scholarly journals Effects of curcumin on sperm parameters abnormalities induced by morphine in rat

2017 ◽  
Vol 6 (2) ◽  
pp. 1-10
Author(s):  
S.H. Roshankhah ◽  
M.R. Salahshoor ◽  
S. Aryanfar ◽  
F. Jalili ◽  
M. Sohrabil ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Testosterone Level ◽  
Sperm Count ◽  
Serum Testosterone ◽  
Treated Group ◽  
Sperm Parameters ◽  
Male Rats ◽  
Serum Testosterone Level ◽  
Seminiferous Tubules ◽  
Control Group

Opioids are the most potent and effective analgesics available and have become accepted as appropriate treatment for acute, cancer and non-cancer. Morphine, which is commonly used for the treatment of severe pain, gastrointestinal tract and kidneys. Curcumin petals consist of, glycosides, flavonoids, and anthocyanin. The study aims at evaluating curcumin effect and morphine on sperm parameters, testis tissue and serum testosterone level in rat. In this experimental study, 48 male rats with 28 weeks of age and limited weight of 270 to 300g were selected. They were divided into eight groups of 6, untreated control group; morphine – treated group (20 mg/kg/day); curcumin -treated groups (10, 30, 60 mg/kg/day); and morphine and curcumin treated group intraperitoneal administration for successive 28 days. After 24hours animals were killed. Sperm motility was measured using WHO protocols. The sperm parameter such as motility, sperm count, morphology, seminiferous tubules diameter, weight testis, and serum testosterone level were analyzed (oneway ANOVA). Curcumin (10, 30 and 60 mg/kg) significantly increased mean percentage of sperm motility, count, testis weight, and serum testosterone level compared to control group (p<0.05). Testosterone level decreased significantly in rats treated with morphine. Co-administration of curcumin to morphine-treated rats improved the histopathological alterations induced by morphine in testis and increased the sperm count. Curcumin has a very strong antioxidant effects at applied doses and it can probably be used as an antioxidant and food supplement in reproductive disorders.Journal of Medical and Biomedical Sciences (2017) 6(2), 1-10

Vitamin C ameliorates the adverse effects of dexamethasone on sperm motility, testosterone level, and spermatogenesis indexes in mice

2018 ◽  
Vol 38 (4) ◽  
pp. 409-418 ◽  
Author(s):  
F Sadeghzadeh ◽  
MS Mehranjani ◽  
M Mahmoodi
Keyword(s):  
Adverse Effects ◽  
Vitamin C ◽  
Sperm Motility ◽  
Testosterone Level ◽  
Leydig Cells ◽  
Serum Testosterone ◽  
Serum Testosterone Level ◽  
Control Group ◽  
The Mean ◽  
Vit C

Background: Dexamethasone (DEX) is a common medicine that is capable of causing malformation in the male reproductive system. The aim of this study was to investigate the effect of vitamin C (Vit-C) on spermatogenesis indexes and daily sperm production (DSP) in adult mice treated with DEX. Methods: Male Naval Medical Research Institute (NMRI) mice were divided into four groups: Control, DEX (7 mg/kg/day), Vit-C (100 mg/kg/day), and DEX +Vit-C and treated for 7 days with intraperitoneal injection. Results: A significant increase in the mean levels of serum and tissue malondialdehyde (MDA) and apoptosis of Leydig cells was found in the DEX group compared to the control group. Sperm motility, DSP, tubular differentiation index, meiotic index, spermatogenesis index, the mean number of spermatocytes, round and long spermatids, and Leydig cells, and also serum testosterone level decreased in the DEX group compared to the control group. The results of this study indicate that Vit-C can significantly prevent the adverse effects of DEX on the mean number of spermatocyte, spermatid, and Leydig cells, tubular differentiation, meiotic and spermatogenesis index, DSP, sperm motility, and the mean levels of serum MDA. Conclusion: In conclusion, our results showed that coadministration of Vit-C and DEX prevents the adverse effects of DEX on the spermatogenesis indexes and DSP.

scholarly journals Estimate the level of testosterone hormone using I-chroma technique in males treated with Royal jelly and honeybee

2021 ◽  
Vol 4 (1) ◽  
pp. 16-18
Author(s):  
Baqer Hassan ◽  
Zainab Fadhil Abbas
Keyword(s):  
Testosterone Level ◽  
Royal Jelly ◽  
Serum Testosterone ◽  
Treated Group ◽  
Serum Testosterone Level ◽  
Control Group ◽  
Short Term Treatment ◽  
Testosterone Levels ◽  
Natural Treatment ◽  
Result Show

The experiment based on 10 matured men samples collected randomly from Baghdad Providence. The 10 samples divided into two groups, serum testosterone level of all 10 patient had been done using Ichroma technique. the treated group administered 2.5ml of honeybee with 350mg of royal jelly daily for (15day) while the second group stay as control group. After 15 days blood samples had been collected and serum separated to made another testosterone test for both groups using same i-chroma technique. The main Aim of this study is estimating the effect of royal jelly and honeybee as food on the testosterone hormone level in men. The result show that there was significant increase in the testosterone levels in treated group in comparison with control group. after experiment the result show significant increase in testosterone levels (in samples number 1,2,3,4 and 5) as the following (23.2 n.mo/L, 16.6 n.mo/L, 17.7 n.mo/L, 10.1 n.mo/L and 29.7 n.mo/L respectively. The study concluded that, short term treatment of royal jelly in combined with honey bee increasing the level of blood testosterone hormone in men in different ages significantly. The study either concluded that we can used honeybee and royal jelly as natural treatment of low testosterone level in men.

AMELIORATIVE EFFECTS OF MGS04 ON DEXAMETHASONE- INDUCED HISTO-CHEMICAL ALTERATIONS IN THE TESTES OF ALBINO RATS

1969 ◽  
Vol 6 (1) ◽  
pp. 753-757
Author(s):  
INAYATULLAH ◽  
MOHAMMAD KHAN ◽  
MARIYA HIDAYAT ◽  
LUTFUR-RAHMAN ◽  
ASMA SIDDIQUI ◽  
...  
Keyword(s):  
Testosterone Level ◽  
Serum Testosterone ◽  
Concomitant Administration ◽  
Testicular Tissue ◽  
Serum Testosterone Level ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Albino Rats ◽  
Male Adult ◽  
Group B

BACKGROUND: In experimental animal, dexamethasone-induced impaired spermatogenesis causesdisruption of the normal architecture of seminiferous tubules and alteration in male sexual hormonetestosterone. Concomitant administration of MgS04 preserved the cytoarchitecture of testes as well ashormonal regulation in albino rats.OBJECTIVE: This study was designed to observe the ameliorative effects of MgS04 on the histologyof testes and there correlation with serum testosterone level during dexamethasone administration inalbino rats.METHODOLOGY: This experimental study was conducted in the department of Anatomy, BasicA LMedical Sciences Institute (BMSI), Jinnah Postgraduate Medical Centre (JPMC), Karachi from 5 Aprilto 25th April 2012. Thirty healthy male adult albino rats were included in the study and divided equallyinto 3 groups. Each group comprising 10 animals. Group-A served as Control. Group-B receivedDexamethasone (intraperitonealy) at the dose of 4mg/kg body weight/24 hours. Group-C receivedDexamethasone at the same dose as in group-B and additionally given MgS04 (intramuscularly) at thedose of 20mg/kg/24hours.RESULTS: MgS04 significantly preserved the cytoarchitecture of testes, as well as minimizedalteration in serum testosterone level in group-C animals.CONCLUSION: MgSO4 has restored both the histological and biochemical damaging effects inducedby dexamethasone in Rats testes.KEYWORDS: Dexamethasone, MgS04, Testicular tissue, Testosterone.

scholarly journals EFFECTS OF AQUEOUS STEM BARK EXTRACT OF XYLOPIA AETHIOPICA ON THE REPRODUCTIVE FUNCTIONS OF MALE RATS

2018 ◽  
Vol 10 (1) ◽  
pp. 113
Author(s):  
Cyprien Magloire Hambe ◽  
Marie Ngaha Njila ◽  
Hubert Kenmogne ◽  
Brice Landry Koloko ◽  
Dieudonne Massoma Lembe
Keyword(s):  
Testosterone Level ◽  
Vas Deferens ◽  
Sperm Count ◽  
Serum Testosterone ◽  
Stem Bark ◽  
Male Rats ◽  
Serum Testosterone Level ◽  
Bark Extract ◽  
Stem Bark Extract ◽  
Xylopia Aethiopica

Objective: The sexual stimulatory effect of aqueous stem bark extract of Xylopia aethiopica which is commonly used traditionally for the treatment of fertility-related problems in males is reported in this work.Methods: The present study investigated the effect of 14 d oral administration of 1 ml/kg distilled water (control), 100, 200 and 300 mg/kg of body weight of the stem bark extract on some fertility parameters of the Swiss male rats. So, all the target organs (testis, epididymis, vas deferens, seminal vesicle and prostate) were weighed. The sperm motility and sperm count in testis, epididymis and vas deferens were determined. Serum and tissue protein, as well as cholesterol, were evaluated. The serum testosterone level was also assessed.Results: The extract did not produced significant change in the mobility of sperm, the sperm count in vas deferens, the relative weight of body and accessory organs at all doses. The daily sperm production (DSP) and the weight of prostate significantly increased at a dose of 100 mg/kg (p<0.01) while the sperm count in cauda epididymis increased at a dose of 200 mg/kg. The testicular and serum cholesterol significantly increased (p<0.001) at the dose of 100 mg/kg whereas the testicular and serum proteins increased (p<0.001) at the dose of 200 mg/kg. The serum testosterone level increased following 14 d of treatment (p<0.01) at the dose of 100 mg/kg.Conclusion: The results suggest that Xylopia aethiopica may have the potentials of being developed into a male fertility enhancing drug.

scholarly journals A study of the aphrodisiac properties of Cordyceps militaris in streptozotocin-induced diabetic male rats

2021 ◽  
Vol 14 (2) ◽  
pp. 537-544
Author(s):  
Toan Van Nguyen ◽  
Pramote Chumnanpuen ◽  
Kongphop Parunyakul ◽  
Krittika Srisuksai ◽  
Wirasak Fungfuang
Keyword(s):  
Testosterone Level ◽  
Serum Testosterone ◽  
Diabetic Rats ◽  
Cordyceps Militaris ◽  
The Body ◽  
Male Rats ◽  
Serum Testosterone Level ◽  
Control Group ◽  
Epididymal Sperm ◽  
Sperm Parameter

Background and Aim: Cordyceps militaris (CM) is a fungus that has been used to enhance aphrodisiac activity in men, but to date, no studies have focused on its antidiabetic properties. This study aimed to investigate the effects of CM on reproductive performance of streptozotocin (STZ)-induced diabetic male rats. Materials and Methods: Six-week-old Wistar rats were randomly divided into four groups: control Group 1 consisting of healthy rats; Group 2, healthy rats treated with CM (100 mg/kg); Group 3, diabetic untreated rats; and Group 4, diabetic rats treated with CM (100 mg/kg). Rats were orally administered with vehicle or CM for 21 days. The body weight, blood glucose level, food intake, epididymal sperm parameter, sexual behavior, serum testosterone level, and antioxidant parameters were determined. Results: The results indicated that CM treatment in STZ-induced diabetic rats significantly improved the epididymal sperm parameter and serum testosterone level and, in turn, their copulatory behavior. CM treatment in diabetic rats significantly ameliorated malondialdehyde level and significantly improved the glutathione and catalase levels. Conclusion: These results provide new information on the pharmacological properties of CM in ameliorating testicular damage due to oxidative stress and improving sexual performance in diabetic male rats.

Toxicological evaluation of hydroethanol leaf extract of Pupalia lappacea (Linn.) Juss. (Amaranthaceae) in rodents

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Murtala Akanji Abdullahi ◽  
Elijah Oladapo Oyinloye ◽  
Akinyinka Alabi ◽  
Aderonke Adeyinka Aderinola ◽  
Luqman Opeyemi Ogunjimi ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Density Lipoprotein ◽  
Serum Testosterone ◽  
Female Rats ◽  
Male Rats ◽  
Laboratory Animals ◽  
Serum Testosterone Level ◽  
Control Group ◽  
Toxicological Evaluation ◽  
Liver And Kidney

Abstract Objectives Several studies have established the ethnobotanical benefits of Pupalia lappacea (PL) in laboratory animals without extensive toxicological evaluation of its safety profiles. Thus, an extensive toxicological investigation of sub-chronic oral administration of the hydroethanol leaf extract of P. lappacea in rodents was carried out in this study. Methods Different groups of rats were treated orally with the extract (10, 50 and 250 mg/kg) daily for 90 consecutive days. The control group received distilled water (10 mL/kg). After 90 days, some rats were left for additional 30 days without treatment for reversibility study. Blood and organs samples were collected for different evaluations at the end of study periods. Results The extract decreased the bodyweights, feeding and water intakes in female rats. PL increased the weights of the liver and kidney in male rats. PL increased the red blood cell (RBC), packed cell volume (PCV), hemoglobin (Hb), triglycerides (TRIG), cholesterol and high density lipoprotein (HDL) contents in rats. PL (250 mg/kg) significantly reduced the sperm motility and serum testosterone level. Cyto-architectural distortions of the testes, liver and spleen were visible. Conclusions The findings showed that P. lappacea is relatively safe at lower doses but cautions should be taken at higher dose.

scholarly journals Effect of Tramadol on Sperm Profile of Male Albino Rats

2019 ◽  
pp. 1-6
Author(s):  
I. S. Esua ◽  
U. U. Uno ◽  
U. B. Ekaluo
Keyword(s):  
Sperm Motility ◽  
Sperm Count ◽  
Sperm Head ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Dependent Manner ◽  
Sperm Viability ◽  
Albino Rat ◽  
Significant Difference

Background and Aim: Tramadol is a potent analgesic effective in the treatment of mild to severe pains. However, the use of the drug can pose a threat to other organs and systems. Therefore, this study evaluated the effect of graded doses of tramadol on sperm profile of male albino rats. Materials and Methods: Eighteen male rats were divided into three groups (A, B and C) using completely randomized design (CRD) with six rats in each group. Rats in group A served as the control group and were given just food and water while groups B and C were given tramadol at 50 and 100 mg/kg body weight (BW) respectively, daily for the period of 65 days. The treatment was administered via oral gavage and at the end of the treatments, the rats were sacrificed. Immediately after sacrifice, a puncture was made in the epididymis with a sterile pin and examined for semen pH. The epididymes were processed for epididymal sperm motility, viability, count and sperm head abnormality. Results: There was no significant difference in the weight of testes and semen pH. Sperm viability, sperm motility, sperm count and weight of epididymes significantly reduced (p<0.05) in tramadol treated animals when compared with the control. Results also indicated statistically significant (p<0.05) increase in sperm head abnormalities in rats treated with tramadol when compared with the control. Conclusion: The results obtained from this study reveal that tramadol has negative effects on weight of epididymes, sperm count, sperm viability, sperm motility and sperm head abnormalities in male albino rat as mammalian models in a dose dependent manner.

New approach for reproductive toxicity assessment: chromatoid bodies as a target for methylmercury and polychlorinated biphenyls in prepubertal male rats

2020 ◽  
Vol 32 (10) ◽  
pp. 914
Author(s):  
M. S. Garcia ◽  
W. A. Orcini ◽  
R. L. Peruquetti ◽  
J. E. Perobelli
Keyword(s):  
Corn Oil ◽  
Morphological Changes ◽  
Synergistic Effects ◽  
Reproductive Toxicity ◽  
Treated Group ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Control Group ◽  
High Dose ◽  
Long Term Effects

This study investigated the reproductive toxicity of methylmercury (MeHg) and Aroclor (Sigma-Aldrich), alone or in combination, following exposure of prepubertal male rats considering the chromatoid body (CB) as a potential target. The CB is an important molecular regulator of mammalian spermatogenesis, primarily during spermatid cytodifferentiation. Male Wistar rats were exposed to MeHg and/or Aroclor , according the following experimental design: control group, which was administered in corn oil (vehicle) only; MeHg-treated group, which was administered 0.5mg kg−1 day−1 MeHg; Aroclor-treated group, which was administered 1mg kg−1 day−1 Aroclor; Mix-LD, group which was administered a low-dose mixture of MeHg (0.05mg kg−1 day−1) and Aroclor (0.1mg kg−1 day−1); and Mix-HD group, which was administered a high-dose mixture of MeHg (0.5mg kg−1 day−1) and Aroclor (1.0mg kg−1 day−1). MeHg was diluted in distilled water and Aroclor was made up in corn oil (volume 1mL kg−1). Rats were administered the different treatments from PND23 to PND53 by gavage, . The morphophysiology of CBs was analysed, together with aspects of steroid hormones status and regulation, just after the last treatment on PND53. In addition, the long-term effects on sperm parameters were assessed in adult animals. MeHg exposure increased mouse VASA homologue (MVH) protein levels in seminiferous tubules, possibly affecting the epigenetic status of germ cells. Aroclor produced morphological changes to CB assembly, which may explain the observed morphological defects to the sperm flagellum and the consequent decrease in sperm motility. There were no clear additive or synergistic effects between MeHg and Aroclor when administered in combination. In conclusion, this study demonstrates that MeHg and Aroclor have independent deleterious effects on the developing testis, causing molecular and morphological changes in CBs. To the best of our knowledge, this is the first study to show that CBs are targets for toxic agents.

scholarly journals The Effect of Eurycoma longifolia on Sperm Quality of Male Rats

2009 ◽  
Vol 4 (10) ◽  
pp. 1934578X0900401 ◽  
Author(s):  
Kit-Lam Chan ◽  
Bin-Seng Low ◽  
Chin-Hoe Teh ◽  
Prashanta K. Das
Keyword(s):  
Testosterone Level ◽  
Sperm Quality ◽  
Sperm Count ◽  
Male Rats ◽  
Plasma Testosterone ◽  
Seminiferous Tubules ◽  
Albino Rats ◽  
Meoh Extract ◽  
Plasma Testosterone Level ◽  
Etoac Fraction

The present study investigated the effects of a standardized methanol extract of E. longifolia Jack containing the major quassinoid constituents of 13α(21)-epoxyeurycomanone (1), eurycomanone (2), 13α,21-dihydroeurycomanone (3) and eurycomanol (4) on the epididymal spermatozoa profile of normal and Andrographis paniculata induced infertile rats. The standardized MeOH extract at doses of 50, 100 and 200 mg/kg, the EtOAc fraction (70 mg/kg), and standardized MeOH extract at 200 mg/kg co-administered with the EtOAc fraction of A. paniculata at 70 mg/kg were each given orally to male Sprague-Dawley albino rats for 48 consecutive days. The spermatozoa count, morphology, motility, plasma testosterone level and Leydig cell count of the animals were statistically analyzed by ANOVA with a post-hoc Tukey HSD test. The results showed that the sperm count of rats given the standardized MeOH extract alone at doses of 50, 100 and 200 mg/kg were increased by 78.9, 94.3 and 99.2 %, respectively when compared with that of control (p < 0.01). The low count, poor motility and abnormal morphology of the spermatozoa induced by the A. paniculata fraction were significantly reversed by the standardized MeOH extract of E. longifolia (p < 0.001). The plasma testosterone level of the rats treated with the standardized MeOH extract at 200 mg/kg was significantly increased (p < 0.01) when compared with that of the control and infertile animals. The spermatocytes in the seminiferous tubules and the Leydig cells appeared normal. Testosterone level was significantly higher in the testes (p < 0.01) than in the plasma after 30 days of oral treatment with the standardized MeOH extract. Interestingly, eurycomanone (2) alone was detected in the rat testis homogenates by HPLC-UV and confirmed by LC/MS, and may have contributed towards the improvement of sperm quality. Thus, the plant may potentially be suitable for the management of male infertility.

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