Histological Features of the Nasal Passage in Juvenile Japanese White Rabbits

2022 ◽  
pp. 019262332110687
Author(s):  
Yoshinori Yamagiwa ◽  
Masaaki Kurata ◽  
Hiroshi Satoh
Keyword(s):  
Endochondral Ossification ◽  
Vomeronasal Organ ◽  
Goblet Cells ◽  
Olfactory Nerve ◽  
Nasolacrimal Duct ◽  
Respiratory Epithelium ◽  
Basic Knowledge ◽  
Nasal Passage ◽  
Toxicological Evaluation ◽  
Clear Cytoplasm

Rabbits are sometimes used for intranasal toxicology studies. We investigated the postnatal development of the nasal passage in juvenile Japanese white rabbits from just after birth to 6-week-old to provide information for conducting intranasal toxicological evaluation using juvenile animals. On postnatal day (PND) 1, the nasal passage consisted of the septum with mostly cartilaginous nasal wall and turbinates. The lining squamous, transitional, respiratory, and olfactory epithelia were already distributed similar to adults and were still underdeveloped. The nasal passage gradually expanded with age, as did the nasal wall, including the turbinates formed by endochondral ossification. The maxilloturbinate elongated, during which it branched complexly. The respiratory epithelium takes the form of columnar epithelium together with a reduction in goblet cells. In addition, the olfactory epithelium had clear cytoplasm in the ethmoturbinate, the olfactory nerve bundles thickened, and Bowman’s gland acini increased in size and number. Other tissues, including the vomeronasal organ, nasal-associated lymphoid tissue, and nasolacrimal duct, also developed histologically with age. This investigation characterized the postnatal histological development of the nasal passage in Japanese white rabbits, providing basic knowledge regarding the histological examination and rationale for appropriate study design of intranasal toxicology studies in juvenile rabbits.

S282 – Localization of Nitric Oxide Synthase in Nasal Mucosa

2008 ◽  
Vol 139 (2_suppl) ◽  
pp. P169-P169
Author(s):  
Shigetoshi Yoda ◽  
Fukushima Hisaki ◽  
Nishiike Suetaka ◽  
Shibata Dai ◽  
Tamotsu Harada
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Allergic Rhinitis ◽  
Nasal Mucosa ◽  
Olfactory Epithelium ◽  
Vascular Endothelial Cells ◽  
Olfactory Nerve ◽  
Respiratory Epithelium ◽  
Vascular Endothelial ◽  
Oxide Synthase

Objectives Several studies have reported that inducible nitric oxide synthase (iNOS) was expressed within the epithelial cell of the trachea in asthmic patients and asthmic model animals. However, neither appearance nor localization of iNOS in the nasal mucosa of allergic rhinitis has been examined. This research clarifies expression and the localization of iNOS in the nasal mucosa of allergic rhinitis by using the allergic model mice. Methods Allergic rhinitis was induced in male mice at 6 weeks of age using purified Japanese cedar pollen allergen (Cry j 1). Cry j 1 was injected 2 times into the abdomen (day 0 and 4) and administered intranasally for 7 consecutive days (day 9–15). On day 22, the expression and localization of iNOS in nasal mucosa of both allergic rhinitis model and control mice were examined by immunohistochemistry. Results In control mice, the expression of iNOS was localized in olfactory nerve, nasal gland beneath the respiratory epithelium and vascular endothelial cells. In allergic rhinitis group, the expression of iNOS was not only localized in olfactory nerve, nasal gland, and vascular endothelial cells, but also in olfactory epithelium and respiratory epithelium. Conclusions The expression of iNOS was increased in olfactory epithelium and respiratory epithelium of allergic rhinitis mice compared with controls.

Leukotriene D4(LTD4) Induces Mucus Secretion from Goblet Cells in the Guinea Pig Respiratory Epithelium

1990 ◽  
Vol 16 (6) ◽  
pp. 711-725 ◽  
Author(s):  
S. T. Hoffstein ◽  
P. E. Malo ◽  
P. Bugelski ◽  
E. B. Wheeldon
Keyword(s):  
Guinea Pig ◽  
Goblet Cells ◽  
Respiratory Epithelium ◽  
Mucus Secretion ◽  
Leukotriene D4

Can an orbital gland function in the vomeronasal sense? A study of the pygopodid Harderian gland

2000 ◽  
Vol 78 (4) ◽  
pp. 648-654 ◽  
Author(s):  
S J Rehorek ◽  
B T Firth ◽  
M N Hutchinson
Keyword(s):  
Vomeronasal Organ ◽  
Harderian Gland ◽  
Nasolacrimal Duct ◽  
Morphological Features ◽  
Direct Link ◽  
Growing Body ◽  
Gland Function

The Harderian gland occurs in the orbit of most tetrapod vertebrates. A growing body of evidence suggests that this gland is associated with the chemoreceptive function of the vomeronasal organ. In the present study, the morphology of the Harderian gland in two species of pygopodids was examined, and the results were contrasted with those from both geckos and snakes. The results show that the pygopodid Harderian gland shares histochemical and ultrastructural features with that of the geckos. However, in several gross morphological features, the pygopodid Harderian gland more closely resemble that of snakes than that of geckos. In both pygopodids and snakes, the nasolacrimal duct forms a direct link between the Harderian gland and the vomeronasal organ, which indicates that this specialized connection between the Harderian gland and vomeronasal organ is a convergent evolutionary attribute of these two groups.

scholarly journals The Terminal and Vomeronasal Nerves of Montpellier Snake, Malpolon monspessulana (Colubridae, Ophidia, Squamata)

2016 ◽  
Vol 50 (2) ◽  
pp. 179-184
Author(s):  
A. R. Omar ◽  
A. I. Dakrory
Keyword(s):  
Vomeronasal Organ ◽  
Olfactory Nerve ◽  
Sensory Epithelium ◽  
Current Work ◽  
The Brain ◽  
Nasal Capsule ◽  
Jacobson's Organ

Abstract The montpellier snake, Malpolon monspessulana, used in the current work in order to study the nervi terminalis and vomeronasalis. The vomeronasal organ or Jacobson’s organ is a part of the olfactory apparatus. This organ is innervated by the terminal and vomeronasal nerves. These nerves emerge from the sensory epithelium of Jacobson’s organ simultaneously. The bundles of the terminal and vomeronasal nerves traverse together the cavity of the nasal capsule in their way to the brain. These nerves bear scattered ganglionic cells which represent the ganglion terminale. They leave the capsule through the foramen olfactorium advehens. The terminal and vomeronasal nerves are connected with the olfactory nerve and enter the olfactory formation of the forebrain.

The Harderian gland of two species of snakes: Pseudonaja textilis (Elapidae) and Thamnophis sirtalis (Colubridae)

2003 ◽  
Vol 81 (3) ◽  
pp. 357-363 ◽  
Author(s):  
Susan J Rehorek ◽  
Mimi Halpern ◽  
Bruce T Firth ◽  
Mark N Hutchinson
Keyword(s):  
Gross Anatomy ◽  
Vomeronasal Organ ◽  
Harderian Gland ◽  
Interspecific Variation ◽  
Nasolacrimal Duct ◽  
Thamnophis Sirtalis ◽  
Mucous Cells ◽  
Vomeronasal System ◽  
Snake Species ◽  
Harderian Glands

The reptilian Harderian gland is a poorly understood cephalic structure. Despite the recent assertion that in snakes it may function as part of the vomeronasal system, the Harderian gland has been described in few snake species. In this study we examined the gross anatomy, histology, and ultrastructure of the Harderian gland of two different advanced snake species (Colubroidea): Pseudonaja textilis (Elapidae) and Thamnophis sirtalis (Colubridae). In both species the Harderian gland is a large serous gland whose secretions pass directly into the vomeronasal organ via the nasolacrimal duct. Contrary to previous publications, the Harderian gland in both species studied possesses a specific duct system lined by mucous cells. However, the Harderian glands of these two species differ in shape, the histochemical nature of these mucous secretions, and the ultrastructure of the serous granules. In conclusion, though the Harderian glands of snakes are remarkably conserved morphologically, there is some interspecific variation.

Ultrastructure of the Middle Ear Mucosa

1976 ◽  
Vol 85 (2_suppl) ◽  
pp. 30-35 ◽  
Author(s):  
Erwin Hentzer
Keyword(s):  
Middle Ear ◽  
Secretory Granules ◽  
Goblet Cells ◽  
Respiratory Epithelium ◽  
Secretory Cells ◽  
Secretory Product ◽  
Ciliated Cells ◽  
Loose Connective Tissue ◽  
Middle Ear Mucosa ◽  
Subepithelial Layer

Histologic studies of recent years have definitely concluded that the epithelium of the middle ear is a modified respiratory epithelium with ciliated and secretory cells, including goblet cells. These cells, like the ciliated cells, are developd from the basal cell and are just ordinary secretory cells completely filled with secretory granules. For different reasons it is postulated, that the dark secretory granules are prestages of the actual secretory product presenting as light granules and that dark and light granules do not represent different secretory capacities of the cell. The subepithelial layer which consists of loose connective tissue is a structure of just as great importance as the epithelial layer.

The Nasolabial Cyst: A Nonodontogenic Oral Cyst Related to Nasolacrimal Duct Epithelium

2011 ◽  
Vol 135 (11) ◽  
pp. 1499-1503 ◽  
Author(s):  
Yanelba Toribio ◽  
Michael H.A. Roehrl
Keyword(s):  
Current Knowledge ◽  
Squamous Metaplasia ◽  
Treatment Options ◽  
Basal Layer ◽  
Inflammatory Cells ◽  
Goblet Cells ◽  
Nasolacrimal Duct ◽  
Secretory Cells ◽  
Cytokeratin 5 ◽  
Cyst Lining

Nasolabial cysts are interesting, relatively uncommon benign extraosseous maxillary lesions. We review current knowledge about epidemiology, symptoms, imaging modalities, pathogenesis, histopathologic and ultrastructural features, treatment options, and prognosis. Nasolabial cyst lining epithelium is characteristically composed of a basal layer of cuboidal cells and a luminal layer of columnar secretory cells with interspersed mucous goblet cells. In addition, areas of multilayered epithelium and squamous metaplasia may be seen. The cyst stroma is characterized by collagen-rich fibrovascular tissue with variably admixed chronic inflammatory cells. Furthermore, to our knowledge, we report the first example of immunohistochemical protein expression profiling of nasolabial cyst lining epithelium, discovering that basal layer cells express p63 and cytokeratin 5/6, while goblet cells express MUC-2 and MUC-5AC mucins, supporting the notion that nasolabial cysts can be understood as hamartomatous, locally expansile remnants of distal nasolacrimal duct development.

scholarly journals Structure of Masera's Septal Olfactory Organ in Cat (Felis silvestris f. catus) - Light Microscopy in Selected Stages of Ontogeny

2006 ◽  
Vol 75 (4) ◽  
pp. 471-475
Author(s):  
I. Kociánová ◽  
A. Gorošová ◽  
F. Tichý ◽  
P. Čížek ◽  
M. Machálka
Keyword(s):  
Nasal Cavity ◽  
Developmental Stage ◽  
Vomeronasal Organ ◽  
Sensory Epithelium ◽  
Respiratory Epithelium ◽  
Domestic Cat ◽  
Olfactory Organ ◽  
Felis Silvestris ◽  
Typical Structure ◽  
Surface Appearance

The septal organ /SO/ (Masera's organ /MO/) is a chemoreceptor presently considered one of three types of olfactory organs (along with the principal olfactory region and vomeronasal organ). Notwithstanding the septal organ having been first described by Rodolfo Masera in 1943, little is known of the properties of sensory neurons or of its functional significance in chemoreception. Until now the septal organ has been described only in laboratory rodents and some marsupials. This work refers to its existence in the domestic cat (Felis silvestris f. catus). The septal organ can be identified at the end of embryonic period - 27 or 28 days of ontogenesis in cats (the 6th developmental stage of Štěrba) - coincident with formation of the principal olfactory region in nasal cavity. At 45 days of ontogenesis (the 9th developmental stage of Štěrba), this septal olfactory organ is of circular or oval shape, 120 μm in diameter, in ventral part of septum nasi, lying caudally to the opening of ductus incisivus. The structure of the epithelium of septal olfactory organ is clearly distinct from the respiratory epithelium of the nasal cavity. It varies in thickness, cellular composition, as well as free surface appearance, and even lack the typical structure of sensory epithelium, in this developmental period. Nerve bundles and glandular acini are lacking in the lamina propria mucosae of the septal organ and in the adjacent tissues. Glands appear as the single non-luminized cords of epithelia extending from the surface. The adjacent respiratory epithelium contains numerous goblet cells.

Passage of Harderian gland secretions to the vomeronasal organ of Thamnophis sirtalis (Serpentes: Colubridae)

2000 ◽  
Vol 78 (7) ◽  
pp. 1284-1288 ◽  
Author(s):  
S J Rehorek ◽  
W J Hillenius ◽  
W Quan ◽  
M Halpern
Keyword(s):  
Vomeronasal Organ ◽  
Harderian Gland ◽  
Nasolacrimal Duct ◽  
Sensory Epithelium ◽  
Thamnophis Sirtalis ◽  
Vomeronasal System ◽  
Treatment Groups ◽  
Terrestrial Vertebrates ◽  
Rich Solution ◽  
The Right

The Harderian gland is a poorly understood structure found in the anterior orbit of most terrestrial vertebrates. In colubrid snakes it is a seromucous gland with a large postorbital portion. Numerous functions have been ascribed to this gland, including contributions to orbital lubrication or the vomeronasal system. Anatomically the Harderian gland is connected to the vomeronasal organ (VNO) via the nasolacrimal duct. In this study we traced the serous secretions of the Harderian gland of two subspecies of Thamnophis sirtalis (Colubridae), using autoradiographic techniques at the light-microscopic level. We injected the Harderian gland of the snakes with H3-proline either unilaterally (right side) or bilaterally. The right Harderian glands of both treatment groups were then injected with a potassium-rich solution. No labeling was observed in the orbital space of any treatment group, suggesting that the Harderian gland secretions of T. sirtalis do not function in orbital lubrication. Labeling was only observed in the right Harderian gland, Harderian gland ducts, nasolacrimal duct, apical vomeronasal sensory epithelium, VNO lumen, and vomeronasal duct. No such labeling was observed in any of the other treatments examined. Thus, the serous secretions of the Harderian gland in snakes flow to the VNO, and may be considered part of the vomeronasal system. The specific function of the Harderian gland secretions in the vomeronasal system remains to be determined.

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