scholarly journals Fibrosarcomas in Two Rats Arising from Hepatic Cysts of Cysticercus fasciolaris

1995 ◽  
Vol 32 (4) ◽  
pp. 441-444 ◽  
Author(s):  
M. A. Hanes
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Pleural Cavity ◽  
Sprague Dawley ◽  
Hepatic Cysts ◽  
Multiple Organs ◽  
Sprague Dawley Rats ◽  
Cysticercus Fasciolaris ◽  
Chronic Inflammatory Reaction ◽  
Taenia Taeniaeformis

Two of five male Sprague-Dawley rats with hepatic tapeworm cysts developed large multinodular fibrosarcomas. Fibrosarcomas enveloped tapeworm cysts, invaded the serosa of multiple organs, and extended through the diaphragm into the pleural cavity. Light microscopy, immunohistochemistry, and electron microscopy supported the diagnosis of fibrosarcoma. The parasites were identified as Cysticercus fasciolaris, the larval stage of Taenia taeniaeformis. The development of sarcomas in rats induced by Taenia sp. is thought to be attributable to the chronic inflammatory reaction of the capsule. There are parallels between these and other tumors occurring in mice and cats with suggested chronic inflammatory etiologies.

Ultrastructural investigation of spontaneous pituitary gonadotroph cell adenomas in aging Sprague-Dawley rats

1983 ◽  
Vol 41 ◽  
pp. 702-703
Author(s):  
D. J. McComb ◽  
J. Beri ◽  
F. Zak ◽  
K. Kovacs
Keyword(s):  
Electron Microscopy ◽  
Animal Model ◽  
Epoxy Resin ◽  
Immunoelectron Microscopy ◽  
Tumor Type ◽  
Gonadal Function ◽  
Sprague Dawley ◽  
Male And Female ◽  
Reticulin Fibers ◽  
Sprague Dawley Rats

Gonadotroph cell adenomas of the pituitary are infrequent in human patients and are not invariably associated with altered gonadal function. To date, no animal model of this tumor type exists. Herein, we describe spontaneous gonadotroph cell adenomas in old male and female Sprague-Dawley rats by histology, immunocytology and electron microscopy.The material consisted of the pituitaries of 27 male and 38 female Sprague Dawley rats, all 26 months of age or older, removed at routine autopsy. Sections of formal in-fixed, paraffin-embedded tissue were stained with hematoxylin-phloxine-saffron (HPS), the PAS method and the Gordon-Sweet technique for the demonstration of reticulin fibers. For immunostaining, sections were exposed to anti-rat β-LH, anti-ratβ-TSH, anti-rat PRL, anti-rat GH and anti-rat ACTH 1-39. For electron microscopy, tissue was fixed in 2.5% glutaraldehyde, postfixed in 1% OsO4 and embedded in epoxy-resin. Tissue fixed in 10% formalin, embedded in epoxy resin without osmification, was used for immunoelectron microscopy.

INTERACTIONS BETWEEN CYSTICERCUS FASCIOLARIS AND COMMON RATS

2017 ◽  
Vol 23 (2) ◽  
Author(s):  
SHASHI KIRAN
Keyword(s):  
Granulation Tissue ◽  
Larval Stage ◽  
Histopathological Examination ◽  
Sylvatic Cycle ◽  
Mature Larva ◽  
Hepatic Cysts ◽  
Cysticercus Fasciolaris ◽  
Taenia Taeniaeformis ◽  
Relationship Of ◽  
The Relationship

The objective of this study was to determine the relationship of C. fasciolaris with common rats. Cysticercus fasciolaris is the larval stage of Taenia taeniaeformis . Development of metacestodes ( Cysticercus fasciolaris) occur in the liver of rodents. An urban and a sylvatic cycle occurs. A total of 40 liver specimens of rats were examined. Histopathological examination revealed the presence of multiple hepatic cysts, and the C. fasciolaris larva was surrounded by granulation tissue. Large and separated ûbroblasts in different orientations with some neoplastic changes were seen in advanced hepatic cysticercosis. The bladder involved the larva and large chamber containing the small chamber and opalescent fluid. The scolex of the mature larva is distinctly large, bearing four lateral distinct suckers, a rostellum armed with double, and alternating rows of hooks.

Postjunctional supersensitivity of the rat vas deferens and gap junctions

1976 ◽  
Vol 54 (3) ◽  
pp. 412-416 ◽  
Author(s):  
D. M. Paton ◽  
J. Buckland-Nicks ◽  
A. Johns
Keyword(s):  
Electron Microscopy ◽  
Gap Junctions ◽  
Spontaneous Activity ◽  
Vas Deferens ◽  
Rat Vas Deferens ◽  
Sprague Dawley ◽  
Glutaraldehyde Fixation ◽  
Sprague Dawley Rats

Tissues from the duodenum and vas deferens of Sprague–Dawley rats were examined of the rat vas deferens and gap junctions. Can. J. Physiol. Pharmacol. 54, 412–416. by electron microscopy after glutaraldehyde fixation and postosmication. Gap junctions (nexuses) were readily demonstrated in the duodenum in both control and reserpine treated animals (1.0 mg/kg per day for 7 days). However, gap junctions could not be demonstrated in vas deferens. It is concluded that the postjunctional supersensitivity and spontaneous activity induced by reserpine in vas deferens, does not result from the formation of gap junctions.

Acid Phosphatase Localization in the Cataractous Rat Lens

1977 ◽  
Vol 35 ◽  
pp. 436-437
Author(s):  
N.J. Unakar ◽  
J. Tsui ◽  
J.R. Reddan
Keyword(s):  
Electron Microscopy ◽  
Wound Healing ◽  
Acid Phosphatase ◽  
Tissue Repair ◽  
Lysosomal Enzymes ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Probable Role

Lysosomal enzymes seem to play an important role in wound healing and tissue repair (1,2, and 3). In the present investigation we have shown the existence of acid phosphatase and lysosomes in lenticular tissue and have examined the probable role of this enzyme in the induction of galactose cataract. Experiments were performed on Sprague-Dawley rats (50g) fed for 4 days on a diet consisting of equal proportions (by wt.) of Purina Rat Chow and D-galactose. Animals fed Purina Rat Chow alone served as controls. Acid phosphatase was localized by two separate procedures (4 and 5). Lenses were processed for electron microscopy as previously described (3). Lenticular tissue of both controls (Figs. 1 and 2) and galactose fed animals exhibits the reaction product of acid phosphatase. However, the group fed galactose showed a much stronger reaction product compared to controls.

Structural alterations in early myocardial anoxia

1983 ◽  
Vol 41 ◽  
pp. 792-793
Author(s):  
M. Ashraf ◽  
P.M. Rahamatulla
Keyword(s):  
Electron Microscopy ◽  
Osmium Tetroxide ◽  
Time Interval ◽  
Sprague Dawley ◽  
Structural Alterations ◽  
Sprague Dawley Rats ◽  
Rat Hearts ◽  
Major Coronary Artery ◽  
Temporal Basis ◽  
Isolated Rat

Occlusion of a major coronary artery in a canine results in heterogenous infarct that makes the study of sequential subcellular changes on a temporal basis difficult. In this investigation, global anoxia was induced in isolated rat hearts in order to produce uniform anoxic changes. The hearts removed from Sprague-Dawley rats were perfused with Krebs-Henseleit (KH) medium saturated with 95% O2, 5% CO2 for 30 minutes (control). The ingredients of the anoxic medium were the same except glucose was replaced by mannitol to adjust osmolarity. The anoxic medium was bubbled with 95% N2 and 5% CO2. The perfusion was changed to anoxic medium for 5, 10, 15, 20, 25, and 30 minutes. Six rats were used for each time interval. At the end of experiments, the hearts were perfused with 3% buffered glutaraldehyde, pH 7.3, postfixed with 1% buffered osmium tetroxide for 1 1/2 hours, dehydrated in ethanol and processed for electron microscopy.

Peliosis in Spontaneous Adenomas of the Rat Pituitary. An Electron Microscopic Study

1982 ◽  
Vol 40 ◽  
pp. 352-353
Author(s):  
G. Ilse ◽  
K. Kovacs ◽  
J. Beri ◽  
F. Zak
Keyword(s):  
Electron Microscopy ◽  
Adrenal Cortex ◽  
Microscopic Study ◽  
Sprague Dawley ◽  
Electron Microscopic ◽  
Cell Adenoma ◽  
Prolactin Cell ◽  
Sprague Dawley Rats ◽  
Rat Pituitary ◽  
Long Evans

Lakes of blood are frequently observed in spontaneous prolactin cell adenomas of the rat pituitary. The lesions resemble peliosis, a disorder found mainly in the liver but also in the adrenal cortex. In order to reconstruct the events involved in the formation of these blood lakes, 25 spontaneous prolactin cell adenomas and 1 non-prolactin cell adenoma were harvested from adult female Long-Evans and Sprague-Dawley rats and investigated by electron microscopy.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

Ultrastructural Investigation of Spontaneous Pituitary Adenomas in Aging Sprague-Dawley Rats

1982 ◽  
Vol 40 ◽  
pp. 216-217
Author(s):  
D. J. McComb ◽  
J. Beri ◽  
F. Zak ◽  
K. Kovacs
Keyword(s):  
Microscopic Study ◽  
Pituitary Adenomas ◽  
Wistar Rats ◽  
Microscopic Level ◽  
Sprague Dawley ◽  
Prolactin Cells ◽  
Light Microscopic Level ◽  
Ultrastructural Investigation ◽  
Sprague Dawley Rats ◽  
Long Evans

Investigation of the spontaneous pituitary adenomas in rat have been limited mainly to light microscopic study. Furth et al. (1973) described them as chromophobic, secreting prolactin. Kovacs et al. (1977) in an ul trastructural investigation of adenomas of old female Long-Evans rats, found that they were composed of prolactin cells. Berkvens et al. (1980) using immunocytochemistry at the light microscopic level, demonstrated that some spontaneous tumors of old Wistar rats could contain GH, TSH or ACTH as well as PRL.

Tumor Diagnosis

1982 ◽  
Vol 40 ◽  
pp. 262-265
Author(s):  
Bruce Mackay
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Differential Diagnosis ◽  
Light Microscopy ◽  
Clinical Examination ◽  
Ultrastructural Study ◽  
Diagnostic Procedures ◽  
Specific Diagnosis ◽  
Transmission Electron ◽  
Microscopic Diagnosis

The broadest application of transmission electron microscopy (EM) in diagnostic medicine is the identification of tumors that cannot be classified by routine light microscopy. EM is useful in the evaluation of approximately 10% of human neoplasms, but the extent of its contribution varies considerably. It may provide a specific diagnosis that can not be reached by other means, but in contrast, the information obtained from ultrastructural study of some 10% of tumors does not significantly add to that available from light microscopy. Most cases fall somewhere between these two extremes: EM may correct a light microscopic diagnosis, or serve to narrow a differential diagnosis by excluding some of the possibilities considered by light microscopy. It is particularly important to correlate the EM findings with data from light microscopy, clinical examination, and other diagnostic procedures.

Early Development of Drug-Induced Hepatic Necrosis

1971 ◽  
Vol 29 ◽  
pp. 576-577
Author(s):  
F. G. Zaki ◽  
E. Detzi ◽  
C. H. Keysser
Keyword(s):  
Early Development ◽  
Hepatic Necrosis ◽  
Screening Tests ◽  
Dense Matrix ◽  
Sprague Dawley ◽  
Electron Microscopic ◽  
Drug Induced ◽  
Hepatocellular Damage ◽  
Sprague Dawley Rats ◽  
Microscopic Studies

This study represents the first in a series of investigations carried out to elucidate the mechanism(s) of early hepatocellular damage induced by drugs and other related compounds. During screening tests of CNS-active compounds in rats, it has been found that daily oral administration of one of these compounds at a dose level of 40 mg. per kg. of body weight induced diffuse massive hepatic necrosis within 7 weeks in Charles River Sprague Dawley rats of both sexes. Partial hepatectomy enhanced the development of this peculiar type of necrosis (3 weeks instead of 7) while treatment with phenobarbital prior to the administration of the drug delayed the appearance of necrosis but did not reduce its severity.Electron microscopic studies revealed that early development of this liver injury (2 days after the administration of the drug) appeared in the form of small dark osmiophilic vesicles located around the bile canaliculi of all hepatocytes (Fig. 1). These structures differed from the regular microbodies or the pericanalicular multivesicular bodies. They first appeared regularly rounded with electron dense matrix bound with a single membrane. After one week on the drug, these vesicles appeared vacuolated and resembled autophagosomes which soon developed whorls of concentric lamellae or cisterns characteristic of lysosomes (Fig. 2). These lysosomes were found, later on, scattered all over the hepatocytes.

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