Serum malondialdehyde level and activity of total antioxidant status of dogs with age-related cataract

2016 ◽  
Vol 19 (2) ◽  
pp. 429-431 ◽  
Author(s):  
J. Madany
Keyword(s):  
Antioxidant Status ◽  
Colorimetric Method ◽  
Total Antioxidant Status ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Study Objective ◽  
Age Related ◽  
Significant Difference ◽  
Total Antioxidant ◽  
Cataract Development

Abstract Study objective: determination of malondialdehyde (MDA) level and total antioxidant status (TAS) in the serum of dogs with age-related cataract. Material and methods: Forty dogs, 10-16 years of age, which were diagnosed with age-related cataract were examined. The control group consisted of 12 dogs, 8-13 years of age, without cataract. MDA was determined using a Perkin-Elmer LS 30 luminescence spectrometer using the method with thiobarbituric acid (TBA). TAS was determined using the colorimetric method with a set of Randox Total Antioxidant Status reagents. Results: The results show a higher level of MDA with statistically significant difference and lower activity of TAS in the serum of dogs with age-related cataract. Conclusions: The results obtained indicate higher systemic lipid peroxidation and weakening of the general defense system in dogs with age-related cataract. These results suggest that such conditions may have an effect on the development of age-related cataract and are consistent with the theory of free radical age-related cataract development.

scholarly journals Relationship between Estradiol and Antioxidant Enzymes Activity of Ischemic Stroke

2009 ◽  
Vol 2009 ◽  
pp. 1-5 ◽  
Author(s):  
Nasrin Sheikh ◽  
Heidar Tavilani ◽  
Aliakbar Rezaie ◽  
Asad Vaisi-raygani ◽  
Saeedeh Salimi
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Ischemic Stroke ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Control Group ◽  
Significant Difference ◽  
Total Antioxidant ◽  
And Control

Some evidence suggests the neuroprotection of estrogen provided by the antioxidant activity of this compound. The main objective of this study was to determine the level of estradiol and its correlation with the activity of antioxidant enzymes, total antioxidant status and ferritin from ischemic stroke subjects. The study population consisted of 30 patients with acute ischemic stroke and 30 controls. There was no significant difference between estradiol in stroke and control group. The activity of superoxide dismutase and level of ferritin was higher in stroke compared with control group (P<.05,P<.001, resp.). There was no significant correlation between estradiol and glutathione peroxidase, glutathione reductase, catalase, total antioxidant status, and ferritin in stroke and control groups. We observed inverse correlation between estradiol with superoxide dismutase in males of stroke patients (r=−0.54,P=.029). Our results supported that endogenous estradiol of elderly men and women of stroke or control group has no antioxidant activity.

scholarly journals Oxidative Stress and Antioxidant Status in Patients with Bell’s Palsy

2017 ◽  
Vol 36 (1) ◽  
pp. 18-22 ◽  
Author(s):  
Suat Terzi ◽  
Engin Dursun ◽  
Adnan Yılmaz ◽  
Zerrin Özergin Coşkun ◽  
Abdulkadir Özgür ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Bell’S Palsy ◽  
Stress Index ◽  
Control Group ◽  
Bell's Palsy ◽  
Significant Difference ◽  
Total Antioxidant ◽  
High Oxidative Stress

SummaryBackground:Bell’s palsy (BP) is the most common acute mononeuropathy of unilateral facial paralysis. Immune, infective and ischaemic mechanisms are potential contributors to the development of BP, but the precise cause remains unclear. Recently, oxidative stress has been proposed as a risk factor of various idiopathic diseases. The aim of this study was to investigate the possible role of oxidative stress in patients with BP.Methods:Thirty-two patients with BP and 30 healthy controls were included in this study. Serum total oxidant status (TOS), total antioxidant status (TAS) and oxidative stress index (OSI) were measured by the Erel method.Results:Serum TOS activities and OSI values were significantly higher in patients with BP compared with the control group (P<0.003 for all comparisons), whereas there was no significant difference between the groups in terms of TAS levels (P>0.05).Conclusions:The data suggest that oxidative stress is increased in BP. These results of high oxidative stress in patients with BP may be helpful to clarify the etiopathogenesis of BP and contribute to improvement in the management or prevention of the disease.

scholarly journals Aloe vera extract reduces 8-oxo-2'-deoxyguanosine levels and improves total antioxidants in streptozotocin-induced diabetic rats

2017 ◽  
Vol 36 (1) ◽  
pp. 34
Author(s):  
Wulan Christijanti ◽  
Aditya Marianti ◽  
Wiwi Isnaeni
Keyword(s):  
Antioxidant Status ◽  
Aloe Vera ◽  
Total Antioxidant Status ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Control Group ◽  
Treatment Groups ◽  
Rat Testis ◽  
Significant Difference ◽  
Total Antioxidant

<p><strong>Background</strong></p><p>Diabetes mellitus is a chronic disease caused by lack of insulin production in the pancreas or by insulin resistance, the disease being characterized by elevated blood glucose levels. Hyperglycemia in diabetes could lead to oxidative stress due to the rise in 8-oxo-2'-deoxyguanosine (8-oxo-dG) levels and the decrease in levels of total antioxidant status (TAS). The purpose of this study was to assess the effect <em>Aloe vera </em>extract on 8-oxo-dG level and total antioxidant status in diabetic rat testis.</p><p><strong> </strong></p><p><strong>Methods</strong></p><p>This was an experimental laboratory study with 20 rat samples which were divided into 4 groups (1 control group and 3 treatment groups). Diabetes was induced in the rats by streptozotocin (STZ) at 65 mg/kgBW. The diabetic rats were then treated for 28 days with <em>Aloe vera</em> extract at 0 mg (P0), 200 mg rind (P1), 200 mg pulp (P2), respectively. The level of 8 -oxo-dG was measured by ELISA and total antioxidant status with 2,2' -azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS). Data were analyzed with ANOVA and Least Significant Difference Advanced Test at P&lt;0.05.</p><p><strong> </strong></p><p><strong>Results</strong></p><p>8-Oxo-dG levels were significantly different between the control group and both P0 and P2, but not between the control group and P1. Among the treatment groups the 8-oxo-dG levels were significantly different. Mean total antioxidant status was significantly different between control and treatment groups, and also between treatment groups (p&lt;0.05).</p><p><strong> </strong></p><p><strong>Conclusions</strong></p><p><em>Aloe vera</em> extract reduced free radicals (level of 8-oxo-dG) and increased the total antioxidant status in diabetic rat testis.</p><p> </p>

Antioxidant status of blood in patients with vibration disease

2019 ◽  
pp. 978-982 ◽  
Author(s):  
N. N. Malyutina ◽  
A. F. Bolotova ◽  
R. B. Eremeev ◽  
A. Zh. Gilmanov ◽  
D. Yu. Sosnin
Keyword(s):  
Antioxidant Activity ◽  
Blood Serum ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Main Group ◽  
Biologically Active ◽  
Antioxidant Systems ◽  
Control Group ◽  
Vibration Disease ◽  
Total Antioxidant

Introduction. The overwhelming number of publications contains only data on the content of individual antioxidants, but not on the overall antioxidant activity of the blood in patients with vibration disease.The aim of the study was to determine the total antioxidant activity of blood serum in patients with vibration disease.Materials and methods. Th e main group consisted of 30 people diagnosed with “Vibration disease” of 1 degree (n=21) and 2 degrees (n=9). Th e control group consisted of 30 clinically healthy men, comparable in age with the main group (p=0.66). Th e total activity of antioxidant systems of blood plasma was evaluated photometrically using the test system “Total antioxidant status-Novo” (“Vector-best”, Russia).Results. The indicator of the total antioxidant status (TAS) was 1,038±0.232 mmol/l in the examined main group, against 1,456±0.225 mmol/l in the examined control group (p<0.000001). Th e coefficient of variation (CV) in patients with vibration disease was 22.35%, 1.45 times higher than in the control group (15.45%). In the main group there was a positive correlation between age and TAS (R=0.525), in the control group there was no such relationship (R=0.095). Th e degree of decrease depended on the severity of vibration disease.Conclusions. 1. The development of vibration disease is accompanied by a decrease in the antioxidant status of blood serum. 2. Th e degree of decrease in the antioxidant status of blood serum correlates with the severity of vibration disease. 3. Reduction of TAS can serve as a pathogenetic justification of the need to include drugs and/or biologically active additives with antioxidant activity in therapy

scholarly journals Are insulin-resistance and oxidative stress cause or consequence of aging

2020 ◽  
Vol 245 (14) ◽  
pp. 1260-1267
Author(s):  
Sylwia Dzięgielewska-Gęsiak ◽  
Dorota Stołtny ◽  
Alicja Brożek ◽  
Małgorzata Muc-Wierzgoń ◽  
Ewa Wysocka
Keyword(s):  
Oxidative Stress ◽  
Insulin Resistance ◽  
Antioxidant Status ◽  
Antioxidant Defense ◽  
Total Antioxidant Status ◽  
Thiobarbituric Acid ◽  
Elderly Individuals ◽  
Total Antioxidant ◽  
Antioxidant Defense Systems ◽  
Antioxidant Markers

Insulin resistance (IR) may be associated with oxidative stress and leads to cardiovascular disorders. Current research focuses on interplay between insulin-resistance indices and oxidant-antioxidant markers in elderly individuals with or without insulin-resistance. The assessment involved anthropometric data (weight, height, BMI, percentage of body fat (FAT)) and biochemical tests (glucose, lipids, serum insulin and plasma oxidant-antioxidant markers: Thiobarbituric Acid-Reacting Substances (TBARS), Cu,Zn-superoxide dismutase (SOD-1) and total antioxidant status). Insulin resistance index (IR) assuming a cut-off point of 0.3 allows to divides groups into: insulin sensitive group (InsS) IR < 0,3 ( n = 35, median age 69.0 years) and insulin-resistant group (InsR) IR ≥ 0.3 ( n = 51, median age 71.0 years). Lipids and antioxidant defense system markers did not differentiate the investigated groups. In the InsR elderly group, the FAT was increased ( P < 0.000003) and TBARS ( P = 0.008) concentration decreased in comparison with InsS group. A positive correlation for SOD-1 and total antioxidant status ( P < 0.05; r =  0.434) and a negative correlation for TBARS and age ( P < 0.05 with r = −0.421) were calculated in InsR individuals. In elderly individuals, oxidative stress persists irrespective of insulin-resistance status. We suggest that increased oxidative stress may be consequence of old age. An insulin action identifies those at high risk for atherosclerosis, via congruent associations with oxidative stress and extra- and intra-cellular antioxidant defense systems. Thus, we maintain that insulin-resistance is not the cause of aging. Impact statement Insulin resistance is associated with oxidative stress leading to cardiovascular diseases. However, little research has been performed examining elderly individuals with or without insulin-resistance. We demonstrate that antioxidant defense systems alone is not able to abrogate insulin action in elderly individuals at high risk for atherosclerosis, whereas the combined oxidant-antioxidant markers (thiobarbituric acid-reacting substances (TBARS), Cu,Zn-superoxide dismutase (SOD-1), and total antioxidant status (TAS)) might be more efficient and perhaps produce better clinical outcome. In fact, a decrease in oxidative stress and strong interaction between antioxidant defense can be seen only among insulin-resistant elderly individuals. This is, in our opinion, valuable information for clinicians, since insulin-resistance is considered strong cardiovascular risk factor.

scholarly journals Studies on the biological effects of ozone: 8. Effects on the total antioxidant status and on interleukin-8 production

1998 ◽  
Vol 7 (5) ◽  
pp. 313-317 ◽  
Author(s):  
V. Bocci ◽  
G. Valacchi ◽  
F. Corradeschi ◽  
G. Fanetti
Keyword(s):  
Antioxidant Status ◽  
Cytokine Production ◽  
Biological Effects ◽  
Total Antioxidant Status ◽  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substances ◽  
Protein Thiol ◽  
Cytokine Induction ◽  
Wide Range ◽  
Total Antioxidant

Ozone (O3) is a controversial gas because, owing to its potent oxidant properties, it exerts damaging effects on the respiratory tract and yet it has been used for four decades as a therapy. While the disinfectant activity of O3is understandable, it is less clear how other biological effects can be elicited in human blood with practically no toxicity. On the other hand plasma and cells are endowed with a powerful antioxidant system so that a fairly wide range of O3concentrations between 40 and 80μ g/ml per gram of blood (˜0.83-1.66 mM) are effective but not deleterious. After blood ozonation total antioxidant status (TAS) and plasma protein thiol groups (PTG) decrease by 20% and 25%, respectively, while thiobarbituric acid reactive substances (TBARS) increases up to fivefold. The increase of haemolysis is negligible suggesting that the erythrocyte membrane is spared at the expense of other sacrificial substrates. While there is a clear relationship between the ozone dose and IL-8 levels, we have noticed that high TAS and PTG values inhibit the cytokine production. This is in line with the current idea that hydrogen peroxide, as a byproduct of O3decomposition, acts as a messenger for the cytokine induction.

scholarly journals Dietary Plant Extracts Improve the Antioxidant Reserves in Weaned Piglets

Antioxidants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 702
Author(s):  
Carlo Corino ◽  
Michel Prost ◽  
Barbara Pizzi ◽  
Raffaella Rossi
Keyword(s):  
Antioxidant Activity ◽  
Plant Extracts ◽  
Whole Blood ◽  
Antioxidant Status ◽  
Biological Activities ◽  
Total Antioxidant Status ◽  
Dietary Treatment ◽  
Control Group ◽  
Commercial Diet ◽  
Total Antioxidant

Reducing the use of antibiotics in livestock in order to contain antibiotic resistance and studying natural substance additives are key to sustainability. Among the various biological activities of plant extracts, antioxidant activity plays an important role. The present study assesses the total antioxidant activity and antioxidant reserves using the Kit Radicaux Libres test (KRL™ Kirial International, Couternon, France). One hundred and sixty piglets (Topics × Tempo) weaned at 28 days of age were divided into four dietary treatment groups that were fed a commercial diet (the control group, C); 500 mg/kg Boswellia extract (BOS); 200 and 50 mg/kg Uncaria and Tanacetum extracts (UT) respectively; and 225 mg/kg of an antioxidant plant extract mixture (AOX). The blood antioxidant activity of the piglets was measured using the KRL test and the reserves were analyzed on whole blood samples after hydrolysis with glucosidase, sulfatase and glucuronidase. No significant differences were observed in growth performance. The delta KRL values of the whole blood showed a significantly higher total antioxidant status of the piglets from the BOS and AOX groups than the UT and C groups (+30.7 BOS; +27.7 AOX vs. +17.81 UT +13.30 C; p = 0.002) between 18 and 28 days post-weaning. The delta KRL values of red blood cells (RBCs) showed a significantly higher total antioxidant status of the piglets from the AOX groups than the UT and BOS groups (+22.2 AOX; vs. +9.90 UT +9.4 BOS; p = 0.016) between the two sampling times. Reserves of UT and AOX were higher than C and BOS for all enzymes, glucosides, sulphates, and glucuronides. The biological KRL test proved to be an extremely sensitive tool to evaluate the piglets’ antioxidant status. Determining the antioxidant reserve also provides a better understanding of the real antioxidant status of pigs.

Antioxidant Status Modifications by Topical Administration of Dorzolamide in Primary Open-Angle Glaucoma

2009 ◽  
Vol 19 (4) ◽  
pp. 565-571 ◽  
Author(s):  
Vicente Zanon-Moreno ◽  
Jose J. Garcia-Medina ◽  
Roberto Gallego-Pinazo ◽  
Ignacio Vinuesa-Silva ◽  
Maria A. Moreno-Nadal ◽  
...  
Keyword(s):  
Aqueous Humor ◽  
Antioxidant Status ◽  
Open Angle Glaucoma ◽  
Total Antioxidant Status ◽  
Thiobarbituric Acid ◽  
Topical Administration ◽  
Oxidative Activity ◽  
Sod Activity ◽  
Total Antioxidant ◽  
Comparative Group

Purpose This study deals with the role of the topical administration of dorzolamide eyedrops on the oxidative/antioxidant status of aqueous humor in patients with primary openangle glaucoma (POAG). Methods A case-control study including 130 patients distributed into three groups was carried out: 1) patients with POAG without dorzolamide instillation administration (GG; n=34); 2) patients with POAG with dorzolamide (DG; n=36); and 3) subjects with cataracts (comparative group, CG; n=60). Oxidative activity was measured in the aqueous humor by malondialdehyde determination by the thiobarbituric acid reactive substances assay. Antioxidant status was assessed in the aqueous humor samples by measuring the superoxide dismutase activity and the total antioxidant status. Results Oxidative activity was significantly higher in both glaucoma groups than in the cataracts group (GG vs CG, p=3.68 E-34; DG vs CG, p=5.11 E-45), and was significantly higher in GG than in DG (p=0.0034). SOD activity was significantly higher in both glaucoma groups than in the cataracts group (GG vs CG, p=1.08 E-14; DG vs CG, p=3.70 E-22), and was significantly higher in GG than in DG (p=0.018). Finally, total antioxidant status was significantly decreased in both glaucoma groups compared with the cataracts group (GG vs CG, p=2.51 E-12; DG vs CG, p=5.06 E-05), and was more significantly decreased in GG than in DG (p=9.23 E-07). Conclusions Topical administration of dorzolamide colirium diminishes oxidative stress in patients with glaucoma.

scholarly journals Serum Homocysteine and Reactive Species Levels in Fragility Fractures of the Pelvis

2019 ◽  
Vol 70 (9) ◽  
pp. 3216-3219 ◽  
Author(s):  
Alexandru Filip ◽  
Oana Viola Badulescu ◽  
Paul Dan Sirbu ◽  
Elena Cojocaru ◽  
Nina Filip ◽  
...  
Keyword(s):  
Bone Quality ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Fragility Fractures ◽  
Reactive Species ◽  
Control Group ◽  
Serum Homocysteine ◽  
Total Antioxidant ◽  
The Relationship

Although there are studies showing the link between homocysteine and bone quality, the relationship remains controversial. The purpose of the study was to investigate homocysteine levels in patients with fragility fractures of the pelvis and their association with serum Total Antioxidant Status.The study included a group of 60 patients, aged ]65years, with pelvic fragility fractures and osteoporosis admitted in our trauma department between January 2015 and January 2018 and a control group of 30 patients, aged ]65years without fragility fractures or osteoporosis.The total antioxidant status (TAS) and homocysteine (Hcy) concentrations were determined in serum of all the patients. TAS levels had a significantly lower values in the group with pelvic fragility fractures compared with the control group (p[0.001).We noticed a significant higher mean of Hcy levels (p[0.001) in patients with pelvic fragility fractures compared with the control group. As conclusion this study shows that patients with fragility fractures of the pelvis have moderately elevated levels of homocysteine and reduced serum Total Antioxidant Status.

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