Vitamin C ameliorates the adverse effects of dexamethasone on sperm motility, testosterone level, and spermatogenesis indexes in mice

2018 ◽  
Vol 38 (4) ◽  
pp. 409-418 ◽  
Author(s):  
F Sadeghzadeh ◽  
MS Mehranjani ◽  
M Mahmoodi
Keyword(s):  
Adverse Effects ◽  
Vitamin C ◽  
Sperm Motility ◽  
Testosterone Level ◽  
Leydig Cells ◽  
Serum Testosterone ◽  
Serum Testosterone Level ◽  
Control Group ◽  
The Mean ◽  
Vit C

Background: Dexamethasone (DEX) is a common medicine that is capable of causing malformation in the male reproductive system. The aim of this study was to investigate the effect of vitamin C (Vit-C) on spermatogenesis indexes and daily sperm production (DSP) in adult mice treated with DEX. Methods: Male Naval Medical Research Institute (NMRI) mice were divided into four groups: Control, DEX (7 mg/kg/day), Vit-C (100 mg/kg/day), and DEX +Vit-C and treated for 7 days with intraperitoneal injection. Results: A significant increase in the mean levels of serum and tissue malondialdehyde (MDA) and apoptosis of Leydig cells was found in the DEX group compared to the control group. Sperm motility, DSP, tubular differentiation index, meiotic index, spermatogenesis index, the mean number of spermatocytes, round and long spermatids, and Leydig cells, and also serum testosterone level decreased in the DEX group compared to the control group. The results of this study indicate that Vit-C can significantly prevent the adverse effects of DEX on the mean number of spermatocyte, spermatid, and Leydig cells, tubular differentiation, meiotic and spermatogenesis index, DSP, sperm motility, and the mean levels of serum MDA. Conclusion: In conclusion, our results showed that coadministration of Vit-C and DEX prevents the adverse effects of DEX on the spermatogenesis indexes and DSP.

scholarly journals Effects of curcumin on sperm parameters abnormalities induced by morphine in rat

2017 ◽  
Vol 6 (2) ◽  
pp. 1-10
Author(s):  
S.H. Roshankhah ◽  
M.R. Salahshoor ◽  
S. Aryanfar ◽  
F. Jalili ◽  
M. Sohrabil ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Testosterone Level ◽  
Sperm Count ◽  
Serum Testosterone ◽  
Treated Group ◽  
Sperm Parameters ◽  
Male Rats ◽  
Serum Testosterone Level ◽  
Seminiferous Tubules ◽  
Control Group

Opioids are the most potent and effective analgesics available and have become accepted as appropriate treatment for acute, cancer and non-cancer. Morphine, which is commonly used for the treatment of severe pain, gastrointestinal tract and kidneys. Curcumin petals consist of, glycosides, flavonoids, and anthocyanin. The study aims at evaluating curcumin effect and morphine on sperm parameters, testis tissue and serum testosterone level in rat. In this experimental study, 48 male rats with 28 weeks of age and limited weight of 270 to 300g were selected. They were divided into eight groups of 6, untreated control group; morphine – treated group (20 mg/kg/day); curcumin -treated groups (10, 30, 60 mg/kg/day); and morphine and curcumin treated group intraperitoneal administration for successive 28 days. After 24hours animals were killed. Sperm motility was measured using WHO protocols. The sperm parameter such as motility, sperm count, morphology, seminiferous tubules diameter, weight testis, and serum testosterone level were analyzed (oneway ANOVA). Curcumin (10, 30 and 60 mg/kg) significantly increased mean percentage of sperm motility, count, testis weight, and serum testosterone level compared to control group (p<0.05). Testosterone level decreased significantly in rats treated with morphine. Co-administration of curcumin to morphine-treated rats improved the histopathological alterations induced by morphine in testis and increased the sperm count. Curcumin has a very strong antioxidant effects at applied doses and it can probably be used as an antioxidant and food supplement in reproductive disorders.Journal of Medical and Biomedical Sciences (2017) 6(2), 1-10

scholarly journals Comparative Study on the effect of Varicocelectomy on Infertile Patients with Normal and Low Serum Testosterone Level

2021 ◽  
pp. 23-29
Author(s):  
Mohammed Saad Mohammed Sherif ◽  
Ayman Mohammed Hagras ◽  
Mohammed Hassan Radwan ◽  
Mohammed Abo El-enen Khalwash
Keyword(s):  
Comparative Study ◽  
Testosterone Level ◽  
Serum Testosterone ◽  
University Hospital ◽  
Semen Parameters ◽  
Serum Testosterone Level ◽  
Male Population ◽  
Group A ◽  
The Mean ◽  
Group B

Introduction: Varicocele is a dilation of the pampiniform venous plexus and the internal Spermatic vein. It is detected approximately in 15% to 20 % of the general male Population; with the prevalence increasing to 40% in infertile men. Methodology: This prospective, comparative study was conducted on 134 male patients with primary infertility who attended the outpatient clinic of Urology Department, Tanta University Hospital during the period between April 2017 to April 2019. The patients were divided into two groups: Group A (56 patients) with low testosterone level. Group B (78 patients) with normal testosterone level. Results: In group A, the mean ± SD of testosterone level improved significantly to 2.93 ± 0.77 and 3.36 ± 0.86 after 3 and 6 months respectively (p1-value=0.001* and p2-value=0.001* respectively). In group B, the mean ± SD of testosterone level improved significantly to 6.22 ± 1.51 and 6.82 ± 2.25 after 3 and 6 months respectively (p1-value=0.026* and p2-value=0.001* respectively). Conclusion: varicocelectomy could improve semen parameters, serum testosterone level and pregnancy rate.

Evaluate the Effect of Amlodipine Besylate on Testosterone and Fertility of Male albino Rats

2020 ◽  
Author(s):  
Shahid Ali
Keyword(s):  
Testosterone Level ◽  
Channel Blocker ◽  
Serum Testosterone ◽  
Serum Testosterone Level ◽  
Control Group ◽  
Albino Rats ◽  
Distilled Water ◽  
Ca Channel ◽  
Amlodipine Besylate ◽  
Group B

Background: Amlodipine is a Ca++ channel blocker, routinely used for treatment of hypertension. The mechanism underlying its anti-hypertensive effect is dilatation of blood vessels. Testosterone, the hormone produced by testis, is responsible for development of male sexual secondary characteristics. The aim of study was to find out the effect of Ca++ channel blocker, Amlodipine besylate on serum testosterone level in male albino Rats. Methods: Duration of study was 30 days and conducted in department of pharmacology, 24 male Albino rats were grouped into 4 groups (each having 6). Group A was control group, Group B, C and D were treated with Amlodipine Besylate drug at doses 0.07, 0.14 and 0.28mg/kg body weight respectively (Suspension of 5 mg tablet of Amlodipine dissolved in 10 ml distilled water prepared and further diluted to 1:10ml with distilled water). Independent t test and ANOVA with Tukey’s test were used for comparison of quantitative variables with in groups. Results: Serum testosterone level reduced in groups treated with Amlodipine. Serum testosterone level of control group is 11.22 while group B, C and D were 4.7, 2.6 and 0.7 respectively. Serum FSH level of control group is 0.10±0.01 while group B, C and D were 0.24±0.35, 0.34±0.40 and 0.08±0.03 respectively. It showed that increasing the dose of Amlodipine markedly reduce testosterone level and increases FSH level in male albino Rats. Conclusion: Amlodipine severely reduce level of serum testosterone (p=0.001) and increases FSH level (p=0.305) which can lead to infertility in male albino Rats.

scholarly journals Estimate the level of testosterone hormone using I-chroma technique in males treated with Royal jelly and honeybee

2021 ◽  
Vol 4 (1) ◽  
pp. 16-18
Author(s):  
Baqer Hassan ◽  
Zainab Fadhil Abbas
Keyword(s):  
Testosterone Level ◽  
Royal Jelly ◽  
Serum Testosterone ◽  
Treated Group ◽  
Serum Testosterone Level ◽  
Control Group ◽  
Short Term Treatment ◽  
Testosterone Levels ◽  
Natural Treatment ◽  
Result Show

The experiment based on 10 matured men samples collected randomly from Baghdad Providence. The 10 samples divided into two groups, serum testosterone level of all 10 patient had been done using Ichroma technique. the treated group administered 2.5ml of honeybee with 350mg of royal jelly daily for (15day) while the second group stay as control group. After 15 days blood samples had been collected and serum separated to made another testosterone test for both groups using same i-chroma technique. The main Aim of this study is estimating the effect of royal jelly and honeybee as food on the testosterone hormone level in men. The result show that there was significant increase in the testosterone levels in treated group in comparison with control group. after experiment the result show significant increase in testosterone levels (in samples number 1,2,3,4 and 5) as the following (23.2 n.mo/L, 16.6 n.mo/L, 17.7 n.mo/L, 10.1 n.mo/L and 29.7 n.mo/L respectively. The study concluded that, short term treatment of royal jelly in combined with honey bee increasing the level of blood testosterone hormone in men in different ages significantly. The study either concluded that we can used honeybee and royal jelly as natural treatment of low testosterone level in men.

scholarly journals Effect of oral injection of tramadol, sildenafil and combined of tramadol and sildenafil on serum testosterone level for Wistar breed male rats

2013 ◽  
Vol 11 (9) ◽  
pp. 2959-2966
Author(s):  
Rabie Said Farag ◽  
Mohamed Abdel El Nawawy ◽  
Wael Mohamed Fathy ◽  
Hamada Atiaa
Keyword(s):  
Erectile Dysfunction ◽  
Serum Testosterone ◽  
Water Soluble ◽  
Pharmacological Effect ◽  
Male Rats ◽  
Absolute Bioavailability ◽  
Serum Testosterone Level ◽  
Control Group ◽  
Testosterone Levels ◽  
The Mean

Introduction:Testosterone is the main sexual hormone in human males, and has a pharmacological effect on the physiology of sexual function. It is known that suppression of testosterone in eugonadal adult males leads to reduced sexual desire and activity, and may result in erectile dysfunction. Tramadol is rapidly and almost completely absorbed after oral administration but its absolute bioavailability is only 65–70% due to first-pass metabolism. Sildenafil citrate is a water soluble citrate salt that was firstly synthesized by Pfizer in United Kingdom to treat hypertension and angina pectoris. Interestingly, this drug exhibited a different pharmacological effect, a marked penile erection, and became the first-line treatment option to erectile dysfunction. Aim of the work :the main work goal of this search was to measure and compare between mean serum testosterone concentrations after oral injection of tramadol, sildenafil , and combination of tramadol and sildenafil beside healthy subjects.Investigated Parameter: quantitative analysis of Free Testosterone ELISA Kit in serum by The Calbiotech, Inc. 10461 Austin Dr, Spring Valley, CA U.S.A., depending on McCann, D., et al 1985.Results In the present study, the results of GT group revealed that administration of tramadol 50 mg /Kg/day for 10, 20 days and 30 days indicated that the measured testosterone levels were lower than the (GC) control group, and the decrease in testosterone mean concentrations in GT3, than GT2, than GT1. In our research GS group administration of sildenafil 50 mg /Kg/day for 10, 20 and 30 days groups indicated that increase testosterone levels than that in control GC group, and there was an increase in testosterone mean concentrations in GS3, than GS2, than GS1. The present study reveled in GT&S administration of tramadol 50 mg /Kg/day combined with sildenafil 50 mg /Kg/day that the mean serum testosterone concentrations in (GT&S1), (GT&S2) and (GT&S3), were decrease than in the mean serum testosterone concentrations (GC), but this decrease in the mean serum testosterone concentrations in (GT&S1), (GT&S2) and (GT&S3), were quite higher than mean serum testosterone concentrations in GT1, GT2, and GT3, also and there were a decrease in mean testosterone mean concentrations in (GT&S3) than that in (GT&S2) and (GT&S1)conclusion: tramadol administration decrease testosterone levels, while sildenafil increase testosterone levels, while the combination between tramadol & sildenafil decrease testosterone levels.

AMELIORATIVE EFFECTS OF MGS04 ON DEXAMETHASONE- INDUCED HISTO-CHEMICAL ALTERATIONS IN THE TESTES OF ALBINO RATS

1969 ◽  
Vol 6 (1) ◽  
pp. 753-757
Author(s):  
INAYATULLAH ◽  
MOHAMMAD KHAN ◽  
MARIYA HIDAYAT ◽  
LUTFUR-RAHMAN ◽  
ASMA SIDDIQUI ◽  
...  
Keyword(s):  
Testosterone Level ◽  
Serum Testosterone ◽  
Concomitant Administration ◽  
Testicular Tissue ◽  
Serum Testosterone Level ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Albino Rats ◽  
Male Adult ◽  
Group B

BACKGROUND: In experimental animal, dexamethasone-induced impaired spermatogenesis causesdisruption of the normal architecture of seminiferous tubules and alteration in male sexual hormonetestosterone. Concomitant administration of MgS04 preserved the cytoarchitecture of testes as well ashormonal regulation in albino rats.OBJECTIVE: This study was designed to observe the ameliorative effects of MgS04 on the histologyof testes and there correlation with serum testosterone level during dexamethasone administration inalbino rats.METHODOLOGY: This experimental study was conducted in the department of Anatomy, BasicA LMedical Sciences Institute (BMSI), Jinnah Postgraduate Medical Centre (JPMC), Karachi from 5 Aprilto 25th April 2012. Thirty healthy male adult albino rats were included in the study and divided equallyinto 3 groups. Each group comprising 10 animals. Group-A served as Control. Group-B receivedDexamethasone (intraperitonealy) at the dose of 4mg/kg body weight/24 hours. Group-C receivedDexamethasone at the same dose as in group-B and additionally given MgS04 (intramuscularly) at thedose of 20mg/kg/24hours.RESULTS: MgS04 significantly preserved the cytoarchitecture of testes, as well as minimizedalteration in serum testosterone level in group-C animals.CONCLUSION: MgSO4 has restored both the histological and biochemical damaging effects inducedby dexamethasone in Rats testes.KEYWORDS: Dexamethasone, MgS04, Testicular tissue, Testosterone.

scholarly journals Testosterone role during seasons changes in the dogs testes

2017 ◽  
Vol 41 (1) ◽  
pp. 125-130
Author(s):  
Nazih Wayes Zaid
Keyword(s):  
Sertoli Cells ◽  
Testosterone Level ◽  
Leydig Cells ◽  
Hormone Receptors ◽  
Winter Season ◽  
Serum Testosterone ◽  
Reproductive Activity ◽  
Summer Season ◽  
Serum Testosterone Level ◽  
Four Seasons

     This study was conducted to evaluate the effect of seasons on serum testosterone level, testis histology examination and testosterone receptors by using cytoimmunochemistry techniques. Samples of blood and both testes were collected from 24 local dogs aged between 2-3 years and weighted 18-22 kg. This study carried out at 2015, divided into four seasons. No differences in testosterone level were observed between seasons. However, histological examinations were characterized by significant (P<0.01) changes during seasons in tubular diameters, germinal thickness and tubular area. Cytoimmunochemistry investigation showed differences between seasons also, there was a distributions of testosterone hormone receptors in the Leydig cells, Sertoli cells and in germinal epithelium during spring and autumn, at the same time the winter season showed distribution of testosterone hormone receptors in Leydig and Sertoli cells only. The summer season characterize by diffuse of receptors in Leydig cells only. In conclusion there was a significant effect of season on testis of dog in Iraq, the summer season was the lowest in reproductive activity while the spring and autumn season was the highest of reproductive activity of dog in Iraq.

scholarly journals Effect of Quinolones on Serum Testosterone Level in Male Albino Rats

2017 ◽  
Vol 23 (1) ◽  
Author(s):  
Rubina Iqbal ◽  
Saud Iqbal ◽  
Shahzad Anjum
Keyword(s):  
Testosterone Level ◽  
Morphological Changes ◽  
Serum Testosterone ◽  
Serum Testosterone Level ◽  
Term Therapy ◽  
Control Group ◽  
Albino Rats ◽  
Distilled Water ◽  
Blood Samples ◽  
Testosterone Levels

<p><strong>Objective:</strong><strong>  </strong>To determine the toxic effects of quinolones on serum testosterone level in male albino rats.</p><p><strong>Methods:</strong><strong>  </strong>Eighty male albino rats were randomly divided into A, B, C and D groups each group having 20 albino rats. These groups were further subdivided into A1, A2, B1, B2, C1, C2, D1 and D2 having 10 albino rats each. Ciprofloxcin, ofloxacin and enoxacin dissolved in distilled water were given at 135mg/kg/ day, 72mg/kg/day and 12.5mg/kg/day to groups A, B and C respectively for 12 weeks. Only distilled water was given to group D which was control group for the same time period. Blood samples were drawn for testosterone hormone level estimation at 0, 14<sup>th</sup>, 28<sup>th</sup> and 42<sup>nd</sup> day in subgroups Al, B1, Cl and D1 and then the animals in said groups were sacrificed on 42<sup>nd</sup> day to identify testicular morphological changes. Rats in subgroup A2, B2, C2 and D2 were kept alive till 84<sup>th</sup> day after stopping drugs at 42 days to find out if there is any change in levels of testosterone after discontinuation of the treatment. Blood samples for testosterone hormone estimation were taken at 0, 14<sup>th</sup>, 28<sup>th</sup>, 42<sup>nd, </sup>56<sup>th</sup>, 70<sup>th</sup> and 84<sup>th</sup> day from subgroups A2, B2, C2 and D2. Testosterone hormone estimation assay was performed on the collected samples.</p><p><strong>Results:</strong><strong>  </strong>Testosterone assay showed significant decrease in serum testosterone levels in all experimental groups, when compared with control group. These levels did not return back to normal even after withdrawal of drugs. This study suggests a gonadotoxic potential of quinolones.</p><p><strong>Conclusion:  </strong>Quinolones reduce serum testosterone levels and should be used carefully for long term therapy.<strong></strong></p>

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