Optimization ◽  
Shear Deformation ◽  
Three Dimensional ◽  
Single Layer ◽  
Woven Fabrics ◽  
Woven Fabric ◽  
Curved Surface ◽  
Theoretical Formula ◽  
Curved Surfaces

The woven fabric with curved surfaces is a kind of single layer woven fabrics, which was produced to smoothly fit three-dimensional solids. The warp or weft of the winding fabric bend were normally made with different lengths, which may result in shear deformation in many cases and accordingly twisting the structure of the fabric after fitted onto the solid. In order to solve the problem mentioned above, a theoretical formula was used to calculate the optimal intervals of the pick-spacing and an improved structure thus was developed in this study.

Three dimensional simulation of air permeability of single layer woven structures

2011 ◽  
Vol 1 (4) ◽  
Author(s):  
Radostina Angelova ◽  
Peter Stankov ◽  
Iskra Simova ◽  
Idoya Aragon
Keyword(s):  
Cross Sections ◽  
Three Dimensional ◽  
Single Layer ◽  
Woven Fabrics ◽  
Transverse Permeability ◽  
Woven Structures ◽  
Dimensional Simulation ◽  
Jet Cross Sections ◽  
Woven Structure

AbstractThe paper deals with a CFD based study of the transverse permeability of a textile woven structure. The reported numerical investigation is preconditioned by both previous experimental and CFD study on jet systems. It is also based on detailed experimental investigation of the porous structure of single layer woven fabrics, made of staple fiber yarns. The flow in through-thickness direction of the woven structures is presented as jet systems, issuing from set of orifices. Two different types of jet system (3×3 jets and 5×5 jets) with two types of jet cross sections (square and circular), corresponding to two different woven structures, are simulated. An analysis is made in terms of the structure of the woven fabrics (area and shape of the interstices between the threads), the parameters of the flow passing through the textile (velocity profiles and velocity fields through isosurfaces), the role of the type of the jet systems, representing the flow and the influence of the shape of the interstices between the threads on the flow pattern. It was found that the applied approach could be effectively used for studying of the transverse permeability of the woven fabrics.

Electrospun fibers/branched-clusters as building units for tissue engineering

2017 ◽  
Vol 1 (1) ◽  
Author(s):  
Benjamin A. Minden-Birkenmaier ◽  
Gretchen S. Selders ◽  
Kasyap Cherukuri ◽  
Gary L. Bowlin
Keyword(s):  
Tissue Engineering ◽  
Tissue Regeneration ◽  
Fiber Diameter ◽  
Drug Loading ◽  
Three Dimensional ◽  
Dermal Fibroblasts ◽  
Polymer Composition ◽  
Electrospun Fibers ◽  
Branch Lengths ◽  
Normal Human

AbstractAlthough electrospun templates are effective at mimicking the extracellular matrix (ECM) of native tissue due to the tailorability of parameters such as fiber diameter, polymer composition, and drug loading, these templates are often limited with regards to cell infiltration and the tailorability of the microenvironments within the structures. Thus, there remains a need for a flexible threedimensional template system which could be combined with cell suspensions to promote three-dimensional tissue regeneration, and ultimately allow cells to freely reorganize and modify their microenvironment. In this study, a mincing process was designed and optimized to create mixtures of electrospun fibers/branched-clusters for use as fundamental tissue engineering building units. These fiber/branched-cluster elements were characterized with regards to fiber and branch lengths, and a method was optimized to combine them with normal human dermal fibroblasts (nHDFs) in culture to create interconnected template constructs. Sectioning and imaging of these constructs revealed cell/fiber integration as well as even cell distribution throughout the construct interior. These fiber/branched-cluster elements represent an innovative flexible tissue regeneration template system.

Electro-spun PLA-PEG-yarns for tissue engineering applications

2018 ◽  
Vol 63 (3) ◽  
pp. 231-243 ◽  
Author(s):  
Magnus Kruse ◽  
Marc Greuel ◽  
Franziska Kreimendahl ◽  
Thomas Schneiders ◽  
Benedict Bauer ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Tensile Strength ◽  
Endothelial Cells ◽  
Fiber Diameter ◽  
Three Dimensional ◽  
Textile Fabrics ◽  
Yarn Diameter ◽  
Spun Yarn ◽  
Spun Yarns ◽  
Woven Structures

Abstract Electro-spinning is widely used in tissue-engineered applications mostly in form of non-woven structures. The development of e-spun yarn opens the door for textile fabrics which combine the micro to nanoscale dimension of electro-spun filaments with three-dimensional (3D) drapable textile fabrics. Therefore, the aim of the study was the implementation of a process for electro-spun yarns. Polylactic acid (PLA) and polyethylene glycol (PEG) were spun from chloroform solutions with varying PLA/PEG ratios (100:0, 90:10, 75:25 and 50:50). The yarn samples produced were analyzed regarding their morphology, tensile strength, water uptake and cytocompatibility. It was found that the yarn diameter decreased when the funnel collector rotation was increasd, however, the fiber diameter was not influenced. The tensile strength was also found to be dependent on the PEG content. While samples composed of 100% PLA showed a tensile strength of 2.5±0.7 cN/tex, the tensile strength increased with a decreasing PLA content (PLA 75%/PEG 25%) to 6.2±0.5 cN/tex. The variation of the PEG content also influenced the viscosity of the spinning solutions. The investigation of the cytocompatibility with endothelial cells was conducted for PLA/PEG 90:10 and 75:25 and indicated that the samples are cytocompatible.

In vitrotwo-dimensional and three-dimensional tenocyte culture for tendon tissue engineering

2013 ◽  
Vol 10 (3) ◽  
pp. E216-E226 ◽  
Author(s):  
Yiwei Qiu ◽  
Xiao Wang ◽  
Yaonan Zhang ◽  
Andrew J. Carr ◽  
Liwei Zhu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Three Dimensional ◽  
Tendon Tissue ◽  
Tendon Tissue Engineering

"HONEYCOMB FILMS": BIOINTERFACE FOR TISSUE ENGINEERING

2002 ◽  
Vol 01 (05n06) ◽  
pp. 415-418 ◽  
Author(s):  
TAKEHIRO NISHIKAWA ◽  
KEIKO ARAI ◽  
JUNKO HAYASHI ◽  
MASAHIKO HARA ◽  
MASATSUGU SHIMOMURA
Keyword(s):  
Tissue Engineering ◽  
Three Dimensional ◽  
Single Layer ◽  
Amphiphilic Polymer ◽  
The Self ◽  
Double Layers ◽  
Tissue Formation ◽  
Moist Air ◽  
Microporous Polymer ◽  
Flat Film

We report that tissue-like structure can be formed when cells are cultured on a microporous polymer film (honeycomb film). The honeycomb films were fabricated by applying a moist air to a spread polymer solution containing biodegradable polymers (poly(L-lactic acid) (PLLA) and poly(ε-caprolactone) (PCL)) and an amphiphilic polymer. Hepatocytes were cultured on a self-supporting honeycomb film of PLLA. The hepatocytes formed a single layer of columnar shape cells with a thickness of 20 μm. The tissue formation of hepatocytes was specifically occurred on the honeycomb film of PLLA and not on a flat film of PLLA. Three-dimensional tissue structures were formed, when cells were cultured on both sides of the self-supporting honeycomb film. Double layers of hepatocytes were obtained by the method. Striated tissues such as heart and blood vessel could be reconstructed by utilizing a stretched honeycomb film of PCL.

Sign in / Sign up

Export Citation Format

Share Document