Evaluate the Effect of Amlodipine Besylate on Testosterone and Fertility of Male albino Rats

2020 ◽  
Author(s):  
Shahid Ali
Keyword(s):  
Testosterone Level ◽  
Channel Blocker ◽  
Serum Testosterone ◽  
Serum Testosterone Level ◽  
Control Group ◽  
Albino Rats ◽  
Distilled Water ◽  
Ca Channel ◽  
Amlodipine Besylate ◽  
Group B

Background: Amlodipine is a Ca++ channel blocker, routinely used for treatment of hypertension. The mechanism underlying its anti-hypertensive effect is dilatation of blood vessels. Testosterone, the hormone produced by testis, is responsible for development of male sexual secondary characteristics. The aim of study was to find out the effect of Ca++ channel blocker, Amlodipine besylate on serum testosterone level in male albino Rats. Methods: Duration of study was 30 days and conducted in department of pharmacology, 24 male Albino rats were grouped into 4 groups (each having 6). Group A was control group, Group B, C and D were treated with Amlodipine Besylate drug at doses 0.07, 0.14 and 0.28mg/kg body weight respectively (Suspension of 5 mg tablet of Amlodipine dissolved in 10 ml distilled water prepared and further diluted to 1:10ml with distilled water). Independent t test and ANOVA with Tukey’s test were used for comparison of quantitative variables with in groups. Results: Serum testosterone level reduced in groups treated with Amlodipine. Serum testosterone level of control group is 11.22 while group B, C and D were 4.7, 2.6 and 0.7 respectively. Serum FSH level of control group is 0.10±0.01 while group B, C and D were 0.24±0.35, 0.34±0.40 and 0.08±0.03 respectively. It showed that increasing the dose of Amlodipine markedly reduce testosterone level and increases FSH level in male albino Rats. Conclusion: Amlodipine severely reduce level of serum testosterone (p=0.001) and increases FSH level (p=0.305) which can lead to infertility in male albino Rats.

AMELIORATIVE EFFECTS OF MGS04 ON DEXAMETHASONE- INDUCED HISTO-CHEMICAL ALTERATIONS IN THE TESTES OF ALBINO RATS

1969 ◽  
Vol 6 (1) ◽  
pp. 753-757
Author(s):  
INAYATULLAH ◽  
MOHAMMAD KHAN ◽  
MARIYA HIDAYAT ◽  
LUTFUR-RAHMAN ◽  
ASMA SIDDIQUI ◽  
...  
Keyword(s):  
Testosterone Level ◽  
Serum Testosterone ◽  
Concomitant Administration ◽  
Testicular Tissue ◽  
Serum Testosterone Level ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Albino Rats ◽  
Male Adult ◽  
Group B

BACKGROUND: In experimental animal, dexamethasone-induced impaired spermatogenesis causesdisruption of the normal architecture of seminiferous tubules and alteration in male sexual hormonetestosterone. Concomitant administration of MgS04 preserved the cytoarchitecture of testes as well ashormonal regulation in albino rats.OBJECTIVE: This study was designed to observe the ameliorative effects of MgS04 on the histologyof testes and there correlation with serum testosterone level during dexamethasone administration inalbino rats.METHODOLOGY: This experimental study was conducted in the department of Anatomy, BasicA LMedical Sciences Institute (BMSI), Jinnah Postgraduate Medical Centre (JPMC), Karachi from 5 Aprilto 25th April 2012. Thirty healthy male adult albino rats were included in the study and divided equallyinto 3 groups. Each group comprising 10 animals. Group-A served as Control. Group-B receivedDexamethasone (intraperitonealy) at the dose of 4mg/kg body weight/24 hours. Group-C receivedDexamethasone at the same dose as in group-B and additionally given MgS04 (intramuscularly) at thedose of 20mg/kg/24hours.RESULTS: MgS04 significantly preserved the cytoarchitecture of testes, as well as minimizedalteration in serum testosterone level in group-C animals.CONCLUSION: MgSO4 has restored both the histological and biochemical damaging effects inducedby dexamethasone in Rats testes.KEYWORDS: Dexamethasone, MgS04, Testicular tissue, Testosterone.

scholarly journals Effect of Quinolones on Serum Testosterone Level in Male Albino Rats

2017 ◽  
Vol 23 (1) ◽  
Author(s):  
Rubina Iqbal ◽  
Saud Iqbal ◽  
Shahzad Anjum
Keyword(s):  
Testosterone Level ◽  
Morphological Changes ◽  
Serum Testosterone ◽  
Serum Testosterone Level ◽  
Term Therapy ◽  
Control Group ◽  
Albino Rats ◽  
Distilled Water ◽  
Blood Samples ◽  
Testosterone Levels

<p><strong>Objective:</strong><strong>  </strong>To determine the toxic effects of quinolones on serum testosterone level in male albino rats.</p><p><strong>Methods:</strong><strong>  </strong>Eighty male albino rats were randomly divided into A, B, C and D groups each group having 20 albino rats. These groups were further subdivided into A1, A2, B1, B2, C1, C2, D1 and D2 having 10 albino rats each. Ciprofloxcin, ofloxacin and enoxacin dissolved in distilled water were given at 135mg/kg/ day, 72mg/kg/day and 12.5mg/kg/day to groups A, B and C respectively for 12 weeks. Only distilled water was given to group D which was control group for the same time period. Blood samples were drawn for testosterone hormone level estimation at 0, 14<sup>th</sup>, 28<sup>th</sup> and 42<sup>nd</sup> day in subgroups Al, B1, Cl and D1 and then the animals in said groups were sacrificed on 42<sup>nd</sup> day to identify testicular morphological changes. Rats in subgroup A2, B2, C2 and D2 were kept alive till 84<sup>th</sup> day after stopping drugs at 42 days to find out if there is any change in levels of testosterone after discontinuation of the treatment. Blood samples for testosterone hormone estimation were taken at 0, 14<sup>th</sup>, 28<sup>th</sup>, 42<sup>nd, </sup>56<sup>th</sup>, 70<sup>th</sup> and 84<sup>th</sup> day from subgroups A2, B2, C2 and D2. Testosterone hormone estimation assay was performed on the collected samples.</p><p><strong>Results:</strong><strong>  </strong>Testosterone assay showed significant decrease in serum testosterone levels in all experimental groups, when compared with control group. These levels did not return back to normal even after withdrawal of drugs. This study suggests a gonadotoxic potential of quinolones.</p><p><strong>Conclusion:  </strong>Quinolones reduce serum testosterone levels and should be used carefully for long term therapy.<strong></strong></p>

Vitamin C ameliorates the adverse effects of dexamethasone on sperm motility, testosterone level, and spermatogenesis indexes in mice

2018 ◽  
Vol 38 (4) ◽  
pp. 409-418 ◽  
Author(s):  
F Sadeghzadeh ◽  
MS Mehranjani ◽  
M Mahmoodi
Keyword(s):  
Adverse Effects ◽  
Vitamin C ◽  
Sperm Motility ◽  
Testosterone Level ◽  
Leydig Cells ◽  
Serum Testosterone ◽  
Serum Testosterone Level ◽  
Control Group ◽  
The Mean ◽  
Vit C

Background: Dexamethasone (DEX) is a common medicine that is capable of causing malformation in the male reproductive system. The aim of this study was to investigate the effect of vitamin C (Vit-C) on spermatogenesis indexes and daily sperm production (DSP) in adult mice treated with DEX. Methods: Male Naval Medical Research Institute (NMRI) mice were divided into four groups: Control, DEX (7 mg/kg/day), Vit-C (100 mg/kg/day), and DEX +Vit-C and treated for 7 days with intraperitoneal injection. Results: A significant increase in the mean levels of serum and tissue malondialdehyde (MDA) and apoptosis of Leydig cells was found in the DEX group compared to the control group. Sperm motility, DSP, tubular differentiation index, meiotic index, spermatogenesis index, the mean number of spermatocytes, round and long spermatids, and Leydig cells, and also serum testosterone level decreased in the DEX group compared to the control group. The results of this study indicate that Vit-C can significantly prevent the adverse effects of DEX on the mean number of spermatocyte, spermatid, and Leydig cells, tubular differentiation, meiotic and spermatogenesis index, DSP, sperm motility, and the mean levels of serum MDA. Conclusion: In conclusion, our results showed that coadministration of Vit-C and DEX prevents the adverse effects of DEX on the spermatogenesis indexes and DSP.

Evaluation of blood oxidant/antioxidant changes and testicular toxicity after subacute exposure to cadmium in albino rats: Therapeutic effect of Nigella sativa seed extracts

2020 ◽  
Vol 23 ◽  
Author(s):  
Ikenna Kingsley Uchendu ◽  
Henshaw Uchechi Okoroiwu
Keyword(s):  
Oxidative Stress ◽  
Testosterone Level ◽  
Nigella Sativa ◽  
Serum Testosterone ◽  
Seed Extract ◽  
Serum Testosterone Level ◽  
Albino Rats ◽  
Testicular Toxicity ◽  
Oral Group ◽  
Testicular Injury

Aim and Objective: Cells and tissues of the body are prone to oxidative damage as a result of increased level of reactive oxygen species and nitrogen radical beyond the detoxifying ability of the endogenous antioxidant system. This study aimed to evaluate the ameliorative effect of methanolic extracts of Nigella sativa (MENS) against cadmium-induced blood oxidative stress and testicular toxicity in albino rats. Materials and Methods: Twenty five (25) male albino rats, weighing (200±20g), were randomly grouped into five groups (A-E). Group B (Negative Control) received intraperitoneal administration of cadmium chloride (CdCl2, 5mg/kg) only, group C received CdCl2 and low dose MENS (300mg/kg, oral), group D received CdCl2 and high dose MENS (600mg/kg, oral), group E (Positive control) received CdCl2 and Vitamin C (200mg/kg, oral), for 14 days. No treatment was administered to group A (Normal control). The oxidative state of the blood was assessed by measuring the blood levels or activities of MDA, CAT, GSH and SOD; while testicular injury was assessed by measuring serum testosterone level using ELISA. The testes were harvested for histopathological examination. Results: The results showed that cadmium induced a marked elevation in the level of MDA, and a decrease in SOD, CAT and GSH levels or activities (p<0.05 or p<0.01); but no significant alteration in the serum testosterone level (p>0.05); Histopathological studies on the testes showed that cadmium significantly induced testicular injury, which was however ameliorated by the seed extract of N.sativa. Conclusion: We conclude that N.sativa seed extract is potentially testiculoprotective and attenuates oxidative stress against harmful chemical toxins such as cadmium.

scholarly journals Comparative Study on the effect of Varicocelectomy on Infertile Patients with Normal and Low Serum Testosterone Level

2021 ◽  
pp. 23-29
Author(s):  
Mohammed Saad Mohammed Sherif ◽  
Ayman Mohammed Hagras ◽  
Mohammed Hassan Radwan ◽  
Mohammed Abo El-enen Khalwash
Keyword(s):  
Comparative Study ◽  
Testosterone Level ◽  
Serum Testosterone ◽  
University Hospital ◽  
Semen Parameters ◽  
Serum Testosterone Level ◽  
Male Population ◽  
Group A ◽  
The Mean ◽  
Group B

Introduction: Varicocele is a dilation of the pampiniform venous plexus and the internal Spermatic vein. It is detected approximately in 15% to 20 % of the general male Population; with the prevalence increasing to 40% in infertile men. Methodology: This prospective, comparative study was conducted on 134 male patients with primary infertility who attended the outpatient clinic of Urology Department, Tanta University Hospital during the period between April 2017 to April 2019. The patients were divided into two groups: Group A (56 patients) with low testosterone level. Group B (78 patients) with normal testosterone level. Results: In group A, the mean ± SD of testosterone level improved significantly to 2.93 ± 0.77 and 3.36 ± 0.86 after 3 and 6 months respectively (p1-value=0.001* and p2-value=0.001* respectively). In group B, the mean ± SD of testosterone level improved significantly to 6.22 ± 1.51 and 6.82 ± 2.25 after 3 and 6 months respectively (p1-value=0.026* and p2-value=0.001* respectively). Conclusion: varicocelectomy could improve semen parameters, serum testosterone level and pregnancy rate.

Ajwa Date Fruit Extract Ameliorates the Effects of Alcohol on Weight of Liver in Male Albino Rats

2021 ◽  
Vol 15 (7) ◽  
pp. 1810-1812
Author(s):  
Zafar Iqbal ◽  
Rifat Shamim ◽  
Nighat Ara ◽  
Fauzia Siraj ◽  
Attya Zaheer ◽  
...  
Keyword(s):  
Adult Male ◽  
Tap Water ◽  
Medical Institute ◽  
Control Group ◽  
Albino Rats ◽  
Distilled Water ◽  
Toxic Substances ◽  
Fruit Extract ◽  
Date Fruit ◽  
Group B

Background: Liver injuries induced by different toxic substances have been recognized as one of the great toxicological problem for years. However, there are a lot of herbal medicines existing to compensate these disorders.Alcohols are one of the most important organic compounds in different areas of our daily lives. The health benefits of Ajwa Date Fruit Extract are well documented in literature. Objective: To determine the effect of alcohol on weight of liver and possible protective role of Ajwa date fruit extract (ADFE) in adult male albino rats. Study Design:Experimental Study Place and Duration of Study: Department of Anatomy, Postgraduate Medical Institute, Lahore from 1st October 2018 to 31st March 2019. Methodology: Twenty four adult male albino rats were used in this research and divided into the 4 groups A, B, C and D having 6 rats in each group. Group A was designated as control group;group B received ethanol 3g/kg/day of 40% v/v prepared in distilled water; group C received ethanol and ADFE1g/kg/day and group D received ethanol and ADFE 2g/kg/day by oral gavage once daily for 28 days.The 100% pure alcohol was dissolved in distilled water for preparation of 40% alcohol. Dates palm (Phoenix dactylifera L.) fruits washed with tap water and removed the seeds. The extract of the date fruits made by adding distilled water to date fruit (3:1) and leaving for 48 hours in refrigerator (4°C). Results: The increase in mean weight of liver in group B on 29th day of experiment were statistically significant (p=0.001) in comparison with the control. The mean weight of liver was decreased significantly after 28 days of experiment in experimental groups C and D. Conclusions: Alcohol administration in the male albino rats significantly increase their weight of liver which was improved by the use of ADFE. Key words: Alcohol, Ajwa date fruit extract, Rats

scholarly journals Estimate the level of testosterone hormone using I-chroma technique in males treated with Royal jelly and honeybee

2021 ◽  
Vol 4 (1) ◽  
pp. 16-18
Author(s):  
Baqer Hassan ◽  
Zainab Fadhil Abbas
Keyword(s):  
Testosterone Level ◽  
Royal Jelly ◽  
Serum Testosterone ◽  
Treated Group ◽  
Serum Testosterone Level ◽  
Control Group ◽  
Short Term Treatment ◽  
Testosterone Levels ◽  
Natural Treatment ◽  
Result Show

The experiment based on 10 matured men samples collected randomly from Baghdad Providence. The 10 samples divided into two groups, serum testosterone level of all 10 patient had been done using Ichroma technique. the treated group administered 2.5ml of honeybee with 350mg of royal jelly daily for (15day) while the second group stay as control group. After 15 days blood samples had been collected and serum separated to made another testosterone test for both groups using same i-chroma technique. The main Aim of this study is estimating the effect of royal jelly and honeybee as food on the testosterone hormone level in men. The result show that there was significant increase in the testosterone levels in treated group in comparison with control group. after experiment the result show significant increase in testosterone levels (in samples number 1,2,3,4 and 5) as the following (23.2 n.mo/L, 16.6 n.mo/L, 17.7 n.mo/L, 10.1 n.mo/L and 29.7 n.mo/L respectively. The study concluded that, short term treatment of royal jelly in combined with honey bee increasing the level of blood testosterone hormone in men in different ages significantly. The study either concluded that we can used honeybee and royal jelly as natural treatment of low testosterone level in men.

scholarly journals Effect of monosodium glutamate on liver of adult albino rats: a light microscopic study

2021 ◽  
Vol 9 (5) ◽  
pp. 1442
Author(s):  
Prenika Shangloo ◽  
Midhat Syed ◽  
Sangeeta Gupta
Keyword(s):  
Dose Group ◽  
Low Dose ◽  
Monosodium Glutamate ◽  
Test Group ◽  
Control Group ◽  
Albino Rats ◽  
High Dose ◽  
Distilled Water ◽  
Group A ◽  
Group B

Background: Monosodium glutamate (MSG) or Aji-no-moto is the common flavouring agent which is inadvertently used in all the packed and ready to use food items. Its use has grabbed the attention with reporting of Chinese restaurant syndrome and many more side effects. This flavouring agent effects almost all the organs of the human body but the statistics regarding its ill effects are very limited, thus no objections are being raised for its use in eatables. In current study we planned to analyse the pathological effects of MSG on the liver of adult albino rats.Methods: The study was conducted on 18 inbred adult albino rats of either sex. The rats of control group (A) received only standard diet with distilled water, low dose test group (B) rats received 0.5 mg/kg of MSG dissolved in distilled water and high dose test group (C) rats received 1.5 mg/kg of MSG dissolved in distilled water per orally for 28 days. After the experimental period, the rats were sacrificed to dissect out the liver tissue which was later subjected to histological processing and tissue sectioning.Results: The liver tissue sections of the control group (A) revealed normal hepatic architecture with central veins located in the centre of the hepatic lobule and portal areas containing portal triad formed by portal venule, hepatic arteriole and bile ductile. On the other hand, the liver sections of low dose group (B) exhibited pathological changes in the form of dilated and congested central vein with sinusoidal dilatation. In high dose group (C), more marked pathological changes seen in group B along with dilatation of the portal vein was also seen.Conclusions: MSG is most widely used food additive whose safe limits for use need to be scrutinized. The current study was planned to access the minimal low dose limit of MSG for use. The observations of the afore mentioned study revealed that even small dose of MSG of 0.5 mg/kg is capable of producing pathological effects in liver which is the main site of metabolism of xenobiotics

scholarly journals Non-Surgical Castration in Bucks A Comparative Study between Chemical Castration and External Ligation of the Spermatic Cord

2012 ◽  
Vol 11 (1) ◽  
pp. 92
Author(s):  
R. N. Al-Asadi And Kh. K. Al-Kadi
Keyword(s):  
Testosterone Level ◽  
Spermatic Cord ◽  
Interstitial Fibrosis ◽  
Histopathological Examination ◽  
Sperm Concentration ◽  
Serum Testosterone ◽  
Semen Parameters ◽  
Serum Testosterone Level ◽  
Control Group ◽  
Chemical Castration

This study aimed to evaluate the effectiveness of intra-testicular injection of formalin and external ligation of the spermatic cord in inducing sterilization in bucks. Twelve's adult bucks were used which allocated randomly and equally into three groups. The first group served as a control group injected (5) ml distilled water intra-testicular, while the second group injected (5) ml of 3% formalin and in the third group external ligation of spermatic cord with silk was used.Blood samples were collected from all bucks prior and post to treatment to assay serum testosterone level. In addition, semen samples were collected via artificial vagina to evaluate some semen parameters.Clinical follow-up of animals revealed no any secondary complications in control group, while treatment group showed certain minor complications such as testicular swelling, hydrocele, scrotal ulcer and lameness which disappeared in a short time.Results indicated that testosterone level were significantly decline P<0.05 in the second and third groups, it reached to (0.30±0.05) and (1.66±0.25) ng/ml respectively at the end of experiment.All semen parameters were significantly P<0.05 decreased on days 21 and 28 in treatment groups, in which there was seminal plasma only, absence of sperm motility, zero sperm concentration and no viable sperms.All animals subjected to traditional castration on 30 and 60 days post-injection or ligation to harvested testicular biopsies for histopathological examination. Testicular sections revealed depletion of sertoli cells and interstitial fibrosis which replaced leydig cells. These changes were more severe in chemical group when compared with ligation group. This means that both techniques reflected their ability to impair or stop the testicular functions with superiority of chemical castration.

scholarly journals Association of Serum testosterone Level with Depression in Parkinson’s Disease

2014 ◽  
Vol 27 (2) ◽  
pp. 57-63
Author(s):  
Hasan Zahidur Rahman ◽  
Md. Rafiqul Islam ◽  
Abdus Salam ◽  
Md. Moniruzzaman Bhuiyan ◽  
Abu Nasir Rizvi ◽  
...  
Keyword(s):  
Testosterone Level ◽  
Serum Testosterone ◽  
Motor Symptom ◽  
Serum Testosterone Level ◽  
Control Group ◽  
Total Testosterone ◽  
Testosterone Deficiency ◽  
One Year ◽  
Medical University ◽  
Control Study

Background: Depression is a non-motor symptom in Parkinson’s disease (PD) as well as elderly population due to testosterone deficiency (TD). Because of similarity between depression in PD and for testosterone deficiency, clinician may fail to recognize and treat TD in patients with PD. Objectives: Our aim was to find out the association between serum total testosterone level and depression in men with Parkinson disease. Methods:This was a case control study carried out in the OPD of department of Neurology, Department of Psychiatry and Department of Biochemistry, Bangabandhu Sheikh Mujib Medical University (BSMMU) on total of 64 subjects where 34 were cases and 30 were controls age ranging from 40 to 85 years of age. Results: This analysis showed serum testosterone levels were significantly low in PD patients with depression compared to controls ( 4.90±1.86 ng/ml in cases and 6.66±1.18 ng/ml in control group with Odds ratio is 2.071; 95% CI 1.587-2.704). Significantly (P <0.05) low levels of serum testosterone were noted in case suffering from depression more than one year (4.56±1.82 ng/ml vs 6.18±1.50 ng/ml). Serum testosterone level was significantly (<0.001) low in stage III PD patients with depression compared to control (4.96±1.41 ng/ml vs 7.30±1.23 ng/ml). Conclusion: Low serum testosterone level is an important factor causing depression in PD patients. Also progression of PD and duration. Bangladesh Journal of Neuroscience 2011; Vol. 27 (2) : 57-63 DOI: http://dx.doi.org/10.3329/bjn.v27i2.17541

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