A highly efficient multi-step methodology for the quantification of micro-(bio)plastics in sludge

2020 ◽  
pp. 0734242X2097409
Author(s):  
Federica Ruggero ◽  
Alexandra E. Porter ◽  
Nikolaos Voulvoulis ◽  
Emiliano Carretti ◽  
Tommaso Lotti ◽  
...  
Keyword(s):  
Organic Waste ◽  
Degradation Process ◽  
Organic Fertilizers ◽  
Critical Conditions ◽  
Organic Fraction ◽  
Initial Oxidation ◽  
Threshold Size ◽  
End Products ◽  
Biological Treatments ◽  
Pre Treatment

The present study develops a multi-step methodology for identification and quantification of microplastics and micro-bioplastics (together called in the current work micro-(bio)plastics) in sludge. In previous studies, different methods for the extraction of microplastics were devised for traditional plastics, while the current research tested the methodology on starch-based micro-bioplastics of 0.1–2 mm size. Compostable bioplastics are expected to enter the anaerobic or aerobic biological treatments that lead to end-products applicable in agriculture; some critical conditions of treatments (e.g. low temperature and moisture) can slow down the degradation process and be responsible for the presence of microplastics in the end-product. The methodology consists of an initial oxidation step, with hydrogen peroxide 35% concentrated to clear the sludge and remove the organic fraction, followed by a combination of flotation with sodium chloride and observation of the residues under a fluorescence microscope using a green filter. The workflow revealed an efficacy of removal from 94% to 100% and from 92% to 96% for plastic fragments, 0.5–2 mm and 0.1–0.5 mm size, respectively. The methodology was then applied to samples of food waste pulp harvested after a shredding pre-treatment in an anaerobic digestion (AD) plant in Italy, where polyethylene, starch-based Mater-Bi® and cellophane microplastics were recovered in amounts of 9 ± 1.3/10 g <2 mm and 4.8 ± 1.2/10 g ⩾2 mm. The study highlights the need to lower the threshold size for the quantification of plastics in organic fertilizers, which is currently set by legislations at 2 mm, by improving the background knowledge about the fate of the micro-(bio)plastics in biological treatments for the organic waste.

Effect of addition of anaerobic fermented OFMSW (organic fraction of municipal solid waste) on biological nutrient removal (BNR) process: preliminary results

1998 ◽  
Vol 38 (1) ◽  
pp. 327-334 ◽  
Author(s):  
P. Pavan ◽  
P. Battistoni ◽  
P. Traverso ◽  
A. Musacco ◽  
F. Cecchi
Keyword(s):  
Nutrient Removal ◽  
Fermentation Process ◽  
Organic Waste ◽  
Anaerobic Fermentation ◽  
Pilot Scale ◽  
Biological Nutrient Removal ◽  
Organic Fraction ◽  
P Release ◽  
Pure Methanol ◽  
P Removal

The paper presents results coming from experiments on pilot scale plants about the possibility to integrate the organic waste and wastewater treatment cycles, using the light organic fraction produced via anaerobic fermentation of OFMSW as RBCOD source for BNR processes. The effluent from the anaerobic fermentation process, with an average content of 20 g/l of VFA+ lactic acid was added to wastewater to be treated in order to increase RBCOD content of about 60-70 mg/l. The results obtained in the BNR process through the addition of the effluent from the fermentation unit are presented. Significant increase of denitrification rate was obtained: 0.06 KgN-NO3/KgVSS d were denitrified in the best operative conditions studied. -Vmax shows values close to those typical of the pure methanol addition (about 0.3 KgN-NO3/KgVSS d). A considerable P release (35%) was observed in the anaerobic step of the BNR process, even if not yet a completely developed P removal process.

scholarly journals Conversion of Spent Coffee and Donuts by Black Soldier Fly (Hermetia illucens) Larvae into Potential Resources for Animal and Plant Farming

Insects ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 332
Author(s):  
Hayden Fischer ◽  
Nicholas Romano ◽  
Amit Kumar Sinha
Keyword(s):  
Organic Waste ◽  
Glycogen Content ◽  
Organic Fertilizer ◽  
Organic Fertilizers ◽  
Black Soldier Fly ◽  
Hermetia Illucens ◽  
Hermetia Illucens Larvae ◽  
Nitrogen Phosphorus ◽  
Dough Stage ◽  
Plant Farming

Nutritionally unbalanced organic waste can be converted into potential resources for animal and plant farming by culturing black soldier fly (Hermetia illucens) larvae (BSFL) and prepupae (BSFP). BSFL and BSFP are rich sources of protein and lipids, while the leftover excrement called “frass” can be used as an organic fertilizer. Using readily available resources, BSFL were cultured on spent coffee, donut dough or an equal blend for 35 days. Survival, productivity, daily pupation and biochemical composition of BSFL and BSFP were measured along with the nitrogen-phosphorus-potassium values of the frass. Survival was highest in the blend compared (81%) to spent coffee (45%) or dough (24%); however, BSFL and BSFP were significantly longer and heavier from dough. Stage and food significantly influenced the protein, lipid and glycogen content of the BSFL and BSFP, which tended to be higher in the latter. While fatty acids were often significantly higher in BSFL fed spent coffee, the amino acid composition of BSFL was generally higher in dough. Frass from the blend had significantly highest nitrogen content, while potassium and phosphorus were significantly higher and lower from spent coffee, respectively. Although coffee and donut dough were suboptimal substrates for BSFL, a blend of these produced BSFL and frass that were nutritionally comparable to soybean meal and many organic fertilizers, respectively.

scholarly journals Adipocyte death triggers a pro-inflammatory response and induces metabolic activation of resident macrophages

2021 ◽  
Vol 12 (6) ◽  
Author(s):  
Andreas Lindhorst ◽  
Nora Raulien ◽  
Peter Wieghofer ◽  
Jens Eilers ◽  
Fabio M. V. Rossi ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Metabolic Activation ◽  
Degradation Process ◽  
Critical Threshold ◽  
Low Grade ◽  
Blood Monocytes ◽  
Threshold Size ◽  
Laser Injury ◽  
Reporter Mice

AbstractA chronic low-grade inflammation within adipose tissue (AT) seems to be the link between obesity and some of its associated diseases. One hallmark of this AT inflammation is the accumulation of AT macrophages (ATMs) around dead or dying adipocytes, forming so-called crown-like structures (CLS). To investigate the dynamics of CLS and their direct impact on the activation state of ATMs, we established a laser injury model to deplete individual adipocytes in living AT from double reporter mice (GFP-labeled ATMs and tdTomato-labeled adipocytes). Hence, we were able to detect early ATM-adipocyte interactions by live imaging and to determine a precise timeline for CLS formation after adipocyte death. Further, our data indicate metabolic activation and increased lipid metabolism in ATMs upon forming CLS. Most importantly, adipocyte death, even in lean animals under homeostatic conditions, leads to a locally confined inflammation, which is in sharp contrast to other tissues. We identified cell size as cause for the described pro-inflammatory response, as the size of adipocytes is above a critical threshold size for efferocytosis, a process for anti-inflammatory removal of dead cells during tissue homeostasis. Finally, experiments on parabiotic mice verified that adipocyte death leads to a pro-inflammatory response of resident ATMs in vivo, without significant recruitment of blood monocytes. Our data indicate that adipocyte death triggers a unique degradation process and locally induces a metabolically activated ATM phenotype that is globally observed with obesity.

scholarly journals Bioelectricity production from tofu wastewater using microbial fuel cells with microalgae Spirulina sp as catholyte

2020 ◽  
Vol 202 ◽  
pp. 08007
Author(s):  
Wahyu Zuli Pratiwi ◽  
Hadiyanto Hadiyanto ◽  
Purwanto Purwanto ◽  
Muthi’ah Nur Fadlilah
Keyword(s):  
Wastewater Treatment ◽  
Fuel Cells ◽  
Microbial Fuel Cells ◽  
Organic Waste ◽  
Oxygen Demand ◽  
Salt Bridge ◽  
Cod Removal ◽  
Biofuel Production ◽  
Pre Treatment ◽  
Made In

Microalgae-Microbial Fuel Cells (MMFCs) are very popular to be used to treat organic waste. MMFCs can function as an energy-producing wastewater pre-treatment system. Wastewater can provide an adequate supply of nutrients, support the large capacity of biofuel production, and can be integrated with existing wastewater treatment infrastructure. The reduced content of Chemical Oxygen Demand (COD) is one way to measure the efficiency of wastewater treatment. MMFCs reactors are made in the form of two chambers (anode and cathode) both of which are connected by a salt bridge. Tofu wastewater as an anode and Spirulina sp as a cathode. To improve MFCs performance which is to obtain maximum COD removal and electricity generation, nutrient NaHCO3 as the nutrient carbon source for Spirulina sp was varied. The system running phase on 12 days. The results were Spirulina sp treated with MFCs technology has better growth than non-MFCs. The MMFC generated a maximum power density of 21.728 mW/cm2 and achieved 57.37% COD removal. These results showed that the combined process was effective in treating tofu wastewater.

Effects of pre-treatment technologies on quantity and quality of source-sorted municipal organic waste for biogas recovery

2007 ◽  
Vol 27 (3) ◽  
pp. 398-405 ◽  
Author(s):  
Trine Lund Hansen ◽  
Jes la Cour Jansen ◽  
Åsa Davidsson ◽  
Thomas Højlund Christensen
Keyword(s):  
Organic Waste ◽  
Treatment Technologies ◽  
Pre Treatment ◽  
Biogas Recovery

Investigation and assessment of sludge pre-treatment processes

2004 ◽  
Vol 49 (10) ◽  
pp. 97-104 ◽  
Author(s):  
J.A. Müller ◽  
A. Winter ◽  
G. Strünkmann
Keyword(s):  
Fine Particles ◽  
Degradation Process ◽  
Solid Content ◽  
Ozone Treatment ◽  
Treatment Processes ◽  
Sludge Properties ◽  
Pre Treatment ◽  
The Cost ◽  
Degree Of Degradation ◽  
Cell Disintegration

The pre-treatment of sludges by disintegration will result in a number of changes in sludge properties. Floc destruction as well as cell disintegration will occur. This leads to an increase of soluble substances and fine particles. Furthermore, biochemical reactions may appear during or immediately after disintegration. The influence of disintegration of excess sludge on anaerobic digestion was studied in full scale. A stirred ball mill, an ultrasound disintegrator, a lysate centrifuge and ozone treatment were used. The results of the degradation process were compared to a reference system without pre-treatment. An enhancement of the degree of degradation of 7.4% to 20% was observed. The pollution of sludge water as well as the dewatering properties of the digested sludge were investigated. COD and ammonia in the sludge water were increased and a higher polymer demand was observed while the solid content after dewatering stayed almost unchanged. Based on these results the cost effectiveness has been assessed taking into account different conditions (size of WWTP, cost for disposal, etc.). Capital and energy costs are the main factors while the decrease in disposal costs due to the reduced amount of sludge is the main profit factor.

scholarly journals Kinetics of Anaerobic Digestion of Dairy Fat Waste with Saponification Pre-Treatment

2019 ◽  
Vol 13 (2) ◽  
pp. 112 ◽  
Author(s):  
Rifki Wahyu Kurnianto ◽  
Wiratni Budhijanto ◽  
Rochim Bakti Cahyono
Keyword(s):  
Anaerobic Digestion ◽  
Lipid Content ◽  
Alternative Energy ◽  
Organic Waste ◽  
High Lipid Content ◽  
Dairy Fat ◽  
High Lipid ◽  
Pre Treatment ◽  
Kinetics Of ◽  
Floating Layer

Anaerobic digestion has been an attractive field of research in the era of energy crisis. Biogas, which is the product of anaerobic digestion, provides alternative energy, while at the same time it also prevents pollution due to organic waste accumulation. Among various organic wastes, dairy fat waste is a potential substrate for anaerobic digestion. Fat waste has high theoretical biogas potential because of its high lipid content. However, anaerobic digestion of organic waste with high lipid content is quite challenging. The main obstacle in anaerobic digestion of fat waste is its tendency to form insoluble floating layer on top of the liquid phase. This phenomenon hinders the access of hydrolytic bacteria to the substrate. Saponification is one of the methods to increase the solubility of the floating layer and hence to improve the availability of substrate for the bacteria. Saponification changes the lipid content into soap which has both polar and non-polar functional groups and the polar side will increase the solubility of the substrate in water. This study evaluated the effect of different dosage of base added as the reactant during saponification pre-treatment on the productivity of anaerobic digestion of dairy fat waste. The kinetics of the anaerobic digestion process was analyzed by mean of mathematical model. The variations of the alkaline dosages studied for saponification pre-treatment were 0.04 mol base/g sCOD; 0.02 mol base/g sCOD; and no pre-treatment for control reactor. This study proved that saponification increased the solubility of dairy fat waste. This result was confirmed by the hydrolysis constant value (kH) of 0.00782/day for reactor with saponification, which was twenty times of magnitude higher than the kH value of 0.00032/day in the reactor without saponification. However, the exposure to high pH during the saponification pre-treatment might somewhat inhibit indigenous acidogenic bacteria in the waste which results in lower methane yield in the reactors with saponification to be compared to the control reactor. A B S T R A KPeruraian anaerobik merupakan salah satu bidang riset yang sangat menarik perhatian dalam era krisis energi. Biogas tidak hanya menyediakan energi alternatif, tetapi juga dapat mencegah pencemaran akibat limbah organik. Limbah lemak susu adalah substrat yang potensial untuk proses peruraian anaerobik karena memiliki potensi biogas teoritis yang tinggi akibat kandungan lemaknya yang tinggi. Namun, peruraian anaerobik dari limbah organik dengan kandungan lemak yang tinggi memiliki tantangan tersendiri. Hambatan utama dalam peruraian anaerobik dari limbah lemak susu adalah kecenderungan untuk membentuk lapisan padatan yang tidak larut dan mengapung di bagian atas fase cair. Fenomena ini menghambat akses bakteri hidrolisis terhadap substrat. Saponifikasi adalah salah satu cara untuk meningkatkan kelarutan lapisan padatan tersebut, sehingga meningkatkan ketersediaan substrat untuk bakteri. Saponifikasi akan mengubah kandungan lemak menjadi sabun yang memiliki gugus fungsi polar maupun non-polar. Gugus fungsi yang bersifat polar akan meningkatkan kelarutan substrat dalam air. Studi ini mengevaluasi pengaruh dari berbagai dosis larutan basa yang ditambahkan sebagai reaktan selama perlakuan awal saponifikasi terhadap peruraian anaerobik limbah lemak susu. Kinetika proses peruraian anaerobik dianalisis dengan menggunakan model matematika. Variasi dosis yang diamati pengaruhnya untuk perlakuan awal saponifikasi adalah 0,04 mol basa/g sCOD; 0,02 mol basa/g sCOD; dan nol (tanpa perlakuan awal saponifikasi). Dari penelitian ini, terbukti bahwa saponifikasi berhasil meningkatkan kelarutan limbah lemak susu dan juga ditunjukkan oleh nilai konstanta hidrolisis (kH) 0,00782/hari lebih tinggi dua puluh kali lipat dibandingkan dengan nilai kH 0,00032/hari pada reaktor tanpa saponifikasi. Akan tetapi, penelitian ini juga mengindikasikan bahwa bakteri asidogenik bawaan substrat terhambat kinerjanya oleh paparan pH yang tinggi selama perlakuan awal saponifikasi berlangsung sehingga hasil gas metan yang diperoleh lebih rendah daripada reaktor kontrol.

scholarly journals MULTIFUNCTIONAL BIOLOGICAL PRODUCTS FOR ORGANIC FARMING BASED ON ORGANIC WASTE AND RAW MATERIAL PROCESSING

2021 ◽  
pp. 36-42
Author(s):  
О.А. Оленин ◽  
С.Н. Зудилин
Keyword(s):  
Winter Wheat ◽  
Organic Farming ◽  
Organic Waste ◽  
Raw Materials ◽  
Raw Material ◽  
Organic Fertilizers ◽  
Mineral Fertilizers ◽  
Plant Origin ◽  
Biological Product ◽  
Biological Products

Развитие органического земледелия, в первую очередь, зависит от наличия органических удобрений и биопрепаратов. Цель исследований – на основе утилизации органических отходов и сырья разработать полифункциональные биопрепараты из компонентов животного и растительного происхождения и изучить их влияние на показатели агрофитоценозов и урожайность полевых и садовых культур. В работе использованы результаты исследований по разработке полифункциональных биопрепаратов, а также их эффективности на опытном поле Самарского ГАУ в 2017-2019 гг. В результате была разработана ассортиментная линейка биопрепаратов «АгроТоник» с функциями удобрения и биостимулятора роста: «Цветовод», «Садовод», «Овощевод», «Полевод», «Лесовод» и «Универсальный». «АгроТоник», в отличие от многих органических и минеральных удобрений, содержит все необходимые для растений компоненты: макро-, мезо- и микроэлементы в легкодоступной форме, микроорганизмы, стимуляторы роста – биоактивные фитогормоны, аминокислоты растительного происхождения, витамины, комплекс целлюлозолитических ферментов, почвенные антибиотики, гуминовые вещества, биоприлипатель. Многокомпонентный биопрепарат с функциями удобрения, фунгицида и бактерицида снижал пораженность растений озимой пшеницы на 16,7-27,1 %, по отношению к контролю, и на 17,4-22,6 %, по сравнению с минеральными удобрениями. Биопрепарат повышал урожайность озимой пшеницы на 7,7-25,4 % по сравнению с контролем, тогда как применение пестицида только на 5,3-11,5 %, при стоимости однократно внесенного гербицида в среднем 500,00 руб./га, а биопрепарата – 300,00 руб./га при двукратной обработке. The development of organic farming primarily depends on the availability of organic fertilizers and biological products. The goal of the research is to develop multifunctional biological products from components of animal and plant origin based on the utilization of organic waste and raw materials and to study their impact on the indicators of agrophytocenoses and the yield of field and horticultural crops. The work uses the results of research on the development of multifunctional biological products, as well as their effectiveness in the experimental field of the Samara State Agrarian University in 2017-2019. As a result, the line of "AgroTonik" biological products with the functions of fertilizer and the growth biostimulator was developed: «Tsvetovod», «Sadovod», «Ovoshchevod», «Polevod», «Lesovod» and «Universal'niy». "AgroTonik", unlike many organic and mineral fertilizers, contains all the components necessary for plants: macro -, meso- and microelements in an easily available form, microorganisms, growth stimulators – bioactive phytohormones, amino acids of plant origin, vitamins, a complex of cellulosolytic enzymes, soil antibiotics, humic substances, bio-sticking agent. The multicomponent biological product with the functions of fertilizer, fungicide and bactericide reduced the affection of winter wheat plants by 16.7-27.1 % concerning the control, and by 17.4-22.6 %, in comparison with mineral fertilizers. The biological product increased the yield of winter wheat by 7.7-25.4% compared to the control, while the use of a pesticide only by 5.3-11.5 %, with the cost of a single herbicide on average 500 rubles/ha, and a biological product – 300 rubles/ha with double treatment.

scholarly journals ANALISA PEMANFAATAN KOMPOS SEBAGAI MEDIA BIOCOVER DI TEMPAT PEMROSESAN AKHIR SAMPAH: Studi Kasus Kota Depok, Jawa Barat

REAKTOR ◽  
2014 ◽  
Vol 15 (2) ◽  
pp. 117
Author(s):  
Gabriel Andari Kristanto ◽  
Dwi Rahayu ◽  
Evy Novita
Keyword(s):  
Methane Emission ◽  
Urban Areas ◽  
Organic Waste ◽  
Good Alternative ◽  
Waste Processing ◽  
Maturation Process ◽  
Compaction Rate ◽  
Process Study ◽  
Mature Compost ◽  
Pre Treatment

High percentage of organic waste in urban areas makes composting as good alternative in waste processing. However, composting has not been effectively used and the result is not optimal. Previous studies found compost can be used as biocover media to reduce methane emission landfill. The objective of the study is to identify the characteristic of produced at temporary waste unit Depok, West Java and the effect of compaction toward the amount of methane removal in the landfill. Before applied as biocover media, compost was characterized followed by maturation process. Study on methane removal efficiency was conducted as batches in four reactors for 35 days with compaction rate varying from 750, 800, 850 dan 900 kg/m3. Composting media used in the research had 80 cm thickness with artificial gas consisting of 50% CH4 dan 50% CO2. Initially, compost did not meet criteria as mature compost based on SNI 19-7030-2004, and as media biocover hence a pre-treatment was needed to improve the quality compost to meet the requirements. Compaction rate of 900 kg/m3has highest elimination capacity of methane i.e. 98,31%±3,6% and 13,98±4,32 g/m3/hour.

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