scholarly journals Comparison of cross-matching method for detection of DEA 7 blood incompatibility

2018 ◽  
Vol 30 (6) ◽  
pp. 911-916 ◽  
Author(s):  
Eva Spada ◽  
Roberta Perego ◽  
Luis Miguel Viñals Flórez ◽  
Maria del Rosario Perlado Chamizo ◽  
Luciana Baggiani ◽  
...  
Keyword(s):  
Reference Method ◽  
Kappa Coefficient ◽  
Plasma Samples ◽  
Matching Method ◽  
Double Blind ◽  
Perfect Agreement ◽  
Erythrocyte Antigen ◽  
Cross Matching ◽  
Cross Match ◽  
Dog Erythrocyte

We compared 3 major cross-match (XM) tests to identify dog erythrocyte antigen (DEA) 7 blood incompatibilities in dogs as a result of anti–DEA 7 antibodies: gel (GEL), standard tube (TUBE) agglutination, and immunochromatography strips (STRIP). Blood samples from 42 dogs were typed for DEA 7; 2 tested DEA 7–positive (DEA 7+). The 40 DEA 7–negative (DEA 7–) plasma samples were cross-matched against the 2 DEA 7+ and 3 DEA 7– red blood cell (RBC) samples by GEL to identify samples with anti–DEA 7 antibodies. Twenty DEA 7– plasma samples without and with anti–DEA 7 antibodies were cross-matched with samples of the 2 DEA 7+ RBCs in a double-blind fashion using the TUBE and STRIP XM methods. GEL results were used as the reference method for comparison. To determine relationships between results, 2 × 2 tables were used. Cohen kappa coefficient (κ) was calculated between results of GEL and the other 2 methods. With GEL, 21 of 40 XM tests were positive and 19 of 40 negative for anti–DEA 7 antibodies. The same results were obtained by TUBE, whereas only 1 of 40 XM tests was positive by STRIP. There was a statistically significant relationship between results of GEL and TUBE ( p < 0.000) with perfect agreement (κ = 1.000), but not between GEL and STRIP results ( p = 1.000) in which agreement was equivalent to chance (κ = 0.0453). The GEL and TUBE XM tests, but not STRIP, are useful methods for identification of DEA 7 incompatibilities caused by anti–DEA 7 antibodies.

scholarly journals Gaia Data Release 2

2019 ◽  
Vol 621 ◽  
pp. A144 ◽  
Author(s):  
P. M. Marrese ◽  
S. Marinoni ◽  
M. Fabrizio ◽  
G. Altavilla
Keyword(s):  
Global Analysis ◽  
Data Retrieval ◽  
High Accuracy ◽  
Data Archives ◽  
Data Release ◽  
Astronomical Research ◽  
The Cross ◽  
Single Data ◽  
Cross Matching ◽  
Cross Match

Context. Although the Gaia catalogue on its own is a very powerful tool, it is the combination of this high-accuracy archive with other archives that will truly open up amazing possibilities for astronomical research. The advanced interoperation of archives is based on cross-matching, leaving the user with the feeling of working with one single data archive. The data retrieval should work not only across data archives but also across wavelength domains. The first step for a seamless access to the data is the computation of the cross-match between Gaia and external surveys. Aims. We describe the adopted algorithms and results of the pre-computed cross-match of the Gaia Data Release 2 (DR2) catalogue with dense surveys (Pan-STARRS1 DR1, 2MASS, SDSS DR9, GSC 2.3, URAT-1, allWISE, PPMXL, and APASS DR9) and sparse catalogues (HIPPARCOS2, Tycho-2, and RAVE 5). Methods. A new algorithm is developed specifically for sparse catalogues. Improvements and changes with respect to the algorithm adopted for DR1 are described in detail. Results. The outputs of the cross-match are part of the official Gaia DR2 catalogue. The global analysis of the cross-match results is also presented.

Do We Need to Cross Match Blood for Elective Laminectomy?

Neurosurgery ◽  
1983 ◽  
Vol 13 (5) ◽  
pp. 569-571
Author(s):  
Deba P. Sarma
Keyword(s):  
General Hospital ◽  
Personal Experience ◽  
Blood Bank ◽  
Antibody Screen ◽  
Cross Matching ◽  
Cross Match ◽  
Blood Data

Abstract Elective laminectomy cases do not need a routine order for typing and cross matching of blood. Data from the literature and from personal experience in the blood bank of a 580-bed general hospital are presented to support this conclusion. An ABO-Rh type and an antibody screen can safely substitute for routine cross matches for such cases. The use of type and screen rather than cross match allows the blood bank to distribute limited blood resources more efficiently without jeopardizing the patients.

Repeatability indices for the Farnsworth D-15 test

2004 ◽  
Vol 21 (3) ◽  
pp. 449-453 ◽  
Author(s):  
JEFFERY K. HOVIS ◽  
SHANKARAN RAMASWAMY ◽  
MATTHEW ANDERSON
Keyword(s):  
Color Vision ◽  
Kappa Coefficient ◽  
Clinical Population ◽  
Perfect Agreement ◽  
Green Color ◽  
Color Vision Defect ◽  
Vision Defect ◽  
Subject Pool ◽  
Color Vision Test ◽  
Test Result

The repeatability of the D-15 color-vision test is considered to be excellent. However, this conclusion is based on a subject pool which contained a large percentage of color-normals. This type of sampling could bias the repeatability results because color-normals rarely fail the test. Furthermore, color-normals usually do not perform the D-15 in the clinical setting. To establish the repeatability of the D-15 for a relevant clinical population, we examined the D-15 results from two different sessions for 116 subjects who had a congenital red–green color-vision defect. The kappa coefficient for intersession agreement indicated that approximately 84% of the subjects obtained the same pass/fail results at both sessions. The type of defect was repeatable on approximately 80% of the subjects. Although the repeatability of the D-15 for color-defective subjects was good, it was lower than the near-perfect agreement reported previously. The coefficients of repeatability for the crossings show that if a person makes less than five crossings then the test should be administered again in order to ensure that the test result is repeatable.

Evaluation of soluble KIT (sKIT) as a potential surrogate marker for TTP in sunitinib malate (SU)-treated patients (pts) with advanced GIST

2007 ◽  
Vol 25 (18_suppl) ◽  
pp. 10007-10007 ◽  
Author(s):  
M. Blackstein ◽  
X. Huang ◽  
G. D. Demetri ◽  
P. G. Casali ◽  
C. R. Garrett ◽  
...  
Keyword(s):  
Kinase Inhibitor ◽  
Surrogate Marker ◽  
Sampling Period ◽  
Clinical Results ◽  
Phase Iii ◽  
Plasma Samples ◽  
Double Blind ◽  
Cox Models ◽  
Time Changes ◽  
The Impact

10007 Background: SU is an oral, multitargeted tyrosine kinase inhibitor of KIT, PDGFRs, VEGFRs, RET and FLT3, approved multinationally for the treatment of imatinib (IM)-resistant/-intolerant GIST. Preliminary analysis in a SU phase I/II GIST study suggested that a decline in plasma sKIT levels may correlate with measures of clinical benefit. We evaluated the potential of sKIT as a surrogate marker for TTP using samples obtained in a randomized, double-blind, placebo-controlled phase III study of SU in pts with IM-resistant/-intolerant GIST, clinical results of which have been reported previously. Methods: 312 pts were randomized (2:1) to receive SU 50 mg (n=207) or placebo (n=105) daily in 6-wk cycles (4 wks on treatment, 2 wks off), respectively. The primary endpoint was TTP as per RECIST. Levels of sKIT in plasma samples were measured in cycle 1 on days 1, 14 and 28 and in cycles 2 and 3 on days 1 and 28 using a performance-validated ELISA. Prentice Criterion, Cox models and the Proportion of Treatment Effect (PTE) were used to analyze the results (PTE of 1 = ideal surrogate). Results: Numbers of pts with matched pairs of baseline and on-study plasma samples varied from 228 pts at cycle 1, day 14 to 106 pts at the end of cycle 3. After 4 wks of treatment (cycle 1, day 28), plasma sKIT levels began to exhibit a significant decrease (P<0.0001) in response to SU treatment; at the same time, changes in the level of sKIT (decreases vs. increases) also became indicators of TTP (HR=0.53; 95% CI, 0.37–0.75; P=0.0003), with decreases associated with longer TTP. This trend continued throughout the sampling period, with the effect persisting off treatment. Although the impact of SU on plasma sKIT levels continued to be seen after 2 cycles, sKIT changes became a better indicator of TTP than initial treatment group by cycle 2, day 28 (PTE = 0.62; HR=0.51; 95% CI, 0.38–0.69; P<0.0001), and at cycle 3, day 1 (PTE = 0.64; HR=0.42; 95% CI, 0.29–0.60; P<0.0001). Conclusion: These preliminary findings suggest that circulating sKIT may be a surrogate marker for TTP in GIST pts after 2 cycles of SU treatment. Further studies are warranted to confirm these findings and to establish whether sKIT can be used as a general surrogate marker of clinical outcomes in GIST pts with SU or other therapies. [Table: see text]

Flow cytometric assessment of canine erythrocytes and platelets for dog erythrocyte antigen 1.1

2011 ◽  
Vol 40 (4) ◽  
pp. 435-443 ◽  
Author(s):  
Cynthia de A. Lucidi ◽  
Regina K. Takahira ◽  
John A. Gerlach ◽  
John M. Davis ◽  
Kenneth A. Schwartz ◽  
...  
Keyword(s):  
Flow Cytometric ◽  
Erythrocyte Antigen ◽  
Dog Erythrocyte

scholarly journals Preliminary Investigation of Bovine Whole Blood Xenotransfusion as a Therapeutic Modality for the Treatment of Anemia in Goats

2021 ◽  
Vol 8 ◽  
Author(s):  
Joe S. Smith ◽  
Austin K. Viall ◽  
Ryan M. Breuer ◽  
Rebecca A. Walton ◽  
Paul J. Plummer ◽  
...  
Keyword(s):  
Whole Blood ◽  
Blood Donors ◽  
Preliminary Investigation ◽  
Therapeutic Modality ◽  
Appropriate Treatment ◽  
Common Clinical Presentation ◽  
Cattle Blood ◽  
Future Work ◽  
Cross Matching ◽  
Cross Match

Anemia requiring whole blood transfusion for appropriate treatment is a common clinical presentation of caprine patients to veterinary practitioners; however, identifying suitable blood donors in goat herds can be challenging. In other veterinary species, the practice of xenotransfusion, where blood from 1 species is transfused to another, is used in emergency settings. Due to their ability to donate large volumes of whole blood, cattle could be an ideal source for xenotransfusion of goats. In this study 2 healthy goats were transfused with bovine whole blood. The goats were then monitored for adverse effects and the presence of bovine erythrocyte post-xenotransfusion. Afterward, 15 caprine–bovine combinations were evaluated for compatibility via cross-matching. Both goats tolerated xenotransfusion, although transient reactions were observed. Of the 15 cross-match combinations, 11 of the major cross matches were compatible, and all minor cross matches were also compatible. While future work is necessary to refine this technique, xenotransfusion of goats with cattle blood may be a therapeutic modality for the treatment of caprine anemia.

Effect of Isotretinoin on Endogenous Tissue-Type Plasminogen Activator (t-PA) and Plasminogen Activator Inhibitor 1 (PAI-1) in Humans

1993 ◽  
Vol 70 (06) ◽  
pp. 1005-1008 ◽  
Author(s):  
A E Wallnöfer ◽  
J M T van Griensven ◽  
H C Schoemaker ◽  
A F Cohen ◽  
W Lambert ◽  
...  
Keyword(s):  
Plasminogen Activator ◽  
Circadian Variation ◽  
Plasminogen Activator Inhibitor ◽  
Placebo Treatment ◽  
Significant Rise ◽  
Tissue Type ◽  
Plasma Samples ◽  
Plasminogen Activator Inhibitor 1 ◽  
Double Blind ◽  
Pai 1

SummaryThe effect of isotretinoin on fibrinolysis was investigated in 10 healthy, male volunteers in a randomized, double-blind, crossover-designed study. Isotretinoin (40 mg) was administered in the morning and in the evening for 5 days. t-PA, u-PA and PAI-1 antigen and activity in plasma were measured every morning at 9 a.m. on days 1 to 4 and every 3 hours over 24 hours on day 5. Isotretinoin treatment had no significant stimulatory effect on endogenous t-PA antigen and activity in morning plasma samples nor on their circadian variation. Also, u-PA antigen levels did not change after isotretinoin treatment. Mean PAI-1 antigen and PAI activity in 9 a.m. plasma samples were non-significantly higher during isotretinoin than during placebo treatment. After treatment with isotretinoin a significant rise of fasting triglyceride plasma levels was observed as compared to placebo. The study shows that isotretinoin has no clinically significant effect on endogenous fibrinolysis.

scholarly journals Alloimmunization of a dog erythrocyte antigen 1− dog transfused with weakly dog erythrocyte antigen 1+ blood

2019 ◽  
Vol 33 (5) ◽  
pp. 2037-2045 ◽  
Author(s):  
Maryline Guidetti ◽  
Isabelle Goy‐Thollot ◽  
Catherine Boisvineau ◽  
Urs Giger
Keyword(s):  
Erythrocyte Antigen ◽  
Dog Erythrocyte

An Evaluation of Currently Available Methods for Plasma Fibrinogen

1979 ◽  
Author(s):  
K.A. Rickard ◽  
J. Burridge ◽  
T. Exner ◽  
P. Power
Keyword(s):  
Ammonium Sulphate ◽  
Fibrinogen Level ◽  
Reference Method ◽  
Test Methods ◽  
Sodium Sulphite ◽  
Plasma Fibrinogen ◽  
Alkaline Solutions ◽  
Fibrinogen Concentration ◽  
Thrombin Time ◽  
Plasma Samples

An assessment of fibrinogen concentration is basic to any investigation of clotting dysfunction and often an estimate of fibrinogen level is needed rapidly as an indication of consumptive coagulopathy or fibrinolysis. Fibrinogen levels in a variety of clinical plasma samples were assessed concurrently by several methods. Results were correlated against a reference method based on Ancrod-clottable fibrinogen and calibrated by U.V. absorbance with alkaline solutions of carefully dried fibrin standard. The best correlations with the reference method were achieved by an immunologic method using the Centrifichem principle and by heat precipitation with quantitation by packing in micro-haematocrit tubes. A modified clot opacity method also gave acceptable results. The turbidimetric ammonium sulphate and sodium sulphite precipitation methods correlated less well with the reference method, and in particular the sodium sulphite technique gave high apparent fibrinogen levels with jaundiced plasmas. Neither of the turbidimeteric methods were useful for fibrinogen levels below 50mg/dl. The thrombin time method showed excellent sensitivity to fibrinogen, even at very low fibrinogen levels, but did not correlate well with the reference method. This apparently conflicts with the findings of a recent CAP survey which strongly favoured the thrombin time method. We believe there is a danger that such surveys promote test methods on which there is good inter-labotatory agreement, but which may not be specific in function.

Sign in / Sign up

Export Citation Format

Share Document