Composition and Antibacterial Activity of Essential Oils of Artemisia Fragrans Willd. Leaves and Roots from Iran

2009 ◽  
Vol 4 (2) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Ali Shafaghat ◽  
Yavar Noormohammadi ◽  
Mohammad Zaifizadeh
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Leaf Oil ◽  
Agar Diffusion Method ◽  
Leaves And Roots ◽  
Main Components ◽  
Total Oil

Hydrodistillation of leaves and roots of Artemisia fragrans Willd. (Asteraceae) yielded 0.9% and 0.1 %(v/w) essential oils, respectively. GC/MS analysis allowed identification of 19 components, which made up 91.1% of the total oil from the leaves, while only 9 compounds (93.8%) were identified in the roots. The main components of the leaf oil were chrysanthenon (23.8%), 1, 8-cineole (23.7%), β-caryophyllene (9.6%), p-cymene (7. 7%), filifolide-A (5.7%) and filifolone (5.7%). In the root oil, the main constituents were camphor (67.0%) and camphene (16.9%). Antibacterial activity was tested against Grampositive and Gram-negative bacteria using the agar diffusion method. Activity was observed against two Gram-positive and one Gram- negative bacteria.

scholarly journals Composition and Antibacterial Screening of the Essential Oils of Leaves and Roots of Espeletiopsis Angustifolia Cuatrec

2007 ◽  
Vol 2 (12) ◽  
pp. 1934578X0700201
Author(s):  
Gina Meccia ◽  
Luis B. Rojas ◽  
Judith Velasco ◽  
Tulia Díaz ◽  
Alfredo Usubillaga
Keyword(s):  
Essential Oils ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Antibacterial Screening ◽  
Staphyloccocus Aureus ◽  
Agar Diffusion Method ◽  
Leaves And Roots ◽  
Total Oil

Hydrodistillation of leaves and roots of Espeletiopsis angustifolia Cuatrec. (Asteraceae) yielded 0.18% and 0.15% essential oils, respectively. GC-MS analysis allowed identification of 24 components, which made up 92.9% of the total oil from the leaves, while only 16 compounds (67.2%) were identified in the roots. The most abundant compounds in the leaves were α-pinene (29.9%), β-caryophyllene (14.1%), α-gurjunene (9.9%), β-pinene (9.6%), and 19-oxo-ent-kaur-16-ene (5.3%). In the roots, the main ones were α-pinene (27.9%), β-pinene (10.9%), β-caryophyllene (10.2%), and bicyclogermacrene (8.6%). Antibacterial activity was tested against Gram-positive and Gram-negative bacteria using the agar diffusion method. Activity was observed only against Gram-positive bacteria. MIC values were determined for Staphyloccocus aureus ATCC 25923 (1000 μg/mL, both roots and leaves) and Enteroccocus faecalis ATCC 29212 (240 μg/mL, roots and 360 μg/ mL, leaves).

scholarly journals Composition of the Essential Oils and Antibacterial Activities of Hymenocrater yazdianus, Stachys obtusicrena and Nepeta asterotricha Three Labiatae Herbs Growing Wild in Iran

2012 ◽  
Vol 7 (1) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Shiva Masoudi ◽  
Abdolhossein Rustaiyan ◽  
Razieh Mohebat ◽  
Mohammad Hossein Mosslemin
Keyword(s):  
Essential Oils ◽  
Antibacterial Activities ◽  
Gram Negative Bacteria ◽  
Inhibitory Zone ◽  
Growth Inhibitory ◽  
Germacrene D ◽  
Leaf Oil ◽  
Main Components ◽  
Total Oil ◽  
Stem Leaf

Water-distilled essential oils from leaves of Hymenocrater yazdianus Rech.f., flowers of Stachys obtusicrena Boiss., and stems and flowers of Nepeta asterotricha Rech.f, which are endemic to Iran, were analyzed by GC and GC/MS. Fifty-five components of the leaf oil of H. yazdianus were characterized, representing 95.1% of the total components detected. The major constituents were identified as 1,8-cineole (17.6%), β-caryophyllene (13.9%), α-pinene (10.6%) and caryophyllene oxide (10.4%). Germacrene-D (37.5%) and α-bisabolol (23.5%) were the main components among the twenty constituents characterized in the flower oil of S. obtusicrena, representing 90.8% of the total components detected. Thirty-five compounds representing 93.0% of the stem oil of N. asterotrica were identified among which terpinen-4-ol (22.8%) and γ-terpinene (14.1%) were the major ones. The flower oil of the species was characterized by higher amounts of terpinen-4-ol (24.8%), 4aα, 7aβ-nepetalactone (18.2%) and 1,8-cineole (11.6%) among the thirty-three components comprising 98.5% of the total oil detected. The antibacterial activity of the stem, leaf and flower oils of Hymenocrater yazdianus, Stachys obtusicrena and Nepeta asterotricha against seven Gram-positive and Gram-negative bacteria were determined using the MIC method. The growth inhibitory zone (mm) was also measured.

ANTIBACTERIAL ACTIVITY OF BARK EXTRACTS OF FICUS GLOMERATA ROXB AGAINST SOME GRAM POSITIVE AND GRAM NEGATIVE BACTERIA

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (05) ◽  
pp. 44-47
Author(s):  
S. D. Bhinge ◽  
◽  
M. G Hogade ◽  
A. S Savali ◽  
H. R. Chitapurkar ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Aqueous Extracts ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Agar Diffusion Method ◽  
Ficus Glomerata ◽  
And Staphylococcus Aureus

Petroleum ether, ethanol and aqueous extracts of the bark of Ficus glomerata (Urticaceae) were screened for their antibacterial activity using agar diffusion method. They were tested against six bacteria; three Gram-positive bacteria (Bacillus subtilis, Bacillus aureus and Staphylococcus aureus) and three Gram-negative bacteria (Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa). The susceptibility of the microorganisms to the extracts of these plants was compared with each other and with selected antibiotics.

scholarly journals Antimicrobial Activity and Chemical Constituents of the Essential Oils from Flower, Leaf and Stem of Gypsophila bicolor from Iran

2011 ◽  
Vol 6 (2) ◽  
pp. 1934578X1100600
Author(s):  
Ali Shafaghat ◽  
Masoud Shafaghatlonbar
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Chemical Constituents ◽  
Substantial Effect ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Germacrene D ◽  
Leaf Oil ◽  
Moderate Effect ◽  
Main Components

The volatile constituents from flower, leaf and stem of Gypsophila bicolor growing in Iran were obtained by hydrodistillation and analyzed by GC and GC/MS. The flower oil was characterized by high amounts of germacrene-D (21.2%), p-cymene (20.6%), bicyclogermacrene (17.6%), γ-dodecadienolactone (13.7%) and terpinolene (9.4%). Twenty-four constituents representing 97.4% of the leaf oil were identified of which germacrene-D (23.4%), terpinolene (14.5%), bicyclogermacrene (7.5%), γ-dodecadienolactone (6.8%), p-cymene (6.7%) and cis-β-ocimene (6.3%) were major components. The main components of the stem oil were γ-dodecadienolactone (28.5%), bicyclogermacrene (14.8%), germacrene-D (12.6%), p-cymene (12.5%), terpinolene (11.6%) and trans-β-ocimene (4.2%). The antimicrobial effects of flower, leaf and stem essential oils from Gypsophila bicolor were studied according to the agar diffusion cup method. The essential oils had a moderate effect on the Gram-positive and Gram-negative bacteria, but had a substantial effect on the fungi studied.

scholarly journals Constituents and Antimicrobial Activity of the Essential Oils from Flower, Leaf and Stem of Helichrysum armenium

2012 ◽  
Vol 7 (5) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Khodam-Ali Oji ◽  
Ali Shafaghat
Keyword(s):  
Essential Oils ◽  
Chemical Constituents ◽  
Antimicrobial Effect ◽  
Bornyl Acetate ◽  
Gram Negative Bacteria ◽  
Caryophyllene Oxide ◽  
Gram Negative ◽  
Leaf Oil ◽  
Moderate Effect ◽  
Main Components

The chemical constituents from the flower, leaf and stem of Helichrysum armenium DC. (Asteraceae) growing in Iran were obtained by hydrodistillation and analyzed by GC and GC/MS. The oil of flower was characterized by higher amount of limonene (21.2%), α-cadinol (18.2%), borneol (11.9%), δ-cadinene (9.0%), bornyl acetate (8.0%) and α-humulene (7.3%). Twenty one constituents representing 96.2% of the chromatographical leaf oil were identified of which limonene (29.2%), α- pinene (14.4%), caryophyllene oxide (6.5%), α-gurjunene (6.3%), bornyl acetate (5.5%) and torreyol (5.2%) were the major components. The main components of the stem oil were limonene (23.6%), α-pinene (13.4%), spathulenol (6.4%), α-gurjunene (6.3%), caryophyllene oxide (5.3%), bornyl acetate (5.2%), β-cubebene (4.8%) and δ-cadinene (4.3%). The composition of the oils is different, although the most abundant components are identical in leaf oil (96.2%). The antimicrobial effect of flower, leaf and stem essential oils from Helichrysum armenium was studied according to the agar diffusion cup method. The essential oils had a moderate effect on the Gram-positive and Gram-negative bacteria and had a substantial fungicidal effect on the fungi under study.

In-vitro Antioxidant and Antibacterial Activity of Methanolic Extract of Shorea robusta Gaertn. F. Resin

2016 ◽  
Vol 6 (4) ◽  
pp. 68
Author(s):  
Sushma Vashisht ◽  
Manish Pal Singh ◽  
Viney Chawla
Keyword(s):  
Antibacterial Activity ◽  
Methanolic Extract ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Gram Positive ◽  
Gram Negative ◽  
Shorea Robusta ◽  
Dose Dependent ◽  
Resin Extract

The methanolic extract of the resin of Shorea robusta was subjected to investigate its antioxidant and antibacterial properties its utility in free radical mediated diseases including diabetic, cardiovascular, cancer etc. The methanol extract of the resin was tested for antioxidant activity using scavenging activity of DPPH (1,1-diphenyl-2-picrylhydrazil) radical method, reducing power by FeCl3 and antibacterial activity against gram positive and gram negative bacteria using disc diffusion method. The phytochemical screening considered the presence of triterpenoids, tannins and flavoniods. Overall, the plant extract is a source of natural antioxidants which might be helpful in preventing the progress of various oxidative stress mediated diseases including aging. The half inhibition concentration (IC50) of resin extract of Shorea robusta and ascorbic acid were 35.60 µg/ml and 31.91 µg/ml respectively. The resin extract exhibit a significant dose dependent inhibition of DPPH activity. Antibacterial activity was observed against gram positive and gram negative bacteria in dose dependent manner.Key Words: Shorea robusta, antioxidant, antibacterial, Disc-diffusion, DPPH.

Antibacterial activity of magnesium oxide nanoparticles prepared by Calcination method

2019 ◽  
Vol 10 (03) ◽  
Author(s):  
Elaf Ayad Kadhem ◽  
Miaad Hamzah Zghair ◽  
Sarah , Hussam H. Tizkam, Shoeb Alahmad Salih Mahdi ◽  
Hussam H. Tizkam ◽  
Shoeb Alahmad
Keyword(s):  
Antibacterial Activity ◽  
Magnesium Oxide ◽  
Diffusion Method ◽  
Oxide Nanoparticles ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Magnesium Oxide Nanoparticles ◽  
Agar Disc

magnesium oxide nanoparticles (MgO NPs) were prepared by simple wet chemical method using different calcination temperatures. The prepared NPs were characterized by Electrostatic Discharge (ESD), Scanning Electron Microscope (SEM) and X-ray Diffraction (XRD). It demonstrates sharp intensive peak with the increase of crystallinty and increase of the size with varying morphologies with respect to increase of calcination temperature. Antibacterial studies were done on gram negative bacteria (E.coli) and gram positive bacteria (S.aureus) by agar disc diffusion method. The zones of inhibitions were found larger for gram positive bacteria than gram negative bacteria, this mean, antibacterial MgO NPs activity more active on gram positive bacteria than gram negative bacteria because of the structural differences. It was found that antibacterial activity of MgO NPs was found it has directly proportional with their concentration.

scholarly journals Antibacterial Activity and Flavonoids Content of Artemisia cina Berg. ex Poljakov Ethyl Acetate Extracts

2021 ◽  
Vol 13 (1) ◽  
pp. 106-112
Author(s):  
Sri Kasmiyati ◽  
Elizabeth Betty Elok Kristiani ◽  
Maria Marina Herawati ◽  
Andreas Binar Aji Sukmana
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Biological Activities ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Flavonoid Content ◽  
Gram Positive ◽  
Gram Negative ◽  
Significant Difference

The medicinal plant-derived bioactive compounds have a potential for many biological activities, including antimicrobial activity. Artemisia cina is a medicinal plant from the Compositae family with the potential of having antitumor, antifungal, and antibacterial activity. This study aimed to determine the antibacterial activity and the flavonoid content of A. Cina’s ethyl acetate extract. Plants samples were extracted by ethyl acetate maceration method. Antibacterial activity was tested against Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus) by a disk diffusion method using 25, 50, and 100 mg/l extract concentrations. The flavonoid contents (quercetin and kaempferol) were measured using High-Performance Liquid Chromatography. The extracts of diploid and polyploid A. cina displayed some antibacterial activity, with the Gram-negative bacteria being more resistant than the Gram-positive counterpart. However, no significant difference was observed between the diploid and polyploid extracts. As for the flavonoid content, the highest quercetin content (0.5501 mg/ml) was found in the polyploid A. cina (J), while the highest kaempferol content (0.5818 mg/ml) was observed in the diploid A. cina (KJT). Although A. cina is widely grown in Indonesia, compared to other Artemisia species, A. cina has not been widely studied, especially its antibacterial  potential and in related to its flavonoid content and the use of ethyl acetate as the extraction solvent.  This study reveals the potential of A. cina as a natural antibacterial agent. 

scholarly journals Chemical Composition and Antibacterial Activity of the Essential Oil of Espeletia nana

2012 ◽  
Vol 7 (5) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Alexis Peña ◽  
Luis Rojas ◽  
Rosa Aparicio ◽  
Libia Alarcón ◽  
José Gregorio Baptista ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Disk Diffusion Method ◽  
Gram Negative ◽  
Gram Positive Bacteria

The essential oil of the leaves of Espeletia nana Cuatrec, obtained by hydrodistillation, was analyzed by GC-MS, which allowed the identification of 24 components, which made up 99.9% of the oil. The most abundant compounds were α-pinene (38.1%), β-pinene (17.2%), myrcene (15.0%), spathulenol (4.2%), bicyclogermacrene (4.0%), α-zingiberene (4.0%), and γhimachalene (3.7%). Antibacterial activity was tested against Gram-positive and Gram-negative bacteria using the agar disk diffusion method. Activity was observed only against Gram-positive bacteria. MIC values were determined for Staphylococcus aureus ATCC 25923(200 μg/mL) and Enterococcus faecalis ATCC 29212 (600 μg/mL).

scholarly journals Composition and Antibacterial Activity of Essential Oils from Leaf, Stem and Root of Chrysanthemum parthenium (L.) Bernh. from Iran

2009 ◽  
Vol 4 (6) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Ali Shafaghat ◽  
Hajar Sadeghi ◽  
Khodamali Oji
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oils ◽  
Salmonella Typhi ◽  
Inhibitory Effects ◽  
Germacrene D ◽  
Leaf Oil ◽  
Main Components ◽  
Microdilution Broth

The water distilled essential oils from leaves, stems and roots of Chrysanthemum parthenium (L.) Bernh. were analyzed by GC and GC/MS methods. The leaf oil was characterized by a high amount of camphor (56.4%), whereas in the stem oil, camphor (26.0%), trans-β-ocimene (23.6%) and germacrene-d (15.0%) were the major constituents. The main components of the root oil were α-pinene (50.0%), trans-β-farnesene (13.8%) and bicyclogermacrene (11.0%). Antibacterial activity of the leaf, stem and root oil were evaluated using the microdilution broth method. The oils showed inhibitory effects on Escherichia coli and Salmonella typhi, but were not active against Staphylococcus aureus.

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