Inductive Effect of Human Recombinant IL-1 on Differentiation of a Macrophage-Like Tumor Cell Line

1988 ◽  
Vol 2 (2) ◽  
pp. 372-375 ◽  
Author(s):  
S. Hanazawa ◽  
S. Amano ◽  
C. Hanaizumi ◽  
K. Hirose ◽  
Y. Ohmori ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Line ◽  
Inductive Effect ◽  
Interleukin 1 ◽  
Tumor Cell Line ◽  
Receptor Expression ◽  
Local Factor ◽  
Dose Dependent Fashion ◽  
Dose Dependent ◽  
Stimulation Of

We used the macrophage-like tumor cell line P388D1 to test whether interleukin-1 (IL-1) stimulate differentiation of osteoclast-like progenitors. Recombinant human interleukin-1 (rhIL-1) alpha inhit ited cell growth in a dose-dependent fashion. Incubation of the cells with rhIL-1 alpha resulted in adherence stimulation of non-specific esterase activity, and increased Fc receptor expression. These results suggest th possibility that IL-1 may be involved in the differentiation of osteoclast progenitors and thus may be an irr portant local factor in the mechanism of bone resorption.

Inductive effect of recombinant human interleukin-1 ? and ? on differentiation of macrophage-like tumor cell line P388D1

1988 ◽  
Vol 136 (3) ◽  
pp. 543-546 ◽  
Author(s):  
Shigemasa Hanazawa ◽  
Chika Hanaizumi ◽  
Shigeru Amano ◽  
Kimiharu Hirose ◽  
Yoshihiro Ohmori ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Line ◽  
Inductive Effect ◽  
Interleukin 1 ◽  
Tumor Cell Line

Modulation of Interleukin-1 Receptors Followed by Endotoxin Lipopolysaccharide Treatment in the Mouse AtT-20 Pituitary Tumor Cell Line

2002 ◽  
Vol 10 (5) ◽  
pp. 310-316 ◽  
Author(s):  
Chizuru Kumagai ◽  
Toshihiro Takao ◽  
Reiko Matsumoto ◽  
Koichi Asaba ◽  
Kozo Hashimoto
Keyword(s):  
Tumor Cell ◽  
Cell Line ◽  
Pituitary Tumor ◽  
Interleukin 1 ◽  
Tumor Cell Line ◽  
Pituitary Tumor Cell

scholarly journals Aminoglutethimide suppresses adrenocorticotropin receptor expression in the NCI-h295 adrenocortical tumor cell line

1998 ◽  
Vol 159 (1) ◽  
pp. 35-42 ◽  
Author(s):  
M Fassnacht ◽  
F Beuschlein ◽  
S Vay ◽  
P Mora ◽  
B Allolio ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Cell Line ◽  
Cdna Probe ◽  
Tumor Cell Line ◽  
Receptor Expression ◽  
Intracellular Camp ◽  
Camp Accumulation ◽  
Transcript Accumulation ◽  
Down Regulation ◽  
Stimulation Of

The adrenostatic compound aminoglutethimide (AG), a potent inhibitor of the P450 side chain cleavage enzyme, is used in the treatment of ACTH-dependent or adrenal Cushing's syndrome. Recently, AG has been shown to inhibit ACTH receptor (ACTH-R) mRNA expression in ovine adrenocortical cells in a time-dependent fashion. To investigate whether ACTH-R down-regulation will also be induced in tumor cells, we studied the effect of AG on ACTH-R expression in the human NCI-h295 adrenocortical carcinoma cell line, which expresses functional ACTH receptors and produces steroids of the glucocorticoid, mineralocorticoid and androgen pathway. The cells were incubated in triplicate with increasing doses of AG (3, 30, 300 microM) which suppressed steroid secretion dose-dependently. After 48 h, cells were harvested, and total RNA was extracted, electrophoresed, blotted and hybridized with a human ACTH-R cDNA probe. In parallel experiments, after preincubation with AG the cells were stimulated with ACTH (10 nM) for 10 min and the intracellular cAMP accumulation was determined by RIA. AG significantly suppressed the baseline ACTH-R mRNA expression in a dose-dependent fashion (300 microM AG, 5+/-1%; 30 microM AG, 64+/-1%; 3 microM AG, 108+/-19% compared with control cells, 100+/-11%). The reduced ACTH-R mRNA expression was paralleled by low ACTH-induced cAMP accumulation indicating reduced expression of the ACTH-R protein. The adrenostatic compound metyrapone, an inhibitor of 11beta-hydroxylase activity, also suppressed ACTH-R mRNA expression in a similar fashion. Stimulation of the protein kinase A pathway by simultaneous incubation of ACTH (10 nM) or forskolin (10 microM) together with AG was not able to overcome the steroid biosynthesis blockade, but reversed the inhibitory effects of AG on the ACTH-R mRNA expression. Also, cortisol (12 microM) reversed the AG-induced ACTH-R mRNA expression. We conclude that AG induces profound ACTH-R down-regulation in the NCI-h295 cell line either by affecting the gene expression or by decreasing transcript accumulation via an effect on RNA stability. This novel action of AG can be reversed by stimulation of the cAMP pathway and of the glucocorticoid-mediated signal transduction cascade. As the down-regulation occurs in vitro at concentrations which are reached during treatment with AG in humans it may contribute to its therapeutic activity in adrenal disease.

Stimulation of Ki-ras Ribozyme Activity by RNA Binding Protein, NCp7, in Vitro and in Pancreatic Tumor Cell Line, Capan 1

1994 ◽  
Vol 733 (1 Molecular and) ◽  
pp. 113-121 ◽  
Author(s):  
KARIN MOELLING ◽  
GERD MUELLER ◽  
JENS DANNULL ◽  
CHRISTOPH REUSS ◽  
PETER BEIMLING ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Line ◽  
Pancreatic Tumor ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Tumor Cell Line ◽  
Pancreatic Tumor Cell ◽  
Stimulation Of

Cyclic AMP-dependent modulation of interleukin-1 receptors in the mouse AtT-20 pituitary tumor cell line

1994 ◽  
Vol 656 (1) ◽  
pp. 177-181 ◽  
Author(s):  
Toshihiro Takao ◽  
Klaus D. Dieterich ◽  
Daniel E. Tracey ◽  
Errol B. De Souza
Keyword(s):  
Cyclic Amp ◽  
Tumor Cell ◽  
Cell Line ◽  
Pituitary Tumor ◽  
Interleukin 1 ◽  
Tumor Cell Line ◽  
Pituitary Tumor Cell

Activation of cyclic AMP-dependent protein kinase activity during LPS stimulation of macrophage tumor cell line, J774.1

1981 ◽  
Vol 3 (1) ◽  
pp. 57-66 ◽  
Author(s):  
Hitoshi Kikutani ◽  
Tadamitsu Kishimoto ◽  
Nobuo Sakaguchi ◽  
Yoshio Nishizawa ◽  
Peter Ralph ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Cyclic Amp ◽  
Tumor Cell ◽  
Cell Line ◽  
Kinase Activity ◽  
Tumor Cell Line ◽  
Protein Kinase Activity ◽  
Dependent Protein Kinase ◽  
Dependent Protein ◽  
Stimulation Of
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