scholarly journals Spermatid Giant Cells, Tubular Hypospermatogenesis, Spermatogonial Swelling, Cytoplasmic Vacuoles, and Tubular Dilatation in the Testes of Normal Rabbits

1986 ◽  
Vol 23 (2) ◽  
pp. 176-183 ◽  
Author(s):  
D. Morton ◽  
S. E. Weisbrode ◽  
W. E. Wyder ◽  
J. K. Maurer ◽  
C. C. Capen
Keyword(s):  
Electron Microscopy ◽  
Light And Electron Microscopy ◽  
Age Groups ◽  
Giant Cells ◽  
Seminiferous Tubules ◽  
Intercellular Bridges ◽  
Nuclear Membranes ◽  
Cytoplasmic Vacuoles ◽  
New Zealand White Rabbits ◽  
Tubular Dilatation

Testes of 36 normal New Zealand white rabbits (8, 15, 18, 26, and greater than 52 weeks of age) were examined by light and electron microscopy. The incidence and number of affected tubules were determined for spermatid giant cells, focal tubular hypospermatogenesis, cytoplasmic swelling of spermatogonia, intracytoplasmic vacuoles in seminiferous epithelium, and tubular dilatation. Spermatogenesis commenced at 15–18 weeks of age and was present in all rabbits by 18 weeks. Spermatid giant cells occurred in 96% of rabbits 15 weeks of age and older. Focal hypospermatogenesis was present in 14–57% of testes once active spermatogenesis began. Ninety-seven percent of testes in all age groups combined had spermatogonial swelling. Infrequent dilated seminiferous tubules were present in five rabbits. Ultrastructurally, spermatid giant cells were round cells with multiple nuclei that appeared to arise by widening of narrow intercellular bridges that normally connect spermatogenic epithelial cells. Pale-staining spermatogonia consisted of cytoplasmic and nuclear swelling with disruption of plasma and nuclear membranes. Tubules with spermatogonial swelling were more numerous in 15- and 18-week-old rabbits. There were no significant differences in incidence or extent of spermatid giant cells, focal hypospermatogenesis, cytoplasmic vacuoles, or tubular dilatation between age groups after spermatogenesis commenced. Although the cause of these changes is not known, they were frequent findings in normal rabbits 15 weeks of age and older.

Spermiogenesis and testicular cycle of the lizard Tropidurus torquatus (Squamata, Tropiduridae) in the Cerrado of central Brazil

2001 ◽  
Vol 22 (2) ◽  
pp. 217-233 ◽  
Author(s):  
Guarino Colli ◽  
Gustavo H.C. Vieira ◽  
Sônia Báo ◽  
Helga Wiederhecker
Keyword(s):  
Electron Microscopy ◽  
Chromatin Condensation ◽  
Light And Electron Microscopy ◽  
Low Frequency ◽  
Seminiferous Tubules ◽  
Fibrous Sheath ◽  
Central Brazil ◽  
Sheath Formation ◽  
Reproductive Cycles ◽  
Regenerative Phase

AbstractWe studied the spermiogenesis and testicular cycle of the lizard Tropidurus torquatus, using light and electron microscopy. Males bearing spermatozoa were present practically year-round and spermatogenic activity showed a regenerative phase from late dry season to the end of the rainy season (July to March), with low frequency of initial stages of the spermatogenic cycle, and a brief degenerative phase from April to June, lacking the total regression of seminiferous tubules. These characteristics resemble those from species with continuous reproductive cycles, contrasting with the strongly seasonal reproductive cycle of females. Spermiogenesis includes nuclear elongation, chromatin condensation, acrosomal and flagellar development, and elimination of excessive cytoplasm. We describe some new aspects in the spermiogenesis of T. torquatus, including the interaction between spermatid and Sertoli cell, acrosomal granule, subacrosomal granule, and the fibrous sheath formation. The testicular cycle of T. torquatus is very similar to that of other lizards that inhabit seasonal environments, and its spermiogenesis and ultrastructure of mature sperm display a number of conservative features.

The postnatal maturation of efferent tubules in the rat: a light and electron microscopy study

Development ◽  
1986 ◽  
Vol 96 (1) ◽  
pp. 51-63
Author(s):  
Sandro Francavilla ◽  
Sergio Moscardelli ◽  
Bernardino Bruno ◽  
Patrizio Scorza Barcellona ◽  
Cesare De Martino
Keyword(s):  
Electron Microscopy ◽  
Fluid Transport ◽  
Light And Electron Microscopy ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Seminiferous Tubules ◽  
Genital Duct ◽  
Postnatal Maturation ◽  
Postnatal Differentiation ◽  
Tubule Wall

The postnatal maturation of the epithelium and tubule wall of efferent tubules in the rat was investigated by light and transmission electron microscopy, from birth to 50 days of age, when sperms were released from the seminiferous tubules and appeared in the genital duct. At the end of the first week of life, an endocytotic apparatus is differentiated in the epithelial cells. During the third week of life, efferent tubules developed specializations for the transport of sperms and fluids, namely the appearance of ciliated elements interspersed among the principal cells of the epithelium, and differentiation of myoid elements in the tubule wall. The appearance of specializations related to endocytosis and fluid transport across the epithelium preceded the canalization of the seminiferous cords which, in fact, is reported to appear at the end of the second week of life in the rat, along with the initial secretion of testicular fluid. This suggested that the maturation of efferent tubules is not triggered by the passage of testicular fluid, as surmised for the postnatal differentiation of caput epididymis. The postnatal maturation of efferent tubules was almost complete 35 days after birth. The appearance of sperms in the genital duct of 50-day-old animals was not associated with any remarkable structural change.

The Ultrastructure of Malignant Fibrous Histiocytoma (Case Report)

1977 ◽  
Vol 63 (4) ◽  
pp. 387-396 ◽  
Author(s):  
Luciano Lombardi ◽  
Silvana Pilotti ◽  
Antonino Carbone ◽  
Franco Rilke
Keyword(s):  
Electron Microscopy ◽  
Stem Cells ◽  
Case Report ◽  
Soft Tissue ◽  
Malignant Fibrous Histiocytoma ◽  
Fibrous Histiocytoma ◽  
Light And Electron Microscopy ◽  
Giant Cells ◽  
Multinucleated Giant Cells ◽  
Cellular Types

A soft tissue malignant fibrous histiocytoma was studied by light and electron microscopy. The presence of fibroblast-like cells, histiocyte-like cells, undifferentiated stem cells, and xanthomatous cells was confirmed. Fibroblast- and histiocyte-like multinucleated giant cells were also observed. The observation of lysosomes in some fibroblast-like cells, the features of undifferentiated stem cells, and the presence of cells with intermediate characteristics common to xanthomatous, histiocytic, and stem cells suggest a strict relationship among these cellular types.

Feline Mammary Sarcoma Composed of Cells Resembling Myofibroblasts

1986 ◽  
Vol 23 (2) ◽  
pp. 118-124 ◽  
Author(s):  
D. W. Hayden ◽  
H. K. Ghobrial ◽  
K. H. Johnson ◽  
L. C. Buoen
Keyword(s):  
Electron Microscopy ◽  
Tumor Cells ◽  
Light And Electron Microscopy ◽  
Giant Cells ◽  
Domestic Cat ◽  
Cell Junctions ◽  
Spindle Cells ◽  
Predominant Type ◽  
Epithelial Origin ◽  
Specific Labelling

A recurrent mammary sarcoma from an 11-year-old, female domestic cat was studied by light and electron microscopy and immunohistochemistry. The tumor consisted of interlacing bands of spindle cells with elongated blunt-ended nuclei and variable amounts of stroma. Multinucleated tumor giant cells and mitoses were common. Ultrastructurally, tumor cells had abundant rough endoplasmic reticulum with dilated cisternae, a prominent Golgi complex, frequent mitochondria, bundles of intracytoplasmic filaments with focal densities, and discontinuous basal lamina-like material and cell junctions. These findings suggest that myofibroblast-like cells were the predominant type of tumor cell. Failure to demonstrate immunoreactivity for cytokeratins attested to the non-epithelial origin of these neoplastic cells. Uniform immunoreactivity with anti-vimentin antibodies and specific labelling of some tumor cells with antiserum to actin are compatible with an origin of this tumor from modified fibroblasts (i.e., myofibroblast-like cells). Tumors composed largely of myofibroblasts may be unique and warrant separate classification from other types of fibroblastic tumors in animals.

Fine structure of the giant cells induced by Meloidogyne javanica in lima bean

1984 ◽  
Vol 62 (3) ◽  
pp. 429-436 ◽  
Author(s):  
F. Fattah ◽  
J. M. Webster
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Light And Electron Microscopy ◽  
Lima Bean ◽  
Meloidogyne Javanica ◽  
Giant Cells ◽  
Egg Laying ◽  
Phaseolus Lunatus ◽  
Wide Range

Giant cells associated with egg-laying females of Meloidogyne javanica in lima bean, Phaseolus lunatus cv. L-136, were examined by light and electron microscopy. These giant cells have characteristics that are typical of nematode-induced giant cells in a wide range of hosts, but they differ in that they (i) are less closely associated with xylem vessels, (ii) contain a very large number of plastids which are devoid of starch grains, and (iii) contain several different forms of cytoplasmic crystals. One form of the crystal is associated with a large number of "spiny" vesicles. The possible role of these features, especially the crystals, in the giant cell response of lima bean is discussed.

Submucosal glands after maxillary sinus surgery. An experimental study in rabbits

1996 ◽  
Vol 110 (7) ◽  
pp. 644-648 ◽  
Author(s):  
Pablo J. Melgarejo-Moreno ◽  
Inmaculada Ribera-Cortada ◽  
Diego Hellin-Meseguer
Keyword(s):  
Electron Microscopy ◽  
Maxillary Sinus ◽  
Light And Electron Microscopy ◽  
Complete Removal ◽  
Sinus Surgery ◽  
Secretory Cells ◽  
Surgical Removal ◽  
New Zealand White Rabbits ◽  
And Control ◽  
Sinus Mucosa

AbstractThirty New Zealand White rabbits underwent unilateral partial or complete removal of maxillary sinus mucosa in order to evaluate submucosal maxillary sinus glands. After three months, specimens were taken for examination from all operated on and control sinuses. Bacteriological cultures, light and electron microscopy were performed. Histopathological findings showed a decrease in the number of serous glands and significant inflammation was present in the sinus in which there was complete surgical removal. Electron microscopy revealed changes in the secretory cells of the serous glands in the regenerated postsurgical mucosa.

scholarly journals Developmental and regressive changes in the testes of the Himalayan rabbit

1992 ◽  
Vol 26 (3) ◽  
pp. 167-179 ◽  
Author(s):  
Ichiro Tsunenari ◽  
Alexander Kast
Keyword(s):  
Age Groups ◽  
Giant Cells ◽  
Mature Male ◽  
Seminiferous Tubules ◽  
Seasonal Differences ◽  
Toxicity Studies ◽  
Age Related ◽  
Testicular Regression ◽  
Regressive Changes

The testes of 81 Himalayan rabbits, controls in toxicity studies, and 113 purpose-bred rabbits of various age groups from birth to 2 years were studied. In mature male rabbits, regressive changes in the seminiferous tubules including the multinucleated spermatid giant cells were common and showed a tendency for age-related and seasonal differences. The finding is considered as a remnant of the previous seasonal and possibly also social testicular regression in wild living animals. The giant cells develop from the syncytium of the germinative epithelium and have parallels in phylogenesis.

scholarly journals ANALYSIS OF STRUCTURAL PLASTICITY IN THE HONEY BEE BRAIN USING THE CAVALIERI ESTIMATOR OF VOLUME AND THE DISECTOR METHOD

2011 ◽  
Vol 19 (2) ◽  
pp. 139 ◽  
Author(s):  
Sheena M Brown ◽  
Ruth MA Napper ◽  
Alison R Mercerl
Keyword(s):  
Electron Microscopy ◽  
Honey Bee ◽  
Antennal Lobe ◽  
Light And Electron Microscopy ◽  
Age Groups ◽  
Structural Plasticity ◽  
Light Microscope Level ◽  
Heterogeneous Distribution ◽  
Data Points ◽  
The Central Nervous System

The antennal lobe of the worker honey bee has been used as a model system to address the origins of structural plasticity in the central nervous system. A combination of light and electron microscopy was used to determine total synapse number within an easily identifiable sub-unit of the antennal lobe neuropil, the T4-2(1) glomerulus. The Cavalieri method was applied at the light microscope level to determine a reference volume (Vref) of this glomerulus. Using transmission electron microscopy, the physical disector was used to determine synaptic density (Nv) within the T4-2(1) glomerulus. An estimate of the total synapse number N(syn) was determined by; N(syn) = V(ref) Nv. Newly emerged, 4-day old,10-day old and forager-aged bees were analysed. Results showed that despite a significant increase in T4-2(1) volume with age, the total number of synapses in this glomerulus did not show a corresponding increase. Disturbingly, it is possible that huge variances within age groups, due to one or two outlying data points, could be masking the true trend of synapse number. This variance, the heterogeneous distribution of synapses within this glomerulus and the problems associated with reproducibility of synapse counts are discussed.

scholarly journals Coagulant and Non-Coagulant Fixation of Plant Cells

1973 ◽  
Vol 26 (6) ◽  
pp. 1231 ◽  
Author(s):  
TP O'brien ◽  
J Kuo ◽  
M E Mccully ◽  
S-Y Zee
Keyword(s):  
Electron Microscopy ◽  
Phaseolus Vulgaris ◽  
Cell Membrane ◽  
Light And Electron Microscopy ◽  
Plant Cells ◽  
Living Cells ◽  
Root Tip ◽  
Cytoplasmic Vacuoles ◽  
Heracleum Mantegazzianum

The appearance of cells from the meristem of the root tip of Phaseolus vulgaris after fixation in a variety of coagulant and non-coagulant fixatives is described and illustrated by correlated light and electron microscopy. The action of these same fixatives and some of their components upon the living cells of the petiolar hairs of Heracleum mantegazzianum is then described. Glutaraldehyde emerged as an excellent fixative for general use from these studies and further tests show that it will stabilize Hecht's threads in plasmolysed onion epidermis against breakage during dehydration. However, the formation of rounded cytoplasmic vacuoles from a pre-existing set of canalicular vacuoles and transformations of the cell membrane and tonoplasts were noted during these studies and none of the fixatives tested prevent the formation of these artefacts.

The Morphology of Vacuolated Cells in the Liver Following Administration of Vitamin A.

1973 ◽  
Vol 31 ◽  
pp. 408-409
Author(s):  
Odell T. Minick ◽  
Hidejiro Yokoo ◽  
Fawzia Batti
Keyword(s):  
Electron Microscopy ◽  
Body Weight ◽  
Vitamin A ◽  
Light And Electron Microscopy ◽  
Liver Cells ◽  
Prominent Feature ◽  
Vascular Space ◽  
Vacuolated Cell ◽  
Vacuolated Cells ◽  
Vacuolated Cytoplasm

Vacuolated cells in the liver of young rats were studied by light and electron microscopy following the administration of vitamin A (200 units per gram of body weight). Their characteristics were compared with similar cells found in untreated animals.In rats given vitamin A, cells with vacuolated cytoplasm were a prominent feature. These cells were found mostly in a perisinusoidal location, although some appeared to be in between liver cells (Fig. 1). Electron microscopy confirmed their location in Disse's space adjacent to the sinusoid and in recesses between liver cells. Some appeared to be bordering the lumen of the sinusoid, but careful observation usually revealed a tenuous endothelial process separating the vacuolated cell from the vascular space. In appropriate sections, fenestrations in the thin endothelial processes were noted (Fig. 2, arrow).

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