Gelatin/poly(vinyl alcohol) based hydrogel film – A potential biomaterial for wound dressing: Experimental design and optimization followed by rotatable central composite design

2021 ◽  
pp. 088532822199226
Author(s):  
Patricia Hubner ◽  
Nilson Romeu Marcilio ◽  
Isabel Cristina Tessaro
Keyword(s):  
Experimental Design ◽  
Central Composite Design ◽  
Vinyl Alcohol ◽  
Biomedical Applications ◽  
Poly Vinyl Alcohol ◽  
Swelling Kinetics ◽  
Mechanical Resistance ◽  
Hydrogel Film ◽  
Central Composite ◽  
Hydrogel Films

The development of hydrogel films for biomedical applications is interesting due to their characteristics. Hydrogel films based on gelatin and poly(vinyl alcohol) (PVA) are developed and characterized using a rotatable central composite design. The optimized hydrogel film is obtained by the function desirability of the Statistica® software and is also characterized by swelling kinetics, oxygen permeability, adhesiveness, TGA, DSC, and XRD. The results of the experimental design show that gelatin and PVA concentrations have a significant influence on the response variables, and the exposure doses to UV light show no significant effect. The optimized hydrogel film is elastic, presents good mechanical resistance and swelling capacity in water and exudate solution, is permeable to oxygen, and is capable of adjusting itself and maintains contact close to the skin. In this way, considering all the properties evaluated, the optimized film has characteristics suitable for biomedical applications as wound dressings.

scholarly journals Optimization of electrospinning process of poly(vinyl alcohol) via response surface methodology (RSM) based on the central composite design

2014 ◽  
Vol 3 (3) ◽  
pp. 175-182 ◽  
Author(s):  
Mina Yazdanpanah ◽  
MohammadReza Khanmohammadi ◽  
Ruhollah Mehdinavaz Aghdam ◽  
Keyvan Shabani ◽  
Masoud Rajabi
Keyword(s):  
Response Surface Methodology ◽  
Central Composite Design ◽  
Response Surface ◽  
Vinyl Alcohol ◽  
Electrospinning Process ◽  
Poly Vinyl Alcohol ◽  
Central Composite

Semi-interpenetrating polymeric networks based on poly(dimethylsiloxane)-chitosan-poly(vinyl alcohol) crosslinked with genipin with possible use in biomedical applications

2021 ◽  
Vol 56 (9) ◽  
pp. 5936-5955
Author(s):  
J. A. Benítez-Martínez ◽  
I. M. Garnica-Palafox ◽  
G. Vázquez-Victorio ◽  
M. Hautefeuille ◽  
F. M. Sánchez-Arévalo
Keyword(s):  
Vinyl Alcohol ◽  
Biomedical Applications ◽  
Poly Vinyl Alcohol ◽  
Polymeric Networks ◽  
Interpenetrating Polymeric Networks

Proton conduction in new polymer hydrogel films consisting of crosslinking partially phosphorylated poly(vinyl alcohol)s

1999 ◽  
Vol 1 (11) ◽  
pp. 2749-2753 ◽  
Author(s):  
Masahiro Suzuki ◽  
Takahide Yoshida ◽  
Satoshi Kobayashi ◽  
Toshiki Koyama ◽  
Mutsumi Kimura ◽  
...  
Keyword(s):  
Vinyl Alcohol ◽  
Proton Conduction ◽  
Poly Vinyl Alcohol ◽  
Polymer Hydrogel ◽  
Hydrogel Films

Modeling and optimization of antibacterial activity of the chitosan-based hydrogel films using central composite design

2016 ◽  
Vol 104 (10) ◽  
pp. 2544-2553 ◽  
Author(s):  
Behnaz Lahooti ◽  
Mohammad Khorram ◽  
Gholamreza Karimi ◽  
Aliakbar Mohammadi ◽  
Amir Emami
Keyword(s):  
Antibacterial Activity ◽  
Central Composite Design ◽  
Modeling And Optimization ◽  
Central Composite ◽  
Hydrogel Films

Preparation of Antimicrobial Aminoclays/Poly(vinyl alcohol) Nanocomposite Hydrogel Films by Freeze-Thawing Method

2019 ◽  
Vol 27 (6) ◽  
pp. 523-525
Author(s):  
Vu Khac Hoang Bui ◽  
Duckshin Park ◽  
Minh Kim Nguyen ◽  
Ju-Young Moon ◽  
Young-Chul Lee
Keyword(s):  
Vinyl Alcohol ◽  
Poly Vinyl Alcohol ◽  
Nanocomposite Hydrogel ◽  
Hydrogel Films

Optimization of Linoleic Acid Monoepoxidation Condition from Malaysian Jatropha curcas Using Central Composite Design (CCD)

2015 ◽  
Vol 754-755 ◽  
pp. 1107-1112
Author(s):  
Rozaini Abdullah ◽  
Jumat Salimon ◽  
Anis Atikah Ahmad
Keyword(s):  
Linoleic Acid ◽  
Reaction Time ◽  
Experimental Design ◽  
Central Composite Design ◽  
Reaction Temperature ◽  
Jatropha Curcas ◽  
Catalyst Loading ◽  
Time Saving ◽  
Independent Variable ◽  
Central Composite

The aim of this study was to optimize the monoepoxidation process of linoleic acid obtained from Malaysian Jatropha curcas oil using central composite design (CCD). There were four independent variable factors had been studied which involved reaction temperature (X1), reaction time (X2), catalyst loading (X3) and H2O2 concentration (X4). Thirty experiments were carried out based on the experimental design responses obtained. The results showed that the optimum condition was obtained at catalyst loading of 0.11% (w/w) methyltrioxorhernium (VII) (MTO), H2O2 mole of 99%, reaction temperature of 58.41oC for 5 hours. The central composite design was proven to be simpler method, time saving and required less samples compared to the conventional method.

scholarly journals In Situ Preparation and Properties of Poly(vinyl alcohol)/Carboxymethyl Chitosan/Cyanidin Hydrogel Films

2019 ◽  
Vol 2019 ◽  
pp. 1-8
Author(s):  
Xiaojuan Zhang ◽  
Wenbin Cai ◽  
Lingyun Hao ◽  
Wei Zhang
Keyword(s):  
Vinyl Alcohol ◽  
Encapsulation Efficiency ◽  
Umbilical Vein ◽  
Antibacterial Activities ◽  
Carboxymethyl Chitosan ◽  
Poly Vinyl Alcohol ◽  
In Situ Preparation ◽  
Umbilical Vein Endothelial Cells ◽  
Hydrogel Films

This study synthesized poly(vinyl alcohol)/carboxymethyl chitosan/cyanidin (PVA/CMCS/CY) hydrogel films. First, PVA and CMCS were used to synthesize hydrogel films by ultraviolet irradiation. Meanwhile, CY was in situ combined into the hydrogel films through the electrostatic attraction between CMCS and CY. Next, the products were analyzed using Fourier-transform infrared spectroscopy, scanning electron microscopy, contact angle test, swelling analysis, and mechanical property test. The results revealed that compared with PVA/CMCS hydrogel films, the PVA/CMCS/CY hydrogel films had an interporous structure and good swelling and mechanical properties. Moreover, the drug release experiments demonstrated that the PVA/CMCS/CY hydrogel films had a CY encapsulation efficiency of 33.5% with a sustained CY release of up to 60 h. Furthermore, the examined antibacterial activities against Staphylococcus aureus and Escherichia coli showed that the porous PVA/CMCS/CY hydrogel films exhibited a certain inhibition. Cell viability experiments demonstrated that the PVA/CMCS/CY films displayed no obvious cytotoxicity to human umbilical vein endothelial cells.

Optimization of Critical Medium Components for the Expression of Recombinant Human Transferrin in Insect Cells Baculovirus System

2012 ◽  
Author(s):  
Clarence M. Ongkudon ◽  
Badarulhisam Abdul Rahman ◽  
Azila Abd. Aziz
Keyword(s):  
Response Surface Methodology ◽  
Experimental Design ◽  
Central Composite Design ◽  
Response Surface ◽  
Insect Cells ◽  
Expression System ◽  
Proliferating Cells ◽  
Human Transferrin ◽  
Lipid Mixtures ◽  
Central Composite

Transferin manusia (hTf) memainkan peranan yang penting dalam fungsi bakteriostatik dan pengangkutan ferum dari bahagian penyimpanan ke sel–sel yang membiak melalui proses endositosis janaan reseptor. Sistem ekspresi bakulovirus sel serangga telah dipakai secara meluas sebagai sistem alternatif dalam penghasilan Transferin manusia rekombinan (rhTf). Kajian ini ditumpukan ke atas pengoptimuman glutamina, glukosa dan campuran lipid 1000x yang dapat meningkatkan penghasilan rhTf. Reka bentuk eksperimen yang melibatkan 17 eksperimen reka bentuk komposit berpusat (CCD) telah digunakan dan hasil kajian dianalisis oleh Statistika (Statsoft v. 5.0). Metodologi permukaan tindak balas (RSM) telah mengenalpasti nilai optimum parameterparameter yang dikaji iaitu glutamina=2211.20 mg/L, glukosa=1291.95 mg/L, dan campuran lipid 1000x=0.64 %v/v. Hasil optimasi menunjukkan peningkatan hasil rhTf sebanyak tiga kali ganda, iaitu daripada 19.89 μg/ml kepada 65.12 μg/ml. Kata kunci: Transferin manusia; bakulovirus sel serangga; reka bentuk eksperimen; reka bentuk komposit berpusat; metodologi permukaan tindak balas Human Transferrin (hTf) plays a big role in providing bacteriostatic functions as well as to transport iron from the storage part to all proliferating cells by receptor mediated endocytosis. Insect cells baculovirus expression system has been widely used as an alternative expression system for the production of recombinant human Transferrin (rhTf). This work focused mainly on the optimization of glutamine, glucose and lipid mixtures 1000x to increase rhTf yield. An experimental design involving 17 central composite design (CCD) experiments was employed and results were analyzed by Statistica (Statsoft v. 5.0). The response surface methodology (RSM) had identified the optimum values where glutamine=2211.20 mg/L, glucose=1291.95 mg/L, and lipid mixtures 1000x=0.64 %v/v. Using the optimized parameters, the studies demonstrated an increase in the rhTf yield by three–fold from 19.89 μg/ml to 65.12 μg/ml. Key words: Human transferrin; insect cells baculovirus; experimental design; central composite design; response surface methodology

scholarly journals Poly(vinyl alcohol)-Based Biofilms Plasticized with Polyols and Colored with Pigments Extracted from Tomato By-Products

Polymers ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 532 ◽  
Author(s):  
Laura Mitrea ◽  
Lavinia-Florina Călinoiu ◽  
Gheorghe-Adrian Martău ◽  
Katalin Szabo ◽  
Bernadette-Emoke Teleky ◽  
...  
Keyword(s):  
Vinyl Alcohol ◽  
Flow Behavior ◽  
Poly Vinyl Alcohol ◽  
Mechanical Resistance ◽  
Physicochemical Features ◽  
Synthetic Colorants ◽  
Coloring Agents ◽  
Orange Color ◽  
By Products ◽  
Β Carotene

In the current work the physicochemical features of poly(vinyl alcohol) (PVOH) biofilms, enriched with eco-friendly polyols and with carotenoid-rich extracts, were investigated. The polyols, such as glycerol (Gly), 1,3-propanediol (PDO), and 2,3-butanediol (BDO) were used as plasticizers and the tomato-based pigments (TP) as coloring agents. The outcomes showed that β-carotene was the major carotenoid in the TP (1.605 mg β-carotene/100 DW), which imprinted the orange color to the biofilms. The flow behavior indicated that with the increase of shear rate the viscosity of biofilm solutions also increased until 50 s−1, reaching values at 37 °C of approximately 9 ± 0.5 mPa·s for PVOH, and for PVOH+TP, 14 ± 0.5 mPa·s in combination with Gly, PDO, and BDO. The weight, thickness, and density of samples increased with the addition of polyols and TP. Biofilms with TP had lower transparency values compared with control biofilms (without vegetal pigments). The presence of BDO, especially, but also of PDO and glycerol in biofilms created strong bonds within the PVOH matrix by increasing their mechanical resistance. The novelty of the present approach relies on the replacement of synthetic colorants with natural pigments derived from agro-industrial by-products, and the use of a combination of biodegradable polymers and polyols, as an integrated solution for packaging application in the bioplastic industry.

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