scholarly journals Effect of oral injection of tramadol, sildenafil and combined of tramadol and sildenafil on serum testosterone level for Wistar breed male rats

2013 ◽  
Vol 11 (9) ◽  
pp. 2959-2966
Author(s):  
Rabie Said Farag ◽  
Mohamed Abdel El Nawawy ◽  
Wael Mohamed Fathy ◽  
Hamada Atiaa
Keyword(s):  
Erectile Dysfunction ◽  
Serum Testosterone ◽  
Water Soluble ◽  
Pharmacological Effect ◽  
Male Rats ◽  
Absolute Bioavailability ◽  
Serum Testosterone Level ◽  
Control Group ◽  
Testosterone Levels ◽  
The Mean

Introduction:Testosterone is the main sexual hormone in human males, and has a pharmacological effect on the physiology of sexual function. It is known that suppression of testosterone in eugonadal adult males leads to reduced sexual desire and activity, and may result in erectile dysfunction. Tramadol is rapidly and almost completely absorbed after oral administration but its absolute bioavailability is only 65–70% due to first-pass metabolism. Sildenafil citrate is a water soluble citrate salt that was firstly synthesized by Pfizer in United Kingdom to treat hypertension and angina pectoris. Interestingly, this drug exhibited a different pharmacological effect, a marked penile erection, and became the first-line treatment option to erectile dysfunction. Aim of the work :the main work goal of this search was to measure and compare between mean serum testosterone concentrations after oral injection of tramadol, sildenafil , and combination of tramadol and sildenafil beside healthy subjects.Investigated Parameter: quantitative analysis of Free Testosterone ELISA Kit in serum by The Calbiotech, Inc. 10461 Austin Dr, Spring Valley, CA U.S.A., depending on McCann, D., et al 1985.Results In the present study, the results of GT group revealed that administration of tramadol 50 mg /Kg/day for 10, 20 days and 30 days indicated that the measured testosterone levels were lower than the (GC) control group, and the decrease in testosterone mean concentrations in GT3, than GT2, than GT1. In our research GS group administration of sildenafil 50 mg /Kg/day for 10, 20 and 30 days groups indicated that increase testosterone levels than that in control GC group, and there was an increase in testosterone mean concentrations in GS3, than GS2, than GS1. The present study reveled in GT&S administration of tramadol 50 mg /Kg/day combined with sildenafil 50 mg /Kg/day that the mean serum testosterone concentrations in (GT&S1), (GT&S2) and (GT&S3), were decrease than in the mean serum testosterone concentrations (GC), but this decrease in the mean serum testosterone concentrations in (GT&S1), (GT&S2) and (GT&S3), were quite higher than mean serum testosterone concentrations in GT1, GT2, and GT3, also and there were a decrease in mean testosterone mean concentrations in (GT&S3) than that in (GT&S2) and (GT&S1)conclusion: tramadol administration decrease testosterone levels, while sildenafil increase testosterone levels, while the combination between tramadol & sildenafil decrease testosterone levels.

Toxicological evaluation of hydroethanol leaf extract of Pupalia lappacea (Linn.) Juss. (Amaranthaceae) in rodents

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Murtala Akanji Abdullahi ◽  
Elijah Oladapo Oyinloye ◽  
Akinyinka Alabi ◽  
Aderonke Adeyinka Aderinola ◽  
Luqman Opeyemi Ogunjimi ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Density Lipoprotein ◽  
Serum Testosterone ◽  
Female Rats ◽  
Male Rats ◽  
Laboratory Animals ◽  
Serum Testosterone Level ◽  
Control Group ◽  
Toxicological Evaluation ◽  
Liver And Kidney

Abstract Objectives Several studies have established the ethnobotanical benefits of Pupalia lappacea (PL) in laboratory animals without extensive toxicological evaluation of its safety profiles. Thus, an extensive toxicological investigation of sub-chronic oral administration of the hydroethanol leaf extract of P. lappacea in rodents was carried out in this study. Methods Different groups of rats were treated orally with the extract (10, 50 and 250 mg/kg) daily for 90 consecutive days. The control group received distilled water (10 mL/kg). After 90 days, some rats were left for additional 30 days without treatment for reversibility study. Blood and organs samples were collected for different evaluations at the end of study periods. Results The extract decreased the bodyweights, feeding and water intakes in female rats. PL increased the weights of the liver and kidney in male rats. PL increased the red blood cell (RBC), packed cell volume (PCV), hemoglobin (Hb), triglycerides (TRIG), cholesterol and high density lipoprotein (HDL) contents in rats. PL (250 mg/kg) significantly reduced the sperm motility and serum testosterone level. Cyto-architectural distortions of the testes, liver and spleen were visible. Conclusions The findings showed that P. lappacea is relatively safe at lower doses but cautions should be taken at higher dose.

Vitamin C ameliorates the adverse effects of dexamethasone on sperm motility, testosterone level, and spermatogenesis indexes in mice

2018 ◽  
Vol 38 (4) ◽  
pp. 409-418 ◽  
Author(s):  
F Sadeghzadeh ◽  
MS Mehranjani ◽  
M Mahmoodi
Keyword(s):  
Adverse Effects ◽  
Vitamin C ◽  
Sperm Motility ◽  
Testosterone Level ◽  
Leydig Cells ◽  
Serum Testosterone ◽  
Serum Testosterone Level ◽  
Control Group ◽  
The Mean ◽  
Vit C

Background: Dexamethasone (DEX) is a common medicine that is capable of causing malformation in the male reproductive system. The aim of this study was to investigate the effect of vitamin C (Vit-C) on spermatogenesis indexes and daily sperm production (DSP) in adult mice treated with DEX. Methods: Male Naval Medical Research Institute (NMRI) mice were divided into four groups: Control, DEX (7 mg/kg/day), Vit-C (100 mg/kg/day), and DEX +Vit-C and treated for 7 days with intraperitoneal injection. Results: A significant increase in the mean levels of serum and tissue malondialdehyde (MDA) and apoptosis of Leydig cells was found in the DEX group compared to the control group. Sperm motility, DSP, tubular differentiation index, meiotic index, spermatogenesis index, the mean number of spermatocytes, round and long spermatids, and Leydig cells, and also serum testosterone level decreased in the DEX group compared to the control group. The results of this study indicate that Vit-C can significantly prevent the adverse effects of DEX on the mean number of spermatocyte, spermatid, and Leydig cells, tubular differentiation, meiotic and spermatogenesis index, DSP, sperm motility, and the mean levels of serum MDA. Conclusion: In conclusion, our results showed that coadministration of Vit-C and DEX prevents the adverse effects of DEX on the spermatogenesis indexes and DSP.

Percutaneous orchiectomy: A noninvasive method of castration beyond percutaneous testicular sclerosis

2009 ◽  
Vol 27 (15_suppl) ◽  
pp. e16153-e16153
Author(s):  
E. C. Nepomuceno ◽  
F. Quintiliano ◽  
F. S. Lima ◽  
E. Café ◽  
P. Boente
Keyword(s):  
Prostate Cancer ◽  
Serum Testosterone ◽  
Serum Levels ◽  
Serum Testosterone Level ◽  
Control Group ◽  
Ischemic Lesion ◽  
Alcohol Injection ◽  
Percutaneous Injection ◽  
Testosterone Levels ◽  
Percutaneous Administration

e16153 Background: Surgical castration is the gold standard for hormonal deprivation in metastatic prostate cancer, nevertheless this simple procedure may involve on psychological consequences. According to many studies, it's possible to achieve ischemic lesion in liver tissue beyond sclerosants agents (like alcohol or glycerol), however there are very few reports about the effects of such agents in testicles. These study objectives evaluating histological and morphological characteristics of rat testicles submitted to percutaneous administration of sclerosants agents and also, to compare serum testosterone levels between rats submitted to a surgical orchiectomy or percutaneous injection. Methods: Twenty four rats have been shared in four groups with eight animals each. In group O, rats were submitted to bilateral orchiectomy. In the other groups, rats were submitted to percutaneous administration of a sclerosant agent and orquiectomy after thirty days as follows: Group A, Alcohol injection; Group G - Glycerol; Group S - Saline solution (control group). Serum testosterone level was measured after 15 and 30 days in each animal. Results: There is no complication or death in this series. Rats of groups A and G comparing to control group (group S) had smaller testicular weight (0,8±0,1g; 1±0,2g versus 3,15±0,1g p<0,0000001) and smaller testicular volume (0,16±0,05mL; 0,23±0,11mL versus 2,38±0,05mL p<0,0000001). Testosterone serum levels were as similar in groups A and G (sclerosis) as in group O (orchiectomy). After 15 days testosterone levels were A=2,9±0,74 ng\dL; G=2,8±0,39 ng\dL versus O=2,91±1,46ng\dL p=0,99; and after 30 days were A=2,58±0,4ng\mL, G=2,78±0,3ng\mL versus O=2,7±0,95ng\mL p=0,895). Histological findings show extensive necrosis beyond macrophagic infiltration and no Leydig cells visualized.There is no significantly statistical difference between Alcohol and Glycerol groups. Conclusions: Percutaneous administration of alcohol or glycerol in rats testicles causes atrophy and reduces testosterone serum levels like it occurs after surgical castration. More studies are necessary to evaluate if this minimally invasive procedure may be an alternative to surgical orchiectomy in advanced prostate cancer. No significant financial relationships to disclose.

scholarly journals Estimate the level of testosterone hormone using I-chroma technique in males treated with Royal jelly and honeybee

2021 ◽  
Vol 4 (1) ◽  
pp. 16-18
Author(s):  
Baqer Hassan ◽  
Zainab Fadhil Abbas
Keyword(s):  
Testosterone Level ◽  
Royal Jelly ◽  
Serum Testosterone ◽  
Treated Group ◽  
Serum Testosterone Level ◽  
Control Group ◽  
Short Term Treatment ◽  
Testosterone Levels ◽  
Natural Treatment ◽  
Result Show

The experiment based on 10 matured men samples collected randomly from Baghdad Providence. The 10 samples divided into two groups, serum testosterone level of all 10 patient had been done using Ichroma technique. the treated group administered 2.5ml of honeybee with 350mg of royal jelly daily for (15day) while the second group stay as control group. After 15 days blood samples had been collected and serum separated to made another testosterone test for both groups using same i-chroma technique. The main Aim of this study is estimating the effect of royal jelly and honeybee as food on the testosterone hormone level in men. The result show that there was significant increase in the testosterone levels in treated group in comparison with control group. after experiment the result show significant increase in testosterone levels (in samples number 1,2,3,4 and 5) as the following (23.2 n.mo/L, 16.6 n.mo/L, 17.7 n.mo/L, 10.1 n.mo/L and 29.7 n.mo/L respectively. The study concluded that, short term treatment of royal jelly in combined with honey bee increasing the level of blood testosterone hormone in men in different ages significantly. The study either concluded that we can used honeybee and royal jelly as natural treatment of low testosterone level in men.

scholarly journals Long-Term Efficacy and Tolerability of Abdominal Once-Yearly Histrelin Acetate Subcutaneous Implants in Patients with Advanced Prostate Cancer

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Sean Woolen ◽  
Cameron Holzmeyer ◽  
Emily Nesbitt ◽  
Paul F. Siami
Keyword(s):  
Prostate Cancer ◽  
Advanced Prostate Cancer ◽  
Serum Testosterone ◽  
Serum Testosterone Level ◽  
Serious Adverse Events ◽  
Testosterone Levels ◽  
Administration Method ◽  
The Mean ◽  
Androgen Independent

Objectives.Long-term assessment of the efficacy and tolerability of subcutaneous abdominal histrelin acetate implants that have been inserted for more than two years.Materials and Methods.Retrospective data collected over a six-year period at a single center from charts of 113 patients who received the subcutaneous abdominal histrelin acetate implant.Results.Following insertion of the first implant, 92.1% and 91.8% of patients had a serum testosterone level of ≤30 ng/dL at 24 and 48 weeks, respectively. Serum testosterone levels remained at <30 ng/dL for 96% of patients at two years and for 100% of patients at 3, 4, and 5 years. The testosterone levels remained significantly less than baseline(P<0.05). Six patients (5.3%) had androgen-independent progression when followed up on the long term, increasing the mean serum PSA at 3, 4, and 5 years to 35.0 µg/L(n=22), 30.7 µg/L(n=13), and 132.9 µg/L(n=8), respectively. The mean serum PSA was significantly greater than baseline during these years(P<0.05). Eight patients (7.1%) experienced minor, but not serious, adverse events from the histrelin acetate.Conclusion.Subcutaneous abdominal histrelin acetate implants are an effective long-term and well-tolerated administration method for treating patients with advanced prostate cancer.

scholarly journals Effect of Quinolones on Serum Testosterone Level in Male Albino Rats

2017 ◽  
Vol 23 (1) ◽  
Author(s):  
Rubina Iqbal ◽  
Saud Iqbal ◽  
Shahzad Anjum
Keyword(s):  
Testosterone Level ◽  
Morphological Changes ◽  
Serum Testosterone ◽  
Serum Testosterone Level ◽  
Term Therapy ◽  
Control Group ◽  
Albino Rats ◽  
Distilled Water ◽  
Blood Samples ◽  
Testosterone Levels

<p><strong>Objective:</strong><strong>  </strong>To determine the toxic effects of quinolones on serum testosterone level in male albino rats.</p><p><strong>Methods:</strong><strong>  </strong>Eighty male albino rats were randomly divided into A, B, C and D groups each group having 20 albino rats. These groups were further subdivided into A1, A2, B1, B2, C1, C2, D1 and D2 having 10 albino rats each. Ciprofloxcin, ofloxacin and enoxacin dissolved in distilled water were given at 135mg/kg/ day, 72mg/kg/day and 12.5mg/kg/day to groups A, B and C respectively for 12 weeks. Only distilled water was given to group D which was control group for the same time period. Blood samples were drawn for testosterone hormone level estimation at 0, 14<sup>th</sup>, 28<sup>th</sup> and 42<sup>nd</sup> day in subgroups Al, B1, Cl and D1 and then the animals in said groups were sacrificed on 42<sup>nd</sup> day to identify testicular morphological changes. Rats in subgroup A2, B2, C2 and D2 were kept alive till 84<sup>th</sup> day after stopping drugs at 42 days to find out if there is any change in levels of testosterone after discontinuation of the treatment. Blood samples for testosterone hormone estimation were taken at 0, 14<sup>th</sup>, 28<sup>th</sup>, 42<sup>nd, </sup>56<sup>th</sup>, 70<sup>th</sup> and 84<sup>th</sup> day from subgroups A2, B2, C2 and D2. Testosterone hormone estimation assay was performed on the collected samples.</p><p><strong>Results:</strong><strong>  </strong>Testosterone assay showed significant decrease in serum testosterone levels in all experimental groups, when compared with control group. These levels did not return back to normal even after withdrawal of drugs. This study suggests a gonadotoxic potential of quinolones.</p><p><strong>Conclusion:  </strong>Quinolones reduce serum testosterone levels and should be used carefully for long term therapy.<strong></strong></p>

scholarly journals COVID-19 may cause Erectile Dysfunction (ED) and the high level of testosterone is associated with poor prognosis

2021 ◽  
Author(s):  
kadir karkin ◽  
ergün alma ◽  
Hakan Erçil ◽  
keremhan gözükara ◽  
ferhat ortaoğlu ◽  
...  
Keyword(s):  
Intensive Care ◽  
Erectile Dysfunction ◽  
Testosterone Level ◽  
Age Groups ◽  
Serum Testosterone ◽  
Serum Testosterone Level ◽  
Age Group ◽  
Testosterone Levels ◽  
High Level ◽  
The Relationship

We aimed to investigate the relationship between COVID-19 and Erectile Dysfunction (ED) and the effect of serum testosterone level on the disease prognosis. Between April-December 2020, 70 patients who admitted with a complaint of ED after having COVID-19 and whose serum testosterone level was checked for any reason before COVID-19. The patients filled the International Index of Erectile Function (IIEF-5) and their testosterone level was checked. This questionnaire was arranged to present the first month before COVID-19 and after COVID-19. The patients were registered as 20-40 age group 1, 40-60 age group 2 and 60 years and above group 3. Testosterone levels of the patients before and after COVID-19 were compared. The relationship between testosterone levels and hospitalization in the intensive care was evaluated. It was revealed that testosterone levels and IIEF-5 scores after COVID-19 in all three groups were statisticaly and significantly different compared to the period before COVID-19 (p <0.05). Testosterone levels of patients in need of intensive care were significantly higher than those without any need of intensive care (p <0.05). Our study has presented that COVID-19 may cause ED and high testosterone levels increase the rate of hospitalization in the intensive care by intensifying the disease. Keywords COVID-19, erectile dysfunction, testosterone What’s already known about this topic? The relationship between Erectile Dysfunction (ED) and COVID-19 develops due to vasculogenic and hormonal causes which were caused by the primary disease. What does this article add? We showed with this study that COVID-19 causes ED in all age groups, reduces testosterone levels seriously. Moreover, we also presented that the higher the testosterone levels during COVID-19, the more severe the disease progresses.

scholarly journals Examination of Apoptotic and Autophagic Effects of Chronic Roflumilast Use on Rat Testicular Tissue by Immunohistochemical and Immunofluorescence Methods

2021 ◽  
Author(s):  
arzu gezer ◽  
Ebru KARADAĞ SARI
Keyword(s):  
Testosterone Level ◽  
Caspase 3 ◽  
Serum Testosterone ◽  
Testicular Tissue ◽  
Male Rats ◽  
Pde4 Inhibitor ◽  
Serum Testosterone Level ◽  
Sprague Dawley ◽  
Negative Effects ◽  
Testosterone Levels

Abstract Roflumilast (ROF) (3-cyclo-propylmethoxy-4-difuorome-thoxy-N-[3,5-di-chloropyrid-4-yl] benzamide) is a second generation and forcible phosphodiesterase-4 (PDE4) inhibitor. This study aims to investigate the effects of chronic Roflumilast in different doses on testicular tissue and testosterone levels in healthy Sprague-Dawley rats. During the research, the 6 weeks old (180–200 gr), 36 male rats were divided into 4 groups. Roflumilast (ROF) was administered as 0.5 mg/kg and 1 mg/kg by oral gavage for four weeks, once each day. Hematoxylin-Eosin (H&E) staining for histopathological examinations in testicular tissue, immunohistochemical and immunofluorescence examinations for Caspase-3, Apoptosis Inducing Factor (AIF) and Light Chain 3β (LC3B) expression levels, and ELISA method used to determine serum testosterone levels. Data were analyzed using SPSS v.22 with Kruskal-Wallis, Mann Whitney-U, and Wilcoxon tests. Roflumilast group lost weight compared to the control and sham. Shedding in the seminiferous epithelium, degenerations in the interstitial area, separation between cells, desquamation, interstitial edema and degenerative changes in the testicular tissue was observed. While apoptosis and autophagy determined by Caspase-3, AIF and LC3B were close and statistically insignificant in control and sham, there was significantly increased apoptotic and autophagic changes and immunopositivity in the ROF groups. The 1 mg/kg Roflumilast group’s serum testosterone level was lower than control, sham and 0.5 mg/kg Roflumilast groups. When the research data was evaluated, it was determined that the chronic use of the active ingredient Roflumilast, which has a broad-spectrum area such as COPD, arthritis, neurodegenerative diseases, liver and dermatology, had negative effects on the testicular tissue and testosterone level of rats.

scholarly journals Effect of tramadol dependence on male sexual dysfunction

2019 ◽  
Vol 12 (4) ◽  
pp. 157-162 ◽  
Author(s):  
Abdelmoty Kabbash ◽  
Rabab El Kelany ◽  
Merfat Oreby ◽  
Dina El Gameel
Keyword(s):  
Erectile Dysfunction ◽  
Prolactin Level ◽  
Premature Ejaculation ◽  
Positive Impact ◽  
Serum Testosterone ◽  
Serum Testosterone Level ◽  
Serum Prolactin ◽  
Control Group ◽  
Wide Availability ◽  
History Of

AbstractTramadol dependence became an increasing and alarming problem in the Egyptian community. Wide availability of tramadol as a pain killer and its role in the treatment of premature ejaculation may be the most apparent causes of increased magnitude of the problem among youth who believe that tramadol has a positive impact on their sexual functions. This study aimed to explore the real impact of chronic tramadol administration on sexual functions in males dependent on tramadol. The study was carried on 80 subjects (50 subjects were tramadol dependent group and 30 subjects represented the control group). Personal, family and past histories were obtained from all the participants in addition to the toxicological history from tramadol dependent group. Urine screening for tramadol was done for all cases of history of tramadol dependence then confirmation by HPLC technique to measure tramadol blood level was done. Both groups were investigated for serum testosterone and prolactin level. Curiosity (22%) and treatment of premature ejaculation (20%) were the main motives for dependence. Erectile dysfunction and decreased libido occurred in 44% and 48% of tramadol dependent group respectively. Significant increase in erectile dysfunction and decreased libido was noted as the duration of dependence increased. Additionally, significant decrease in serum testosterone level and increase in serum prolactin level as tramadol daily dose and duration increased was found. In conclusion, men who take tramadol for premature ejaculation or any other purpose must know that they are very susceptible to many sexual dysfunctions.

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