Primary investigating chemical constituents of bioactive extract from Centrostachys aquatica (R.Br.) Wall. ex Moq.-Tand.

This study is aimed to screen the biological activities and chemical composition to find evidences for potential medicinal applications of Centrostachys aquatica in the Mekong Delta. Crude methanol extract and subextracts in n-hexane, ethyl acetate, and acetone from Centrostachys aquatica were tested bioactivities. The methanol extract, n-hexane and ethyl acetate subextracts exhibited antimicrobial activity with corresponding MIC values of 200, 100 and 200 µg/mL, respectively. The ethyl acetate subextract was inhibited cytotoxicity against cancer cell line LU-1 with IC50 of 27.66 µg/mL. None of the extracts showed antioxidant ability. Three known secondary metabolites including oleanolic acid (1), 20(E)-hydroxy-b-ecdysone (2), and b-spinasterol (3) were isolated for the first time from the bioactive (ethyl acetate) subextract of Centrostachys aquatica. Their structures were elucidated by modern spectra as MS, NMR and comparison with published data.

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Studies on Chemical Constituents and Biological Activities of an Endophytic Fungi from Magnifera indica L.

Dhaka University Journal of Pharmaceutical Sciences ◽

10.3329/dujps.v13i1.21862 ◽

2015 ◽

Vol 13 (1) ◽

pp. 63-67

Author(s):

Mozammel Haque ◽

Mohammad Shoeb ◽

Nilufar Nahar

Keyword(s):

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Biological Activities ◽

Chemical Constituents ◽

Radical Scavenging ◽

Acid Methyl Ester ◽

Diffusion Method ◽

Published Data ◽

Nmr Studies ◽

Brine Shrimp Lethality Assay

Two compounds, ergosterol (1) and 4-hydroxy-hexadec-6-enoic acid methyl ester (2) were isolated from the ethyl acetate extract of the endophytic fungal strain labeled as MI-3, isolated from the leave of Magnifera indica L. The structures of the isolated compounds were elucidated by 1H NMR studies and comparing with published data. The crude ethyl acetate extract, three column fractions and ergosterol were tested for antimicrobial activity against five Gram-positive and eight Gram-negative bacteria and three fungi by disc diffusion method. The general toxicity and antioxidant activity of the parent extract, column fractions and ergosterol were also evaluated by using brine shrimp lethality assay and free radical scavenging assay, respectively. Low activities were observed in all cases. DOI: http://dx.doi.org/10.3329/dujps.v13i1.21862 Dhaka Univ. J. Pharm. Sci. 13(1): 63-67, 2014 (June)

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STUDY ON CHEMICAL CONSTITUENTS AND BIOLOGICAL ACTIVITIES OF ALPINIA KWANGSIENSIS T. L. WU AND S. J. CHEN COLLECTED IN VIETNAM

Vietnam Journal of Science and Technology ◽

10.15625/2525-2518/56/4a/12909 ◽

2018 ◽

Vol 56 (4A) ◽

pp. 273

Author(s):

Tran Thi Minh

Keyword(s):

Staphylococcus Aureus ◽

Antioxidant Activity ◽

Bacillus Subtilis ◽

Ethyl Acetate ◽

Spectroscopic Data ◽

Methanol Extract ◽

Biological Activities ◽

Chemical Constituents ◽

Ethyl Acetate Fraction ◽

Ic50 Values

Three compounds named ester methyl-trans-p-coumarate (1), 7-hydroxy-6-methoxycoumarin (2), and (+)-gallocatechin (3) have been isolated from the ethyl acetate fraction of methanol extract of Alpinia kwangsiensis roots collected in Thai Nguyen province. Their structures were elucidated on the basis of spectroscopic data and by comparison with their spectral data reported in literature. This is the first isolation of three compounds from this species. The ethyl acetate fraction was found to be active against bacterias as Staphylococcus aureus and Bacillus subtilis with the IC50 values ranging at 74.65 mg/ml and 80.54 mg/ml, respectively. This fraction also showed antioxidant activity through DPPH test with the EC50 value 87.98 mg/ml.

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Chemical constituents of the whole plant of PHLOGACANTHUS TURGIDUS (FUA EX HOOK.F.) LINDAU

Science and Technology Development Journal - Natural Sciences ◽

10.32508/stdjns.v5i3.1071 ◽

2021 ◽

Vol 5 (3) ◽

pp. first

Author(s):

Nguyen Tan Phat ◽

Mai Thanh Chi ◽

Phan Nhat Minh ◽

Dang Chi Hien ◽

Mai Dinh Tri

Keyword(s):

Biological Activities ◽

Chemical Constituents ◽

Ethanolic Extract ◽

2D Nmr ◽

Published Data ◽

Cytotoxic Activities ◽

Phenylethanoid Glycosides ◽

Whole Plant ◽

The Family ◽

First Time

The genus Phlogacanthus belongs to the family Acanthaceae and consists of more than 49 species, These species are widespread in tropical and subtropical zones such as Bangladesh, Bhutan, China, Indonesia, India, Myanmar and Vietnam. In Vietnam, the genus contains about 06 species. The extracts from some species in this genus have been evaluated for biological activities as analgesic, antiinflammatory, antimicrobial, antioxidant, antihyperglycemic and cytotoxic activities. In this study, the whole plant of Phlogacanthus turgidus was collected, dried, cut into small species, and extracted with ethanol to yield the ethanolic extract. Isolation of chemical constituents was performed using column chromatography on silica gel; their structures were elucidated by HRESI-MS, 1D & 2D-NMR and compared with published data. Six compounds, including two nor-isoprenoids: (+)-dehydrovomifoliol (1), (3S,5R,6R,7E,9S)-megastiman-7-ene-3,5,6,9-tetrol (2), two steroids b - sitosterol (3), daucosterol (4), one lignan (+)-syringaresinol (5), a derivative of phenylethanoid glycosides: martynoside (6) were isolated from the ethyl acetate extract of the whole plant of P. turgidus. Among them, 1, 2 were reported for the first time from the genus Phlogacanthus, while 3, 4, 5, 6, were found for the first time from this species.

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A study on Antioxidant Properties of Different Extracts from Kitaibelia balansae

Proceedings ◽

10.3390/proceedings2019040023 ◽

2019 ◽

Vol 40 (1) ◽

pp. 23

Author(s):

Zengin ◽

Aygun ◽

Aktumsek

Keyword(s):

Ethyl Acetate ◽

Human Body ◽

Antioxidant Properties ◽

Water Extract ◽

Antioxidant Ability ◽

Chemical Methods ◽

Potential Source ◽

First Time ◽

Antioxidant Effects ◽

Ethyl Acetate Extracts

Nowadays, knowledge of ancient botanical medicinal practices and application of modern phytochemical techniques have provided the excellent tools for the purification and structural elucidation of various phyto-compounds, which, in turn, has given insights into their mode of action on the human body. This study has been designed to investigate for the first time the antioxidant effects of the ethyl acetate, methanolic, and water extracts of Kitaibelia balansae. Different chemical methods were performed and the observed abilities depend on the solvent used. The best antioxidant ability was noted in water extract, followed by methanol and ethyl acetate extracts. The highest level of phenolic was also detected in water extract. The present findings suggest that K. balansae can be considered as a potential source of bioactive compounds for novel phytopharmaceuticals development

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Chemical Constituents of Eupatorium japonicum and Anti-Inflammatory, Cytotoxic, and Apoptotic Activities of Eupatoriopicrin on Cancer Stem Cells

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2021/6610347 ◽

2021 ◽

Vol 2021 ◽

pp. 1-12

Author(s):

Minh Giang Phan ◽

Thi Thao Do ◽

Thi Nga Nguyen ◽

Thi Viet Huong Do ◽

Ngoc Phuc Dong ◽

...

Keyword(s):

Anticancer Agents ◽

Human Cancer ◽

Biological Activities ◽

Chemical Constituents ◽

Sesquiterpene Lactones ◽

No Production ◽

Bioactive Constituents ◽

Anti Inflammatory ◽

First Time ◽

Eupatorium Japonicum

Eupatorium japonicum Thunb. of the plant family Asteraceae is a popular traditional herb in Vietnam. However, its chemical constituents as well as bioactive principles have not been investigated yet. We investigated the phytochemistry of E. japonicum in Vietnam and isolated seventeen compounds (1–17) including phytosterols, terpenoids, phenolic acids, flavonoids, fatty alcohols, and fatty acids. They were structurally determined by MS and NMR analysis. Except for compounds 6 and 12, all the other compounds were identified for the first time from E. japonicum. Since many sesquiterpene lactones with α-methylene γ-lactone ring are reported as anti-inflammatory and anticancer agents, eupatoriopicrin (10), 1-hydroxy-8-(4,5-dihydroxytigloyloxy)eudesma-4(15),11(13)-dien-6,12-olide (11) were selected among the isolates for biological assays. Compound 10 was identified as the main bioactive sesquiterpene lactone of E. japonicum showing its potent anti-inflammatory and cytotoxic activity through inhibiting NO production and the growth of HepG2 and MCF-7 human cancer cell lines. For the first time, eupatoriopicrin (10) was demonstrated to strongly inhibit NTERA-2 human cancer stem cell (CSC) line in vitro. It is noticeable that the cytotoxicity of eupatoriopicrin against NTERA-2 cells is mediated by its apoptosis-inducing capability of 10 as demonstrated by the results of Hoechst 33342 staining, flow cytometry apoptosis analysis, and caspase-3 activity assays. The biological activities of the main bioactive constituents 1–7, 10, 12, and 15 supported the reported anti-inflammatory and anticancer properties of extracts from E. japonicum.

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CONTRIBUTE TO STUDY ON CHEMICAL CONSTITUENTS OF URENA LOBATA (L.) OF VIETNAM

Vietnam Journal of Science and Technology ◽

10.15625/2525-2518/57/2/13019 ◽

2019 ◽

Vol 57 (2) ◽

pp. 162

Author(s):

Quan Minh Pham ◽

Hoai Van Thi Tran ◽

Lam Tien Do ◽

Phuong Lan Doan ◽

Inh Thi Cam ◽

...

Keyword(s):

Mental Disorders ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Chemical Constituents ◽

2D Nmr ◽

Spectroscopic Methods ◽

Tree Roots ◽

Chemical Structures ◽

Phytochemical Investigation ◽

First Time

Urena lobata L. is used in Vietnamese traditional medicine for the treatment of several diseases. Tree roots are used to treat rheumatism, dysentery, poor digestion, flu, tonsils, malaria, asthma, goiter. Flowers are used to treat chickenpox, fever, and mental disorders. Branches, leaves or whole trees used to treat injuries bruises, rheumatism, mastitis, bites. Phytochemical investigation of the n-hexan and ethyl acetate extract of Urena lobata L. led to the isolation of β-sitosterol (1), β-sitosterol-3-O-β-D-glucopyranoside (2), a-acetylamino-phenylpropyl a-benzoylamino-phenylpropanoate (3), quercetin (4), and trans-tiliroside (5). Their chemical structures were determined by spectroscopic methods including MS, 1D, 2D NMR and comparing with those reported in previous papers. Two compounds 3, 5 were isolated for the first time from Urena lobata plant.

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In Vitro Screening for Cytotoxic, Anti-bacterial, Anti-HIV1-RT Activities and Chemical Constituents of Croton fluviatilis, Croton acutifolius, and Croton thorelii

The Natural Products Journal ◽

10.2174/2210315511666210119125611 ◽

2021 ◽

Vol 11 ◽

Author(s):

Charina Worarat ◽

Wilart Pompimon ◽

Phansuang Udomputtimekakul ◽

Sukee Sukdee ◽

Punchavee Sombutsiri ◽

...

Keyword(s):

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Biological Activities ◽

Chemical Constituents ◽

Bacterial Activity ◽

Cytotoxic Activities ◽

Separation And Purification ◽

Crude Extracts ◽

Cytotoxicity Activities

Background: Although the chemical constituents and biological activities of a large number of plants in the Croton genus have been studied, there are still recently discovered plants to be investigated. Objective: 1. To investigate the anti-bacterial, anti-HIV1-RT, and cytotoxicity activities of crude extracts from these plants. 2. To investigate the chemical constituents of Croton fluviatilis, Croton acutifolius, and Croton thorelii. Method: The anti-bacterial, anti-HIV1-RT, and cytotoxicity of the three plants were evaluated by standard techniques. Extraction, separation, and purification of extracts from the three plants were undertaken. Results: The ethyl acetate extract of C. fluviatilis showed low anti-bacterial activity against E. aerogenes, E. coli 0157: H7, and P. mirabilis, together with the ethyl acetate extract of C. acutifolius displayed low anti-bacterial activity against E. aerogenes, while all the crude extracts of C. thorelii were inactive. The ethyl acetate extracts of C. thorelii, and C. fluviatilis showed strong inhibited HIV1-RT, whereas the ethyl acetate extract of C. acutifolius, and the hexane extract of C. fluviatilis displayed moderate inhibited HIV1-RT. Cytotoxic properties of three Croton plants were specific to KKU-M213, MDA-MB-231, A-549, and MMNK-1. Especially, the ethyl acetate extract of C. acutifolius exhibited strong cytotoxic activities against MDA-MB-231, A-549, and MMNK-1. Furthermore, the ethyl acetate extract of C. thorelii showed high cytotoxic activities against KKU-M213, and MDA-MB-231. Compounds 1, and 4 were found in C. fluviatilis. Compounds 2 and 4 were also found in C. acutifolius. Moreover, compound 3 was only found in C. thorelii. Conclusion: The present study revealed that the three Croton species are good sources of flavonoid compounds and further investigation of the chemical constituents from these plants may prove to be fruitful to discover more active compounds to be tested as potential medicines.

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Chemical Constituents from Farfugium Japonicum Var. formosanum

Natural Product Communications ◽

10.1177/1934578x1200700405 ◽

2012 ◽

Vol 7 (4) ◽

pp. 1934578X1200700 ◽

Cited By ~ 1

Author(s):

Sung-Fei Hsieh ◽

Tain-Jye Hsieh ◽

Mohamed El-Shazly ◽

Ying-Chi Du ◽

Chin-Chung Wu ◽

...

Keyword(s):

Chemical Constituents ◽

Cancer Cell Line ◽

Inflammatory Activity ◽

Human Neutrophils ◽

Spectral Techniques ◽

Methanolic Extracts ◽

Aerial Parts ◽

Anti Inflammatory ◽

Farfugium Japonicum ◽

First Time

Two new eremophilenolides, 6β8β10β-trihydroxyeremophil-7(11)-en-12,8-olide (1) and 3βacetoxy-8α-hydroxy-6β-methoxyeremophila-7(11),9-dien-12,8-olide (2) along with twenty-nine other compounds were obtained from the methanolic extracts of the aerial parts and rhizomes of Farfugium japonicum (L.) Kitam. v ar. formosanum (Hayata) Kitam. (Compositae = Asteraceae). The structures of the isolated compounds were characterized and identified by spectral techniques. Compounds 5, 6, 10, 12-24, 29, and 30, were reported for the first time from this genus. Cytotoxicity and anti-inflammatory activity of the isolated compounds were evaluated. Compounds 3 and 16 possessed moderate cytotoxicity against human breast cancer cell line (MCF 7). Compounds 3, 16, 25 and 26 exhibited moderate cytotoxicity against hepatoma cells (Hep G2 and Hep 3B). With respect to the anti-inflammatory activity, compounds 15 and 16 (each 10 μg/mL) inhibited superoxide anion generation by human neutrophils in response to fMLP/CB by 92.0% and 87.3%, respectively.

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Chemical Constituents and Antioxidant and Biological Activities of the Essential Oil from Leaves of Solanum spirale

Natural Product Communications ◽

10.1177/1934578x1200700740 ◽

2012 ◽

Vol 7 (7) ◽

pp. 1934578X1200700 ◽

Cited By ~ 8

Author(s):

Sukanya Keawsa-ard ◽

Boonsom Liawruangrath ◽

Saisunee Liawruangrath ◽

Aphiwat Teerawutgulrag ◽

Stephen G. Pyne

Keyword(s):

Antioxidant Activity ◽

Essential Oil ◽

Biological Activities ◽

Chemical Constituents ◽

Radical Scavenging ◽

Octadecanoic Acid ◽

Small Cell Lung ◽

Hexahydrofarnesyl Acetone ◽

First Time ◽

Mcf 7

The essential oil of the leaves Solanum spirale Roxb. was isolated by hydrodistillation and analyzed for the first time using GC and GC-MS. Thirty-nine constituents were identified, constituting 73.36% of the total chromatographical oil components. ( E)-Phytol (48.10%), n-hexadecanoic acid (7.34%), β-selinene (3.67%), α-selinene (2.74%), octadecanoic acid (2.12%) and hexahydrofarnesyl acetone (2.00%) were the major components of this oil. The antioxidant activity of the essential oil was evaluated by using the DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging assay. The oil exhibited week antioxidant activity with an IC50 of 41.89 mg/mL. The essential oil showed significant antibacterial activity against both Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus with MIC values of 43.0 μg/mL and 21.5 μg/mL, respectively. It also showed significant cytotoxicity against KB (oral cancer), MCF-7 (breast cancer) and NCI-H187 (small cell lung cancer) with the IC50 values of 26.42, 19.69, and 24.02 μg/mL, respectively.

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SENYAWA ANTIMAKAN TERHADAP Epilachna Sparsa DARI BATANG GAYAM (Inocarpus fagiferus Fosb.)

Jurnal Kimia ◽

10.24843/jchem.2020.v14.i01.p09 ◽

2020 ◽

pp. 51

Author(s):

S. R. Santi ◽

I M. Sukadana ◽

N. N. T. Hanayanthi

Keyword(s):

Ethyl Acetate ◽

Mass Spectra ◽

Methanol Extract ◽

Leaf Tissue ◽

Hexane Extract ◽

Antifeedant Activity ◽

Insecticide Activity ◽

Hexane Extracts ◽

Bottom Side ◽

Crude Methanol Extract

Epilachna sparsa is one of the plant pests that damage the epidermal layer on the bottom side of the leaves, so it can lead to damage of leaf tissue and only the skeleton remains. Gayam (Inocarpus fagiferus) is one of the species of the Legumineceae family which has insecticide activity. This study aims to isolate and identify the antifeedant triterpenoid compound from stem of gayam (Inocarpus fagiferus Fosb.) against Epilachna sparsa larvae. Dried powder of 1200 g gayam stems produced 17.82 g of crude methanol extract with antifeedant activity of 60.74% at a concentration of 5% (w/v) which was further separated by partition to obtain n-hexane, ethyl acetate, and water extracts. Extracts of n-hexane, ethyl acetate, and water at 5% (w/v) concentration showed antifeedant activity of 46.74%, 18.09%, and 8.31% respectively. Futhermore, the most active n-hexane extract was identified by phytochemical tests and LC-MS/MS. The phytochemical test showed n-hexane extract contains triterpenoid compounds. The mass spectra from LC-MS/MS chromatogram peaks with MassLynx V4.1 software and Chemspider database for n-hexane extracts which were previously purified by SPE (Solid method) Phase Extraction) using methanol and dichloromethane (DCM) eluents show the presence of components of flavonol pyrogallol, sucrose2,3,3 ', 4', 6-pentaacetate, soyasaponin I, and a compound with a molecular weight of 685,225 g/mol which cannot be identified further because there is no match spectra with the database. Keywords: antifeedant, Epilachna sparsa, gayam, Inocarpus fagiferus Fosb., triterpenoid.

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