Estimate the level of testosterone hormone using I-chroma technique in males treated with   Royal jelly and honeybee

The experiment based on 10 matured men samples collected randomly from Baghdad Providence. The 10 samples divided into two groups, serum testosterone level of all 10 patient had been done using Ichroma technique. the treated group administered 2.5ml of honeybee with 350mg of royal jelly daily for (15day) while the second group stay as control group. After 15 days blood samples had been collected and serum separated to made another testosterone test for both groups using same i-chroma technique. The main Aim of this study is estimating the effect of royal jelly and honeybee as food on the testosterone hormone level in men. The result show that there was significant increase in the testosterone levels in treated group in comparison with control group. after experiment the result show significant increase in testosterone levels (in samples number 1,2,3,4 and 5) as the following (23.2 n.mo/L, 16.6 n.mo/L, 17.7 n.mo/L, 10.1 n.mo/L and 29.7 n.mo/L respectively. The study concluded that, short term treatment of royal jelly in combined with honey bee increasing the level of blood testosterone hormone in men in different ages significantly. The study either concluded that we can used honeybee and royal jelly as natural treatment of low testosterone level in men.

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Effect of Quinolones on Serum Testosterone Level in Male Albino Rats

Annals of King Edward Medical University ◽

10.21649/akemu.v23i1.1495 ◽

2017 ◽

Vol 23 (1) ◽

Author(s):

Rubina Iqbal ◽

Saud Iqbal ◽

Shahzad Anjum

Keyword(s):

Testosterone Level ◽

Morphological Changes ◽

Serum Testosterone ◽

Serum Testosterone Level ◽

Term Therapy ◽

Control Group ◽

Albino Rats ◽

Distilled Water ◽

Blood Samples ◽

Testosterone Levels

Objective: To determine the toxic effects of quinolones on serum testosterone level in male albino rats.Methods: Eighty male albino rats were randomly divided into A, B, C and D groups each group having 20 albino rats. These groups were further subdivided into A1, A2, B1, B2, C1, C2, D1 and D2 having 10 albino rats each. Ciprofloxcin, ofloxacin and enoxacin dissolved in distilled water were given at 135mg/kg/ day, 72mg/kg/day and 12.5mg/kg/day to groups A, B and C respectively for 12 weeks. Only distilled water was given to group D which was control group for the same time period. Blood samples were drawn for testosterone hormone level estimation at 0, 14th, 28th and 42nd day in subgroups Al, B1, Cl and D1 and then the animals in said groups were sacrificed on 42nd day to identify testicular morphological changes. Rats in subgroup A2, B2, C2 and D2 were kept alive till 84th day after stopping drugs at 42 days to find out if there is any change in levels of testosterone after discontinuation of the treatment. Blood samples for testosterone hormone estimation were taken at 0, 14th, 28th, 42nd, 56th, 70th and 84th day from subgroups A2, B2, C2 and D2. Testosterone hormone estimation assay was performed on the collected samples.Results: Testosterone assay showed significant decrease in serum testosterone levels in all experimental groups, when compared with control group. These levels did not return back to normal even after withdrawal of drugs. This study suggests a gonadotoxic potential of quinolones.Conclusion: Quinolones reduce serum testosterone levels and should be used carefully for long term therapy.

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Effects of curcumin on sperm parameters abnormalities induced by morphine in rat

Journal of Medical and Biomedical Sciences ◽

10.4314/jmbs.v6i2.1 ◽

2017 ◽

Vol 6 (2) ◽

pp. 1-10

Author(s):

S.H. Roshankhah ◽

M.R. Salahshoor ◽

S. Aryanfar ◽

F. Jalili ◽

M. Sohrabil ◽

...

Keyword(s):

Sperm Motility ◽

Testosterone Level ◽

Sperm Count ◽

Serum Testosterone ◽

Treated Group ◽

Sperm Parameters ◽

Male Rats ◽

Serum Testosterone Level ◽

Seminiferous Tubules ◽

Control Group

Opioids are the most potent and effective analgesics available and have become accepted as appropriate treatment for acute, cancer and non-cancer. Morphine, which is commonly used for the treatment of severe pain, gastrointestinal tract and kidneys. Curcumin petals consist of, glycosides, flavonoids, and anthocyanin. The study aims at evaluating curcumin effect and morphine on sperm parameters, testis tissue and serum testosterone level in rat. In this experimental study, 48 male rats with 28 weeks of age and limited weight of 270 to 300g were selected. They were divided into eight groups of 6, untreated control group; morphine – treated group (20 mg/kg/day); curcumin -treated groups (10, 30, 60 mg/kg/day); and morphine and curcumin treated group intraperitoneal administration for successive 28 days. After 24hours animals were killed. Sperm motility was measured using WHO protocols. The sperm parameter such as motility, sperm count, morphology, seminiferous tubules diameter, weight testis, and serum testosterone level were analyzed (oneway ANOVA). Curcumin (10, 30 and 60 mg/kg) significantly increased mean percentage of sperm motility, count, testis weight, and serum testosterone level compared to control group (p<0.05). Testosterone level decreased significantly in rats treated with morphine. Co-administration of curcumin to morphine-treated rats improved the histopathological alterations induced by morphine in testis and increased the sperm count. Curcumin has a very strong antioxidant effects at applied doses and it can probably be used as an antioxidant and food supplement in reproductive disorders.Journal of Medical and Biomedical Sciences (2017) 6(2), 1-10

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Vitamin C ameliorates the adverse effects of dexamethasone on sperm motility, testosterone level, and spermatogenesis indexes in mice

Human & Experimental Toxicology ◽

10.1177/0960327118816137 ◽

2018 ◽

Vol 38 (4) ◽

pp. 409-418 ◽

Cited By ~ 4

Author(s):

F Sadeghzadeh ◽

MS Mehranjani ◽

M Mahmoodi

Keyword(s):

Adverse Effects ◽

Vitamin C ◽

Sperm Motility ◽

Testosterone Level ◽

Leydig Cells ◽

Serum Testosterone ◽

Serum Testosterone Level ◽

Control Group ◽

The Mean ◽

Vit C

Background: Dexamethasone (DEX) is a common medicine that is capable of causing malformation in the male reproductive system. The aim of this study was to investigate the effect of vitamin C (Vit-C) on spermatogenesis indexes and daily sperm production (DSP) in adult mice treated with DEX. Methods: Male Naval Medical Research Institute (NMRI) mice were divided into four groups: Control, DEX (7 mg/kg/day), Vit-C (100 mg/kg/day), and DEX +Vit-C and treated for 7 days with intraperitoneal injection. Results: A significant increase in the mean levels of serum and tissue malondialdehyde (MDA) and apoptosis of Leydig cells was found in the DEX group compared to the control group. Sperm motility, DSP, tubular differentiation index, meiotic index, spermatogenesis index, the mean number of spermatocytes, round and long spermatids, and Leydig cells, and also serum testosterone level decreased in the DEX group compared to the control group. The results of this study indicate that Vit-C can significantly prevent the adverse effects of DEX on the mean number of spermatocyte, spermatid, and Leydig cells, tubular differentiation, meiotic and spermatogenesis index, DSP, sperm motility, and the mean levels of serum MDA. Conclusion: In conclusion, our results showed that coadministration of Vit-C and DEX prevents the adverse effects of DEX on the spermatogenesis indexes and DSP.

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Impact of Varicocele and Varicocele Surgery on Semen Quality, Erectile Function and Serum Hormone Levels

Current Opinion in Gynecology and Obstetrics ◽

10.18314/cogo.v1i1.1431 ◽

2018 ◽

Vol 1 (1) ◽

pp. 129-136

Author(s):

Badereddin Mohamad Al-Ali ◽

Emma Persad ◽

Andreas Lunacek ◽

Christof Mrstik ◽

Eugen Plas

Keyword(s):

Testosterone Level ◽

Erectile Function ◽

Semen Quality ◽

Semen Analysis ◽

Serum Testosterone ◽

T Test ◽

Serum Testosterone Level ◽

Testosterone Levels ◽

Group 2 ◽

Group 1

Introduction: Many studies suggest that varicoceles are associated with hypogonadism and varicocele repair can increase testosterone levels and improve erectile function.Aim: The aim of this retrospective study was to analyze the impact of varicocele and varicocele surgery on testosterone level, semen quality, and erectile function.Methods: Our study included 265 infertile males with a clinical varicocele. This group was divided into three groups: group 1 (193) patients who did not receive surgery, group 2 (72 patients) who were operated on according to the Palomo procedure and group 3 (28 patients), who acted as a control group without a varicocele. All patients completed the International Index of Erectile Function IIEF-5 (German version) and underwent semen analysis. Serum testosterone, follicle stimulating hormone (FSH), and luteinizing hormone (LH) were measured at inclusion into the study and after surgery.Main outcome measures: Changes in semen quality, erectile function, and serum hormones after surgery.Results: The IIEF-5 scores in groups 1 and 2 were 21.01 ± 2.2; and 21.74 ± 1 respectively, and the resulting t-test for equality of variance was significant (p < 0.0001). Total testosterone level in groups 1 and 2 were 3.16 ± 0.37 and 3 ± 0.01, respectively, and the resulting t-test for equality of variance was significant (p < 0.0001). The results of the semen analysis were better in group 2 (after surgery) (28.6%, p < 0.001) in comparison to group 1. Interestingly, pre-operative serum testosterone levels were lower in patients with later improvement of semen analysis (p = 0.05). Body mass index (p = 0.8), pre-operative serum FSH (p = 0.9), LH (p = 0.2), and nicotine consumption (p = 0.6) were similar in both the group that saw improvement and the group with no change in semen quality.Conclusion: Semen quality improved in 28.6% of patients after varicocele surgery. Erectile dysfunction (ED) improved after varicocele surgery. We report that lower pre-operative serum testosterone level might be a possible indicator for successful surgical outcome.

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Serum testosterone level and overall survival in first-line abiraterone and enzalutamide for metastatic castration-resistant prostate cancer patients.

Journal of Clinical Oncology ◽

10.1200/jco.2020.38.15_suppl.e17545 ◽

2020 ◽

Vol 38 (15_suppl) ◽

pp. e17545-e17545

Author(s):

Maysa Tamara Silveira Vilbert ◽

Marcelle Goldner Cesca ◽

Natasha Carvalho Pandolfi ◽

Vinicius Fernando Calsavara ◽

Bruno Cezar de Mendonça Uchôa ◽

...

Keyword(s):

Prostate Cancer ◽

Overall Survival ◽

Testosterone Level ◽

Serum Testosterone ◽

Serum Testosterone Level ◽

Castration Resistant Prostate Cancer ◽

First Line ◽

Testosterone Levels ◽

Castration Resistant ◽

Low Serum

e17545 Background: Androgen receptor-targeted agents Abiraterone and Enzalutamide (Abi/Ez) prolonged overall survival in metastatic castration resistant prostate cancer (mCRPC). Patients with very-low serum testosterone levels seem to have less benefit from these therapies as well as more aggressive prostate cancer. Methods: A retrospective observational cohort study was conducted to evaluate whether a serum testosterone measured at time of start first-line therapy with Abi/Ez is related to overall survival (OS) and time-to-treatment failure (TTF) in mCRPC patients. Kaplan-Meier survival estimates and Cox-regression models were used for time-to-event analyses. The best cut-off for testosterone was defined using Log-rank statistics (Lausen and Schumacher). X² test and Mann-Whitney U-test were applied to compare categorical and continuous variables, respectively. Logistic regression was used to assess characteristics related to serum testosterone levels. Statistical significance was fixed at 0.05. Results: From May 2012 to February 2017, 100 patients were assessed. Median follow-up was 27.8 months (range 2.23 to 68.26). Pts with a high testosterone level ( > 28.2; n = 20) achieved a significantly higher OS (median 66.0 vs 31.9 mo, testosterone > 28.2 HR: 0.206, 95% CI 0.074 to 0.571, p = 0.002) and TTF (median 30.6 vs 11.8 mo, testosterone > 28.2 HR: 0.408, 95%CI 0.219 to 0.762, p = 0.005) than pts with a low serum testosterone level ( < 28.2; n = 80), regardless of receiving therapy with either Abi (n = 69) or Ez (n = 31). Pts with a higher testosterone level were younger (median 67.7 vs 73.6 years; p = 0.026), had a higher body mass index (BMI) (28.5 vs 25.9, p = 0.023) and a lower PSA at start Abi/Ez (12 vs 26, p = 0.031) than pts with lower values. Age (OR 0.93, 95%CI 0.8 to 0.9, p = 0.021), BMI (OR 1.21, 95%CI 1.1 to 1.4, p = 0.006) and baseline PSA (OR 1.2, 95%CI 1.03 to 1.4, p = 0.020) were significantly associated with testosterone > 28.2. After 4 months of Abi/Ez treatment, PSA decrease > 50% of baseline was seen more frequently in high testosterone levels group than in low testosterone levels pts (90% vs 57.5% of pts, respectively, p = 0.007). Conclusions: Pts with high levels of testosterone ( > 28.2) achieved a better OS and TTF when treated with Abi/Ez in first-line mCRPC than those with low levels. Testosterone can be considered a prognostic and predictive biomarker in this scenario, and could be used in treatment decision for this population.

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Percutaneous orchiectomy: A noninvasive method of castration beyond percutaneous testicular sclerosis

Journal of Clinical Oncology ◽

10.1200/jco.2009.27.15_suppl.e16153 ◽

2009 ◽

Vol 27 (15_suppl) ◽

pp. e16153-e16153

Author(s):

E. C. Nepomuceno ◽

F. Quintiliano ◽

F. S. Lima ◽

E. Café ◽

P. Boente

Keyword(s):

Prostate Cancer ◽

Serum Testosterone ◽

Serum Levels ◽

Serum Testosterone Level ◽

Control Group ◽

Ischemic Lesion ◽

Alcohol Injection ◽

Percutaneous Injection ◽

Testosterone Levels ◽

Percutaneous Administration

e16153 Background: Surgical castration is the gold standard for hormonal deprivation in metastatic prostate cancer, nevertheless this simple procedure may involve on psychological consequences. According to many studies, it's possible to achieve ischemic lesion in liver tissue beyond sclerosants agents (like alcohol or glycerol), however there are very few reports about the effects of such agents in testicles. These study objectives evaluating histological and morphological characteristics of rat testicles submitted to percutaneous administration of sclerosants agents and also, to compare serum testosterone levels between rats submitted to a surgical orchiectomy or percutaneous injection. Methods: Twenty four rats have been shared in four groups with eight animals each. In group O, rats were submitted to bilateral orchiectomy. In the other groups, rats were submitted to percutaneous administration of a sclerosant agent and orquiectomy after thirty days as follows: Group A, Alcohol injection; Group G - Glycerol; Group S - Saline solution (control group). Serum testosterone level was measured after 15 and 30 days in each animal. Results: There is no complication or death in this series. Rats of groups A and G comparing to control group (group S) had smaller testicular weight (0,8±0,1g; 1±0,2g versus 3,15±0,1g p<0,0000001) and smaller testicular volume (0,16±0,05mL; 0,23±0,11mL versus 2,38±0,05mL p<0,0000001). Testosterone serum levels were as similar in groups A and G (sclerosis) as in group O (orchiectomy). After 15 days testosterone levels were A=2,9±0,74 ng\dL; G=2,8±0,39 ng\dL versus O=2,91±1,46ng\dL p=0,99; and after 30 days were A=2,58±0,4ng\mL, G=2,78±0,3ng\mL versus O=2,7±0,95ng\mL p=0,895). Histological findings show extensive necrosis beyond macrophagic infiltration and no Leydig cells visualized.There is no significantly statistical difference between Alcohol and Glycerol groups. Conclusions: Percutaneous administration of alcohol or glycerol in rats testicles causes atrophy and reduces testosterone serum levels like it occurs after surgical castration. More studies are necessary to evaluate if this minimally invasive procedure may be an alternative to surgical orchiectomy in advanced prostate cancer. No significant financial relationships to disclose.

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Prepubertal Serum Testosterone Concentration In Vembur Rams At Different Rearing System

THE INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY ◽

10.21887/ijvsbt.v12i4.7687 ◽

2017 ◽

Vol 12 (4) ◽

Author(s):

R. Selvakkumar ◽

T. Sivakumar ◽

S. Ganesh Kumar ◽

P. Anuradha

Keyword(s):

Field Condition ◽

Testosterone Level ◽

Serum Testosterone ◽

Serum Testosterone Level ◽

Testosterone Concentration ◽

Testosterone Levels ◽

Ram Lambs ◽

Age At Puberty

The present investigation was carried out to study the prepubertal testosterone pattern and age at puberty in Vembur rams reared under farm and farmers field condition. A total number of 8 Vembur ram lambs each from farm and farmers field were selected. The measurement of testosterone was performed by Radioimmunoassay (RIA). The serum testosterone level from 6th to 20th month of age ranged from 0.132 ± 0.04 to 3.823 ng/ml in semi intensively maintained farm males and 0.138±0.04 to 3.456±0.17 ng/ml in animals maintained at farmer’s holdings. The serum testosterone levels were crossing the concentration of 2.5 ng/ml at the age of 17 to 18th weeks and successful mounting, intromission and ejaculation were noticed after this age.

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Evaluate the Effect of Amlodipine Besylate on Testosterone and Fertility of Male albino Rats

Pakistan Journal of Medicine and Dentistry ◽

10.36283/pjmd9-3/004 ◽

2020 ◽

Author(s):

Shahid Ali

Keyword(s):

Testosterone Level ◽

Channel Blocker ◽

Serum Testosterone ◽

Serum Testosterone Level ◽

Control Group ◽

Albino Rats ◽

Distilled Water ◽

Ca Channel ◽

Amlodipine Besylate ◽

Group B

Background: Amlodipine is a Ca++ channel blocker, routinely used for treatment of hypertension. The mechanism underlying its anti-hypertensive effect is dilatation of blood vessels. Testosterone, the hormone produced by testis, is responsible for development of male sexual secondary characteristics. The aim of study was to find out the effect of Ca++ channel blocker, Amlodipine besylate on serum testosterone level in male albino Rats. Methods: Duration of study was 30 days and conducted in department of pharmacology, 24 male Albino rats were grouped into 4 groups (each having 6). Group A was control group, Group B, C and D were treated with Amlodipine Besylate drug at doses 0.07, 0.14 and 0.28mg/kg body weight respectively (Suspension of 5 mg tablet of Amlodipine dissolved in 10 ml distilled water prepared and further diluted to 1:10ml with distilled water). Independent t test and ANOVA with Tukey’s test were used for comparison of quantitative variables with in groups. Results: Serum testosterone level reduced in groups treated with Amlodipine. Serum testosterone level of control group is 11.22 while group B, C and D were 4.7, 2.6 and 0.7 respectively. Serum FSH level of control group is 0.10±0.01 while group B, C and D were 0.24±0.35, 0.34±0.40 and 0.08±0.03 respectively. It showed that increasing the dose of Amlodipine markedly reduce testosterone level and increases FSH level in male albino Rats. Conclusion: Amlodipine severely reduce level of serum testosterone (p=0.001) and increases FSH level (p=0.305) which can lead to infertility in male albino Rats.

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Testosterone therapy: Prescribing and monitoring patterns of practice in British Columbia

Canadian Urological Association Journal ◽

10.5489/cuaj.6586 ◽

2020 ◽

Vol 15 (2) ◽

Author(s):

Jennifer A. Locke ◽

Ryan Flannigan ◽

Oliver P. Günther ◽

Sean Skeldon ◽

S. Larry Goldenberg

Keyword(s):

British Columbia ◽

Testosterone Level ◽

Serum Testosterone ◽

Population Based ◽

Further Education ◽

Serum Testosterone Level ◽

Dose Titration ◽

Testosterone Therapy ◽

Testosterone Levels ◽

Prescribing Practice

Introduction: Guidelines recommend that testosterone therapy (TTh) be restricted to men with a biochemical diagnosis of hypogonadism, and that therapeutic responses be titrated within the normal range. Methods: Using four provincial longitudinal databases in British Columbia, we identified men prescribed TTh (1997–2013). We characterized the prescribing and monitoring practices of TTh in the context of serum testosterone levels drawn prior to and following initiation of TTh in a population-based setting. Results: In our analysis of 37 741 men who received at least one TTh prescription, 48% received injectable testosterone and the vast majority were treated by general practitioners. The number of prescriptions for men increased annually, particularly after 2008; 40% discontinued their treatment after their first or second prescription, while 27% received more than 10 repeats. The absolute percentage of pre- and post-serum testosterone levels ordered increased by 16% and 31% during the study period, respectively. However, after initiating TTh, only 36% of all the men had a followup serum testosterone level drawn. Of those with low serum testosterone levels prior to TTh, 49% remained biochemically hypogonadal following TTh, suggesting non-compliance or inadequate dosing. Conclusions: Many men prescribed TTh did not continue beyond a short trial. While the practice of checking pre-and post-TTh testosterone levels has improved over the study period, it is concerning that only one-third had a followup serum testosterone level, and half remained biochemically low. Further education is required around TTh prescribing, dose titration, and monitoring to ensure both effective and safe prescribing practice.

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Effect of oral injection of tramadol, sildenafil and combined of tramadol and sildenafil on serum testosterone level for Wistar breed male rats

INTERNATIONAL JOURNAL OF COMPUTERS & TECHNOLOGY ◽

10.24297/ijct.v11i9.3411 ◽

2013 ◽

Vol 11 (9) ◽

pp. 2959-2966

Author(s):

Rabie Said Farag ◽

Mohamed Abdel El Nawawy ◽

Wael Mohamed Fathy ◽

Hamada Atiaa

Keyword(s):

Erectile Dysfunction ◽

Serum Testosterone ◽

Water Soluble ◽

Pharmacological Effect ◽

Male Rats ◽

Absolute Bioavailability ◽

Serum Testosterone Level ◽

Control Group ◽

Testosterone Levels ◽

The Mean

Introduction:Testosterone is the main sexual hormone in human males, and has a pharmacological effect on the physiology of sexual function. It is known that suppression of testosterone in eugonadal adult males leads to reduced sexual desire and activity, and may result in erectile dysfunction. Tramadol is rapidly and almost completely absorbed after oral administration but its absolute bioavailability is only 65â€“70% due to first-pass metabolism. Sildenafil citrate is a water soluble citrate salt that was firstly synthesized by Pfizer in United Kingdom to treat hypertension and angina pectoris. Interestingly, this drug exhibited a different pharmacological effect, a marked penile erection, and became the first-line treatment option to erectile dysfunction. Aim of the work :the main work goal of this search was to measure and compare between mean serum testosterone concentrations after oral injection of tramadol, sildenafil , and combination of tramadol and sildenafil beside healthy subjects.Investigated Parameter: quantitative analysis of Free Testosterone ELISA Kit in serum by The Calbiotech, Inc. 10461 Austin Dr, Spring Valley, CA U.S.A., depending on McCann, D., et al 1985.Results In the present study, the results of GT group revealed that administration of tramadol 50 mg /Kg/day for 10, 20 days and 30 days indicated that the measured testosterone levels were lower than the (GC) control group, and the decrease in testosterone mean concentrations in GT3, than GT2, than GT1. In our research GS group administration of sildenafil 50 mg /Kg/day for 10, 20 and 30 days groups indicated that increase testosterone levels than that in control GC group, and there was an increase in testosterone mean concentrations in GS3, than GS2, than GS1. The present study reveled in GT&S administration of tramadol 50 mg /Kg/day combined with sildenafil 50 mg /Kg/day that the mean serum testosterone concentrations in (GT&S1), (GT&S2) and (GT&S3), were decrease than in the mean serum testosterone concentrations (GC), but this decrease in the mean serum testosterone concentrations in (GT&S1), (GT&S2) and (GT&S3), were quite higher than mean serum testosterone concentrations in GT1, GT2, and GT3, also and there were a decrease in mean testosterone mean concentrations in (GT&S3) than that in (GT&S2) and (GT&S1)conclusion: tramadol administration decrease testosterone levels, while sildenafil increase testosterone levels, while the combination between tramadol & sildenafil decrease testosterone levels.

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