Acid Phosphatase Localization in the Cataractous Rat Lens
Lysosomal enzymes seem to play an important role in wound healing and tissue repair (1,2, and 3). In the present investigation we have shown the existence of acid phosphatase and lysosomes in lenticular tissue and have examined the probable role of this enzyme in the induction of galactose cataract. Experiments were performed on Sprague-Dawley rats (50g) fed for 4 days on a diet consisting of equal proportions (by wt.) of Purina Rat Chow and D-galactose. Animals fed Purina Rat Chow alone served as controls. Acid phosphatase was localized by two separate procedures (4 and 5). Lenses were processed for electron microscopy as previously described (3). Lenticular tissue of both controls (Figs. 1 and 2) and galactose fed animals exhibits the reaction product of acid phosphatase. However, the group fed galactose showed a much stronger reaction product compared to controls.