scholarly journals Effects of bone morphogenetic proteins on epithelial repair

2021 ◽  
pp. 153537022110281
Author(s):  
Yu Hou ◽  
Yu-Xi He ◽  
Jia-Hao Zhang ◽  
Shu-Rong Wang ◽  
Yan Zhang
Keyword(s):  
Growth Factors ◽  
Epithelial Cells ◽  
Bone Morphogenetic Proteins ◽  
Epithelial Tissue ◽  
Epithelial Repair ◽  
Multiple Functions ◽  
Epithelial Damage ◽  
Damage Repair ◽  
And Migration ◽  
Proliferation And Migration

Epithelial tissue has important functions such as protection, secretion, and sensation. Epithelial damage is involved in various pathological processes. Bone morphogenetic proteins (BMPs) are a class of growth factors with multiple functions. They play important roles in epithelial cells, including in differentiation, proliferation, and migration during the repair of the epithelium. This article reviews the functions and mechanisms of the most profoundly studied BMPs in the process of epithelial damage repair and their clinical significance.

scholarly journals Effect of Slit/Robo signaling on regeneration in lung emphysema

2021 ◽  
Author(s):  
Jin-Soo Park ◽  
RyeonJin Cho ◽  
Eun-Young Kang ◽  
Yeon-Mok Oh
Keyword(s):  
Epithelial Cells ◽  
Mouse Model ◽  
Lung Epithelial Cells ◽  
Mouse Lung ◽  
Chronic Obstructive ◽  
Irreversible Damage ◽  
Lung Epithelial ◽  
And Migration ◽  
Proliferation And Migration

AbstractEmphysema, a pathological component of chronic obstructive pulmonary disease, causes irreversible damage to the lung. Previous studies have shown that Slit plays essential roles in cell proliferation, angiogenesis, and organ development. In this study, we evaluated the effect of Slit2 on the proliferation and migration of mouse lung epithelial cells and its role in regeneration in an emphysema lung mouse model. Here, we have shown that Slit2/Robo signaling contributes to the regeneration of lungs damaged by emphysema. Mouse epithelial lung cells treated with Slit2 exhibited increased proliferation and migration in vitro. Our results also showed that Slit2 administration improved alveolar regeneration in the emphysema mouse model in vivo. Furthermore, Slit2/Robo signaling increased the phosphorylation of ERK and Akt, which was mediated by Ras activity. These Slit2-mediated cellular signaling processes may be involved in the proliferation and migration of mouse lung epithelial cells and are also associated with the potential mechanism of lung regeneration. Our findings suggest that Slit2 administration may be beneficial for alveolar regeneration in lungs damaged by emphysema.

Effects of Long-Chain Non-Coding Ribonucleic Acid Hox Tran Antisense RNA on Proliferation and Migration of Epithelial Cells of Cataract Lens

2021 ◽  
Vol 11 (12) ◽  
pp. 2329-2336
Author(s):  
Qiang Zeng ◽  
Yiting Luo
Keyword(s):  
Epithelial Cells ◽  
Ribonucleic Acid ◽  
Transforming Growth Factor ◽  
Colorimetric Method ◽  
The Other ◽  
Human Lens ◽  
And Migration ◽  
Long Chain ◽  
Proliferation And Migration ◽  
E Cadherin

In order to explore effects of long-chain non-coding ribonucleic acid (RNA) HOTAIR on proliferation and migration of human lens epithelial cells, SRA01/04 cells were selected as the research strain in this study and divided into S1 group (no HOTAIR transfection), S2 group (siHOTAIR transfection), S3 group (siHOTAIR+10 ng/mL TGF-β2), and S4 group (no HOTAIR transfection+10 ng/mL TGF-β2) according to the presence or absence of transforming growth factor (TGF)-β2 and silent HOTAIR treatment. 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide (MTT) colorimetric method was applied to detect cell proliferation.Western blot was used for detection of E-cadherin, zonula occluden-1 (ZO-1), Vimentin, α-smooth muscle actin (SMA), Snail, Slug, zinc finger E-box binding homeobox 1 (ZEB1), and Smad-2 expressions. Results showed that the number of transmembrane cells in S4 group was higher markedly than that of the other groups, but that of S2 group dropped steeply compared with the other groups (P <0.05); E-cadherin (2.59±0.58) and ZO-1 (1.95±0.56) of S2 group increased hugely compared with the other groups, while Vimentin (0.57±0.14) and α-SMA (0.64±0.28) decreased sharply compared with the other groups (P < 0.05); Snail (2.51±0.59), Slug (2.11±0.47), and ZEB1 (2.83±0.53) of S4 group rose obviously compared with the other groups, but the above of S2 group reduced hugely compared with the other groups (P < 0.05); pSmad-2 and pSmad-3 of S4 group elevated greatly compared with the other groups, and those of S2 group reduced hugely compared with the other groups (P < 0.05). In conclusion, HOTAIR high expression could promote TGF-β2-induced SRA01/04 cell proliferation, migration, invasion, and epithelial-mesenchymal trans-differentiation, which was related to TGF-β/Smad signaling pathway.

scholarly journals Effects of enamel matrix proteins on adherence, proliferation and migration of epithelial cells: A real-time in vitro study

2016 ◽  
Vol 13 (1) ◽  
pp. 160-168 ◽  
Author(s):  
Marzena Wyganowska-Swiatkowska ◽  
Paulina Urbaniak ◽  
Daniel Lipinski ◽  
Marlena Szalata ◽  
Karolina Borysiak ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Real Time ◽  
In Vitro Study ◽  
Vitro Study ◽  
Matrix Proteins ◽  
Enamel Matrix ◽  
Enamel Matrix Proteins ◽  
And Migration ◽  
Proliferation And Migration

scholarly journals Haplotypes in BMP4 and FGF Genes Increase the Risk of Peri-Implantitis

2016 ◽  
Vol 27 (4) ◽  
pp. 367-374 ◽  
Author(s):  
Renata Barboza Coelho ◽  
Roberto Gonçalves Junior ◽  
Ricardo de Mello Villas-Boas ◽  
Leticia Ladeira Bonato ◽  
Valquiria Quinelato ◽  
...  
Keyword(s):  
Growth Factors ◽  
Bone Loss ◽  
Bone Morphogenetic Proteins ◽  
Control Group ◽  
Chi Square ◽  
Spontaneous Bleeding ◽  
Osseointegrated Implants ◽  
Polymorphic Genotype ◽  
Chi Square Test ◽  
And Migration

Abstract Despite the success of osseointegrated implants, failures have increased significantly, associated with development of peri-implantitis. Multiple factors influence the peri-implant bone loss, including environmental and genetic causes. BMPs (Bone morphogenetic proteins) are growth factors that induce bone formation. FGF (fibroblast growth factors) and their receptors (FGFRs) play important roles by controlling the levels of cell proliferation, differentiation and migration. BMP/FGF relationship is responsible for promoting bone regeneration and bone loss. The aim of this study was to analyze the correlation between BMP4, FGF3, FGF10 and FGFR1 genes and peri-implant bone loss. Two hundred and fifteen volunteers, with 754 dental implants, were submitted to oral examination and divided in healthy group (n=129) and peri-implantitis group (n=86). Thirteen polymorphisms in BMP4, FGF3, FGF10 and FGFR1 genes were analyzed individually and in haplotype. The chi-square test correlated genotypes, allelic and haplotype frequencies. Values of p<0.05 were considered significant. Volunteers with peri-implantitis demonstrated high incidence of total edentulism (p<0.0001) and thin peri-implant phenotype (p<0.04). Higher incidence of spontaneous bleeding, plaque and implant mobility was observed in peri-implantitis group (p<0.0001 for all). The TT polymorphic genotype for BMP4 rs2761884 was associated with healthy peri-implant (p=0.01). FGF3 rs4631909 (TT+CT genotype) also showed association with the control group (p=0.04). The frequency of C allele for FGF3 rs4631909 showed a tendency for association with peri-implantitis (p=0.08). FGF10 CCTG (p=0.03), BMP4 GAAA (p=0.05) and GGGA (p=0.02) haplotypes were associated with peri-implantitis (p=0.03). Therefore, it may be concluded that BMP4 and FGF10 haplotypes are associated with peri-implantitis.

scholarly journals Long Non-Coding RNA H19 Regulates Human Lens Epithelial Cells Function

2018 ◽  
Vol 50 (1) ◽  
pp. 246-260 ◽  
Author(s):  
Xin Liu ◽  
Chang Liu ◽  
Kun Shan ◽  
Shujie Zhang ◽  
Yi Lu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Epithelial Cells ◽  
Differentially Expressed ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
Double Staining ◽  
Human Lens Epithelial Cells ◽  
And Migration ◽  
Proliferation And Migration

Background/Aims: Age-related cataract (ARC) remains the leading cause of visual impairment among the elderly population. Long non-coding RNAs (lncRNAs) have emerged as potential regulators in many ocular diseases. However, the role of lncRNAs in nuclear ARC, a subtype of ARC, requires further elucidation. Methods: LncRNA sequencing was performed to identify differentially expressed lncRNAs between the capsules of transparent and nuclear ARC lenses. Expression validation was confirmed by qRT-PCR. MTT assay, Calcein-AM and propidium iodide double staining, Rhodamine 123 and Hoechst double staining, EdU and transwell assay were used to determine the role of H19 or miR-675 in the viability, apoptosis, proliferation and migration of primary cultured human lens epithelial cells (HLECs). Bioinformatics and luciferase reporter assays were used to identify the binding target of miR-675. Results: Sixty-three lncRNAs are differentially expressed between the capsules of transparent and nuclear ARC lenses. One top abundantly expressed lncRNA, H19, is significantly up-regulated in the nuclear ARC lens capsules and positively associated with nuclear ARC grade. H19 knockdown accelerates apoptosis development and reduces the proliferation and migration of HLECs upon oxidative stress. H19 is the precursor of miR-675, and a reduction of H19 inhibits miR-675 expression. miR-675 regulates CRYAA expression by targeting the binding site within the 3’UTR. Moreover, miR-675 increases the proliferation and migration while decreasing the apoptosis of HLECs upon oxidative stress. Conclusion: H19 regulates HLECs function through miR-675-mediated CRYAA expression. This finding would provide a novel insight into the pathogenesis of nuclear ARC.

The Effect of Antiamoebic Agents on Viability, Proliferation and Migration of Human Epithelial Cells, Keratocytes and Endothelial Cells, In Vitro

2018 ◽  
Vol 43 (6) ◽  
pp. 725-733 ◽  
Author(s):  
Lei Shi ◽  
Tanja Stachon ◽  
Berthold Seitz ◽  
Stefan Wagenpfeil ◽  
Achim Langenbucher ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Epithelial Cells ◽  
Human Epithelial Cells ◽  
And Migration ◽  
Proliferation And Migration

Polyphosphate, the physiological metabolic fuel for corneal cells: a potential biomaterial for ocular surface repair

2019 ◽  
Vol 7 (12) ◽  
pp. 5506-5515 ◽  
Author(s):  
Xiaohong Wang ◽  
Adrian Gericke ◽  
Maximilian Ackermann ◽  
Shunfeng Wang ◽  
Meik Neufurth ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Ocular Surface ◽  
Inorganic Polymer ◽  
Metabolic Fuel ◽  
Corneal Cells ◽  
And Migration ◽  
Surface Repair ◽  
Proliferation And Migration ◽  
Ocular Surface Repair

Polyphosphate, a natural inorganic polymer that acts as a reservoir for metabolic fuel (ATP), increases the proliferation and migration potency of epithelial cells, covering the avascular cornea.

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