Antioxidant Status Modifications by Topical Administration of Dorzolamide in Primary Open-Angle Glaucoma

2009 ◽  
Vol 19 (4) ◽  
pp. 565-571 ◽  
Author(s):  
Vicente Zanon-Moreno ◽  
Jose J. Garcia-Medina ◽  
Roberto Gallego-Pinazo ◽  
Ignacio Vinuesa-Silva ◽  
Maria A. Moreno-Nadal ◽  
...  
Keyword(s):  
Aqueous Humor ◽  
Antioxidant Status ◽  
Open Angle Glaucoma ◽  
Total Antioxidant Status ◽  
Thiobarbituric Acid ◽  
Topical Administration ◽  
Oxidative Activity ◽  
Sod Activity ◽  
Total Antioxidant ◽  
Comparative Group

Purpose This study deals with the role of the topical administration of dorzolamide eyedrops on the oxidative/antioxidant status of aqueous humor in patients with primary openangle glaucoma (POAG). Methods A case-control study including 130 patients distributed into three groups was carried out: 1) patients with POAG without dorzolamide instillation administration (GG; n=34); 2) patients with POAG with dorzolamide (DG; n=36); and 3) subjects with cataracts (comparative group, CG; n=60). Oxidative activity was measured in the aqueous humor by malondialdehyde determination by the thiobarbituric acid reactive substances assay. Antioxidant status was assessed in the aqueous humor samples by measuring the superoxide dismutase activity and the total antioxidant status. Results Oxidative activity was significantly higher in both glaucoma groups than in the cataracts group (GG vs CG, p=3.68 E-34; DG vs CG, p=5.11 E-45), and was significantly higher in GG than in DG (p=0.0034). SOD activity was significantly higher in both glaucoma groups than in the cataracts group (GG vs CG, p=1.08 E-14; DG vs CG, p=3.70 E-22), and was significantly higher in GG than in DG (p=0.018). Finally, total antioxidant status was significantly decreased in both glaucoma groups compared with the cataracts group (GG vs CG, p=2.51 E-12; DG vs CG, p=5.06 E-05), and was more significantly decreased in GG than in DG (p=9.23 E-07). Conclusions Topical administration of dorzolamide colirium diminishes oxidative stress in patients with glaucoma.

scholarly journals Are insulin-resistance and oxidative stress cause or consequence of aging

2020 ◽  
Vol 245 (14) ◽  
pp. 1260-1267
Author(s):  
Sylwia Dzięgielewska-Gęsiak ◽  
Dorota Stołtny ◽  
Alicja Brożek ◽  
Małgorzata Muc-Wierzgoń ◽  
Ewa Wysocka
Keyword(s):  
Oxidative Stress ◽  
Insulin Resistance ◽  
Antioxidant Status ◽  
Antioxidant Defense ◽  
Total Antioxidant Status ◽  
Thiobarbituric Acid ◽  
Elderly Individuals ◽  
Total Antioxidant ◽  
Antioxidant Defense Systems ◽  
Antioxidant Markers

Insulin resistance (IR) may be associated with oxidative stress and leads to cardiovascular disorders. Current research focuses on interplay between insulin-resistance indices and oxidant-antioxidant markers in elderly individuals with or without insulin-resistance. The assessment involved anthropometric data (weight, height, BMI, percentage of body fat (FAT)) and biochemical tests (glucose, lipids, serum insulin and plasma oxidant-antioxidant markers: Thiobarbituric Acid-Reacting Substances (TBARS), Cu,Zn-superoxide dismutase (SOD-1) and total antioxidant status). Insulin resistance index (IR) assuming a cut-off point of 0.3 allows to divides groups into: insulin sensitive group (InsS) IR < 0,3 ( n = 35, median age 69.0 years) and insulin-resistant group (InsR) IR ≥ 0.3 ( n = 51, median age 71.0 years). Lipids and antioxidant defense system markers did not differentiate the investigated groups. In the InsR elderly group, the FAT was increased ( P < 0.000003) and TBARS ( P = 0.008) concentration decreased in comparison with InsS group. A positive correlation for SOD-1 and total antioxidant status ( P < 0.05; r =  0.434) and a negative correlation for TBARS and age ( P < 0.05 with r = −0.421) were calculated in InsR individuals. In elderly individuals, oxidative stress persists irrespective of insulin-resistance status. We suggest that increased oxidative stress may be consequence of old age. An insulin action identifies those at high risk for atherosclerosis, via congruent associations with oxidative stress and extra- and intra-cellular antioxidant defense systems. Thus, we maintain that insulin-resistance is not the cause of aging. Impact statement Insulin resistance is associated with oxidative stress leading to cardiovascular diseases. However, little research has been performed examining elderly individuals with or without insulin-resistance. We demonstrate that antioxidant defense systems alone is not able to abrogate insulin action in elderly individuals at high risk for atherosclerosis, whereas the combined oxidant-antioxidant markers (thiobarbituric acid-reacting substances (TBARS), Cu,Zn-superoxide dismutase (SOD-1), and total antioxidant status (TAS)) might be more efficient and perhaps produce better clinical outcome. In fact, a decrease in oxidative stress and strong interaction between antioxidant defense can be seen only among insulin-resistant elderly individuals. This is, in our opinion, valuable information for clinicians, since insulin-resistance is considered strong cardiovascular risk factor.

Protective effects of vitamin E against CCl4-induced hepatotoxicity in rabbits

2007 ◽  
Vol 61 (1) ◽  
Author(s):  
K. Pawłowska-Góral ◽  
M. Wardas ◽  
P. Wardas ◽  
A. Rzepecka-Stojko
Keyword(s):  
Vitamin E ◽  
Antioxidant Status ◽  
Superoxide Radical ◽  
Malonic Dialdehyde ◽  
Total Antioxidant Status ◽  
Protective Effects ◽  
Sod Activity ◽  
Superoxide Radical Anion ◽  
Total Antioxidant ◽  
Reactive Oxygen Forms

AbstractThe influence of CCl4 on the activity of superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), the value of the total antioxidant status (TAS), and the concentration of malonic dialdehyde (MDA) and glutathione (GSH) was monitored in plasma or whole blood of rabbits. The administration of CCl4 caused the increase of the SOD activity to approximately 150 % and the decrease in the activity of GPx and GR by about 50 %. These changes were accompanied with the increase in TAS value and MDA concentration and the decrease of GSH concentration. The effect of CCl4 was suppressed by the previous 7 days lasting or simultaneous administration of vitamin E. Oxidative stress caused by CCl4 was accompanied by the development of reactive oxygen forms, especially superoxide radical anion.

scholarly journals Studies on the biological effects of ozone: 8. Effects on the total antioxidant status and on interleukin-8 production

1998 ◽  
Vol 7 (5) ◽  
pp. 313-317 ◽  
Author(s):  
V. Bocci ◽  
G. Valacchi ◽  
F. Corradeschi ◽  
G. Fanetti
Keyword(s):  
Antioxidant Status ◽  
Cytokine Production ◽  
Biological Effects ◽  
Total Antioxidant Status ◽  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substances ◽  
Protein Thiol ◽  
Cytokine Induction ◽  
Wide Range ◽  
Total Antioxidant

Ozone (O3) is a controversial gas because, owing to its potent oxidant properties, it exerts damaging effects on the respiratory tract and yet it has been used for four decades as a therapy. While the disinfectant activity of O3is understandable, it is less clear how other biological effects can be elicited in human blood with practically no toxicity. On the other hand plasma and cells are endowed with a powerful antioxidant system so that a fairly wide range of O3concentrations between 40 and 80μ g/ml per gram of blood (˜0.83-1.66 mM) are effective but not deleterious. After blood ozonation total antioxidant status (TAS) and plasma protein thiol groups (PTG) decrease by 20% and 25%, respectively, while thiobarbituric acid reactive substances (TBARS) increases up to fivefold. The increase of haemolysis is negligible suggesting that the erythrocyte membrane is spared at the expense of other sacrificial substrates. While there is a clear relationship between the ozone dose and IL-8 levels, we have noticed that high TAS and PTG values inhibit the cytokine production. This is in line with the current idea that hydrogen peroxide, as a byproduct of O3decomposition, acts as a messenger for the cytokine induction.

scholarly journals Antioxidant Action of Solid Preparation of Xingnaojing in SHRSP

2013 ◽  
Vol 8 (5) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Yang Liu ◽  
Naomi Yasui ◽  
Aya Kishimoto ◽  
Jian-ning Sun ◽  
Katsumi Ikeda
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Thiobarbituric Acid ◽  
Antioxidant Effect ◽  
Antioxidant Action ◽  
Thiobarbituric Acid Reactive Substances ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Total Antioxidant

We investigated the antioxidant action of a solid preparation of Xingnaojing (XNJ) and ascorbic acid (AA) in stroke-prone spontaneously hypertensive rats (SHRSP). The total antioxidant states in the plasma, systolic blood pressure, and heart rate were measured every 2 weeks, and lipid peroxidation, expressed as thiobarbituric acid-reactive substances in plasma, was measured in the 6th week. The results showed that AA and XNJ significantly increased the total antioxidant status in plasma and reduced malondialdehyde in the plasma. These data suggest that during 6-week administration, XNJ has antioxidant action on SHRSP, which may relate to its generalized inhibition of lipid peroxidation and promotion of the total antioxidant state. These results demonstrated that orally treated XNJ has an antioxidant effect on SHRSP plasma.

scholarly journals Positive Relationship between Total Antioxidant Status and Chemokines Observed in Adults

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Yanli Li ◽  
Richard W. Browne ◽  
Matthew R. Bonner ◽  
Furong Deng ◽  
Lili Tian ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Status ◽  
Adult Population ◽  
Total Antioxidant Status ◽  
Thiobarbituric Acid ◽  
Oxidative Stress Biomarkers ◽  
Cross Sectional ◽  
Drinking Status ◽  
Total Antioxidant ◽  
Positive Correlations

Objective. Human evidence is limited regarding the interaction between oxidative stress biomarkers and chemokines, especially in a population of adults without overt clinical disease. The current study aims to examine the possible relationships of antioxidant and lipid peroxidation markers with several chemokines in adults.Methods. We assessed cross-sectional associations of total antioxidant status (TAS) and two lipid peroxidation markers malondialdehyde (MDA) and thiobarbituric acid reactive substances (TBARS) with a suite of serum chemokines, including CXCL-1 (GRO-α), CXCL-8 (IL-8), CXCL-10 (IP-10), CCL-2 (MCP-1), CCL-5 (RANTES), CCL-8 (MCP-2), CCL-11 (Eotaxin-1), and CCL-17 (TARC), among 104 Chinese adults without serious preexisting clinical conditions in Beijing before 2008 Olympics.Results. TAS showed significantly positive correlations with MCP-1 (r=0.15751,P=0.0014), MCP-2 (r=0.3721,P=0.0001), Eotaxin-1 (r=0.39598,P<0.0001), and TARC (r=0.27149,P=0.0053). The positive correlations remained unchanged after controlling for age, sex, body mass index, smoking, and alcohol drinking status. No associations were found between any of the chemokines measured in this study and MDA or TBARS. Similar patterns were observed when the analyses were limited to nonsmokers.Conclusion. Total antioxidant status is positively associated with several chemokines in this adult population.

scholarly journals Study of the Correlations among Some Parameters of the Oxidative Status, Gelatinases, and Their Inhibitors in a Group of Subjects with Metabolic Syndrome

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
E. Hopps ◽  
R. Lo Presti ◽  
M. Montana ◽  
B. Canino ◽  
M. R. Averna ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Metabolic Syndrome ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Thiobarbituric Acid ◽  
Oxidative Status ◽  
Entire Group ◽  
Carbonyl Groups ◽  
Total Antioxidant ◽  
Nitric Oxide Metabolites

Our aim was to examine some parameters of oxidative status, gelatinases, and their inhibitors and to evaluate their interrelationships in subjects with metabolic syndrome (MS). We enrolled 65 MS subjects, subdivided according to the presence or not of diabetes mellitus. We examined lipid peroxidation (expressed as thiobarbituric acid reacting substances, TBARS), protein oxidation (expressed as carbonyl groups), nitric oxide metabolites (NOx), total antioxidant status (TAS), MMP-2, MMP-9, TIMP-1, and TIMP-2. We found that MS subjects, diabetics and nondiabetics, showed an increase in TBARS, PC, and NOx. A significant decrease in TAS was observed only in nondiabetic MS subjects in comparison with diabetic MS subjects. We observed increased concentrations of MMP-2, MMP-9, TIMP-1, and TIMP-2, higher in diabetic subjects. Our data showed a positive correlation between TAS and MMP-2, TAS and MMP-9, and TAS and MMP-9/TIMP-1 and a negative correlation between TBARS and MMP-2 in diabetic MS subjects in the entire group. In MS subjects a prooxidant status and increased levels of gelatinases and their inhibitors are evident although the correlations between oxidative stress and MMPs or TIMPs are controversial and need further investigation.

Serum malondialdehyde level and activity of total antioxidant status of dogs with age-related cataract

2016 ◽  
Vol 19 (2) ◽  
pp. 429-431 ◽  
Author(s):  
J. Madany
Keyword(s):  
Antioxidant Status ◽  
Colorimetric Method ◽  
Total Antioxidant Status ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Study Objective ◽  
Age Related ◽  
Significant Difference ◽  
Total Antioxidant ◽  
Cataract Development

Abstract Study objective: determination of malondialdehyde (MDA) level and total antioxidant status (TAS) in the serum of dogs with age-related cataract. Material and methods: Forty dogs, 10-16 years of age, which were diagnosed with age-related cataract were examined. The control group consisted of 12 dogs, 8-13 years of age, without cataract. MDA was determined using a Perkin-Elmer LS 30 luminescence spectrometer using the method with thiobarbituric acid (TBA). TAS was determined using the colorimetric method with a set of Randox Total Antioxidant Status reagents. Results: The results show a higher level of MDA with statistically significant difference and lower activity of TAS in the serum of dogs with age-related cataract. Conclusions: The results obtained indicate higher systemic lipid peroxidation and weakening of the general defense system in dogs with age-related cataract. These results suggest that such conditions may have an effect on the development of age-related cataract and are consistent with the theory of free radical age-related cataract development.

scholarly journals Tai Chi Exercise Increases SOD Activity and Total Antioxidant Status in Saliva and Is Linked to an Improvement of Periodontal Disease in the Elderly

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Víctor Manuel Mendoza-Núñez ◽  
Beatriz Hernández-Monjaraz ◽  
Edelmiro Santiago-Osorio ◽  
José Miguel Betancourt-Rule ◽  
Mirna Ruiz-Ramos
Keyword(s):  
Older Adults ◽  
Periodontal Disease ◽  
Antioxidant Status ◽  
Tai Chi ◽  
Total Antioxidant Status ◽  
The Elderly ◽  
Control Group ◽  
Sod Activity ◽  
Spectrophotometric Methods ◽  
Total Antioxidant

The aim of this study was to determine the effect of Tai Chi on biological markers of oxidative stress in saliva and its relationship with periodontal disease (PD) in older adults. We carried out a quasi-experimental study with a sample of 71 sedentary volunteers with PD who were divided into a control group of 34 subjects and an experimental group of 37 subjects who performed Tai Chi 5 days a week for a period of 6 months. PD status was characterized using the Periodontal Disease Index (PDI). Superoxide dismutase (SOD), total antioxidant status (TAS), and TBARS levels of both groups were measured by spectrophotometric methods. In addition, inflammation markers (TNF-α, IL-1β, IL-6, IL-8, and IL-10) were measured by flow cytometry. We found a statistically significant increase in SOD activity (P<0.001) and TAS concentration (P<0.05), whereas levels of IL-1βwere significantly lower (P<0.01). Likewise, a statistically significant decrease in the PDI (P<0.05) was observed in subjects who performed Tai Chi during a period of 6 months. Our findings suggest that the practice of Tai Chi has both antioxidant and anti-inflammatory effects that are linked to the improvement of PD in older adults.

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