scholarly journals Composition and Antimicrobial Activity of Seseli montanum subsp. tommasinii Essential Oil

2011 ◽  
Vol 6 (2) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Jovana Šiljegović ◽  
Jasmina Glamočlija ◽  
Marina Soković ◽  
Ivan Vucković ◽  
Vele Tešević ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Antimicrobial Effect ◽  
Fungal Species ◽  
Food Contaminants ◽  
Animal Pathogens ◽  
Germacrene D ◽  
Spoilage Fungi ◽  
Strong Antimicrobial Activity

The essential oil isolated from Seseli montanum L. subsp. tommasinii Reich. f. was analyzed by GC/MS and the most abundant components were β-pinene (30.2%), germacrene D (10.1%), sabinene (8.0%), α-pinene (7.2%) and limonene (6.6%). The antimicrobial effect of the essential oil was evaluated against four bacterial and four fungal species among which were food contaminants, spoilage fungi, and plant, fungal and animal pathogens. The oil possessed moderate to strong antimicrobial activity.

scholarly journals Chemical composition and antifungal activity of Salvia sclarea (Lamiaceae) essential oil

2008 ◽  
Vol 60 (2) ◽  
pp. 233-237 ◽  
Author(s):  
Ana Dzamic ◽  
Marina Sokovic ◽  
M. Ristic ◽  
Slavica Grujic-Jovanovic ◽  
Jelena Vukojevic ◽  
...  
Keyword(s):  
Essential Oil ◽  
Fusarium Species ◽  
Trichoderma Viride ◽  
Food Poisoning ◽  
Fungal Species ◽  
Minimal Inhibitory Concentrations ◽  
Microdilution Method ◽  
Salvia Sclarea ◽  
Animal Pathogens ◽  
Spoilage Fungi

Clary sage (Salvia sclarea L.) is native to Southern Europe and is cultivated worldwide. The essential oil of clary sage was analyzed as a potential antifungal agent. The main compounds in the oil were linalyl acetate (52.83%) and linalool (18.18%). Food poisoning agents, spoilage fungi, and plant and animal pathogens were among the tested fungal species. The microdilution method was used to establish minimal inhibitory concentrations (MIC) and minimal fungicidal concentrations (MFC). The commercial antimycotic bifonazole was used as a control. A concentration of 25 ?l/ml showed fungicidal activity against Aspergillus, Penicillium, and Fusarium species and Trichoderma viride. For the species Mucor mucedo and Aspergillus viride, the MFC was 15?l/ml; for Candida albicans, it was 10 ?l/ml, as in the case of bifonazole. Fungistatic and fungicidal activities of the oil against Cladosporium cladosporioides and Trichophyton menthagrophytes were recorded at concentrations of 2.5 ?l/ml and 5 ?l/ml. The most sensitive micromycetes were Cladosporium fulvum, Alternaria alternata, Phomopsis helianthi, and Phoma macdonaldii, where a concentration of 2.5 ?l/ml was lethal.

scholarly journals Chemical Composition and Antimicrobial Activity of Anthriscus nemorosa Root Essential Oil

2011 ◽  
Vol 6 (2) ◽  
pp. 1934578X1100600
Author(s):  
Milica Pavlović ◽  
Silvana Petrović ◽  
Marina Milenković ◽  
Maria Couladis ◽  
Olga Tzakou ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Gram Positive Bacteria ◽  
Microdilution Method ◽  
Broth Microdilution Method ◽  
Bacillus Subtilis Atcc ◽  
Germacrene D ◽  
Main Components ◽  
Total Oil ◽  
Root Essential Oil

The essential oil obtained by hydrodistillation from the roots of Anthriscus nemorosa (Bieb.) Sprengel (Umbelliferae) was analyzed by GC and GC-MS. Among sixty-two compounds identified (representing 89.0% of the total oil), the main components were: n-nonane (12.1%), n-hexadecanol (6.9%), δ-cadinene (6.4%), β-pinene (6.0%) and germacrene D (5.4%). Furthermore, the antimicrobial activity of the oil was evaluated against the Gram-positive bacteria Staphylococcus epidermidis (ATCC 12228) and Bacillus subtilis (ATCC 6633), the Gramnegative bacterium Escherichia coli (ATCC 25922), and a yeast Candida albicans (ATCC 10259 and ATCC 24433) using the broth microdilution method.

scholarly journals Composition and Antimicrobial Activity ofEuphrasia rostkovianaHayne Essential Oil

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Pavel Novy ◽  
Hana Davidova ◽  
Cecilia Suqued Serrano-Rojero ◽  
Johana Rondevaldova ◽  
Josef Pulkrabek ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Bacterial Infections ◽  
Antimicrobial Effect ◽  
Main Constituent ◽  
Hexadecanoic Acid ◽  
Eye Infections ◽  
Gram Positive Bacteria ◽  
Health Disorders

Eyebright,Euphrasia rostkovianaHayne (Scrophulariaceae), is a medicinal plant traditionally used in Europe for the treatment of various health disorders, especially as eyewash to treat eye ailments such as conjunctivitis and blepharitis that can be associated with bacterial infections. SomeEuphrasiaspecies have been previously reported to contain essential oil. However, the composition and bioactivity ofE. rostkovianaoil are unknown. Therefore, in this study, we investigated the chemical composition and antimicrobial activity of the eyebright essential oil against some organisms associated with eye infections:Enterococcus faecalis,Escherichia coli,Klebsiella pneumoniae,Staphylococcus aureus,S. epidermidis,Pseudomonas aeruginosa, andCandida albicans. GC-MS analysis revealed more than 70 constituents, with n-hexadecanoic acid (18.47%) as the main constituent followed by thymol (7.97%), myristic acid (4.71%), linalool (4.65%), and anethole (4.09%). The essential oil showed antimicrobial effect against all organisms tested with the exception ofP. aeruginosa. The best activity was observed against all Gram-positive bacteria tested with the minimum inhibitory concentrations of 512 µg/mL. This is the first report on the chemical composition ofE. rostkovianaessential oil and its antimicrobial activity.

Antimicrobial Activity and Composition of the Essential Oil of Gontscharovia popovii from Iran

2006 ◽  
Vol 61 (9-10) ◽  
pp. 681-684 ◽  
Author(s):  
Ali Sonboli ◽  
Fatemeh Sefidkon ◽  
Morteza Yousefzadi
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Essential Oil ◽  
Staphylococcus Epidermidis ◽  
Gram Negative Bacteria ◽  
Capillary Gc ◽  
Aerial Parts ◽  
Full Flowering ◽  
Strong Antimicrobial Activity

AbstractThe aerial parts of Gontscharovia popovii (B. Fedtsch. and Gontsch.) Boriss. were collected at full flowering stage. The essential oil was isolated by hydrodistillation and analyzed by a combination of capillary GC and GC-MS. Thirty-one components were identified with the main constituent being carvacrol (71.9%), followed by linalool (5.5%), p-cymene (4.5%) and γ-terpinene (4.4%). The in vitro antimicrobial activity of the essential oil of G. popovii was studied against seven Gram-positive and Gram-negative bacteria (Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae) and three fungi (Candida albicans, Saccharomyces cerevisiae and Aspergillus niger). The results of the bioassays showed that the oil exhibited strong antimicrobial activity against all the tested fungi and bacteria except for the resistant bacterium Pseudomonas aeruginosa.

scholarly journals Monitoring of Antimicrobial Activity of Essential Oils Using Molecular Markers

2009 ◽  
Vol 3 (1) ◽  
pp. 103-107 ◽  
Author(s):  
Hend A. Hamedo
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Antimicrobial Agents ◽  
Random Amplified Polymorphic Dna ◽  
Antimicrobial Effect ◽  
E Coli ◽  
Specific Effects ◽  
And Staphylococcus Aureus ◽  
Rosemary Essential Oil

Technological application of essential oils, as natural antimicrobial agents, to reduce the effect of pathogenic microorganisms, requires new methods of detection. The present work evaluated the parameters of antimicrobial activity of the essential oils of rosemary (Rosmarinus officinalis) on two pathogenic strains Escherichia coli and Staphylococcus aureus. The MBC and MIC values were of 2.5, 25 μl ml-1, and values of 1.25 and 5 μl ml-1 for the two strains respectively. In this study, an attempt has been made to evaluate randomly amplified polymorphic DNA (RAPD) analysis for its potential to establish antimicrobial effect of rosemary essential oil. For the preliminary assessment, this study compared the effects occurring at molecular levels in E. coli and Staph. aureus exposed to rosemary essential oil at the MIC concentrations for the two organisms. The qualitative modifications arising in random amplified polymorphic DNA (RAPD) profiles as a measure of DNA effects were compared with control which showed many differences. In conclusion, the measurement of parameters at molecular levels is valuable for investigating the specific effects of agents interacting with DNA.

Chemical Composition and Antimicrobial Activity of Teucrium Capitatum L. Subsp. Lusitanicum (Schreb.) T. Navarro & Rosua Essential Oil

2020 ◽  
Author(s):  
Haïfa Debbabi ◽  
Ridha El Mokni ◽  
Ibrahim Jlassi ◽  
Rajesh K Joshi ◽  
Saoussen Hammami
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Antimicrobial Properties ◽  
Significant Activity ◽  
Bacterial Strains ◽  
Gram Positive Bacteria ◽  
Sesquiterpene Hydrocarbons ◽  
Germacrene D

Abstract Chemical composition and antimicrobial activity of Teucrium capitatum L. subsp. lusitanicum essential oil was investigated for the first time in the present study. Qualitative and quantitative analyses of the chemical composition by gas chromatography and mass spectrometry (GC–FID and GC–MS) revealed the presence of 60 compounds representing 97.6% of the whole constituents. The main compounds were germacrene D (47.1%), spathulenol (5.8%), α-selinene (5.3%), germacrene A (2.9%), δ-cadinene (2.8%) and cubenol (2.7%). In vitro, the antimicrobial activity was investigated against five bacterial strains along with the yeast Candida albicans using broth microdilution assay. T. capitatum subsp. lusitanicum essential oil showed significant activity against the gram-positive bacteria Staphylococcus aureus (MIC = MBC = 78 μg mL−1), Bacillus subtilis (MIC = MBC = 156 μg mL−1) and the yeast C. albicans (MIC = MFC = 156 μg mL−1). The great potential of antimicrobial effects is most likely due to the very high percentage of sesquiterpene hydrocarbons particularly to germacrene D, for which the antimicrobial properties have been previously reported.

scholarly journals Biological Activity and Composition of the Essential Oil of Tetrataenium Nephrophyllum (Apiaceae) from Iran

2007 ◽  
Vol 2 (12) ◽  
pp. 1934578X0700201 ◽  
Author(s):  
Ali Sonboli ◽  
Mohammad Reza Kanani ◽  
Morteza Yousefzadi ◽  
Mehran Mojarrad
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Gram Negative Bacteria ◽  
Flowering Stage ◽  
High Antimicrobial Activity ◽  
Aerial Parts ◽  
Sesquiterpene Hydrocarbons ◽  
Germacrene D ◽  
Aliphatic Esters ◽  
Total Oil

The aerial parts of Tetrataenium nephrophyllum were collected at the flowering stage, hydrodistilled, and the essential oil was analyzed by GC and GC-MS. Forty components accounting for 97.9% of the total oil were identified. Germacrene D (38.5%), 2-ethylhexyl acetate (11.2%), n-octyl 2-methylbutanoate (9.2%) and geranyl isovalerate (8.3%) were the major constituents. Sesquiterpene hydrocarbons (51.3%) and aliphatic esters (40.4%) were found to be the main group of compounds. The antimicrobial activity of the essential oil of T. nephrophyllum was determined against seven Gram-positive and Gram-negative bacteria (Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus, S. epidermidis, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae), as well as three fungi (Candida albicans, Saccharomyces cerevisiae and Aspergillus niger). The bioassay showed that the oil exhibited moderate to high antimicrobial activity.

scholarly journals Evaluation of Clausena anisata Essential Oil from Cameroon for Controlling Food Spoilage Fungi and its Potential Use as an Antiradical Agent

2011 ◽  
Vol 6 (9) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Aoudou Yaouba ◽  
Léopold Ngoune Tatsadjieu ◽  
Pierre Michel Jazet Dongmo ◽  
François Xavier Etoa ◽  
Carl Moses Fontum Mbofung ◽  
...  
Keyword(s):  
Essential Oil ◽  
Antiradical Activity ◽  
Fungal Growth ◽  
Diffusion Method ◽  
Germacrene D ◽  
Food Spoilage Fungi ◽  
Spoilage Fungi ◽  
Main Components ◽  
Fungal Growth Inhibition ◽  
Potential Use

Investigations were conducted to determine the chemical composition, antifungal and antiradical activities of the essential oil extracted from the fresh leaves of Clausena anisata (Willd.) Hook. F. ex Benth (from Cameroon) against Aspergillus flavus, A. niger, A. parasiticus and Fusarium moniliforme. The essential oil obtained by hydrodistillation was analysed by GC and GC/MS. The disc diffusion method was used to evaluate the fungal growth inhibition at various concentrations of the oil while the antiradical activity of the essential oil was studied by the DPPH (diphenyl picryl hydrazyl) method. The main components obtained were E-ocimenone (15.1%), Z-ocimenone (11.5%), γ-terpinene (11.4%) and germacrene D (10.9%). After 10 days of incubation on essential oil supplemented medium, the growth of A. flavus, A. niger, A. parasiticus and F. moniliforme were totally inhibited by 4, 5, 5 and 5 mg/mL of C. anisata essential oil, respectively. The antiradical activity of C. anisata essential oil (SC50 = 5.1 g/L) was less than that of butylated hydroxyl toluene (BHT), which was used as the reference compound (SC50 = 0.007 g/L). Results obtained in the present study indicate the possibility of exploiting C. anisata essential oil to fight strains of A. flavus, A. niger, A. parasiticus and F. moniliforme responsible for biodeterioration of stored food products.

scholarly journals Essential Oil from the Leaves of Annona vepretorum: Chemical Composition and Bioactivity

2012 ◽  
Vol 7 (2) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Emmanoel Vilaça Costa ◽  
Lívia Macedo Dutra ◽  
Paulo Cesar de Lima Nogueira ◽  
Valéria Regina de Souza Moraes ◽  
Marcos José Salvador ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Trypanosoma Cruzi ◽  
Candida Tropicalis ◽  
Trypanocidal Activity ◽  
Germacrene D ◽  
Weak Activity ◽  
Mic Value ◽  
Type Apparatus

The essential oil from the leaves of Annona vepretorun was obtained by hydrodistillation using a Clevenger-type apparatus and analyzed by GC-MS and GC-FID. Eighteen compounds representing 98.1% of the crude essential oil were identified. The major compounds identified were bicyclogermacrene (43.7%), spathulenol (11.4%), α-felandrene (10.0%), α-pinene (7.1%), (E)-β-ocimene (6.8%), germacrene D (5.8%), and p-cymene (4.2%). The trypanocidal activity against Trypanosoma cruzi epimastigote forms, as well as, the antimicrobial and antioxidant proprieties was investigated. The essential oil showed a potent trypanocidal activity with IC50 value of 31.9±1.3μg.mL-1. For antimicrobial activity, the best result was observed against Candida tropicalis with a MIC value of 100μg.mL-1. For antioxidant capacity the essential oil showed weak activity.

scholarly journals Phytochemical constituents and antimicrobial activity of Salvia

2018 ◽  
Vol 47 (4) ◽  
pp. 847-854
Author(s):  
Seyed Mahdi Tabei ◽  
Ardalan Alizadeh
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Staphylococcus Epidermidis ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Methanolic Extracts ◽  
Germacrene D ◽  
Phytochemical Constituents ◽  
Free Radical Scavenging Assay

The essential oil of Salvia nemorosa L. was analyzed by GC-MS. The major constituents of the oil were E-caryophyllene (26.37%), phytol (16.92%), germacrene-D (15.34%), sabinene (12.86%), caryophyllene oxide (6.33%), bicyclogermacrene (4.71%) and n-tetradecane (2.45%). The total phenolic content and the antioxidant activity of methanolic extracts were determined with the Folin-Ciocalteau reagent and by the 2,2- diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay, respectively. The antimicrobial activity of the essential oil was individually evaluated against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli and Candida albicans using disc diffusion and serial dilution methods. The oil showed good antimicrobial activity against four medically important pathogens.

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