Chemical Composition, Antioxidant, Antimicrobial and Insecticidal Activities of Essential Oil from a Moroccan Endemic Plant: Bubonium imbricatum

The chemical composition, antioxidant, antimicrobial and insecticidal properties of essential oil obtained by hydro-distillation from Bubonium imbricatum Cav. have been investigated. The essential oil of aerial parts was analyzed by GC-FID and GC-MS. A total of 63 compounds were identified representing more than 82% of the oil. The major components were cis-chrysanthenyl acetate (31.2%) and thymol isobutyrate (3.4%). The antioxidant activity was evaluated by the DPPH and reducing power test. The data showed moderate activity compared with the reference compounds. Our results showed strong activity of the investigated oil against all tested microorganisms. The highest antibacterial activity was observed against Bacillus subtilis (inhibition zone= 18 ± 0.21, MIC= 0.18 mg/mL). In general, Gram-positive bacteria were more susceptible than Gram-negative ones. Candida parapsilosis was the most sensitive among the Candida strains tested. LD50 and LD90 values were 33.1 and 53.1 ppm against 1-d-old larvae of Aedes aegypti L.

Download Full-text

Chemotypes and antibacterial activities of Inula viscosa essential oils from Algeria

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d210431 ◽

2020 ◽

Vol 21 (4) ◽

Author(s):

Abdelkader Ounoughi ◽

Messaoud Ramdani ◽

Takia Lograda ◽

Pierre Chalard ◽

Gilles Figueredo

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Bacterial Species ◽

Antibacterial Activities ◽

Diffusion Method ◽

Moderate Activity ◽

Phytochemical Study ◽

Aerial Parts ◽

Total Oil

Abstract. Ounoughi A, Ramdani M, Lograda T, Chalard P, Figueredo G. 2020. Chemotypes and antibacterial activities of Inula viscosa essential oils from Algeria. Biodiversitas 21: 1504-1517. The aim of this work is to investigate the chemical composition and the antibacterial activities of the essential oils from Inula viscosa (L.) Aiton. Samples were collected in the flowering stage, from different localities in Algeria. The aerial parts of I. viscosa were submitted to a hydro distillation. The chemical composition of the essential oil was analyzed by GC and GC/MS. The antibacterial activity of the essential oils was evaluated using the disc diffusion method against fifteen bacterial species. Fifty-eight compounds representing 98.93 ± 2.03% of the total oil was identified in I. viscosa. It was found that the chemical composition was dominated by the presence of the following major products: polygodial (19.8 ± 16.97%), phytol (12.3 ± 9.77%), fokienol (6.01 ± 3.43%), intermedeol neo (5.09 ± 2.38%), caryophyllene oxide (4.91 ± 3.03%), nerolidol-Z (4.46 ± 5.46%), nerolidol-E (4.24 ± 8.07%) and α-ionone iso methyl-E (3.72 ± 2.26%). The essential oil of I. viscosa has moderate activity against the bacteria tested. In contrast, the Escherichia coli ATCC 25922, Pseudomonon syringae ATCC 53543 and Enterococcus faecalis ATCC 49452 strains are resistant to I. viscosa essential oils. The phytochemical study of I. viscosa showed that it is rich in terpene compounds, with polygodial and phytol as major components. Three distinct chemotypes are highlighted. The (Polygodial-Intermedeol-neo-Phytol) chemotype of Salah Bey population and two chemotypes with Fokinol-polygodial and Fokinol-phytol. Moderate antibacterial activities of essential oils against the bacteria tested were found.

Download Full-text

Chemical composition and antimicrobial activity of essential oil from Pyrethrum pulchrum Ledeb.

Mongolian Journal of Chemistry ◽

10.5564/mjc.v19i45.1088 ◽

2018 ◽

Vol 19 (45) ◽

pp. 38-43 ◽

Cited By ~ 1

Author(s):

Uugangerel Erdenetsogt ◽

Choijamts Gotov ◽

Kerstin Voigt ◽

Stefan Bartram ◽

Wilhelm Boland ◽

...

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Diffusion Method ◽

Oil Composition ◽

Disk Diffusion Method ◽

Gram Positive Bacteria ◽

Aerial Parts ◽

Total Oil ◽

Sporidiobolus Salmonicolor

The chemical composition and antimicrobial activity of the essential oil from the aerial parts of Pyrethrum pulchrum Ledeb. were investigated. Dried plant material was hydro-distillated yielding 0.1% of essential oil. The oil was analyzed by GC-MS techniques. Fifty-five compounds were identified representing 99.7% of the total oil composition. Camphor was the predominant compound (33.9%) followed by linalool (21.1%) and α-pinene (9.0%). The antimicrobial activity of the oil was determined using the disk diffusion method against Gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus and Enterococcus faecalis), Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli), Mycobacterium vaccae and fungi (Candida albicans, Sporidiobolus salmonicolor and Penicillum notatum). The essential oil of P. pulchrum displays an intermediate activity against selected bacteria.

Download Full-text

Essential Oil Composition and Antimicrobial Activity of Ballota nigra L. ssp Foetida

Natural Product Communications ◽

10.1177/1934578x0900400429 ◽

2009 ◽

Vol 4 (4) ◽

pp. 1934578X0900400 ◽

Cited By ~ 1

Author(s):

Daniele Fraternale ◽

Anahi Bucchini ◽

Laura Giamperi ◽

Donata Ricci

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oil Composition ◽

Caryophyllene Oxide ◽

Oil Composition ◽

Gram Negative ◽

Gram Positive Bacteria ◽

Aerial Parts ◽

Germacrene D

The chemical composition of the essential oil of Ballota nigra L. ssp foetida obtained from the flowering aerial parts was analyzed by GC/MS. From the 37 identified constituents of the oil, β-caryophyllene (20.0%), germacrene D (18.0%) and caryophyllene oxide (15.0%) were the major components. The oil was active against both Gram-negative and Gram-positive bacteria as well as against three Candida species.

Download Full-text

Chemical Composition and Biological Activity of Pulicaria vulgaris Essential Oil from Iran

Natural Product Communications ◽

10.1177/1934578x1400901126 ◽

2014 ◽

Vol 9 (11) ◽

pp. 1934578X1400901 ◽

Cited By ~ 6

Author(s):

Javad Sharifi-Rad ◽

Abdolhossein Miri ◽

Seyedeh Mahsan Hoseini-Alfatemi ◽

Majid Sharifi-Rad ◽

William N. Setzer ◽

...

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Cell Lines ◽

Chemotherapeutic Agents ◽

Cytotoxic Activities ◽

Hep G2 ◽

E Coli ◽

Gram Positive Bacteria ◽

Aerial Parts ◽

Mcf 7

The present study investigated the chemical composition of the essential oil (EO) from aerial parts (flowering stage) of Pulicaria vulgaris Gaertn. by GC–MS. Also, the antimicrobial activity of the EO against Gram-positive bacteria ( Bacillus cereus and Staphylococcus aureus), Gram-negative bacteria ( Escherichia coli and Pseudomonas aeruginosa) and fungi ( Aspergillus niger and Candida albicans) was tested. In total, 23 compounds were recognized, accounting for 98.08 % of the EO. The main compounds in the EO were thymol (50.22%), p-menth-6-en-2-one (carvotanacetone, 20.2%), thymol isobutyrate (16.88%), menthan-2-one (4.31%), 1-methyl-1,2-propanedione (4.13%), 2,5-dimethoxy- p-cymene (4.01%), myrtenol (1.22%), linalool (1.1%), and β-myrcene (1.9%). Results of antibacterial test of P. vulgaris essential oil showed that all assayed concentrations significantly inhibited the growth of B. cereus, S. aureus, E. coli, and P. aeruginosa at P < 0.05. MIC for B. cereus, S. aureus, E. coli, P. aeruginosa was 17.5, 25.2, 19.4 and 33.2 μg/mL respectively; antifungal screening of the essential oil of P. vulgaris showed that the oil significantly inhibited the growth of A. niger and C. albicans (MIC = 15.5 and 9.9 μg/mL, respectively). Results of cytotoxicity assay showed that the essential oil exhibited a significant cytotoxic activity against both cell lines. In case of MCF-7 and Hep-G2 cell lines, IC50 of the essential oil were 5.36 and 7.16 μμg/ml, respectively. The potent antimicrobial and cytotoxic activities of the EO may be attributed to its high contents of thymol, carvotanacetone and thymol isobutyrate. Antimicrobial and antitumor chemotherapies are showing diminishing effectiveness because of emergence of drug-resistance. Hence, using efficient natural chemotherapeutic agents such as Pulicaria vulgaris essential oil with fewer side effects is an encouraging approach to fight cancer and infectious diseases in medicine, agriculture, food science and related fields.

Download Full-text

Chemical composition and antimicrobial activity of the essential oil from Satureja horvatii Silic (Lamiaceae)

Journal of the Serbian Chemical Society ◽

10.2298/jsc0807703l ◽

2008 ◽

Vol 73 (7) ◽

pp. 703-711 ◽

Cited By ~ 13

Author(s):

Branislava Lakusic ◽

Mihailo Ristic ◽

Violeta Slavkovska ◽

Jelena Antic-Stankovic ◽

Marina Milenkovic

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Micrococcus Luteus ◽

Antimicrobial Activities ◽

Maximum Activity ◽

Moderate Activity ◽

Aerial Parts ◽

Weak Activity

The present paper describes the chemical composition and antimicrobial activity of the essential oil of the endemic species Satureja horvatii Silic, collected in Montenegro. The essential oil was obtained from the aerial parts of the plant by hydrodistillation and analyzed by GC-MS. From the 34 compounds representing 100 % of the oil, the major compound was the phenolic monoterpene thymol (63.37 %). The oil contained smaller amounts of g-terpinene (7.49 %), carvacrol methyl ether (4.92 %), carvacrol (4.67 %), p-cymene (4.52%), a-terpinene (1.81 %), borneol (1.58 %), a-thujene (1.56 %), b-caryophyllene (1.55 %) and b-myrcene (1.44 %). The antimicrobial activity of the essential oil of S. horvatii was evaluated using the agar diffusion and broth microdilution methods. The essential oil exhibited antimicrobial activity to varying degrees against all the tested strains. The maximum activity of S. horvatii oil was observed against Gram-positive bacteria (Micrococcus luteus, Staphylococcus epidermidis, Staphylococcus aureus and Enterococcus faecalis) and against the yeast (Candida albicans). The oil exhibited moderate activity against the Gram-negative bacteria Escherichia coli and Klebsiella pneumoniae and weak activity against Pseudomonas aeruginosa. This study confirms that the essential oil of S. horvatii possesses antimicrobial activities in vitro against medically important pathogens.

Download Full-text

Chemical Composition, Antioxidant and Antibacterial Properties of Essential Oil from Ocotea Auriculiformis Kost. (Lauraceae) Leaves, Endemic of Madagascar

European Scientific Journal ESJ ◽

10.19044/esj.2017.v13n33p364 ◽

2017 ◽

Vol 13 (33) ◽

pp. 364 ◽

Cited By ~ 1

Author(s):

Andrianantenaina Rigobert ◽

Ralambondrahety Rahanira ◽

Andriambeloson Onja ◽

Rabehaja Delphin ◽

Ralamboranto Laurence ◽

...

Keyword(s):

Streptococcus Pneumoniae ◽

Chemical Composition ◽

Essential Oil ◽

Bacillus Cereus ◽

Antioxidant Properties ◽

Antibacterial Properties ◽

Quantitative Measure ◽

Inhibition Zone ◽

Endemic Plant

Plants constitute an important source of secondary metabolites in which essential oils are well-known for their use in various domains such as pharmacy, therapeutic, cosmetology and foods. In vitro antimicrobial and antioxidant properties of Ocotea auriculiformis Kost. (Lauraceae) leaves essential oil is demonstrated and its chemical composition is reported in the present study. The essential oil from Ocotea auriculiformis Kost. (Lauraceae) leaves, an endemic plant of Madagascar was extracted by hydrodistillation method. Chemical composition using GC, GC/ MS and NMR13C methods showed that the essential oil contained around 47 products in which 93.95% were identified. Known compounds are constituted by 74.7% of hydrocarbons and 19.25% of oxygenated products. The essential oil is rich in sesquiterpene and monoterpene. In vitro antibacterial capacity of the essential oil was assessed by disc method against human and food pathogens. Bacillus cereus and Streptococcus pneumoniae were very sensitive to the essential oil with 11 mm and 25 mm of inhibition zone respectively. The MIC of the essential oil was 1mg/mL for Bacillus cereus and 0.25 mg/mL for Streptococcus pneumoniae. MBC values were 2.5 mg/mL and 0.5 mg/mL, respectively. The ratio MBC/MIC for both strains was inferior to 4 concluding hence that the essential oil has bactericidal effect against the two sensitive strains. In vitro antioxidant capacity of the essential oil was performed according to qualitalive (TLC) and quantitative (measure of DPPH radical scavengening) methods. The essential oil showed antioxidant activity with IC50 value of 0.35 mg/mL.

Download Full-text

GC/MS Evaluation and In Vitro Antioxidant Activity of Essential Oil and Solvent Extracts of an Endemic Plant Used as Folk Remedy in Turkey:Phlomis bourgaeiBoiss.

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2013/293080 ◽

2013 ◽

Vol 2013 ◽

pp. 1-7 ◽

Cited By ~ 9

Author(s):

Cengiz Sarikurkcu ◽

M. Sabih Ozer ◽

Ahmet Cakir ◽

Mustafa Eskici ◽

Ebru Mete

Keyword(s):

Linoleic Acid ◽

Chemical Composition ◽

Essential Oil ◽

Water Extract ◽

Reducing Power ◽

Endemic Plant ◽

Folk Remedy ◽

Excellent Activity ◽

Chelating Effect

This study was outlined to examine the chemical composition of hydrodistilled essential oil and in vitro antioxidant potentials of the essential oil and different solvent extracts of endemicPhlomis bourgaeiBoiss. used as folk remedy in Turkey. The chemical composition of the oil was analyzed by GC and GC-MS, and the predominant components in the oil were found to beβ-caryophyllene (37.37%),(Z)-β-farnesene (15.88%), and germacrene D (10.97%). Antioxidant potentials of the solvent extracts and the oil were determined by four testing systems includingβ-carotene/linoleic acid, DPPH, reducing power, and chelating effect. Inβ-carotene/linoleic acid assay, all extracts showed the inhibition of more than 50% at all concentrations. In DPPH, chelating effect, and reducing power test systems, the water extract with 88.68%, 77.45%, and 1.857 (absorbance at 700 nm), respectively, exhibited more excellent activity potential than other extracts (hexane, ethyl acetate and methanol) and the essential oil at 1.0 mg/mL concentration. The amount of the total phenolics and flavonoids was the highest in this extract (139.50 ± 3.98 μg gallic acid equivalents (GAEs)/mg extract and 22.71 ± 0.05μg quercetin equivalents (QEs)/mg extract).

Download Full-text

Chemical composition and antibacterial effect of Smyrnium olusatrum L. Fruit Essential Oil

Mediterranean Journal of Chemistry ◽

10.13171/mjc10602006231292iz ◽

2020 ◽

Vol 10 (6) ◽

pp. 577

Author(s):

Saida Cherif Hamida ◽

Imane Zalegh ◽

Fairouz Saidi ◽

Nabahat Benmanssour ◽

M. Carmen González-Mas ◽

...

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Inhibitory Concentration ◽

Antibacterial Effect ◽

Biological Activities ◽

Inhibition Zone ◽

Bacterial Strains ◽

Gram Positive Bacteria ◽

Therapeutic Properties

The plants of the Apiaceae family are mainly used for food purposes and their multiple therapeutic properties and biological activities. This study aims to determine the chemical composition of Essential Oil (EO) of the fruits of Smyrnium olusatrum L. obtained by hydrodistillation in Clevenger-type apparatus. GC/MS analyzed the EO and the antibacterial activity was evaluated against clinical bacterial strains by two complementary methods (Disc diffusion and micro-atmosphere) and determination of Minimal Inhibitory Concentration (MIC). The results of chemical composition show that the EO is dominated by hydrocarbon monoterpenes (55,95%). The antibacterial effect shows that Gram-positive bacteria are the most sensitive to the inhibitory action of EO with inhibition zone diameters (DZI) ranging from 18 to 20 mm for Staphylococcus aureus and Streptococcus faecium strains. The same results were reported with the micro-atmosphere

Download Full-text

Chemical Composition, Antibacterial, Insecticidal and Anti-Oxidant Activities of Three Acantholimon Species (A. atropatanum, A. gilliatii and A. tragacanthium)

The Natural Products Journal ◽

10.2174/2210315509666190117153456 ◽

2020 ◽

Vol 10 (3) ◽

pp. 272-278

Author(s):

Ardalan Pasdaran ◽

Satyajit D. Sarker ◽

Lutfun Nahar ◽

Azadeh Hamedi

Keyword(s):

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Main Compound ◽

Aerial Parts ◽

Anti Oxidant ◽

Gas Chromatography Mass Spectroscopy

Background: The essential oil from the Acantholimon genus have been an integral part of the traditional food additive in Middle East. Most of the plants in Acantholimon genus have not been studied scientifically. The aim of this study is to investigate the chemical composition, antibacterial, insecticidal and anti-oxidant activities of three Acantholimon species including Acantholimon atropatanum, A. gilliatii and A. tragacanthium. Method: The essential oils of the aerial parts were extracted by hydrodistillation. Chemical constitutions were identified by gas chromatography- mass spectroscopy technique, also their toxicities were assessed against the two important grain products pests, Oryzeaphilus mercator and Tribolium castaneum. Antibacterial activity was assessed against the three foodborne bacteria that include Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus based on the disc diffusion assay. Free-radical-scavenging property was identified based on 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. Results: 2-hexahydrofarnesyl acetone was the main compound in A. gilliatii and A. tragacanthium, whilst farnesyl acetone, heptacosane and germacrene D were the principal components of A. atropatanum essential oil. These oils exhibited 40-90% mortality of O. mercator and/or T. castaneum at a dose of 12 μl/l air after 48h of exposure, and exhibited significant free-radicalscavenging property (RC50 = 3.7 × 10-3 - 8.3 × 10-3 mg/ml). The oils of A. tragacanthium and A. gilliatii showed a weaker antibacterial activity compared to A. atropatanum. Conclusion: A. atropatanum, A. gilliatii and A. tragacanthium essential oils had significant insecticidal and anti-oxidant properties. They also showed week to moderate antibacterial activity against P. aeruginosa and S. aureus.

Download Full-text

Chemical Constituents of the Essential Oil from Ecuadorian Endemic Species Croton ferrugineus and Its Antimicrobial, Antioxidant and α-Glucosidase Inhibitory Activity

Molecules ◽

10.3390/molecules26154608 ◽

2021 ◽

Vol 26 (15) ◽

pp. 4608

Author(s):

Eduardo Valarezo ◽

Génesis Gaona-Granda ◽

Vladimir Morocho ◽

Luis Cartuche ◽

James Calva ◽

...

Keyword(s):

Gas Chromatography ◽

Essential Oil ◽

Endemic Species ◽

Chemical Constituents ◽

Radical Scavenging ◽

Moderate Activity ◽

Gram Positive Bacteria ◽

Flame Ionization ◽

Total Composition ◽

Radical Scavenging Properties

Croton ferrugineus Kunth is an endemic species of Ecuador used in traditional medicine both for wound healing and as an antiseptic. In this study, fresh Croton ferrugineus leaves were collected and subjected to hydrodistillation for extraction of the essential oil. The chemical composition of the essential oil was determined by gas chromatography equipped with a flame ionization detector and gas chromatography coupled to a mass spectrometer using a non-polar and a polar chromatographic column. The antibacterial activity was assayed against three Gram-positive bacteria, one Gram-negative bacterium and one dermatophyte fungus. The radical scavenging properties of the essential oil was evaluated by means of DPPH and ABTS assays. The chemical analysis allowed us to identify thirty-five compounds representing more than 99.95% of the total composition. Aliphatic sesquiterpene hydrocarbon trans-caryophyllene was the main constituent with 20.47 ± 1.25%. Other main compounds were myrcene (11.47 ± 1.56%), β-phellandrene (10.55 ± 0.02%), germacrene D (7.60 ± 0.60%), and α-humulene (5.49 ± 0.38%). The essential oil from Croton ferrugineus presented moderate activity against Candida albicans (ATCC 10231) with an MIC of 1000 μg/mL, a scavenging capacity SC50 of 901 ± 20 µg/mL with the ABTS method, and very strong antiglucosidase activity with an IC50 of 146 ± 20 µg/mL.

Download Full-text