Triterpenoids and Sterols from the Stem of Alnus Formosana Burk

The chemical constituents of the stem of Alnus formosana Burk. were investigated and sixteen known compounds, composed of eleven triterpenoids and five sterols, were isolated and characterized from the n-hexane- and chloroform-soluble fractions of the methanol extract. Of these, seven triterpenoids, lupeol, lupenone, betulinic acid, 3-O-acetylbetulinic acid, 3-O-acetylerythrodiol, 3-O-acetyloleanolic aldehyde, and taraxerone, were isolated for the first time from an Alnus species. The isolation of the five sterols, stigmastanone, stigmast-4-en-3-one, β-sitosterol, β-sitosteryl-β-D-glucoside, and stigmasta-3,6-dione, is also the first time that the presence of such natural products has been recorded for an Alnus species. Taraxerone and betulinic acid were the major non-polar constituents of the stem of A. formosana.

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Chemical Constituents of the Lichen Cladina macaronesica

Zeitschrift für Naturforschung C ◽

10.1515/znc-1991-1-203 ◽

1991 ◽

Vol 46 (1-2) ◽

pp. 12-18 ◽

Cited By ~ 8

Author(s):

Antonio G . González ◽

Jaime Bermejo Barrera ◽

Elsa Ma Rodríguez Pérez ◽

Consuelo E. Hernández Padrón

Keyword(s):

Natural Products ◽

Phenolic Compounds ◽

Silver Nitrate ◽

Silica Gel ◽

Column Chromatography ◽

Chemical Constituents ◽

Usnic Acid ◽

Acetone Extract ◽

First Time

Column chromatography of the acetone extract of the lichen Cladina macaronesica (Sephadex LH-20, silica gel and silver nitrate-im pregnated silica gel) afforded eight triterpenes identified by chemical and spectral m eans. α-Amyrenone, lupenone, taraxerol, taraxerone and /so-arborinol acetate were isolated for the first time from lichens and (-)-usnic acid and five mononuclear phenolic compounds were also obtained, four for the first time as natural products. The possible transformation of perlatolic acid into these phenolic compounds is briefly outlined.

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Chemical Constituents and Protection of Biodiversity of Corypha taliera Roxb., a Critically Endangered Plant of Bangladesh

Bangladesh Pharmaceutical Journal ◽

10.3329/bpj.v20i2.37888 ◽

2018 ◽

Vol 20 (2) ◽

pp. 213-220

Author(s):

Akhtaruzzaman Chowdhury ◽

Md Ashraful Alam ◽

Md Shafiullah Shajib ◽

Mohammad Abdullah Al Mansur ◽

Mohammad A Rashid

Keyword(s):

Spectroscopic Analysis ◽

Methanol Extract ◽

Chemical Constituents ◽

Pharmaceutical Journal ◽

Critically Endangered ◽

Ex Situ ◽

Endangered Plant ◽

Separation And Purification ◽

First Time ◽

Mature Seeds

This article focuses on the chemical constituents and protection of biodiversity through plantation of saplings of Corypha taliera Roxb., a critically endangered plant of Bangladesh. Until 2010, the tree in the campus of University of Dhaka, used to be considered as the lone surviving species in the world in nature. Succesive chromatographic separation and purification of the methanol extract of air dried flowers of C. taliera provided β-sitosterol (1), β-amyrin (2), and betulinic acid (3) for the first time from its flowers. The structures of these purified compounds were established by extensive spectroscopic analysis and comparison of spectral data with published values as well as co-TLC with authentic samples. On the other hand, 500 mature seeds were sown in seed beds in the Medicinal Plant Garden of Faculty of Pharmacy, University of Dhaka, and Azimpur Government Officers' Quarter premises. After 40 days, the root was first seen to grow in its habitat and 85 days later the shoot developed up to 2.5 cm in height. The rate of germination was found to be 89-93%. The produced saplings were later on planted in different places of Bangladesh for conservation of the plant and protection of biodiversity by ex situ arrangement.Bangladesh Pharmaceutical Journal 20(2): 213-220, 2017

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CHEMICAL CONSTITUENTS OF THE ROOT OF STREPTOCAULON JUVENTAS MERR.

Science and Technology Development Journal ◽

10.32508/stdj.v14i2.1940 ◽

2011 ◽

Vol 14 (2) ◽

pp. 28-35

Author(s):

Hao Xuan Bui ◽

Duc Minh Nguyen ◽

Quan Le Tran

Keyword(s):

Methanol Extract ◽

Chemical Constituents ◽

Spectroscopic Methods ◽

First Time

From the methanol extract of the root of Streptocaulon juventas Merr, three cardenolide derivatives were isolated. Their strutures were determined by spectroscopic methods. This is the first time that (17α)-H-periplogenin-3-O-β-D-glucopyranosyl-(1–4)-2-O-acetyl-3-O-methyl-β- fucopyranoside (1) and periplogenin-3-O-β-cymaropyranosyl-(1®4)--glucopyranoside (2) were isolated from the root of S. juventas together with the known 17α-H-periplogenin-3-O-β- digitoxopyranosyl-(1®4)-O-β-glucopyranosyl-(1®6)-O-β-glucopyranoside (3).

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STUDY ON CHEMICAL CONSTITUENTS AND CYTOTOXICITY OF ASPIDISTRA LETREAE

HUE UNIVERSITY JOURNAL OF SCIENCE NATURAL SCIENCE ◽

10.26459/hueuni-jns.v129i2a.5656 ◽

2020 ◽

Vol 129 (1B) ◽

Author(s):

Duc Viet Ho

Keyword(s):

Methanol Extract ◽

Human Cancer ◽

Chemical Constituents ◽

Hep G2 ◽

Pure Compound ◽

Human Cancer Cell Lines ◽

Phytochemical Investigation ◽

Reported Data ◽

First Time

A phytochemical investigation of whole Aspidistra letreae plants led to the isolation of 2H-chromen-2-one (1), α-tocopherol (2), (E)-phytol (3), asparenydiol (4) and (25S)-spirost-1β,3α,5β-triol (5). Their structures were determined on the basis of NMR spectral evidences and in comparison with the reported data. Of these, asparenydiol (4) was isolated from the genus Aspidistra for the first time. This is also the first report on the separation and structural determination of (25S)-spirost-1β,3α,5β-triol (5) as a pure compound. The methanol extract exhibited a moderate cytotoxicity against the LU-1, HeLa, MDA-MB-231, Hep-G2, and MKN-7 human cancer cell lines with IC50 values ranging from 52.58 ± 3.65 to 64.78 ± 4.89 μg/mL.

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Triterpenes from the Mushroom Hypholoma lateritium: Isolation, Structure Determination and Investigation in Bdelloid Rotifer Assays

Molecules ◽

10.3390/molecules24020301 ◽

2019 ◽

Vol 24 (2) ◽

pp. 301 ◽

Cited By ~ 3

Author(s):

Bayar Chuluunbaatar ◽

Zoltán Béni ◽

Miklós Dékány ◽

Bernadett Kovács ◽

András Sárközy ◽

...

Keyword(s):

Natural Products ◽

Structure Determination ◽

Methanol Extract ◽

Bdelloid Rotifer ◽

Spectroscopic Analyses ◽

Experimental Environment ◽

Low Toxicity ◽

First Time ◽

Decadienoic Acid

Twelve compounds (1–12) were isolated from the methanol extract of brick cap mushroom (Hypholoma lateritium (Schaeff.) P. Kumm.). The structures of the compounds were elucidated using extensive spectroscopic analyses, including NMR and MS measurements. Lanosta-7,9(11)-diene-12β,21α-epoxy-2α,3β,24β,25-tetraol (1) and 8-hydroxy-13-oxo-9E,11E-octa-decadienoic acid (2) were identified as new natural products, together with ten known compounds, from which 3β-hydroxyergosta-7,22-diene (4), demethylincisterol A2 (5), cerevisterol (6), 3β-O-glucopyranosyl-5,8-epidioxyergosta-6,22-diene (7), fasciculol E (9), and uridine (12) were identified in this species for the first time. The isolated triterpenes (1, 3–11) were investigated for their toxicity in vivo using bdelloid rotifer assays. Most of the examined steroids in general showed low toxicity, although the effects of the compounds varied in a wider range from the non-toxic lanosta-7,9(11)-diene-12β,21α-epoxy-2α,3β,24β,25-tetraol (1) to the significantly toxic cerevisterol (6), with substantial dependence in some cases on the presence of nutrient in the experimental environment.

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Phytochemical Investigation of Schleichera oleosa (Lour.) Oken Leaf

Bangladesh Pharmaceutical Journal ◽

10.3329/bpj.v24i1.51633 ◽

2021 ◽

Vol 24 (1) ◽

pp. 33-36

Author(s):

Noushin Anjum ◽

Md Jamal Hossain ◽

Mohammad Rashedul Haque ◽

Akhtaruzzaman Chowdhury ◽

Mohammad A Rashid ◽

...

Keyword(s):

Secondary Metabolites ◽

Medicinal Plant ◽

Betulinic Acid ◽

Methanol Extract ◽

Folk Medicine ◽

Pharmaceutical Journal ◽

Nmr Data ◽

Phytochemical Investigation ◽

Schleichera Oleosa ◽

Soluble Fractions

Schleichera oleosa (Lour.) Oken. is a medicinal plant of Bangladesh with enormous traditional applications in folk medicine. The current study was designed to isolate the secondary metabolites by successive chromatographic separation of n-hexane and dichloromethane soluble fractions of a methanol extract of leaves of S. oleosa. A total of four compounds were separated and identified as 5,7-dihydroxy-4'-methoxyflavone, stigmasterol, lupeol and betulinic acid. The structures of the isolated compounds were elucidated by analysis of their 1H NMR data and comparison with published values. Bangladesh Pharmaceutical Journal 24(1): 33-36, 2021

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Chemical Constituents and Their Activities From the Twigs of Euscaphis konishii Hayata

Natural Product Communications ◽

10.1177/1934578x20934933 ◽

2020 ◽

Vol 15 (7) ◽

pp. 1934578X2093493

Author(s):

Jingxin Chen ◽

Lin Ni ◽

Yao Zhang ◽

Yingsa Zhu ◽

Wei Huang ◽

...

Keyword(s):

Cell Line ◽

Ellagic Acid ◽

Betulinic Acid ◽

Hepg2 Cells ◽

Chemical Constituents ◽

Hepatocarcinoma Cell ◽

Human Hepatocarcinoma Cell Line ◽

First Time ◽

Hepatocarcinoma Cell Line ◽

Tormentic Acid

A new ellagic acid derivative 3,3′-di- O-methylellagic acid 4 ′-α-l-arabinopyranoside (1), with 9 known compounds identified as 3,3′-di- O-methylellagic acid (2), 3,3′-di- O-methylellagic acid 4′-α-d-arabinofuranoside (3), 3,3′-di- O-methylellagic acid 4′ -β-d-glucopyranoside (4), 3,3′-di- O-methylellagic acid 4 ′-β-d-xylopyranoglucoside (5), 3,3′,4-tri- O-methylellagic acid 4′-β-d-glucopyranoside (6), tormentic acid (7), ursolic acid (8), euscaphic acid (9), and betulinic acid (10), was isolated from the twigs of Euscaphis konishii Hayata. Compounds 1, 3, and 5 to 7 were isolated from this plant for the first time, and compounds 1 and 5 were obtained from the plant genus for the first time. The structure of the new compound was confirmed by HRESIMS, NMR, and compared with data from the literature . The cytotoxicities of 10 isolated compounds were tested, with compounds 1 to 6 showing moderately inhibited activity against the Human Hepatocarcinoma cell line (HepG2 cells) with an IC50 value ranging from 69.7 to 181.8 μM.

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Chemical constituents from Penianthus camerounensis Dekker (Menispermaceae)

Zeitschrift für Naturforschung B ◽

10.1515/znb-2019-0072 ◽

2019 ◽

Vol 74 (10) ◽

pp. 703-708 ◽

Cited By ~ 1

Author(s):

Georges Bellier Tabekoueng ◽

Carine Mvot Akak ◽

Moses K. Langat ◽

Anatole Guy Blaise Azebaze ◽

Alain François Kamdem Waffo ◽

...

Keyword(s):

Nmr Spectroscopy ◽

Chemical Analysis ◽

Betulinic Acid ◽

Chemical Constituents ◽

2D Nmr ◽

2D Nmr Spectroscopy ◽

Phytochemical Study ◽

Chemical Structures ◽

First Time

AbstractThe phytochemical study of the roots, leaves and twigs of Penianthus camerounensis Dekker (Menispermaceae) has led to the isolation and the characterization of 20 compounds. A ceramide, camerounamide (1), and a furoclerodanediterpenoid, camerounin (2), have not been described previously, while the compounds xylopic acid (3), syringaresinol (4), iso-propylmethylcyclohexa-1,4-diol (5), 1-(28-hydroxyoctacosanoyl)glycerol (6), scoparone (7), friedelin (8), friedelanol (9) and betulinic acid (10) are being reported for the first time from the genus Penianthus alongside 10 known compounds (11–20). Chemical structures were determined using 1D- and 2D-NMR spectroscopy, MS and chemical analysis. Their chemotaxonomic importance is discussed.

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Primary investigating chemical constituents of bioactive extract from Centrostachys aquatica (R.Br.) Wall. ex Moq.-Tand.

Can Tho University Journal of Science ◽

10.22144/ctu.jen.2021.043 ◽

2021 ◽

Vol 13 (3) ◽

pp. 71-78

Author(s):

Quoc Luan Ngo ◽

Thao Cuong Ta ◽

Thi Manh Huynh Tran ◽

Minh Dan Le ◽

Khac Khong Minh Ngo ◽

...

Keyword(s):

Ethyl Acetate ◽

Methanol Extract ◽

Biological Activities ◽

Chemical Constituents ◽

Mekong Delta ◽

Cancer Cell Line ◽

Published Data ◽

Antioxidant Ability ◽

First Time ◽

Crude Methanol Extract

This study is aimed to screen the biological activities and chemical composition to find evidences for potential medicinal applications of Centrostachys aquatica in the Mekong Delta. Crude methanol extract and subextracts in n-hexane, ethyl acetate, and acetone from Centrostachys aquatica were tested bioactivities. The methanol extract, n-hexane and ethyl acetate subextracts exhibited antimicrobial activity with corresponding MIC values of 200, 100 and 200 µg/mL, respectively. The ethyl acetate subextract was inhibited cytotoxicity against cancer cell line LU-1 with IC50 of 27.66 µg/mL. None of the extracts showed antioxidant ability. Three known secondary metabolites including oleanolic acid (1), 20(E)-hydroxy-b-ecdysone (2), and b-spinasterol (3) were isolated for the first time from the bioactive (ethyl acetate) subextract of Centrostachys aquatica. Their structures were elucidated by modern spectra as MS, NMR and comparison with published data.

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Chemical Constituents Isolated from the Leaves of Toricellia angulata Oliv. var. intermedia (Harms.) Hu

Natural Product Communications ◽

10.1177/1934578x211045464 ◽

2021 ◽

Vol 16 (10) ◽

pp. 1934578X2110454

Author(s):

Zhong-Yao Han ◽

Fu-Jun Zhou ◽

Yuanqiang Guo ◽

Zheng-Min Yang ◽

Fang Cao ◽

...

Keyword(s):

Data Analysis ◽

Chemical Composition ◽

Methanol Extract ◽

Chemical Constituents ◽

Folk Medicine ◽

Coumarin Derivative ◽

Iridoid Glycoside ◽

Nmr Data ◽

First Time ◽

Cyclohexanone Derivative

In our survey on the chemical composition of Chinese folk medicines, nine compounds were isolated from methanol extract of the leaves of Toricellia angulata Oliv. var. intermedia (Harms.) Hu (Corniaceae). The structures of these compounds were elucidated on the basis of NMR data analysis, which were identified as dimethyl 2-(hydroxymethyl)-5-oxocyclohexane-1,4-dicarboxylate (1), methyl succinate (2), 5-hydroxymethyl-2-furfuraldehyde (3), 7-hydroxy-6-methoxycoumarin (4), loliolide (5), (8 S)-deca-2-trans-2,9-diene-4,6-diyn-1,8-diol (6), methyl malate (7), griselinoside (8), and methyl linoleate (9), respectively. Among them, compound 1 is a new cyclohexanone derivative and given a trivial name torriangulate A, while others are categorized to be organic acids (2, 7, and 9), a coumarin derivative (4), a terpene lactone (5), a polyacetylene (6), and an iridoid glycoside (8). Compounds 3–5 were isolated from this genus for the first time and compound 9 was first identified from this species. The discovered compounds with novel or known structures further reveal the chemical basis of T. angulata var . intermedia, which lays a foundation for the development of T. angulata var . intermedia used as a traditionally folk medicine.

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