Evaluation of Clausena anisata Essential Oil from Cameroon for Controlling Food Spoilage Fungi and its Potential Use as an Antiradical Agent

Investigations were conducted to determine the chemical composition, antifungal and antiradical activities of the essential oil extracted from the fresh leaves of Clausena anisata (Willd.) Hook. F. ex Benth (from Cameroon) against Aspergillus flavus, A. niger, A. parasiticus and Fusarium moniliforme. The essential oil obtained by hydrodistillation was analysed by GC and GC/MS. The disc diffusion method was used to evaluate the fungal growth inhibition at various concentrations of the oil while the antiradical activity of the essential oil was studied by the DPPH (diphenyl picryl hydrazyl) method. The main components obtained were E-ocimenone (15.1%), Z-ocimenone (11.5%), γ-terpinene (11.4%) and germacrene D (10.9%). After 10 days of incubation on essential oil supplemented medium, the growth of A. flavus, A. niger, A. parasiticus and F. moniliforme were totally inhibited by 4, 5, 5 and 5 mg/mL of C. anisata essential oil, respectively. The antiradical activity of C. anisata essential oil (SC50 = 5.1 g/L) was less than that of butylated hydroxyl toluene (BHT), which was used as the reference compound (SC50 = 0.007 g/L). Results obtained in the present study indicate the possibility of exploiting C. anisata essential oil to fight strains of A. flavus, A. niger, A. parasiticus and F. moniliforme responsible for biodeterioration of stored food products.

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Identifikasi komponen dan Uji Potensi Anti Jamur Minyak Atsiri Serai Dapur (Cympobogon citratus) Terhadap Jamur Penghasil Aflatoksin

AGRITEKNO Jurnal Teknologi Pertanian ◽

10.30598/jagritekno.2020.9.2.72 ◽

2020 ◽

Vol 9 (2) ◽

pp. 72-80

Author(s):

Rini Yanti ◽

Hermina Nurdiawati ◽

Muhammad N Cahyanto ◽

Yudi Pranoto

Keyword(s):

Essential Oil ◽

Aspergillus Flavus ◽

Fungal Growth ◽

Food Spoilage ◽

Cymbopogon Citratus ◽

Lemongrass Oil ◽

Food Spoilage Fungi ◽

Spoilage Fungi ◽

Main Components ◽

Lemongrass Essential Oil

Aspergillus flavus and A. parasiticus are food spoilage fungi and produce aflatoxin that causes serious food safety problems. The antifungal agent is needed to control these fungi. This study aimed to determine the component of lemongrass essential oil and to test the oil’s ability to inhibit the growth of A. flavus and A. parasiticus. Five variations in the concentration of lemongrass oil were tested separately on A. flavus and A. parasiticus and, fungal growth was observed for 7 days. The results of the identification of the essential oil components using GC-MS showed that lemongrass essential oil has the main components of citral (53.77%) and z-citral (34.05%). Lemongrass essential oil with various concentrations (0.01%; 0.02%; 0.04% and 0.08%) showed the ability to inhibit the growth of A. flavus and A. parasiticus and the concentration of 0.08% indicated that lemongrass oil could inhibit the growth of both fungi for 100% during 7 days of observation. Keywords: antifungal, aflatoxin, citral, essential oil, lemongrass oil ABSTRAK Aspergillus flavus dan A. parasiticus merupakan jamur yang banyak ditemukan mengkontaminasi pangan. Kedua jamur tersebut menghasilkan aflatoksin, yang berbahaya bagi kesehatan manusia, sehingga diperlukan anti-jamur untuk mengendalikan pertumbuhannya. Penelitian ini bertujuan untuk mengetahui komponen minyak atsri serai (Cymbopogon citratus) dan menguji kemampuan minyak tersebut sebagai penghambat pertumbuhan A. flavus dan A. parasiticus. Lima variasi konsentrasi minyak serai diujikan secara terpisah pada A. flavus dan A. parasiticus dan dilakukan pengamatan pertumbuhan jamur selama 7 hari. Hasil identifikasi komponen minyak atsiri menggunakan GC-MS menunjukkan bahwa minyak atsri serai memiliki komponen utama citral (53,77%) dan z-citral (34,05%). Minyak atsiri serai dengan berbagai konsentrasi (0,01%; 0,02%; 0,04% dan 0,08%) menunjukkan kemampuan menghambat pertumbuhan A. flavus dan A. parasiticus dan konsentrasi 0,08 % menunjukkan bahwa minyak serai mampu menghambat pertumbuhan kedua jamur sebesar 100 % selama 7 hari pengamatan Kata kunci: antijamur, aflatoksin, minyak atsiri, minyak serai, sitral

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Chemical composition and antimicrobial activity of the essential oil from twigs and leaves of Magnolia macclurei (Dandy) Figlar from Ha Giang Province, Vietnam

TAP CHI SINH HOC ◽

10.15625/0866-7160/v42n1.13839 ◽

2020 ◽

Vol 42 (1) ◽

Author(s):

Chu Thi Thu Ha ◽

Tran Huy Thai ◽

Le Ngoc Diep ◽

Dinh Thi Thu Thuy ◽

Nguyen Duc Ky ◽

...

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Diffusion Method ◽

Gram Negative Bacteria ◽

Minor Components ◽

Inhibitory Zone ◽

Disk Diffusion Method ◽

Gram Positive Bacteria ◽

Germacrene D ◽

Main Components

The chemical composition of the essential oil obtained by hydrodistillation of the twigs and leaves of Magnolia macclurei collected in Hoang Su Phi, Ha Giang Province, Vietnam was analyzed using GC/MS. A total of 38 compounds were detected in essential oil, accounting for 94.49% of the oil, in which 37 compounds were identified accounting for 93.33%. cis-β-Elemene (14,30%), bicyclogermacrene (18,57%), and 10-epi-ᵧ-eudesmol (16,86%) were the main components of essential oil. The minor components present with rather high amount were: Germacrene D (2,12%), guaiol (8,52%), hinesol (2,34%), valerianol (6,76%), and bulnesol (2,93%). Antibiotic activity of the essential oil sample was tested against a Gram positive bacteria Staphylococcus aureus, a Gram negative bacteria Escherichia coli, and a yeast Candida albicans using an agar disk diffusion method. All three microorganisms tested are resistant to the oil from twigs and leaves of M. macclurei. That is exhibited through the inhibitory zone diameters ranging from 4 to 12 mm.

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ANTIRADICAL ACTIVITY OF ESSENTIAL OILS OF FILIPENDULA ULMARIA (L.) MAXIM, HYPERICUM PERFORA-TUM L. AND PULMONARIA MOLLIS WULFEN EX HORNEM OF THE KRASNOYRSK TERRITORI FLORA

chemistry of plant raw material ◽

10.14258/jcprm.2021039195 ◽

2021 ◽

pp. 211-217

Author(s):

Irina Dement'yevna Zykova ◽

Aleksandr Alekseyevich Efremov

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Antiradical Activity ◽

Component Composition ◽

Salicylic Aldehyde ◽

Filipendula Ulmaria ◽

Antiradical Properties ◽

Hypericum Perforatum L ◽

Butyl Phthalate ◽

Main Components

The antiradical properties of essential oils from the inflorescences of Filipendula ulmaria (L). Maxim, herbages Hypericum perforatum L. and Pulmonaria mollis Wulfen ex HORNEM., growing on the territory of the Krasnoyarsk territory were studied. For this purpose, the reaction of essential oil components with a stable free 2,2-diphenyl-1-picrylhydrazyl radical was used. Essential oil of the plants under study received comprehensive hydroponically. The component composition of the oils was determined by chromatography-mass spectrometry. The main components of essential oil of F. ulmaria inflorescences are methyl salicylate (28.2%), salicylic aldehyde (2.8 %) and linalool (4.9%), essential oil of H. perforatum – γ - amorphene (30.7%), δ-cadinen (7.1%), (E, E)-β-farnesene (5.5%), caryophyllene (5.0%), ledol (5.0%), essential oil of P. mollis – di-n-butyl phthalate (18.7%), docosan (13.4%), tetracosan (11.6 %). The results of the DPPH test showed that the essential oils of the inflorescences of F. ulmaria and the aboveground part of H. perforatum and P. mollis exhibit antiradical activity (ARA). According to the size of the ARA of essential oils, the studied plants can be arranged in the following row: P. mollis > F. ulmaria > H. perforatum.

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Chemical Composition and Biological Activity of the Essential Oil from Jamaican Cleome serrata

Natural Product Communications ◽

10.1177/1934578x1200700934 ◽

2012 ◽

Vol 7 (9) ◽

pp. 1934578X1200700 ◽

Cited By ~ 1

Author(s):

Megil J. McNeil ◽

Roy B. R. Porter ◽

Lawrence A. D. Williams

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Antimicrobial Activities ◽

Diffusion Method ◽

Oil Composition ◽

Filter Paper Disc ◽

Aerial Parts ◽

Main Components ◽

Total Oil

The chemical composition of the essential oil obtained from the aerial parts of Cleome serrata by hydrodistillation was analyzed by employing GC-FID, GC-MS and RI. Fourteen compounds comprising 90.4% of the total oil composition were characterized. The main components identified were ( Z)-phytol (53.0%) and di(2-ethylhexyl)-phthalate (DEHP) (14.7%). The oil was evaluated for its in vitro antimicrobial activities against nine pathogenic microorganisms using the filter paper disc diffusion method. Moderate antimicrobial activity was observed against five of the pathogens assayed. In addition, the essential oil was tested against the sweet potato weevil, Cylas formicarius elegantulus. Strong knockdown insecticidal activity was observed.

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Chemical Composition and Antimicrobial Activity of Anthriscus nemorosa Root Essential Oil

Natural Product Communications ◽

10.1177/1934578x1100600229 ◽

2011 ◽

Vol 6 (2) ◽

pp. 1934578X1100600

Author(s):

Milica Pavlović ◽

Silvana Petrović ◽

Marina Milenković ◽

Maria Couladis ◽

Olga Tzakou ◽

...

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Gram Positive Bacteria ◽

Microdilution Method ◽

Broth Microdilution Method ◽

Bacillus Subtilis Atcc ◽

Germacrene D ◽

Main Components ◽

Total Oil ◽

Root Essential Oil

The essential oil obtained by hydrodistillation from the roots of Anthriscus nemorosa (Bieb.) Sprengel (Umbelliferae) was analyzed by GC and GC-MS. Among sixty-two compounds identified (representing 89.0% of the total oil), the main components were: n-nonane (12.1%), n-hexadecanol (6.9%), δ-cadinene (6.4%), β-pinene (6.0%) and germacrene D (5.4%). Furthermore, the antimicrobial activity of the oil was evaluated against the Gram-positive bacteria Staphylococcus epidermidis (ATCC 12228) and Bacillus subtilis (ATCC 6633), the Gramnegative bacterium Escherichia coli (ATCC 25922), and a yeast Candida albicans (ATCC 10259 and ATCC 24433) using the broth microdilution method.

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Essential Oil from Leaves of Lantana canescens and L. lopez-palacii Grown in Colombia

Natural Product Communications ◽

10.1177/1934578x1100600223 ◽

2011 ◽

Vol 6 (2) ◽

pp. 1934578X1100600

Author(s):

Andrés F. Peralta-Bohórquez ◽

Clara Quijano-Célis ◽

Mauricio Gaviria ◽

Consuelo Vanegas-López ◽

Jorge A. Pino

Keyword(s):

Essential Oil ◽

Volatile Compounds ◽

Diffusion Method ◽

Disc Diffusion ◽

Enterobacter Sakazakii ◽

Bacterial Strains ◽

Disc Diffusion Method ◽

Germacrene D ◽

In Vitro Antibacterial Activity

The chemical composition of the volatile compounds from the leaves of Lantana canescens Kunth (Verbenaceae) and L. lopez-palacii Moldenke grown in Colombia were analyzed by GC and GC/MS. One hundred and thirty-nine volatile compounds were identified in L. canescens, of which the major ones were β-caryophyllene (13.5%), germacrene D (10.3%) and 1-octen-3-ol (8.4%). In the oil obtained from L. lopez-palacii, eighty-three compounds were identified, of which the most prominent were 1-octen-3-ol (24.4%) and β-caryophyllene (15.2%). The in vitro antibacterial activity of the L. lopez-palacii essential oil was studied against three bacterial strains using the disc diffusion method. No antimicrobial activity was found against Escherichia coli, Enterobacter sakazakii and Listeria monocytogenes.

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Chemical Composition of the Essential Oil from Chaerophyllum Temulum (Apiaceae)

Natural Product Communications ◽

10.1177/1934578x1501000832 ◽

2015 ◽

Vol 10 (8) ◽

pp. 1934578X1501000 ◽

Cited By ~ 1

Author(s):

Jelena G. Stamenković ◽

Gordana S. Stojanović ◽

Ivana R. Radojković ◽

Goran M. Petrović ◽

Bojan K Zlatković

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Quantitative Composition ◽

Qualitative Composition ◽

Flowering Stage ◽

Main Compound ◽

Fruiting Stage ◽

Sesquiterpene Hydrocarbons ◽

Germacrene D ◽

Main Components

The present study reports the chemical composition on the essential oil obtained from fresh roots, stems, inflorescences and fruits of Chaerophyllum temulum. In all samples, except the roots, the most dominant components were sesquiterpene hydrocarbons. ( Z)-Falcarinol was the principal constituent of the root essential oils (61.7% at the flowering stage and 62.3% at the fruiting stage). The blossom oil was dominated by ( Z,E)-α-farnesene (23.4%), ( E)-β-farnesene (9.0%) and germacrene D-4-ol (9%), whereas the oil from the fruit had germacrene D-4-ol (27.6%) as its main compound, accompanied by ( Z,E)-α-farnesene (13.4%). Germacrene D was the most abundant component of the stem essential oil (38.4% at the flowering stage and 32.5% at the fruiting stage). The obtained results show that the qualitative composition of the oil depends on the part of the plant which is analyzed, while the quantitative composition of the main components depends on the growing stage of the plant.

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Antifungal Activity of Essential Oil ofEucalyptus camaldulensisDehnh. against SelectedFusariumspp.

International Journal of Microbiology ◽

10.1155/2017/8761610 ◽

2017 ◽

Vol 2017 ◽

pp. 1-7 ◽

Cited By ~ 22

Author(s):

Martin Muthee Gakuubi ◽

Angeline W. Maina ◽

John M. Wagacha

Keyword(s):

Minimum Inhibitory Concentration ◽

Antifungal Activity ◽

Essential Oil ◽

Mycelial Growth ◽

Inhibitory Concentration ◽

Eucalyptus Camaldulensis ◽

Gas Chromatography Mass Spectrometry ◽

Diffusion Method ◽

Minimum Fungicidal Concentration ◽

Potential Use

The objective of this study was to evaluate the antifungal activity of essential oil (EO) ofEucalyptus camaldulensisDehnh. against fiveFusariumspp. commonly associated with maize.The essential oil had been extracted by steam distillation in a modified Clevenger-type apparatus from leaves ofE. camaldulensisand their chemical composition characterized by gas chromatography mass spectrometry. Poisoned food technique was used to determine the percentage inhibition of mycelial growth, minimum inhibitory concentration, and minimum fungicidal concentration of the EO on the test pathogens. Antifungal activity of different concentrations of the EO was evaluated using disc diffusion method. The most abundant compounds identified in the EO were 1,8-cineole (16.2%),α-pinene (15.6%),α-phellandrene (10.0%), and p-cymene (8.1%). The EO produced complete mycelial growth inhibition in all the test pathogens at a concentration of 7-8 μL/mL after five days of incubation. The minimum inhibitory concentration and minimum fungicidal concentration of the EO on the test fungi were in the range of 7-8 μL/mL and 8–10 μL/mL, respectively. These findings confirm the fungicidal properties ofE. camaldulensisessential oils and their potential use in the management of economically importantFusariumspp. and as possible alternatives to synthetic fungicides.

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Productivity of some varieties of Origanum vulgare L. in the Foothill zone of the Crimea

PROCEEDINGS OF V INTERNATIONAL SCIENTIFIC CONFERENCE “CURRENT STATE, PROBLEMS AND PROSPECTS OF THE DEVELOPMENT OF AGRARIAN SCIENCE” ◽

10.33952/2542-0720-2020-5-9-10-70 ◽

2020 ◽

Author(s):

E.F. Myagkikh ◽

◽

I.N. Korotkikh ◽

Keyword(s):

Essential Oil ◽

Dry Weight ◽

Origanum Vulgare ◽

Medicinal And Aromatic Plants ◽

Russian Scientific ◽

Russian Scientific Research Institute ◽

Germacrene D ◽

The Crimea ◽

Economically Valuable Traits ◽

Main Components

The purpose of the research is a comparative study of the Origanum vulgare L. varieties ‘Raduga’, ‘Zima’, ‘Slavnitsa’ bred in the FSBSI All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (VILAR) according to morphological and biological characteristics and economically valuable traits under conditions of the Foothill zone of the Crimea. The productivity of the aforementioned varieties amounted to 1.05±0.2–1.90±0.3 kg/m2; mass fraction of essential oil – 0.04±0.06–0.06±0.24 % of fresh weight and 0.10 ±0.55–0.17±0.61% of absolutely dry weight; essential oil collection – 0.4–0.9 g/m2. Germacrene D (18.4–19.5%) and β-caryophyllene (18.8–27.0%) are the main components of the essential oil collected from some varieties of Origanum vulgare L.

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Chemical composition and antibacterial activity of essential oils of Thymelaea hirsuta from Algeria

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d201012 ◽

2019 ◽

Vol 20 (10) ◽

Author(s):

Souheila Bounab ◽

Takia Lograda ◽

Messaoud Ramdani ◽

Pierre Chalard ◽

Gilles Figueredo

Keyword(s):

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Diffusion Method ◽

Nonanoic Acid ◽

Upgma Cluster Analysis ◽

Oil Concentration ◽

Thymelaea Hirsuta ◽

Main Components

Abstract. Souhila B, Takia L, Messaoud R, Pierre C, Gilles F. 2019. Chemical composition and antibacterial activity of essential oils of Thymelaea hirsuta from Algeria. Biodiversitas 20: 2868-2876. The objectives of this study were to determine the chemical composition and to evaluate the antibacterial activity of Thymelaea hirsuta (L.) Endl., essential oils from seven sampling locations in M'sila region (Algeria). Extraction of essential oils was carried out by the hydro-distillation; the analysis of chemical composition of essential oil was carried out by GC-MS. Antimicrobial activity was performed by disc diffusion method at the essential oil concentration of non-diluted and diluted (1:2, 1:4 and 1:8 v:v of DMSO) against eight species of bacteria. The results showed that the average yields of essential oils were 0.3 ± 0.12%. A total of 45 components were identified, averaging 98.2 ± 1.85% of the total oils. The main components were nonanal-n (10.39 ± 3.21%), hexadecanoic acid (9.77 ± 2.81%), nonanoic acid (9.13 ± 6.49%), triacontane (7.2 ± 3.34%), isopropyl tetradecanoate (6.16 ± 1.99%) and tridecane (4.87 ± 3.1%). Based on the UPGMA cluster analysis, there were two clades of T. hirsuta. T. hirusta has a chemical polymorphism with different chemotypes marked in nature. There were four chemotypes identified in the essential oil of T. hirsuta in the region of M'sila. The essential oil of T. hirsuta has antibacterial activity against eight tested bacteria on the concentration-dependentt manner.

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