Bioavailability and radical scavenging power of phenolic compounds of cocoa and coffee mixtures

2021 ◽  
pp. 108201322110232
Author(s):  
Elly Acosta-Otálvaro ◽  
Raúl Domínguez-Perles ◽  
Juan Camilo Mazo-Rivas ◽  
Cristina García-Viguera
Keyword(s):  
Phenolic Compounds ◽  
Epithelial Transport ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Intestinal Barrier ◽  
In Vitro Models ◽  
Absorption Efficiency ◽  
Set Up

Cocoa and coffee are natural sources of phenolic compounds, which are degraded during beans processing of both. For this reason, there is an interest in obtaining extracts of these bio compounds. The aim of this study was uncovering the radical scavenging activity (AC) of mixtures of cocoa and coffee extracts, and the bioavailability of their phenolic compounds, resorting to in vitro models: DPPH and ORAC antioxidant methods, and the characterization of the trans-epithelial transport of cocoa and coffee phenolics through Caco-2 cells monolayer model of the intestinal barrier. The cocoa displayed a higher AC than the coffee regarding both DPPH and ORAC assays, while the mixtures increased in parallel with the percentage of cocoa in the blends. The combination index was calculated to set up the type of interaction in the cocoa-coffee mixtures, obtaining that the mixture 25:75 was moderately antagonistic, 50:50 nearly additive, and 75:25 slightly synergistic. The absorption efficiency of the cocoa phenolic compounds was between 87.9%–97.4%, in the coffee compounds was 100%. The mixtures varied according to the proportion of cocoa and coffee. The results obtained allowed concluding that the phenolic compounds present in cocoa and coffee, respectively, are featured by high bioavailability and a valuable antioxidant capacity, while no pattern was found in the mixtures concerning the real benefit of using them combined.

scholarly journals Comparative Evaluation of Argania spinosa and Olea europaea Leaf Phenolic Compounds and their Antioxidant Activity

Botanica ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 76-87
Author(s):  
Aziza Lfitat ◽  
Hind Zejli ◽  
Abdelkamel Bousselham ◽  
Yassine El Atki ◽  
Badiaa Lyoussi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Olive Leaves ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Total Flavonoids Content

AbstractWe conducted this study to determine and compare the content of phenolic compounds and flavonoids in the argan and olive leaves as well as their antioxidant capacity in aqueous, methanolic, and ethyl acetate extracted fractions. In vitro antioxidant activity was evaluated in comparison with synthetic antioxidants by assessing DPPH• radical scavenging capacity, ferric reducing antioxidant power, scavenging ability by inhibiting the β-carotene/linoleic acid emulsion oxidation, and by the ABTS radical scavenging activity assay. Total phenolic content in argan samples ranged from 221.69 ± 2.07 to 1.32 ± 0.01 mg GAE/g DW and in olive samples from 144.61 ± 0.82 to 1.21 ± 0.02 mg GAE/g DW. Total flavonoids content in argan samples varied from 267.37 ± 1.12 to 25.48 ± 0.02 mg QE/g DW, while in olives from 96.06 ± 0.78 to 10.63 ± 0.05 mg QE/g DW. In vitro antioxidant studies strongly confirmed the antioxidant potency of argan and olive leaves and their richness in secondary metabolites that are effective in free radicals scavenging and metal chelating capacities, indicating their antioxidant power.

scholarly journals Combining Medicinal Plant In Vitro Culture with Machine Learning Technologies for Maximizing the Production of Phenolic Compounds

Antioxidants ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 210 ◽  
Author(s):  
Pascual García-Pérez ◽  
Eva Lozano-Milo ◽  
Mariana Landín ◽  
Pedro Pablo Gallego
Keyword(s):  
Machine Learning ◽  
Phenolic Compounds ◽  
In Vitro Culture ◽  
Culture Media ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Starting Point ◽  
Plant In Vitro Culture

We combined machine learning and plant in vitro culture methodologies as a novel approach for unraveling the phytochemical potential of unexploited medicinal plants. In order to induce phenolic compound biosynthesis, the in vitro culture of three different species of Bryophyllum under nutritional stress was established. To optimize phenolic extraction, four solvents with different MeOH proportions were used, and total phenolic content (TPC), flavonoid content (FC) and radical-scavenging activity (RSA) were determined. All results were subjected to data modeling with the application of artificial neural networks to provide insight into the significant factors that influence such multifactorial processes. Our findings suggest that aerial parts accumulate a higher proportion of phenolic compounds and flavonoids in comparison to roots. TPC was increased under ammonium concentrations below 15 mM, and their extraction was maximum when using solvents with intermediate methanol proportions (55–85%). The same behavior was reported for RSA, and, conversely, FC was independent of culture media composition, and their extraction was enhanced using solvents with high methanol proportions (>85%). These findings confer a wide perspective about the relationship between abiotic stress and secondary metabolism and could serve as the starting point for the optimization of bioactive compound production at a biotechnological scale.

scholarly journals In Vitro Antioxidant Activity of Dibenz[b,f]azepine and its Analogues

2008 ◽  
Vol 5 (s2) ◽  
pp. 1123-1132 ◽  
Author(s):  
H. Vijay Kumar ◽  
C. R. Gnanendra ◽  
Nagaraja Naik ◽  
D. Channe Gowda
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
In Vitro Models ◽  
Antioxidant Compounds ◽  
Dependent Manner ◽  
Concentration Dependent Manner

Dibenz[b,f]azepine and its five derivatives bearing different functional groups were synthesized by known methods. The compounds thus synthesized were evaluated for antioxidant potential through different in vitro models such as (DPPH) free radical scavenging activity,ß-carotene-linoleic acid model system, reducing power assay and phosphomolybdenum method. Under our experimental condition among the synthesized compounds dibenz[b,f]azepine (a) and 10-methoxy-5H-dibenz[b,f]azepine (d) exhibited potent antioxidant activity in concentration dependent manner in all the above four methods. Butylated hydroxyl anisole (BHA) and ascorbic acid (AA) were used as the reference antioxidant compounds. The most active compounds like dibenz[b,f]azepine and its methoxy group substituent have shown more promising antioxidant and radical scavengers compared to the standards like BHA and ascorbic acid. It is conceivable from the studies that the tricyclic amines,i.e. dibenz[b, f]azepine and some of its derivatives are effective in their antioxidant activity properties.

scholarly journals Ultrafine Resveratrol Particles: Supercritical Antisolvent Preparation and Evaluation In Vitro and In Vivo

2015 ◽  
Vol 2015 ◽  
pp. 1-10
Author(s):  
Kunlun Wang ◽  
Xiuhua Zhao ◽  
Yuangang Zu ◽  
Jialei Li ◽  
Xiaonan Zhang ◽  
...  
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Degree Of Crystallinity ◽  
Optimum Conditions ◽  
Supercritical Antisolvent ◽  
Preparation And Evaluation ◽  
The Degree Of Crystallinity

Ultrafine resveratrol (u-Res) particles were prepared through the SAS process. The orthogonal method was used to optimize the factors of the SAS process. The size of u-Res reached 0.68 μm under the optimum conditions. The characterization of the u-Res particles was tested by many analysis methods. The chemical structure of Res was unaffected by the SAS process. The degree of crystallinity of the u-Res particles greatly reduced. The purity of the u-Res particles increased from 98.5% to 99.2% during the SAS process. The u-Res particles had greater saturation solubility and dissolution rate than the raw-Res (r-Res) particles. The radical scavenging activity and bioavailability of the u-Res in vivo were 1.9 times of the r-Res.

scholarly journals Antioxidant Activity and Bioactive Compounds of Lamium album Flower Extracts Obtained by Supercritical Fluid Extraction

2021 ◽  
Vol 11 (16) ◽  
pp. 7419
Author(s):  
Pascaline Aimee Uwineza ◽  
Anna Gramza-Michałowska ◽  
Marcin Bryła ◽  
Agnieszka Waśkiewicz
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Array Detector ◽  
Frap Assay ◽  
Constant Flow Rate ◽  
Lamium Album

In this research, supercritical CO2 extraction is applied to extract bioactive compounds from Lamium album (white dead nettle, Lamiaceae). Extraction was performed at various temperatures (40, 50, and 60 °C) using methanol as co-solvent at a constant flow rate of CO2, methanol, and pressure. The collected extracts were characterized in terms of antioxidant capacity by using DPPH, ABTS and FRAP in vitro antioxidant activity assays, whereas the Folin–Ciocalteu procedure was employed to estimate the total phenols content (TPC). On the other hand, phenolic compounds in the extracts were quantitated by liquid chromatography coupled with a photodiode array detector (UPLC-PDA) and confirmed with a mass detector (TQD). The extracts have shown high TPC ranged between 234.17 to 650.17 mg GAE/g extract. DPPH scavenging of the extracts was estimated and obtained EC50 values ranged from 0.12 to 0.37 mg/mL of solution. The ABTS radical scavenging activity ranged from 43.20 to 44.53 µg TE/g. The FRAP value was found within the range of 19.48 to 44.74 µmol TE/g of extract. Differences between extraction conditions were observed. In this research, 50 °C/250 bar was efficient for the TPC, DPPH, ABTS, and FRAP assays; moreover, statistically, TPCs and FRAP assay showed significant differences between the conditions at α = 0.05. The identification of phenolic compounds in the obtained extract of Lamium album flowers, using UPLC/PDA, revealed that chrysin, pinostrobin, myricetin, and trans-3-hydroxycinnamic acid were the significant molecules present, which may be responsible for the high content of polyphenols and antioxidant activity. The results obtained indicated that SC-CO2 could be considered an alternative method for extracting bioactive compounds of Lamium album. High antioxidant activity and the presence of various bioactive compounds indicate the potential of this plant from the Lamiaceae family and the possibility of its application in various industries, including agriculture, food technology, or pharmacy.

DETERMINATION OF BIOACTIVE CONTENTS AND IN VITRO ANTIOXIDANT ACTIVITY OF POLY HERBAL FORMULATIONS

INDIAN DRUGS ◽  
2020 ◽  
Vol 57 (05) ◽  
pp. 74-79
Author(s):  
A. K Srivastava ◽  
D. Kaushik ◽  
V. K. Lal
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
In Vitro Models ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Cardiac Complications ◽  
Scavenging Activity

Free radicals are reactive molecules involved in many physiological processes and have been associated with many diseases, such as ageing, cancer, arthritis and liver injury and cardiac complications. In polyherbal formulations HAF-I and HAF-II, described below, the total phenolics content were found to be 34.4±0.10 and 27.6±1.20 mg gallic acid equivalent (GAE)/g and total flavonoids contents, total tannin contents were 24.7±0.25 and 18.1±1.20 RE/g and 12.31±0.25 and 9.48±1.85 GAE/g, respectively. Free radical scavenging activity was determined according to the elimination of DPPH radicals. Total phenol content was determined by the Folin–Ciocalteu reaction. The relative antioxidant ability of the polyherbal formulations were investigated through two in vitro models, namely, antioxidant capacity by radical scavenging activity using α, α-diphenyl-β-picrylhydrazyl (DPPH) and nitric oxide (NO) methods. The extracts were used at 20, 40, 60, 80 and 100 μg/mL concentrations and radical scavenging activity was determined in terms of inhibition percentage. The IC50 (concentration required for 50% inhibition) were calculated for each radical. The present study was designed to evaluate the free radical scavenging activity of hydro-alcoholic extracts of polyherbal formulations (HAF-I & HAF-II) various in-vitro models using ascorbic acid and rutin as references. The in vitro free radical DPPH activities were found to be 74.17±0.18 & 75.30±0.18 and NO antioxidant activity were found to be 75.3±1.10 & 76.17±1.24 at maximum concentration of 100 μg/mL. The in-vitro anti-oxidant activity of these polyherbal formulations may be due to the presence of polyphenols.

DETERMINATION OF BIOACTIVE CONTENTS AND IN VITRO ANTIOXIDANT ACTIVITY OF POLY HERBAL FORMULATIONS

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (06) ◽  
pp. 56-62
Author(s):  
A. K Srivastava ◽  
◽  
D. Kaushik ◽  
V. K. Lal ◽  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
In Vitro Models ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Cardiac Complications ◽  
Scavenging Activity

Free radicals are reactive molecules involved in many physiological processes and have been associated with many diseases, such as ageing, cancer, arthritis and liver injury and cardiac complications. The total phenolics content were found to be 34.4±0.10 and 27.6±1.20 mg gallic acid equivalent (GAE)/g and total flavonoids contents, total tannin contents were 24.7±0.25 & 18.1±1.20 RE/g and 12.31±0.25 & 9.48±1.85 GAE/g, respectively of polyherbal formulations (HAF-I & HAF-II). Free radical scavenging activity was determined according to the elimination of DPPH radicals and total phenol content was determined by the Folin–Ciocalteu reaction. The relative antioxidant ability of the polyherbal formulations were investigated through two in vitro models, such as antioxidant capacity by radical scavenging activity using, α, α-diphenyl-β-picrylhydrazyl (DPPH) and nitric oxide (NO) methods. The extracts were used at concentration 20, 40, 60, 80 and 100 μg/mL concentrations and radical scavenging activity was determined in terms of inhibition percentage. The IC50 (concentration required for 50% inhibition) were calculated for each radicals. The present study was designed to evaluate the free radical scavenging activity of hydro-alcoholic extracts of polyherbal formulations (HAF-I & HAF-II) various in vitro models using Ascorbic acid and Rutin as a reference. The In vitro free radical DPPH activities were found to 74.17±0.18 & 75.30±0.18 and NO antioxidant activity were found to 75.3±1.10 & 76.17±1.24 at maximum concentration of 100 μg/mL. The In vitro anti-oxidant activity of these polyherbal formulations may be due to the presence of polyphenols.

Phytochemical Investigation of Phenolic Constituents and in vitro Evaluation of Antioxidant Activity of Five Algerian Propolis

2020 ◽  
Vol 16 ◽  
Author(s):  
Amina Bouaroura ◽  
Narimane Segueni ◽  
Ramazan Erenler ◽  
Abdghani May ◽  
Chawki Bensouici ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
Biological Effects ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Phenolic Contents ◽  
Phenolic Constituents

Background:: Algerian propolis has gained interest in the last recent years. Many researches concerning both its biological effects and chemical composition were performed. Objective:: The present study was designed to investigate the chemical composition in particular phenolic compounds and the antioxidant activity of five Algerian propolis collected from different geographical parts of Algerian north namely: Constantine (CN), Boumerdes (BN), Mila (MN), Tebessa (TN) and Guelma (GN). Method:: Propolis extracts were obtained using solvents of varying polarity: petroleum ether, chloroform, ethyl acetate and finally methanol. Phenolic compounds were determined by liquid chromatography-tandem mass spectrometry (LCMS/ MS). In addition, total flavonoid and phenolic contents were also determined. Antioxidant activity was investigated using five complementary tests namely: DPPH., ABTS.+ assays for radical-scavenging activity, β-carotene-linoleic acid assay for lipid peroxidation activity, CUPRAC and FRAP assays for reduction capacity. Results:: The main phenolic compounds detected in the present study were: caffeic, p-coumaric, cinnamic and chlorogenic acids as well as naringenin and kampferol. Among the tested extracts, ethyl acetate and methanol extracts exhibited the highest phenolic and flavonoid contents and the strongest antioxidant activity. Propolis of Constantine was the most active one. Conclusion:: Our results suggest a potential use of Algerian propolis as a natural source of bioactive compounds with antioxidant activity.

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