Structural alterations in early myocardial anoxia
Occlusion of a major coronary artery in a canine results in heterogenous infarct that makes the study of sequential subcellular changes on a temporal basis difficult. In this investigation, global anoxia was induced in isolated rat hearts in order to produce uniform anoxic changes. The hearts removed from Sprague-Dawley rats were perfused with Krebs-Henseleit (KH) medium saturated with 95% O2, 5% CO2 for 30 minutes (control). The ingredients of the anoxic medium were the same except glucose was replaced by mannitol to adjust osmolarity. The anoxic medium was bubbled with 95% N2 and 5% CO2. The perfusion was changed to anoxic medium for 5, 10, 15, 20, 25, and 30 minutes. Six rats were used for each time interval. At the end of experiments, the hearts were perfused with 3% buffered glutaraldehyde, pH 7.3, postfixed with 1% buffered osmium tetroxide for 1 1/2 hours, dehydrated in ethanol and processed for electron microscopy.