The effect of composite PHB coating on the biological properties of a magnesium based alloy

2021 ◽  
pp. 088532822199804
Author(s):  
Quan Liang ◽  
Shuping Ge ◽  
Chenyu Liu ◽  
Xuejun Quan ◽  
Binbin Tan ◽  
...  
Keyword(s):  
Magnesium Alloys ◽  
Anodic Oxidation ◽  
Potential Surface ◽  
Blood Compatibility ◽  
Umbilical Vein ◽  
Antibacterial Properties ◽  
Copper Film ◽  
Biological Properties ◽  
Vascular Stents ◽  
Umbilical Vein Endothelial Cells

Magnesium alloys have been widely investigated as biodegradable cardiovascular temporal implants due to their better mechanical properties and biocompatibility, but the rapid degradation limited its application. In this study, the anodic oxidation-Cu structure was used to improve the adhesive strength and stability between poly-β-hydroxybutyrate (PHB) and magnesium alloys, and the effects of anodic oxidation magnesium alloys with copper film and PHB film (MACP) on human umbilical vein endothelial cells (HUVECs), blood compatibility and antibacterial properties were investigated in this research. As the result, the MACP structure had a stable structure and better corrosion resistance, and significant antibacterial properties. The coating would not affect the original excellent biocompatibility of the magnesium alloy. It was indicated that MACP was a potential surface modification strategy for vascular stents candidate material.

Plateiet-Activating Factor (1-0-Alkyl-2-Acetyl-sn-Glyceryl-3-Phosphorycholine) Stimulates Prostacyclin Synihesis By Cultured Human Endothelial Cells

1981 ◽  
Author(s):  
S T Test ◽  
N U Bang
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Platelet Rich Plasma ◽  
Umbilical Vein ◽  
Platelet Activating Factor ◽  
Biological Properties ◽  
Feedback Mechanism ◽  
Negative Feedback Mechanism ◽  
Cell Layers ◽  
Umbilical Vein Endothelial Cells

l-0-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine (Ac-GEPC) has recently been established to possess biological properties identical to those of platelet-activating factor (PAF) released from stimulated mast cells and leucocytes (Demopoulos et al, J. Biol. Chem. 254:9355, 1979). Shaw et al (J. Imuunol. 121:1939, 1978) reported that PAF is capable of stimulating platelet thranbaxane A2 synthesis. We examine here whether synthetic Ac-GEPC stimulates PGI2 synthesis in human endothelial cell cultures. Human umbilical vein endothelial cells during the third passage were grown to confluency in 35 x 10 ran dishes and challenged with Na arachidonate(NaA), 8 x 10-5M or Ac-GEPC, 5 x 10-% (a concentration which produced optimal platelet aggregation in human platelet-rich plasma). PGI2 production was monitored through radioimmunoassays of its metabolite, 6-keto-PGF1α. After washing endothelial cell layers twice, the agonists were added in tris-Tyrodes buffer, pH 7.2 containing 2.5 g/L bovine serum albumin. Representative timed experiments produced the following results expressed in pM 6-keto-PGF1α/105 cells for NaA and Ac-GEPC respectively after the noted times of incubation: 10s: 274/467, 20s: 630/1136, 30s: 1111/1005, 60s: 1757/1217, 90s: 1842/1272, 120s: 1115/698, 180s: 1188/453. These data indicate that Ac-GEPC stimulates the production of PGI2 at a rate roughly comparable to that achieved by stimulation with NaA, reaching a maximum at 60 and 90s in these experiments. The decrease, compared to peak levels, in iranunoreactive 6-keto-PGF1α at 120 and 180s of 23% and 36% for NaA stimulated cells and 45% and 65% for Ac-GEPC stimulated cells suggests that enzymes are activated during the experiment which cause conversion of PGI2 into metabolites other than 6-keto-PGF1α (e.g. 6-keto-PGE1). These results suggest another theoretically important negative feedback mechanism for a potent platelet proaggregatorv substance.

scholarly journals Vascular and Blood Compatibility of Engineered Cationic Cellulose Nanocrystals in Cell-Based Assays

Nanomaterials ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2072
Author(s):  
Alexandre Bernier ◽  
Tanner Tobias ◽  
Hoang Nguyen ◽  
Shreshth Kumar ◽  
Beza Tuga ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Cell Viability ◽  
Cellulose Nanocrystals ◽  
Blood Compatibility ◽  
Biological Activities ◽  
Umbilical Vein ◽  
Circulatory System ◽  
Dependent Manner ◽  
Umbilical Vein Endothelial Cells ◽  
The Impact

An emerging interest regarding nanoparticles (NPs) concerns their potential immunomodulatory and pro-inflammatory activities, as well as their impact in the circulatory system. These biological activities of NPs can be related to the intensity and type of the responses, which can raise concerns about adverse side effects and limit the biomedical applicability of these nanomaterials. Therefore, the purpose of this study was to investigate the impact of a library of cationic cellulose nanocrystals (CNCs) in the human blood and endothelial cells using cell-based assays. First, we evaluated whether the cationic CNCs would cause hemolysis and aggregation or alteration on the morphology of red blood cells (RBC). We observed that although these nanomaterials did not alter RBC morphology or cause aggregation, at 24 h exposure, a mild hemolysis was detected mainly with unmodified CNCs. Then, we analyzed the effect of various concentrations of CNCs on the cell viability of human umbilical vein endothelial cells (HUVECs) in a time-dependent manner. None of the cationic CNCs caused a dose-response decrease in the cell viability of HUVEC at 24 h or 48 h of exposure. The findings of this study, together with the immunomodulatory properties of these cationic CNCs previously published, support the development of engineered cationic CNCs for biomedical applications, in particular as vaccine nanoadjuvants.

scholarly journals Use of nPSi-βCD Composite Microparticles for the Controlled Release of Caffeic Acid and Pinocembrin, Two Main Polyphenolic Compounds Found in a Chilean Propolis

Pharmaceutics ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 289 ◽  
Author(s):  
Dina Guzmán-Oyarzo ◽  
Tanya Plaza ◽  
Gonzalo Recio-Sánchez ◽  
Dulcineia S. P. Abdalla ◽  
Luis A. Salazar ◽  
...  
Keyword(s):  
Controlled Release ◽  
Caffeic Acid ◽  
Oral Delivery ◽  
Umbilical Vein ◽  
Physicochemical Characterization ◽  
Biological Properties ◽  
Suitable Material ◽  
Therapeutic Properties ◽  
Main Components ◽  
Umbilical Vein Endothelial Cells

Propolis is widely recognized for its various therapeutic properties. These are attributed to its rich composition in polyphenols, which exhibit multiple biological properties (e.g., antioxidant, anti-inflammatory, anti-angiogenic). Despite its multiple benefits, oral administration of polyphenols results in low bioavailability at the action site. An alternative to face this problem is the use of biomaterials at nano-micro scale due to its high versatility as carriers and delivery systems of various drugs and biomolecules. The aim of this work is to determine if nPSi-βCD microparticles are a suitable material for the load and controlled release of caffeic acid (CA) and pinocembrin (Pin), two of the main components of a Chilean propolis with anti-atherogenic and anti-angiogenic activity. Polyphenols and nPSi-βCD microparticles cytocompatibility studies were carried out with human umbilical vein endothelial cells (HUVECs). Results from physicochemical characterization demonstrated nPSi-βCD microparticles successfully retained and controlled release CA and Pin. Furthermore, nPSi-βCD microparticles presented cytocompatibility with HUVECs culture at concentrations of 0.25 mg/mL. These results suggest that nPSi-βCD microparticles could safely be used as an alternate oral delivery system to improve controlled release and bioavailability of CA or Pin—and eventually other polyphenols—thus enhancing its therapeutic effect for the treatment of different diseases.

scholarly journals Origin and Characterization of Extracellular Vesicles Present in the Spider Venom of Ornithoctonus hainana

Toxins ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 579
Author(s):  
Chengfeng Xun ◽  
Lu Wang ◽  
Hailin Yang ◽  
Zixuan Xiao ◽  
Min Deng ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Umbilical Vein ◽  
Gradient Centrifugation ◽  
Biological Properties ◽  
Bioactive Molecules ◽  
Spider Venom ◽  
Cellular Origin ◽  
Venom Glands ◽  
Umbilical Vein Endothelial Cells ◽  
Related Proteins

Extracellular vesicles (EVs), including exosomes and microvesicles, are membranous vesicles released from nearly all cellular types. They contain various bioactive molecules, and their molecular composition varies depending on their cellular origin. As research into venomous animals has progressed, EVs have been discovered in the venom of snakes and parasitic wasps. Although vesicle secretion in spider venom glands has been observed, these secretory vesicles’ origin and biological properties are unknown. In this study, the origin of the EVs from Ornithoctonus hainana venom was observed using transmission electron microscopy (TEM). The Ornithoctonus hainana venom extracellular vesicles (HN-EVs) were isolated and purified by density gradient centrifugation. HN-EVs possess classic membranous vesicles with a size distribution ranging from 50 to 150 nm and express the arthropod EV marker Tsp29Fb. The LC-MS/MS analysis identified a total of 150 proteins, which were divided into three groups according to their potential function: conservative vesicle transport-related proteins, virulence-related proteins, and other proteins of unknown function. Functionally, HN-EVs have hyaluronidase activity and inhibit the proliferation of human umbilical vein endothelial cells (HUVECs) by affecting the cytoskeleton and cell cycle. Overall, this study investigates the biological characteristics of HN-EVs for the first time and sheds new light on the envenomation process of spider venom.

Preparation and Biological Properties of Nano-Silk Fibroin/Polyurethane/Silver Nanoparticles Composite Membranes

2016 ◽  
Vol 848 ◽  
pp. 557-566 ◽  
Author(s):  
Jiang Feng Li ◽  
Zhong Min Chen ◽  
Qin Luo ◽  
Guang Hui Zhang ◽  
Fu Ping Wang
Keyword(s):  
Silver Nanoparticles ◽  
Silver Nitrate ◽  
Silk Fibroin ◽  
Influence Factor ◽  
Blood Compatibility ◽  
Antibacterial Properties ◽  
Composite Membranes ◽  
Biological Properties ◽  
Mass Ratio ◽  
Uv Spectrum

A series of the blending membranes comprised of the different mass ratio of silk fibroin peptide (SFP) power, silver nanoparticles (NanoAg) and polyurethane (PU) were prepared with mixing the SFP power, silver nitrate and PU, and the silver nitrate was reduced to NanoAg by SFP in this mixing process. UV spectrum was used to detect the nanoAg. The experimental results confirmed the formation of NanoAg. The dissolution of the obtained membranes in simulated body fluid (SBF) were determined, and showed that the membranes had good stability. Their mechanical properties were also determined. The results revealed that the mass ratio of SFP to PU was an important influence factor, and the mass ratio 30/70 (SFP/PU) of the membrane was the optimum. The Antibacterial properties against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa, were examined, the results of which indicated that the membranes containing NanoAg had a good bactericidal effect. The SFP/PU/NanoAg membranes also showed good cell and blood compatibility, and then they have application prospect as an antibacterial materials using for medical catheters.

Ultrastructural observations on the in vitro cytoadherence of Plasmodium falciparum infected red blood cells

1990 ◽  
Vol 48 (3) ◽  
pp. 914-915
Author(s):  
D.J.P. Ferguson ◽  
A.R. Berendt ◽  
J. Tansey ◽  
K. Marsh ◽  
C.I. Newbold
Keyword(s):  
Plasmodium Falciparum ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Umbilical Vein ◽  
Cell Types ◽  
Amelanotic Melanoma ◽  
Cytoplasmic Process ◽  
Umbilical Vein Endothelial Cells

In human malaria, the most serious clinical manifestation is cerebral malaria (CM) due to infection with Plasmodium falciparum. The pathology of CM is thought to relate to the fact that red blood cells containing mature forms of the parasite (PRBC) cytoadhere or sequester to post capillary venules of various tissues including the brain. This in vivo phenomenon has been studied in vitro by examining the cytoadherence of PRBCs to various cell types and purified proteins. To date, three Ijiost receptor molecules have been identified; CD36, ICAM-1 and thrombospondin. The specific changes in the PRBC membrane which mediate cytoadherence are less well understood, but they include the sub-membranous deposition of electron-dense material resulting in surface deformations called knobs. Knobs were thought to be essential for cytoadherence, lput recent work has shown that certain knob-negative (K-) lines can cytoadhere. In the present study, we have used electron microscopy to re-examine the interactions between K+ PRBCs and both C32 amelanotic melanoma cells and human umbilical vein endothelial cells (HUVEC).We confirm previous data demonstrating that C32 cells possess numerous microvilli which adhere to the PRBC, mainly via the knobs (Fig. 1). In contrast, the HUVEC were relatively smooth and the PRBCs appeared partially flattened onto the cell surface (Fig. 2). Furthermore, many of the PRBCs exhibited an invagination of the limiting membrane in the attachment zone, often containing a cytoplasmic process from the endothelial cell (Fig. 2).

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