scholarly journals Chemical Composition, Antioxidant and Antibacterial Properties of Essential Oil from Ocotea Auriculiformis Kost. (Lauraceae) Leaves, Endemic of Madagascar

2017 ◽  
Vol 13 (33) ◽  
pp. 364 ◽  
Author(s):  
Andrianantenaina Rigobert ◽  
Ralambondrahety Rahanira ◽  
Andriambeloson Onja ◽  
Rabehaja Delphin ◽  
Ralamboranto Laurence ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Chemical Composition ◽  
Essential Oil ◽  
Bacillus Cereus ◽  
Antioxidant Properties ◽  
Antibacterial Properties ◽  
Quantitative Measure ◽  
Inhibition Zone ◽  
Endemic Plant

Plants constitute an important source of secondary metabolites in which essential oils are well-known for their use in various domains such as pharmacy, therapeutic, cosmetology and foods. In vitro antimicrobial and antioxidant properties of Ocotea auriculiformis Kost. (Lauraceae) leaves essential oil is demonstrated and its chemical composition is reported in the present study. The essential oil from Ocotea auriculiformis Kost. (Lauraceae) leaves, an endemic plant of Madagascar was extracted by hydrodistillation method. Chemical composition using GC, GC/ MS and NMR13C methods showed that the essential oil contained around 47 products in which 93.95% were identified. Known compounds are constituted by 74.7% of hydrocarbons and 19.25% of oxygenated products. The essential oil is rich in sesquiterpene and monoterpene. In vitro antibacterial capacity of the essential oil was assessed by disc method against human and food pathogens. Bacillus cereus and Streptococcus pneumoniae were very sensitive to the essential oil with 11 mm and 25 mm of inhibition zone respectively. The MIC of the essential oil was 1mg/mL for Bacillus cereus and 0.25 mg/mL for Streptococcus pneumoniae. MBC values were 2.5 mg/mL and 0.5 mg/mL, respectively. The ratio MBC/MIC for both strains was inferior to 4 concluding hence that the essential oil has bactericidal effect against the two sensitive strains. In vitro antioxidant capacity of the essential oil was performed according to qualitalive (TLC) and quantitative (measure of DPPH radical scavengening) methods. The essential oil showed antioxidant activity with IC50 value of 0.35 mg/mL.

scholarly journals Chemical Composition, In Vitro and In Silico Antioxidant Potential of Melissa officinalis subsp. officinalis Essential Oil

Antioxidants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1081
Author(s):  
Matilda Rădulescu ◽  
Călin Jianu ◽  
Alexandra Teodora Lukinich-Gruia ◽  
Marius Mioc ◽  
Alexandra Mioc ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
In Silico ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Inhibitory Effect ◽  
Melissa Officinalis ◽  
Β Carotene

The investigation aimed to study the in vitro and in silico antioxidant properties of Melissa officinalis subsp. officinalis essential oil (MOEO). The chemical composition of MOEO was determined using GC–MS analysis. Among 36 compounds identified in MOEO, the main were beta-cubebene (27.66%), beta-caryophyllene (27.41%), alpha-cadinene (4.72%), caryophyllene oxide (4.09%), and alpha-cadinol (4.07%), respectively. In vitro antioxidant properties of MOEO have been studied in 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging, and inhibition of β-carotene bleaching assays. The half-maximal inhibitory concentration (IC50) for the radical scavenging abilities of ABTS and DPPH were 1.225 ± 0.011 μg/mL and 14.015 ± 0.027 μg/mL, respectively, demonstrating good antioxidant activity. Moreover, MOEO exhibited a strong inhibitory effect (94.031 ± 0.082%) in the β-carotene bleaching assay by neutralizing hydroperoxides, responsible for the oxidation of highly unsaturated β-carotene. Furthermore, molecular docking showed that the MOEO components could exert an in vitro antioxidant activity through xanthine oxidoreductase inhibition. The most active structures are minor MOEO components (approximately 6%), among which the highest affinity for the target protein belongs to carvacrol.

scholarly journals Chemical Composition of Essential Oil from Flower Heads of Arnica Chamissonis Less. under a Nitrogen Impact

Molecules ◽  
2019 ◽  
Vol 24 (24) ◽  
pp. 4454
Author(s):  
Danuta Sugier ◽  
Katarzyna Olesińska ◽  
Piotr Sugier ◽  
Małgorzata Wójcik
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Plant Species ◽  
Antioxidant Properties ◽  
Nitrogen Application ◽  
Endemic Plant ◽  
Alpha Pinene ◽  
Full Flowering ◽  
Germacrene D ◽  
Pharmacologically Active

Chamisso arnica (Arnica chamissonis Less.) is a valuable plant species used in the pharmaceutical industry due to the content of many pharmacologically active substances and the similarity of its chemical composition to that of Arnica montana—a medicinal plant commonly used in pharmacy and cosmetics. The similarity of the two plant species implies that chamisso arnica can be a pharmaceutical substitute for the mountain arnica, i.e., an endangered and endemic plant species in Europe. Chamisso arnica extracts exhibit anti-inflammatory and antiradical activity and possesses high antioxidant properties that might be helpful in preventing or delaying the progress of free radical dependent diseases. The attributes of A. chamissonis are mainly related to the content and chemical composition of essential oil. Therefore, the objective of this study was to characterize the chemical composition of essential oil derived from A. chamissonis flower heads under a nitrogen impact. The experiment was performed on experimental fields in mid-eastern Poland on two soil types (sandy and loamy soils). The nitrogen fertilizer was applied as ammonium sulfate (control, 30, 60, 90, and 120 kg N ha−1). Collection of flower heads was carried out in the full flowering phase, which was characterized by the highest content of essential oil. The chemical composition of essential oil was examined using GC-MS. Among the 75 ingredients of the volatile oil of chamisso arnica flower heads, alpha-pinene, cumene, p-cymene, germacrene D, spathulenol, decanal, caryophyllene oxide, beta-pinene, and benzene acetaldehyde were present at relatively high levels. Both the nitrogen application and the soil type had an effect on the oil concentration and the yield of the main constituents (alpha-pinene and germacrene D) with pharmacological value. Different levels of nitrogen application could be considered as a relevant way to modify the chemical composition and to increase the essential oil production.

scholarly journals Chemical Composition, Antioxidant Properties and Anti-cholinesterase Activity of Cordia gilletii (Boraginaceae) Leaves Essential Oil

2011 ◽  
Vol 6 (2) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Marco Bonesi ◽  
Philippe N. Okusa ◽  
Rosa Tundis ◽  
Monica R. Loizzo ◽  
Federica Menichini ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Antioxidant Properties ◽  
In Vitro Tests ◽  
Bleaching Test ◽  
Total Oil ◽  
Β Carotene ◽  
Terpene Derivatives

This study aimed to investigate for the first time the chemical composition, the antioxidant properties and the acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activity of the essential oil from the leaves of Cordia gilletii De Wild (Boraginaceae). The essential oil, characterized by 23 constituents (90.1% of the total oil), was constituted by terpene derivatives (25.6%) and non-terpene derivatives (64.5%), among which aldehydes, fatty acids and alkanes were present with the percentage of 16.5%, 18.8% and 23.1%, respectively. The antioxidant activity of C. gilletii essential oil was screened by two in vitro tests: DPPH and β-carotene bleaching test. The essential oil revealed antioxidant activity with an IC50 value of 75.0 and 129.9 μg/mL on DPPH radical and β-carotene decoloration tests, respectively. Moreover, C. gilletii inhibited AChE enzyme with an IC50 value of 105.6 μg/mL.

Chemical Composition, Antifungal and In Vitro Antioxidant Properties ofMonarda didymaL. Essential Oil

2006 ◽  
Vol 18 (5) ◽  
pp. 581-585 ◽  
Author(s):  
Daniele Fraternale ◽  
Laura Giamperi ◽  
Anahi Bucchini ◽  
Donata Ricci ◽  
Francesco Epifano ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Antioxidant Properties

scholarly journals Chemical Composition, Antibacterial, Enzyme-Inhibitory, and Anti-Inflammatory Activities of Essential Oil from Hedychiumpuerense Rhizome

Agronomy ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2506
Author(s):  
Yi Hong ◽  
Xiongli Liu ◽  
Huijuan Wang ◽  
Min Zhang ◽  
Minyi Tian
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Biological Activities ◽  
Antibacterial Properties ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Minimal Bactericidal Concentration ◽  
Edible Plant ◽  
Anti Inflammatory ◽  
Factor Α

Hedychium puerense, a perennial rhizomatous herb, is used as an ornamental, medicinal, and edible plant in Yunnan Province, China. Essential oils from Hedychium plants are widely used in perfumes and traditional medicine, but there are no studies on the constituents and bioactivities of H. puerense essential oil (EO). Therefore, this study was designed to explore the chemical composition, antibacterial, enzyme-inhibitory, and anti-inflammatory activities of H. puerense rhizome EO. The gas chromatography with flame ionization or mass selective detection (GC-FID/MS) results indicated that H. puerense EO was mainly composed of linalool (26.5%), β-pinene (18.6%), γ-terpinene (12.1%), terpinen-4-ol (7.7%), α-pinene (5.8%), sabinene (4.9%), E-nerolidol (4.1%), and p-cymene (3.6%). For biological activities, H. puerense EO displayed broad-spectrum antibacterial properties against Enterococcus faecalis, Bacillus subtilis, Staphylococcus aureus, Proteus vulgaris, Pseudomonas aeruginosa, and Escherichia coli with diameter of inhibition zone (DIZ) values ranging from 7.44 to 10.30 mm, a minimal inhibitory concentration (MIC) of 3.13–6.25 mg/m), and a minimal bactericidal concentration (MBC) of 3.13–12.50 mg/mL. Moreover, the EO significantly inhibited acetylcholinesterase (AChE) (IC50 = 0.94 ± 0.02 mg/mL) and butyrylcholinesterase (BChE) (IC50 = 1.32 ± 0.06 mg/mL) activities, and exhibited a moderate inhibitory effect on α-glucosidase (IC50 = 5.42 ± 0.32 mg/mL) and tyrosinase (IC50 = 3.23 ± 0.21 mg/mL). Furthermore, the EO significantly suppressed the secretion of the pro-inflammatory mediator, nitric oxide (NO) (99.23 ± 0.26%), cytokines tumor necrosis factor-α (TNF-α) (97.14 ± 0.11%), and interleukin-6 (IL-6) (82.42 ± 0.16%) in lipopolysaccharide (LPS)-stimulated RAW264.7 cells at 250 μg/mL without cytotoxicity. Hence, H. puerense EO can be considered a bioactive, natural product that has great potential for utilization in the fields of food, cosmetics, and pharmaceutics.

scholarly journals Antibacterial and Antioxidant Properties of the Leaves and Stem Essential Oils ofJatropha gossypifoliaL.

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Sunday O. Okoh ◽  
Benson C. Iweriebor ◽  
Omobola O. Okoh ◽  
Uchechukwu U. Nwodo ◽  
Anthony I. Okoh
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Antioxidant Properties ◽  
Antibacterial Properties ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Inhibition Concentration ◽  
Jatropha Gossypifolia ◽  
Leaf Essential Oil

Antibacterial and antioxidant properties of the leaves and stem essential oils (EOs) ofJatropha gossypifoliaand their efficacies against infectious and oxidative stress diseases were studiedin vitro. The EOs obtained using Clevenger modified apparatus were characterized by high resolution GC-MS, while their antioxidant and antibacterial properties were examined by spectrophotometric and agar diffusion techniques, respectively. The EOs exhibited strong antibacterial activity againstEscherichia coli,Enterococcus faecium,andStaphylococcus aureus. The stem essential oil (SEO) was more active than the leaf essential oil (LEO) against test bacteria with minimum inhibition concentration (MIC) ranging from 0.025 to 0.05 mg/mL and the LEO from 0.05 to 0.10 mg/mL. The SEO was bactericidal at 0.025 and 0.05 mg/mL againstS.aureusandE.faecium, respectively, and the LEO was bacteriostatic against the three bacteria at 0.05 and 0.10 mg/mL. The SEO IC50(0.07 mg/mL) showed that the antiradical strength was superior to LEO (0.32 mg/mL) andβ-carotene (1.62 mg/mL) in scavenging 2, 2-diphenyl-1-picrylhydrazyl radicals (DPPH•). The oils effectively reduced three other oxidants to neutral molecules in concentration dependent manner. Findings from this study suggest that, apart from the traditional uses of the plant extracts, the EOs have strong bioactive compounds with noteworthy antibacterial and antiradical properties and may be good candidates in the search for lead compounds for the synthesis of novel potent antibiotics.

scholarly journals Chemical Composition, Antioxidant and Antimicrobial Activity of Raspberry, Blackberry and Raspberry-Blackberry Hybrid Leaf Buds

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 327
Author(s):  
Anna Krzepiłko ◽  
Roman Prażak ◽  
Agata Święciło
Keyword(s):  
Chemical Composition ◽  
Growth Parameters ◽  
Antioxidant Properties ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Enterobacter Aerogenes ◽  
Total Phenolic ◽  
Glycerol Water ◽  
Hypertonic Medium

In our investigation, the chemical composition and bioactive potential of leaf buds of raspberry, blackberry, and a raspberry-blackberry hybrid were determined. Antioxidant and antimicrobial properties were tested in water (W), ethanol-water (EW), and glycerol-water (GW) extracts from the buds. These plant organs contain relatively large amounts of minerals, especially Fe. The total antioxidant capacity (TAC) measured by the ABTS and DPPH methods ranged from 2.86 to 12.19 and 6.75 to 24.26 mmol per 100 g fresh weight (FW) of buds, respectively. TAC values were generally higher in the raspberry than in the case of blackberry and raspberry-blackberry hybrid extracts. The antioxidant properties of the extracts were strongly positively correlated with their content of total phenolic (TP). No such relationship was noted for ascorbic acid (AA), whose concentration in all extracts was at a similarly low level. Antioxidant properties determined in vitro were confirmed for the GW extract from raspberry leaf buds in biological test based on the growth parameters of Δsod1Saccharomyces cerevisiae mutant cells in hypertonic medium. The extracts also exhibited strong antibacterial properties against Staphylococcus aureus and Enterococcus faecalis and weaker against Enterobacter aerogenes. The studied leaf buds could be therefore an unconventional source of minerals, natural antioxidants and antibacterial compounds with potential applications in the food, pharmaceutical, and cosmetics industries.

scholarly journals In Vitro Effect of Plant Parts Extract of Senecio glaucus L. on Pathogenic Bacteria

2021 ◽  
Vol 12 (3) ◽  
pp. 3800-3810
Keyword(s):  
Chemical Composition ◽  
Pathogenic Bacteria ◽  
Antioxidant Properties ◽  
Inhibition Zone ◽  
Antimicrobial Activities ◽  
Bioactive Constituents ◽  
Two Samples ◽  
Ic50 Values ◽  
Vitro Effect

Senecio glaucus L. is an annual herb that grows in several Egyptian desert habitats. The diversity of habitats inhabited by this species, as well as its distribution, chemical composition, and biological activity, are all unknown. This research aimed to examine the chemical composition of S. glaucus from various environments in Egypt, as well as the antioxidant and antimicrobial activities. The general assessment of the analytical results for different parts of S. glaucus showed that the capitula and leaves in both inland and coastal samples were rich in bioactive constituents than the other parts as following (capitula > leaf > root > stem). Based on the results of IC50, the antioxidant properties of the eight parts of two samples follows the sequence capitula ˃ root ˃ leaf ˃ stem for the coastal sample, and capitula ˃ leaf ˃ stem ˃ root for the inland sample. The IC50 values ranged from 25.94 to 41.20 mg/ml in coastal sample, where the IC50 values ranged from 28.02 to 42.83 mg/ml in desert sample, compared to ascorbic acid (IC50 = 13.30 mg/ml). The antimicrobial potential of MeOH extracts of S. glaucus parts collected from different habitats exhibited different inhibitory spectrum behavior with varying degrees of inhibition against six Gram-positive bacteria and four Gram-negative bacteria. In both coastal and inland samples, the E. coli inhibition zone was the most susceptible bacterium. Whereas, in the case of the coastal sample, the inhibition zone of B. subtilis was the most sensitive bacterium. The results of the antibacterial test were compared with 3 standard antibiotics.

scholarly journals GC/MS Evaluation and In Vitro Antioxidant Activity of Essential Oil and Solvent Extracts of an Endemic Plant Used as Folk Remedy in Turkey:Phlomis bourgaeiBoiss.

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Cengiz Sarikurkcu ◽  
M. Sabih Ozer ◽  
Ahmet Cakir ◽  
Mustafa Eskici ◽  
Ebru Mete
Keyword(s):  
Linoleic Acid ◽  
Chemical Composition ◽  
Essential Oil ◽  
Water Extract ◽  
Reducing Power ◽  
Endemic Plant ◽  
Folk Remedy ◽  
Excellent Activity ◽  
Chelating Effect

This study was outlined to examine the chemical composition of hydrodistilled essential oil and in vitro antioxidant potentials of the essential oil and different solvent extracts of endemicPhlomis bourgaeiBoiss. used as folk remedy in Turkey. The chemical composition of the oil was analyzed by GC and GC-MS, and the predominant components in the oil were found to beβ-caryophyllene (37.37%),(Z)-β-farnesene (15.88%), and germacrene D (10.97%). Antioxidant potentials of the solvent extracts and the oil were determined by four testing systems includingβ-carotene/linoleic acid, DPPH, reducing power, and chelating effect. Inβ-carotene/linoleic acid assay, all extracts showed the inhibition of more than 50% at all concentrations. In DPPH, chelating effect, and reducing power test systems, the water extract with 88.68%, 77.45%, and 1.857 (absorbance at 700 nm), respectively, exhibited more excellent activity potential than other extracts (hexane, ethyl acetate and methanol) and the essential oil at 1.0 mg/mL concentration. The amount of the total phenolics and flavonoids was the highest in this extract (139.50 ± 3.98 μg gallic acid equivalents (GAEs)/mg extract and 22.71 ± 0.05μg quercetin equivalents (QEs)/mg extract).

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