Chemical Composition, Antifungal, and Cytotoxicity Activities of Inga laurina (Sw.) Willd Leaves

The species Inga laurina is native to the Brazilian Cerrado. There are no studies about the chemical composition and biological activities of extracts of this endangered species. The ethanolic extract and its successive fractions are rich in phenolic compounds and presented good antifungal activities. HPLC/MS-MS/MS and H1/C13 analysis led to the identification of seventeen compounds, most of which are gallic acid derivatives, myricetin and quercetin glycosides. The ethyl acetate fraction (EAF) contained high levels of total phenolics, expressed in milligrams of gallic acid equivalents per gram of extract (475.3 ± 1.9 mg GAE gextract-1) and flavonoids expressed in milligrams of quercetin equivalents per gram of extract (359.3 ± 10.6 mg QE gextract-1). This fraction was active against fungi of the Candida genus. The EAF showed MIC value 11.7 μg mL−1 against C. glabrata and a selectivity index of 1.6 against Vero cells. The flavonol glycoside myricetin-3-O-rhamnoside was isolated for the first time from the Inga laurina. These results make I. laurina a promising plant as a source of pharmaceutical and biological active antifungal compounds.

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Flavonoid Constituents of Carduncellus Mareoticus (Del.) Hanelt and their Biological Activities

Natural Product Communications ◽

10.1177/1934578x0800300519 ◽

2008 ◽

Vol 3 (5) ◽

pp. 1934578X0800300

Author(s):

Marwan M. Shabana ◽

Moshera M. El-Sherei ◽

Mohamed Y. Moussa ◽

Amany A. Sleem ◽

Hosam M. Abdallah

Keyword(s):

Breast Cancer ◽

Cell Line ◽

Cancer Cell ◽

Breast Cancer Cell Line ◽

Biological Activities ◽

Cancer Cell Line ◽

Ethanolic Extract ◽

Ethyl Acetate Fraction ◽

Moderate Activity ◽

First Time

The total ethanolic extract (TEE) of Carduncellus mareoticus (Del.) Hanelt showed antioxidant and antihyperlipidemic activities, which were attributed to the ethyl acetate fraction (EAF). Also TEE showed potent cytotoxic activity against a cervix cancer cell line (IC50 = 5 μg/mL) and moderate activity against a breast cancer cell line. Chemical investigation of EAF led to the isolation of a new flavonoid, 8-hydroxy-5-methoxyluteolin 7- O-β-D-glucopyranoside (11), along with ten known metabolites (1–10). Three of these compounds, isorhamnetin 3- O-β-D-glucopyranoside (7), luteolin 3′- O-β-D-glucopyranoside (8), and eriodictyol 7- O-β-D-glucopyranoside (9) are reported for the first time from the genus Carduncellus, and two compounds, chrysoeriol 7- O-β-D-glucopyranoside (6), and luteolin 7- O-β-D-glucopyranoside (10), for the first time from C. mareoticus. The rest of the isolated compounds, chrysoeriol (1), luteolin (2), quercetin (3), kaempferol 3- O-α-L-arabinopyranoside (4), and kaempferol 3- O-β-D-glucopyranoside (5) have been previously reported from the genus.

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Pharmacological Potential and Chemical Composition of Crocus sativus Leaf Extracts

Molecules ◽

10.3390/molecules27010010 ◽

2021 ◽

Vol 27 (1) ◽

pp. 10

Author(s):

Olha Mykhailenko ◽

Vilma Petrikaite ◽

Michal Korinek ◽

Fang-Rong Chang ◽

Mohamed El-Shazly ◽

...

Keyword(s):

Chemical Composition ◽

Antioxidant Activities ◽

Biological Activities ◽

Ethanolic Extract ◽

Dry Weight ◽

Crocus Sativus ◽

Leaf Extracts ◽

Hplc Fingerprint ◽

Health Related ◽

Pharmacological Potential

Crocus sativus L. (saffron) has been traditionally used as a food coloring or flavoring agent, but recent research has shown its potent pharmacological activity to tackle several health-related conditions. Crocus sp. leaves, and petals are the by-products of saffron production and are not usually used in the medicine or food industries. The present study was designed to determine the chemical composition of the water and ethanolic extracts of C. sativus leaves and test their cytotoxic activity against melanoma (IGR39) and triple-negative breast cancer (MDA-MB-231) cell lines by MTT assay. We also determined their anti-allergic, anti-inflammatory, and anti-viral activities. HPLC fingerprint analysis showed the presence of 16 compounds, including hydroxycinnamic acids, xanthones, flavonoids, and isoflavonoids, which could contribute to the extracts’ biological activities. For the first time, compounds such as tectoridin, iristectorigenin B, nigricin, and irigenin were identified in Crocus leaf extracts. The results showed that mangiferin (up to 2 mg/g dry weight) and isoorientin (8.5 mg/g dry weight) were the major active ingredients in the leaf extracts. The ethanolic extract reduced the viability of IGR39 and MDA-MB-231 cancer cells with EC50 = 410 ± 100 and 330 ± 40 µg/mL, respectively. It was more active than the aqueous extract. Kaempferol and quercetin were identified as the most active compounds. Our results showed that Crocus leaves contain secondary metabolites with potent cytotoxic and antioxidant activities.

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A First Approach to the Chemical Composition and Antioxidant Potential of Guinea-Bissau Propolis

Natural Product Communications ◽

10.1177/1934578x19844138 ◽

2019 ◽

Vol 14 (5) ◽

pp. 1934578X1984413 ◽

Cited By ~ 1

Author(s):

Soraia I. Falcão ◽

Mélissa Lopes ◽

Miguel Vilas-Boas

Keyword(s):

Antioxidant Activity ◽

Chemical Composition ◽

Physicochemical Properties ◽

Natural Product ◽

Broad Spectrum ◽

Biological Activities ◽

Chemical Diversity ◽

Antioxidant Potential ◽

Phenolic Composition ◽

First Time

Propolis is a natural product derived from plant resins collected by honeybees and used in the beehive as a construction and defensive material. The broad spectrum of biological activities is dependent on the chemical diversity of its composition which is determined by the floral sources at the site of collection. For the first time, the chemical composition of Guinean propolis as well as its physicochemical properties, phenolic composition, and antioxidant activity was assessed. Eight compounds were identified through LC/DAD/ESI-MS n , mostly isoflavonoids, resembling Nigerian and Brazilian red propolis from the genus Dalbergia.

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Chemical Composition, Antioxidant and Cytotoxic Activities of Roots and Fruits of Berberis libanotica

Natural Product Communications ◽

10.1177/1934578x1601100523 ◽

2016 ◽

Vol 11 (5) ◽

pp. 1934578X1601100 ◽

Cited By ~ 2

Author(s):

Leina El Hosry ◽

Laurent Boyer ◽

Elnur E. Garayev ◽

Fathi Mabrouki ◽

Sok-Siya Bun ◽

...

Keyword(s):

Chemical Composition ◽

Chlorogenic Acid ◽

Gallic Acid ◽

Cell Line ◽

Antioxidant Activities ◽

Ht29 Cell ◽

Cytotoxic Activities ◽

First Time ◽

Ht29 Cell Line

Fourteen compounds belonging to different chemical classes were characterized in the roots and fruits extracts from Berberis libanotica, using the same HPLC-DAD-MS method. Thirteen were reported, for the first time, from the fruits whereas the roots contained mostly alkaloids of which 3 out of 5 are reported for the first time. Their structures were established on the basis of MS data as gallic acid (1), chlorogenic acid (2), delphinidin (3), oxyacanthine (4), rutin (5), hyperoside (6), berbamine (7), isoquercitrin (8), quercitrin (9), jatrorrhizine (10), palmatine (11), berberine (12), quercetin (13) and luteolin (14). Extracts containing compounds 4 and 7 showed significant cytotoxicity against the HT29 cell line with an IC50 of 12.2–26.1 μg/mL. Fruits extracts, due mostly to compounds 1 and 2, showed potent antioxidant activities with an EC50 of 0.0025-0.019 mg/mL.

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Investigation of Phenolic Constituents of Carduncellus eriocephalus Boiss. var. albiflora Gauba and their Biological Activities

Natural Product Communications ◽

10.1177/1934578x0700200807 ◽

2007 ◽

Vol 2 (8) ◽

pp. 1934578X0700200 ◽

Cited By ~ 1

Author(s):

Marwan M. Shabana ◽

Moshera M. El-Sherei ◽

Mohamed Y. Moussa ◽

Amani A. Sleem ◽

Hosam M. Abdallah

Keyword(s):

Biological Activities ◽

Ethanolic Extract ◽

Chemical Investigation ◽

Butanol Fraction ◽

Dicaffeoylquinic Acid ◽

Phenolic Constituents ◽

The Family ◽

First Time

The total ethanolic extract (TEE) of Carduncellus eriocephalus Boiss. var. albiflora Gauba showed antioxidant, antihyperglycaemic, and antihyperlipidaemic activities, which were attributed to the n-butanol fraction (BF). Chemical investigation of the BF led to the isolation of a new flavonoid, kaempferol-3- O-β-D-galactopyranoside-6″-sulfate (16), and a phenyl propenyl glycoside, 1-β-D-glucopyranoside-3-phenyl-2-propenol (rosin) (6), which is reported for the first time in the family Asteraceae. Chrysoeriol (1), 3′- O-methylorobol (2), luteolin (3), quercetin (4), myricetin (5), kaempferol 3- O-α-L-arabinopyranoside (7), kaempferol 3- O-β-D-glucopyranoside (8), quercetin 3- O-β-D-glucopyranoside (12), luteolin 7- O-β-D-rutinoside (13), quercetin 3- O-β-D-rutinoside (14), apigenin 7- O-β-D-rutinoside (15), and 3,5-dicaffeoylquinic acid (17) were isolated for the first time from the genus Carduncellus, in addition to apigenin 7- O-β-D-glucopyranoside (9), chryseriol 7- O-β-D-glucopyranoside (10) and luteolin 7- O-β-D-glucopyranoside (11), which have been previously reported for C. eriocephalus.

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Phytotoxicity of Schiekia timida Seed Extracts, a Mixture of Phenylphenalenones

Molecules ◽

10.3390/molecules26144197 ◽

2021 ◽

Vol 26 (14) ◽

pp. 4197

Author(s):

Fernanda Maria Marins Ocampos ◽

Ana Julia Borim de Souza ◽

Guilherme Medeiros Antar ◽

Felipe Christoff Wouters ◽

Luiz Alberto Colnago

Keyword(s):

Chemical Composition ◽

Methanolic Extract ◽

Biological Activities ◽

Germination Rate ◽

Seed Extract ◽

Hypocotyl Growth ◽

Specialized Metabolites ◽

Phytotoxic Effect ◽

First Time ◽

Seed Extracts

Phenylphenalenones, metabolites found in Schiekia timida (Haemodoraceae), are a class of specialized metabolites with many biological activities, being phytoalexins in banana plants. In the constant search to solve the problem of glyphosate and to avoid resistance to commercial herbicides, this work aimed to investigate the phytotoxic effect of the methanolic extract of S. timida seeds. The chemical composition of the seed extract was directly investigated by NMR and UPLC-QToF MS and the pre- and post-emergence phytotoxic effect on a eudicotyledonous model (Lactuca sativa) and a monocotyledonous model (Allium cepa) was evaluated through germination and seedling growth tests. Three concentrations of the extract (0.25, 0.50, and 1.00 mg/mL) were prepared, and four replicates for each of them were analyzed. Three major phenylphenalenones were identified by NMR spectroscopy: 4-hydroxy-anigorufone, methoxyanigorufone, and anigorufone, two of those reported for the first time in S. timida. The presence of seven other phenylphenalenones was suggested by the LC-MS analyses. The phenylphenalenone mixture did not affect the germination rate, but impaired radicle and hypocotyl growth on both models. The effect in the monocotyledonous model was statistically similar to glyphosate in the lowest concentration (0.25 mg/mL). Therefore, although more research on this topic is required to probe this first report, this investigation suggests for the first time that phenylphenalenone compounds may be post-emergence herbicides.

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Chemical Composition, Antioxidant, Antimicrobial and Antidiabetic Potential of Philodendron Bipinnatifidum Schott ex Endl

Journal of Food Engineering and Technology ◽

10.32732/jfet.2019.8.2.103 ◽

2019 ◽

Vol 8 (2) ◽

pp. 103-111

Author(s):

Jaqueline Scapinello ◽

Monica S. Z. Schindler ◽

Leila Zanatta ◽

Laura Cassol Mohr ◽

Ana Paula Capelezzo ◽

...

Keyword(s):

Chemical Composition ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Biological Activities ◽

Ethanolic Extract ◽

Antidiabetic Activity ◽

South American ◽

High Antimicrobial Activity ◽

Popular Medicine

Many of the species used in popular medicine do not have their biological activities already proven by scientific studies. Among these species, the endemic South American Philodendron bipinnatifidum Schott ex Endl deserves special attention since it is already in use in popular medicine for inflammation cases, such as erysipelas, orchitis and ulcers. This study evaluated the antioxidant, antimicrobial and antidiabetic activities of extracts of the hastes de P. bipinnatifidum. The ethanolic extract showed a significant antioxidant potential. The ethyl acetate extract resulted in high antimicrobial activity against Streptococcus pyogenes. The most significant biological activity of ethyl acetate extract relates to its chemical composition when compared with ethanolic extract, which showed the highest concentration of bioactive compounds. In vitro antidiabetic activity was only evaluated for ethyl acetate extract, resulting in inhibition of intestinal disaccharidases (maltase and sucrase) at concentration of 500 μg/mL.

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Extraction, Qualitative and Quantitative Determination of Secondary Metabolites of Rumex Nepalensis Roots

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v8i6-s.2092 ◽

2018 ◽

Vol 8 (6-s) ◽

pp. 97-100 ◽

Cited By ~ 1

Author(s):

Azher Nawaz Khan ◽

Irm Bhat

Keyword(s):

Quantitative Analysis ◽

Qualitative Analysis ◽

Total Phenolics ◽

Biological Activities ◽

Wide Spectrum ◽

Ethanolic Extract ◽

Food Supplement ◽

Aluminium Chloride ◽

Phytochemical Analysis ◽

Test Protocol

In the Indian ayurvedic system of medicine, Rumex nepalensis Spreng. (Polygonaceae) commonly known as Nepal Dock has wide-spectrum therapeutic potencies and is extensively used for centuries in traditional medicine systems. They act as a possible food supplement and are largely used in pharmaceutical industry. Extracts and metabolites from this plant exhibits pharmacological activities including anti-inflammatory, antioxidant, antibacterial, antifungal, antiviral, insecticidal, purgative, analesic, antipyretic, anti-algal, central nervous system depressant, genotoxic, wound healing and skeletal muscle relaxant activity. Due to its remarkable biological activities, it has the potential to act as a rich source of drug against life threatening diseases. The aim of the present study is to examine Rumex nepalensis roots for phytochemical profile. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenolics and flavonoids were determined by the well-known test protocol available in the literature. Quantitative analysis of phenolic and flavonoids was carried out by Folins Ciocalteau reagent method and aluminium chloride method respectively. Phytochemical analysis revealed the presence of phenols, flavonoids, tannins, saponins, alkaloids, fixed oil and fats. The total phenolics content of roots ethanolic extract was (1.658 mg/100mg), followed by flavonoids (1.048mg/100mg). The present study concluded that the crude extract of Rumex nepalensis is a potential source of various activates and this justifies its use in folkloric medicine. Keywords: Rumex nepalensis, Qualitative analysis, Quantitative analysis, TPC, TFC, Folins Ciocalteau

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Comparative Antioxidant Potential of Bark and Leaves of Terminalia arjuna (Roxb) Wight & Arn from Himachal Pradesh

International Journal of Pharmaceutical and Phytopharmacological Research ◽

10.24896/eijppr.2016615 ◽

2017 ◽

Vol 6 (1) ◽

pp. 27 ◽

Cited By ~ 4

Author(s):

Vikas Kumar ◽

Kamal Dev ◽

Anuradha Sourirajan ◽

Prem Kumar Khosla

Keyword(s):

Antioxidant Activity ◽

Gallic Acid ◽

Phenolic Content ◽

Total Phenolics ◽

Antioxidant Activities ◽

Ethanolic Extract ◽

Terminalia Arjuna ◽

Total Phenolic ◽

Flavonoid Content ◽

Phenolic And Flavonoid

The present study aimed to compare antioxidant activities, total phenolic and flavonoid content present in leaves and bark of Terminalia arjuna and check whether there is any correlation between phenolic content and flavonoid content with antioxidant activities or not. Phytochemical screening of ethanolic extract of leaves and bark revealed the presence of phenols, flavonoids, tannins, carbohydrates, glycosides, saponin, phytosterols and phytosteroids. Total phenolic content was found to be higher in bark (272.71±3.18 mg/g gallic acid equivalents) as compared to that of leaves (95±3.11 mg/g gallic acid equivalents). Similarly, flavonoid content of ethanolic extract of bark was found to be higher (203.95±5.13 mg/g rutin equivalents) than that of leaves (87.625±4.28 mg/g rutin equivalents). DPPH activity of ethanolic extract of bark (IC50-17.41 µg/ml) was more than that of leaves (IC50-20.22µg/ml). FRAP activity of bark (IC50- 4.781 µM Fe (II) equivalents) is more than that of leaves (IC50-7.572 µM Fe (II) equivalents). Nitric oxide (NO) scavenging activity of bark (IC50-12.87 µg/ml) was higher than that of leaves (IC50-13.91 µg/ml).The present study clearly showed that there is a correlation between total phenolics, flavonoid contents and antioxidant activity of leaves and bark of T. arjuna. The phenolic compounds and flavonoids could be major contributor to antioxidant activity of T. arjuna. Keywords: Terminalia arjuna, DPPH, FRAP, NO, IC50, Antioxidants

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Chemical Composition and Biological Activities from Croton delpyi Croton decalvatus and Croton caudatus

The Natural Products Journal ◽

10.2174/2210315510999200704143606 ◽

2020 ◽

Vol 10 ◽

Author(s):

Sakchai Chaibun ◽

Wilart Pompimon ◽

Chanika Tidchai ◽

Noraset Chalaemwongwan ◽

Jutarut Wongping ◽

...

Keyword(s):

Chemical Composition ◽

Biological Activities ◽

Chemical Constituents ◽

Antibacterial Activities ◽

Cytotoxic Activities ◽

Separation And Purification ◽

Pure Compounds ◽

Cytotoxicity Activities ◽

Anti Hiv ◽

Hiv 1

Background: C. delpyi, C. decalvatus and C. caudatus are in the Euphorbiaceae family. The aerial parts; twigs, leaves and barks of these plants were used as traditional medicine such as anti-inflammatory, cytotoxicity, and antifungal properties. Objectives: The aims of this work were 1) to study the chemical composition of C. delpyi, C. decalvatus, and C. caudatus 2) to test their antibacterial, anti-HIV-1 RT, and cytotoxicity activities of crude extracts and pure compounds from these plants. Methods: Extraction, separation and purification of three plants were performed under chromatographic method. The biological activities including antibacterial, anti-HIV-1 RT and cytotoxicity assay of three plants were evaluated by the standard methods. Results: Phytochemical investigation of C. delpyi was founded a new clerodanes diterpenoids; crotondelpyitin A (1). The five known compounds, such as acetyl aleuritolic acid (2), 5-hydroxy-7,4- dimethoxyflavone (3), and pilloin (4) were founded in C. decalvatus and 3α-benzoyloxy-D:A-friedo-oleanan-27,16αlactone (5), and bergenin (6) were founded in C. caudatus. The compound 3 show the most effective antibacterial activities with MIC in range <0.16 -1.25 mg/mL, and MBC in range 0.6 - >5.0 mg/mL. The six compounds were inactive with antiHIV-1 RT. In addition, compound 4 was active for cytotoxic activities on FaDu and KKU-M213 at <4 µg/mL. Conclusion: The present study reveals that the Croton species are sources of diterpenoid-type compounds and significant guide for further research of the chemical constituents from these plants as potential medicines.

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