Electro-spun PLA-PEG-yarns for tissue engineering applications

2018 ◽  
Vol 63 (3) ◽  
pp. 231-243 ◽  
Author(s):  
Magnus Kruse ◽  
Marc Greuel ◽  
Franziska Kreimendahl ◽  
Thomas Schneiders ◽  
Benedict Bauer ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Tensile Strength ◽  
Endothelial Cells ◽  
Fiber Diameter ◽  
Three Dimensional ◽  
Textile Fabrics ◽  
Yarn Diameter ◽  
Spun Yarn ◽  
Spun Yarns ◽  
Woven Structures

Abstract Electro-spinning is widely used in tissue-engineered applications mostly in form of non-woven structures. The development of e-spun yarn opens the door for textile fabrics which combine the micro to nanoscale dimension of electro-spun filaments with three-dimensional (3D) drapable textile fabrics. Therefore, the aim of the study was the implementation of a process for electro-spun yarns. Polylactic acid (PLA) and polyethylene glycol (PEG) were spun from chloroform solutions with varying PLA/PEG ratios (100:0, 90:10, 75:25 and 50:50). The yarn samples produced were analyzed regarding their morphology, tensile strength, water uptake and cytocompatibility. It was found that the yarn diameter decreased when the funnel collector rotation was increasd, however, the fiber diameter was not influenced. The tensile strength was also found to be dependent on the PEG content. While samples composed of 100% PLA showed a tensile strength of 2.5±0.7 cN/tex, the tensile strength increased with a decreasing PLA content (PLA 75%/PEG 25%) to 6.2±0.5 cN/tex. The variation of the PEG content also influenced the viscosity of the spinning solutions. The investigation of the cytocompatibility with endothelial cells was conducted for PLA/PEG 90:10 and 75:25 and indicated that the samples are cytocompatible.

In vitro Three Dimensional Scaffold-free Construct of Human Adipose-derived Stem Cells in Coculture with Endothelial Cells and Fibroblasts

2017 ◽  
Vol 68 (6) ◽  
pp. 1341-1344
Author(s):  
Grigore Berea ◽  
Gheorghe Gh. Balan ◽  
Vasile Sandru ◽  
Paul Dan Sirbu
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Endothelial Cells ◽  
Single Cell ◽  
Cell Line ◽  
Bone Repair ◽  
Umbilical Vein ◽  
Human Fibroblasts ◽  
Three Dimensional ◽  
Adipose Derived Stem Cells

Complex interactions between stem cells, vascular cells and fibroblasts represent the substrate of building microenvironment-embedded 3D structures that can be grafted or added to bone substitute scaffolds in tissue engineering or clinical bone repair. Human Adipose-derived Stem Cells (hASCs), human umbilical vein endothelial cells (HUVECs) and normal dermal human fibroblasts (NDHF) can be mixed together in three dimensional scaffold free constructs and their behaviour will emphasize their potential use as seeding points in bone tissue engineering. Various combinations of the aforementioned cell lines were compared to single cell line culture in terms of size, viability and cell proliferation. At 5 weeks, viability dropped for single cell line spheroids while addition of NDHF to hASC maintained the viability at the same level at 5 weeks Fibroblasts addition to the 3D construct of stem cells and endothelial cells improves viability and reduces proliferation as a marker of cell differentiation toward osteogenic line.

scholarly journals A Three-Dimensional Collagen-Elastin Scaffold for Heart Valve Tissue Engineering

2018 ◽  
Vol 5 (3) ◽  
pp. 69 ◽  
Author(s):  
Xinmei Wang ◽  
Mir Ali ◽  
Carla Lacerda
Keyword(s):  
Tissue Engineering ◽  
Endothelial Cells ◽  
Heart Valves ◽  
Current Knowledge ◽  
Three Dimensional ◽  
Stimuli Responsive ◽  
Integrin Β1 ◽  
Mesenchymal Transition ◽  
3D Collagen

Since most of the body’s extracellular matrix (ECM) is composed of collagen and elastin, we believe the choice of these materials is key for the future and promise of tissue engineering. Once it is known how elastin content of ECM guides cellular behavior (in 2D or 3D), one will be able to harness the power of collagen-elastin microenvironments to design and engineer stimuli-responsive tissues. Moreover, the implementation of such matrices to promote endothelial-mesenchymal transition of primary endothelial cells constitutes a powerful tool to engineer 3D tissues. Here, we design a 3D collagen-elastin scaffold to mimic the native ECM of heart valves, by providing the strength of collagen layers, as well as elasticity. Valve interstitial cells (VICs) were encapsulated in the collagen-elastin hydrogels and valve endothelial cells (VECs) cultured onto the surface to create an in vitro 3D VEC-VIC co-culture. Over a seven-day period, VICs had stable expression levels of integrin β1 and F-actin and continuously proliferated, while cell morphology changed to more elongated. VECs maintained endothelial phenotype up to day five, as indicated by low expression of F-actin and integrin β1, while transformed VECs accounted for less than 7% of the total VECs in culture. On day seven, over 20% VECs were transformed to mesenchymal phenotype, indicated by increased actin filaments and higher expression of integrin β1. These findings demonstrate that our 3D collagen-elastin scaffolds provided a novel tool to study cell-cell or cell-matrix interactions in vitro, promoting advances in the current knowledge of valvular endothelial cell mesenchymal transition.

Preliminary Study for Co-Culture of Osteoblasts and Endothelial Cells to Construct the Regenerative Bone

2017 ◽  
Vol 758 ◽  
pp. 269-272 ◽  
Author(s):  
Michiyo Honda ◽  
Mamoru Aizawa
Keyword(s):  
Tissue Engineering ◽  
Endothelial Cells ◽  
Bone Development ◽  
Three Dimensional ◽  
Bone Defects ◽  
Culture Conditions ◽  
Bone Diseases ◽  
Promising Strategy ◽  
Novel Treatment ◽  
Preliminary Study

Vascularization is a crucial process during bone development and regeneration. A number of studies have shown that the interaction between osteoblasts and endothelial cells plays a key role in osteogenesis by using co-culture system. However, vascularization strategies in cell-based bone tissue engineering depend on optimal culture conditions. In this study, we determined the optimal co-culture conditions in view of osteogenic parameters and examined the effects of angiogenic properties on osteogenesis. As for cell proliferation, the proportion of osteoblasts increased and that of endothelial cells decreased as culture period passed. Assessment of osteogenic differentiation shows that co-culture of osteoblasts and endothelial cells significantly increased alkaline phosphatase activity and expression of bone-related genes. Furthermore, abundant microcapillary-like structures were observed which endothelial cells self-assembled into branches and net-like structures. The use of endothelial cells would be a promising strategy to promote vascularization to support the bone regeneration. Combination of these cell-based approaches and tissue engineering like three-dimensional scaffolds could provide a novel treatment therapy for bone defects and bone diseases.

Electrospun fibers/branched-clusters as building units for tissue engineering

2017 ◽  
Vol 1 (1) ◽  
Author(s):  
Benjamin A. Minden-Birkenmaier ◽  
Gretchen S. Selders ◽  
Kasyap Cherukuri ◽  
Gary L. Bowlin
Keyword(s):  
Tissue Engineering ◽  
Tissue Regeneration ◽  
Fiber Diameter ◽  
Drug Loading ◽  
Three Dimensional ◽  
Dermal Fibroblasts ◽  
Polymer Composition ◽  
Electrospun Fibers ◽  
Branch Lengths ◽  
Normal Human

AbstractAlthough electrospun templates are effective at mimicking the extracellular matrix (ECM) of native tissue due to the tailorability of parameters such as fiber diameter, polymer composition, and drug loading, these templates are often limited with regards to cell infiltration and the tailorability of the microenvironments within the structures. Thus, there remains a need for a flexible threedimensional template system which could be combined with cell suspensions to promote three-dimensional tissue regeneration, and ultimately allow cells to freely reorganize and modify their microenvironment. In this study, a mincing process was designed and optimized to create mixtures of electrospun fibers/branched-clusters for use as fundamental tissue engineering building units. These fiber/branched-cluster elements were characterized with regards to fiber and branch lengths, and a method was optimized to combine them with normal human dermal fibroblasts (nHDFs) in culture to create interconnected template constructs. Sectioning and imaging of these constructs revealed cell/fiber integration as well as even cell distribution throughout the construct interior. These fiber/branched-cluster elements represent an innovative flexible tissue regeneration template system.

Preparation of three-dimensional multilayer ECM-simulated woven/knitted fabric composite scaffolds for potential tissue engineering applications

2019 ◽  
Vol 90 (7-8) ◽  
pp. 925-936 ◽  
Author(s):  
Dandan Guo ◽  
Shuai Wang ◽  
Yuxiang Yin ◽  
Jun Luo ◽  
Chenjie Meng ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Pore Diameter ◽  
Fiber Diameter ◽  
Three Dimensional ◽  
Single Layer ◽  
Woven Fabrics ◽  
Collagen Solution ◽  
Tendon Tissue Engineering ◽  
Bonding Properties ◽  
Composite Fabrics

The extracellular matrix (ECM), with its multilayer fiber structure, regulates diverse functions including cell proliferation, migration, differentiation and tissue regeneration effects. To mimic and replace the native ECM, the structures and properties of three single-layer fabric substrates including warp/weft-knitted and woven fabrics were analyzed, then two-layer warp/weft-knitted composite fabrics prepared by polyurethane (PU) bonding, and woven composite fabrics prepared by polycaprolactone (PCL)/collagen solution bonding or PU bonding, were studied. After PCL/collagen solution bonding or PU bonding, properties such as pore diameter, air permeability, stress and the contact angle of composite fabrics decreased by some degree, while fiber diameter, thickness and the thermal conductivity of composite fabrics increased. In combination with fiber diameter, pore diameter and physical properties, we know that warp- or weft-knitted composite fabrics are ideal scaffolda for potential applications in nerve, myocardium and tendon tissue engineering.

scholarly journals Liquid-Assisted Electrospinning Three-Dimensional Polyacrylonitrile Nanofiber Crosslinked with Chitosan

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Xiaoli Yang ◽  
Xue Chen ◽  
Jingyi Zhao ◽  
Wenlu Lv ◽  
Qilu Wu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Wound Healing ◽  
Pore Structure ◽  
Antibacterial Effect ◽  
Fiber Diameter ◽  
Three Dimensional ◽  
Special Structure ◽  
Tissue Engineering Scaffolds ◽  
Antimicrobial Effectiveness ◽  
Pan Nanofiber

Electrospinning has become a popular nanotechnology for the fabrication of tissue engineering scaffolds, which can precisely regulate fiber diameter and microstructure. Herein, we have prepared a three-dimensional polyacrylonitrile (PAN) nanofiber by liquid-assisted electrospinning. The spacing between PAN nanofibers can reach to 15-20 μm, as the uniform internally connected pore structure can be formed, through the regulation of parameters. Furthermore, the chitosan attached to the as-prepared nanofibers gives the material antibacterial effect and increases its biocompatibility. Meanwhile, the special structure of chitosan also provides the possibility for further loading drugs in dressings in the future. This newly developed nanocomposite seems to be highly suitable for wound healing due to its unique properties of biodegradability, biocompatibility, and antimicrobial effectiveness.

HYDROXYAPATITE, ALGINATE, AND CHITOSAN FOR BONE SCAFFOLDS: SPECTROSCOPY STUDY

2016 ◽  
Vol 19 (2) ◽  
pp. 93-100
Author(s):  
Lalita El Milla
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Functional Groups ◽  
Bone Growth ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Tissue Engineering Scaffolds ◽  
Hydroxyapatite Powder ◽  
Materials Used

Scaffolds is three dimensional structure that serves as a framework for bone growth. Natural materials are often used in synthesis of bone tissue engineering scaffolds with respect to compliance with the content of the human body. Among the materials used to make scafffold was hydroxyapatite, alginate and chitosan. Hydroxyapatite powder obtained by mixing phosphoric acid and calcium hydroxide, alginate powders extracted from brown algae and chitosan powder acetylated from crab. The purpose of this study was to examine the functional groups of hydroxyapatite, alginate and chitosan. The method used in this study was laboratory experimental using Fourier Transform Infrared (FTIR) spectroscopy for hydroxyapatite, alginate and chitosan powders. The results indicated the presence of functional groups PO43-, O-H and CO32- in hydroxyapatite. In alginate there were O-H, C=O, COOH and C-O-C functional groups, whereas in chitosan there were O-H, N-H, C=O, C-N, and C-O-C. It was concluded that the third material containing functional groups as found in humans that correspond to the scaffolds material in bone tissue engineering.

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