The Morphological Analysis of Cells in the Bronchoscopic Brushing and TBNA of Patients with Lung Adenocarcinoma

Biopsy, brushing, and transbronchial needle aspiration (TBNA) are the most common methods for diagnosis of lung adenocarcinoma and are taken during the same diagnostic bronchoscopic procedure. However, it is not clear what the morphological diagnostic criteria of cytology by brushing or TBNA are. A retrospective analysis was performed on 136 patients who underwent video bronchoscopy examination for diagnostic purposes. All the subjects were performed brushing or TBNA and confirmed as lung adenocarcinoma by biopsy or postoperative pathology. An additional 140 randomly selected patients with benign lung diseases were included in the study and used as a control group. The benign cells usually confused with adenocarcinoma cells were ciliated columnar cells, mucous columnar cells, ciliated cuboid cells, and reactive ciliated cells, respectively. The number of cases diagnosed as adenocarcinoma cells, carcinoma cells, suspicious cancer cells, and atypical proliferative cells by cytology was 101, 11, 20, and 4, respectively. The main basis for the interpretation of adenocarcinoma cells is the enlargement of individual nucleus, the arrangements of multistage papillary, and the general enlargement of nuclei, while the main clue for the interpretation of suspicious cancer cells and dysplasia cells comes from escape cells. The results suggested that the degree of nuclear enlargement, multiple papillary arrangement, and escape cells or escape trend cells are important clues for the interpretation of lung adenocarcinoma cells, while the atypical proliferative cells were similar to escape cells or escape trend cells, which were essentially benign cells beside the cancer.

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Sarcoid-like reaction associated with lung adenocarcinoma: A case report

Proceedings of Singapore Healthcare ◽

10.1177/2010105818766363 ◽

2018 ◽

Vol 28 (1) ◽

pp. 68-70

Author(s):

Qiao Li Tan ◽

Leong Chai Leow ◽

Salahudeen Mohamed Haja Mohideen ◽

Duu Wen Sewa

Keyword(s):

Lung Adenocarcinoma ◽

Lower Lobe ◽

Rare Condition ◽

Disease Recurrence ◽

Needle Aspiration ◽

Endobronchial Ultrasound ◽

Transbronchial Needle Aspiration ◽

Surveillance Imaging ◽

Bilateral Hilar Lymphadenopathy ◽

History Of

A 71-year-old woman with a history of left lower lobe lobectomy 4 years ago for adenocarcinoma of the lung presented with new mediastinal and bilateral hilar lymphadenopathy on surveillance imaging, suspicious for disease recurrence. She underwent an endobronchial ultrasound-guided transbronchial needle aspiration of the lymph nodes and histology yielded non-necrotizing epithelioid granulomas with no evidence of malignancy. A diagnosis of sarcoid-like reaction associated with lung adenocarcinoma was made. The significance of this rare condition is discussed.

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Conventional transbronchial needle aspiration is promising for identifying EGFR mutations in lung adenocarcinoma

Thoracic Cancer ◽

10.1111/1759-7714.13014 ◽

2019 ◽

Vol 10 (4) ◽

pp. 856-863

Author(s):

Li‐Han Hsu ◽

Jen‐Sheng Ko ◽

Chia‐Chuan Liu ◽

An‐Chen Feng ◽

Nei‐Min Chu

Keyword(s):

Lung Adenocarcinoma ◽

Needle Aspiration ◽

Egfr Mutations ◽

Transbronchial Needle Aspiration

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miR-9 Does Not Regulate Lamin A Expression in Metastatic Cells from Lung Adenocarcinoma

International Journal of Molecular Sciences ◽

10.3390/ijms21051599 ◽

2020 ◽

Vol 21 (5) ◽

pp. 1599

Author(s):

Julien Guinde ◽

Audrey Benoit ◽

Diane Frankel ◽

Stéphane Robert ◽

Kevin Ostacolo ◽

...

Keyword(s):

Lung Adenocarcinoma ◽

Cell Sorting ◽

Epithelial Membrane Antigen ◽

Carcinoma Cells ◽

Lamin A ◽

Neural Cells ◽

Metastatic Cells ◽

Adenocarcinoma Cells ◽

Metastatic Process ◽

Central Actor

In lung adenocarcinoma, low lamin A expression in pleural metastatic cells has been proposed as a pejorative factor. miR-9 physiologically inhibits the expression of lamin A in neural cells and seems to be a central actor in the carcinogenesis and the metastatic process in lung cancer. Thus, it could be a good candidate to explain the reduction of lamin A expression in lung adenocarcinoma cells. miR-9 expression was analyzed in 16 pleural effusions containing metastatic cells from lung adenocarcinoma and was significantly reduced in patients from the ‘Low lamin A expression’ group compared to patients from the ‘High lamin A expression’ group. Then, carcinoma cells selection by fluorescence-activated cell sorting (FACS) was performed according to epithelial membrane antigen (EMA) expression, reflecting lamin A expression. miR-9 was underexpressed in lamin A− carcinoma cells compared to lamin A+ carcinoma cells in patients from the ‘Low lamin A expression’ group, whereas there was no difference of miR-9 expression between lamin A+ and lamin A− carcinoma cells in patients from the ‘High lamin A expression’ group. These results suggest that miR-9 does not regulate lamin A expression in metastatic cells from lung adenocarcinoma. On the contrary, miR-9 expression was shown to be reduced in lamin A-negative carcinoma cells.

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ITRAQ-Based Proteomics Analysis of Triptolide On Human A549 Lung Adenocarcinoma Cells

Cellular Physiology and Biochemistry ◽

10.1159/000487286 ◽

2018 ◽

Vol 45 (3) ◽

pp. 917-934 ◽

Cited By ~ 11

Author(s):

Fangqiong Li ◽

Dongxiao Zhao ◽

Suwen Yang ◽

Juan Wang ◽

Qin Liu ◽

...

Keyword(s):

Lung Cancer ◽

Lung Adenocarcinoma ◽

Anticancer Activity ◽

Cancer Cells ◽

Cancer Cell ◽

Cell Apoptosis ◽

A549 Cells ◽

Adenocarcinoma Cells ◽

Lung Adenocarcinoma Cells ◽

A549 Lung

Background/Aims: Triptolide (TP) is a diterpenoid triepoxide extracted from the traditional Chinese medical herb Tripterygium wilfordii that exerts prominent broad-spectrum anticancer activity to repress proliferation and induce cancer cell apoptosis through various molecular pathways. We previously observed that TP inhibits the progression of A549 cells and pancreatic cancer cells (PNCA-1) in vitro. However, the complex molecular mechanism underlying the anticancer activity of TP is not well understood. Methods: To explore the molecular mechanisms by which TP induces lung cancer cell apoptosis, we investigated changes in the protein profile of A549 cells treated with TP using a proteomics approach (iTRAQ [isobaric tags for relative and absolute quantitation] combined with NanoLC-MS/MS [nano liquid chromatography-mass spectrometry]). Changes in the profiles of the expressed proteins were analyzed using the bioinformatics tools OmicsBean and the Kyoto Encyclopedia of Genes and Genomes (KEGG) and were verified using western blotting. Apoptosis and cell cycle effects were analyzed using flow cytometry. Results: TP induced apoptosis in A549 cells and blocked A549 cells at the G2/M phase. Using iTRAQ technology, we observed 312 differentially expressed proteins associated in networks and implicated in different KEGG pathways. Gene Ontology (GO) analysis showed the overviews of dysregulated proteins in the biological process (BP), cell component (CC), and molecular function (MF) categories. Moreover, some candidate proteins involved in PARP1/AIF and nuclear Akt signaling pathways or metastasis processes were validated by western blotting. Conclusion: TP exerted anti-tumor activity on non-small cell lung cancer (NSCLC) A549 lung adenocarcinoma cells by dysregulating tumor-related protein expression. Herein, we provide a preliminary study of TP-related cytotoxicity on A549 cells using proteomics tools. These findings may improve the current understanding of the anti-tumor effects of TP on lung cancer cells and may reveal candidate proteins as potential targets for the treatment of lung cancer.

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Momordica charantiaExtract Induces Apoptosis in Human Cancer Cells through Caspase- and Mitochondria-Dependent Pathways

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2012/261971 ◽

2012 ◽

Vol 2012 ◽

pp. 1-11 ◽

Cited By ~ 19

Author(s):

Chia-Jung Li ◽

Shih-Fang Tsang ◽

Chun-Hao Tsai ◽

Hsin-Yi Tsai ◽

Jong-Ho Chyuan ◽

...

Keyword(s):

Cell Death ◽

Cytotoxic Activity ◽

Cancer Cells ◽

Dna Fragmentation ◽

Human Cancer ◽

Momordica Charantia ◽

Carcinoma Cells ◽

Human Cancer Cells ◽

Adenocarcinoma Cells ◽

Anti Cancer

Plants are an invaluable source of potential new anti-cancer drugs.Momordica charantiais one of these plants with both edible and medical value and reported to exhibit anticancer activity. To explore the potential effectiveness ofMomordica charantia, methanol extract ofMomordica charantia(MCME) was used to evaluate the cytotoxic activity on four human cancer cell lines, Hone-1 nasopharyngeal carcinoma cells, AGS gastric adenocarcinoma cells, HCT-116 colorectal carcinoma cells, and CL1-0 lung adenocarcinoma cells, in this study. MCME showed cytotoxic activity towards all cancer cells tested, with the approximate IC50ranging from 0.25 to 0.35 mg/mL at 24 h. MCME induced cell death was found to be time-dependent in these cells. Apoptosis was demonstrated by DAPI staining and DNA fragmentation analysis using agarose gel electrophoresis. MCME activated caspase-3 and enhanced the cleavage of downstream DFF45 and PARP, subsequently leading to DNA fragmentation and nuclear condensation. The apoptogenic protein, Bax, was increased, whereas Bcl-2 was decreased after treating for 24 h in all cancer cells, indicating the involvement of mitochondrial pathway in MCME-induced cell death. These findings indicate that MCME has cytotoxic effects on human cancer cells and exhibits promising anti-cancer activity by triggering apoptosis through the regulation of caspases and mitochondria.

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An Investigation on Cytotoxicity Effect of Putrescine-Sulphur Analogues on Breast Cancer (Mcf-7), Human Lung Adenocarcinoma (A549) and Colorectal Cancer (Hct-8) Cell Lines

IIUM Medical Journal Malaysia ◽

10.31436/imjm.v16i1.1135 ◽

2017 ◽

Vol 16 (1) ◽

Author(s):

Norsuhana Halim ◽

Radiah Abdul Ghani ◽

Adzly Hairee Sahabudin ◽

Fiona How Ni Fong

Keyword(s):

Lung Adenocarcinoma ◽

Cancer Cells ◽

Cell Lines ◽

Human Lung ◽

Breast Adenocarcinoma ◽

Normal Cells ◽

Human Lung Adenocarcinoma ◽

Adenocarcinoma Cells ◽

Specific Cancer ◽

Mcf 7

Introduction: Cancer is one of the global health problems that has a detrimental effect to a person's life. However, chemotherapeutic agents success are subject to the side effects due to lack of specificity in the drug delivery system to cancer cells and an increase risk of systemic toxicity to the normal cells. Polyamine transport system (PTS) is one of the potential pathways for transporting anticancer agent into specific cancer cells. This is due to the upregulation of PTS in cancer cells compared to normal cells for the proliferation activity. The aim of this study was to investigate the cytotoxicity effect of putrescine-sulphur analogues type 1 (PSA-1) and type 2 (PSA-2) on human lung adenocarcinoma cells (A549), human colorectal adenocarcinoma cells (HCT-8) and human breast adenocarcinoma cell (MCF-7). Materials and method: The cytotoxicity effect of newly synthesized PSA-1 and PSA-2 were evaluated on selected cancer cells; MCF-7, A549 and HCT-8 cell lines. The halfmaximal inhibitory concentration (IC50) obtained from tetrazolium bromide (MTT) assay was derived from the dose-response graph for all cell lines. Results: PSA-2 elicited cytotoxicity effect, eventhough the IC50 values were not potent with IC50 of 5.4 mM, 5.2 mM and 7.0 mM for MCF-7/48h, A549/48h and HCT-8/48h, respectively. The PSA-1 compound exhibited cytotoxicity effect in all cell lines, however, the compound failed to induce anti-proliferation at the concentration of 3 mM and above. The cytotoxicity of PSA-2 compound against MCF-7 cell lines showed higher potency compared to A549 and HCT-8 cell lines. Conclusion: It was suggested that PSA-2 compound was able to exert cytotoxicity effect against selected cancer cells, in low potency and is deemed for further investigation to increase its effectiveness against specific cancer cells.

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Transbronchial needle aspiration for lung cancer patients, especially for the cases in which cancer cells were negative in the specimens taken by the brushing method

The Journal of the Japanese Society of Clinical Cytology ◽

10.5795/jjscc.16.182 ◽

1977 ◽

Vol 16 (2) ◽

pp. 182-187

Author(s):

Hirotoshi SATO ◽

Eiichi AKAOGI ◽

Yasuki SAITO ◽

Takashi KONDO ◽

Hideichi SUDA ◽

...

Keyword(s):

Lung Cancer ◽

Cancer Cells ◽

Cancer Patients ◽

Needle Aspiration ◽

Transbronchial Needle Aspiration ◽

Lung Cancer Patients

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Mediastinal lymph node staging by EBUS-TBNA and CT: Characteristics of lymph node enlargement caused by anthracosis and other benign conditions

International Journal of Diagnostic Imaging ◽

10.5430/ijdi.v1n2p64 ◽

2014 ◽

Vol 1 (2) ◽

pp. 64

Author(s):

Johannes Kirchner ◽

Michael Broll ◽

Philipp Müller ◽

Esther Maria Kirchner ◽

Natalia Pomjanski ◽

...

Keyword(s):

Lymph Node ◽

Lymph Nodes ◽

Mediastinal Lymph Node ◽

Needle Aspiration ◽

Endobronchial Ultrasound ◽

Control Group ◽

Lymph Node Staging ◽

Ultrasound Guided ◽

Transbronchial Needle Aspiration ◽

Lymph Node Enlargement

Objectives: Aim of this comparative study was to assess the accuracy of computed tomography (CT) and endobronchial ultrasound guided transbronchial needle aspiration (EBUS-TBNA) for mediastinal lymph node staging in cases of lymph node enlargement due to anthracosis and other benign conditions. Methods: In a retrospective analysis we report on the MSCT findings of 39 patients (28 males, 11 females) with EBUS-TBNA confirmed diagnosis of 53 enlarged lymph nodes due to anthracosis. A control group comprised 20 consecutive patients with 27 enlarged lymph nodes (11 males, 9 females) due to chronic lymphadenopathy (n = 14) or sarcoidosis (n = 13). Results: No significant differences were observed between the two groups regarding size (mean short axis diameter 13.7mm vs. 14.5mm), shape (most often oval) or presence of lymph node confluence (32.1% vs. 33.3%), contrast enhancement (3.8% vs. 3.7%), and fatty involution (3.8% vs. 3.7%). In comparison with the control group anthracotic lymph nodes were significantly less often ill-defined in EBUS (5.7 vs. 25.9, p = 0.025) as well as in CT (1.9% vs. 18.5%, p = 0.01), but more often showed calcifications in CT (24.5% vs. 3.7%, p = 0.017). Lymph node colliquation was seen neither in anthracosis nor in other benign conditions. Conclusions: Mediastinal lymph node enlargement due to anthracosis, lymphadenopathy and sarcoidosis show some different findings in EBUS and CT but cannot definitely be differentiated. Advances in knowledge: Radiologists should be aware of mediastinal lymph node enlargement due to anthracosis.

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