GC/MS Evaluation and In Vitro Antioxidant Activity of Essential Oil and Solvent Extracts of an Endemic Plant Used as Folk Remedy in Turkey:Phlomis bourgaeiBoiss.

This study was outlined to examine the chemical composition of hydrodistilled essential oil and in vitro antioxidant potentials of the essential oil and different solvent extracts of endemicPhlomis bourgaeiBoiss. used as folk remedy in Turkey. The chemical composition of the oil was analyzed by GC and GC-MS, and the predominant components in the oil were found to beβ-caryophyllene (37.37%),(Z)-β-farnesene (15.88%), and germacrene D (10.97%). Antioxidant potentials of the solvent extracts and the oil were determined by four testing systems includingβ-carotene/linoleic acid, DPPH, reducing power, and chelating effect. Inβ-carotene/linoleic acid assay, all extracts showed the inhibition of more than 50% at all concentrations. In DPPH, chelating effect, and reducing power test systems, the water extract with 88.68%, 77.45%, and 1.857 (absorbance at 700 nm), respectively, exhibited more excellent activity potential than other extracts (hexane, ethyl acetate and methanol) and the essential oil at 1.0 mg/mL concentration. The amount of the total phenolics and flavonoids was the highest in this extract (139.50 ± 3.98 μg gallic acid equivalents (GAEs)/mg extract and 22.71 ± 0.05μg quercetin equivalents (QEs)/mg extract).

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Chemical Composition, Antioxidant and Antibacterial Properties of Essential Oil from Ocotea Auriculiformis Kost. (Lauraceae) Leaves, Endemic of Madagascar

European Scientific Journal ESJ ◽

10.19044/esj.2017.v13n33p364 ◽

2017 ◽

Vol 13 (33) ◽

pp. 364 ◽

Cited By ~ 1

Author(s):

Andrianantenaina Rigobert ◽

Ralambondrahety Rahanira ◽

Andriambeloson Onja ◽

Rabehaja Delphin ◽

Ralamboranto Laurence ◽

...

Keyword(s):

Streptococcus Pneumoniae ◽

Chemical Composition ◽

Essential Oil ◽

Bacillus Cereus ◽

Antioxidant Properties ◽

Antibacterial Properties ◽

Quantitative Measure ◽

Inhibition Zone ◽

Endemic Plant

Plants constitute an important source of secondary metabolites in which essential oils are well-known for their use in various domains such as pharmacy, therapeutic, cosmetology and foods. In vitro antimicrobial and antioxidant properties of Ocotea auriculiformis Kost. (Lauraceae) leaves essential oil is demonstrated and its chemical composition is reported in the present study. The essential oil from Ocotea auriculiformis Kost. (Lauraceae) leaves, an endemic plant of Madagascar was extracted by hydrodistillation method. Chemical composition using GC, GC/ MS and NMR13C methods showed that the essential oil contained around 47 products in which 93.95% were identified. Known compounds are constituted by 74.7% of hydrocarbons and 19.25% of oxygenated products. The essential oil is rich in sesquiterpene and monoterpene. In vitro antibacterial capacity of the essential oil was assessed by disc method against human and food pathogens. Bacillus cereus and Streptococcus pneumoniae were very sensitive to the essential oil with 11 mm and 25 mm of inhibition zone respectively. The MIC of the essential oil was 1mg/mL for Bacillus cereus and 0.25 mg/mL for Streptococcus pneumoniae. MBC values were 2.5 mg/mL and 0.5 mg/mL, respectively. The ratio MBC/MIC for both strains was inferior to 4 concluding hence that the essential oil has bactericidal effect against the two sensitive strains. In vitro antioxidant capacity of the essential oil was performed according to qualitalive (TLC) and quantitative (measure of DPPH radical scavengening) methods. The essential oil showed antioxidant activity with IC50 value of 0.35 mg/mL.

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Chemical Characterization and In vitro Antimicrobial Activity of Caralluma europaea Essential Oil and Its Synergistic Potential with Conventional Antibiotics

Journal of Advances in Medical and Pharmaceutical Sciences ◽

10.9734/jamps/2018/v19i430094 ◽

2019 ◽

pp. 1-11 ◽

Cited By ~ 2

Author(s):

Loubna Ait Dra ◽

Abdellah Aghraz ◽

Brahim Boualy ◽

Saadia Oubaassine ◽

Mustapha Barakate ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Chemical Composition ◽

Essential Oil ◽

Aerial Part ◽

Reducing Power ◽

Antimicrobial Activities ◽

Acid Oxidation ◽

Pharmaceutical Industries

Aim: This study was carried out to investigate the antioxidant and antimicrobial activities of essential oil from the aerial part of Caralluma europaea and to evaluate the synergistic potential between essential oil and antibiotics. Methodology: The chemical composition, antioxidant, antimicrobial activities and synergetic interaction between antimicrobial agents and essential oil isolated by hydrodistillation from the aerial part of C. europaea were evaluated. The chemical composition was analyzed by a Gas chromatography/mass spectrometry (GC/MS) system. Antioxidant activity was measured employing three methods: scavenging of free radical DPPH, reducing power assay and the inhibition of linoleic acid oxidation. The antimicrobial activity of essential oil against microbial strains was qualitatively and quantitatively assessed by the presence or absence of inhibition zones diameters, and MIC values. The in vitro association between essential oil and some commercial antibiotics was also investigated. Results: The GC/MS analysis shows that a total of 21 constituents were identified and the main compounds were Terpinolene (19.5%), α-Terpinene (16.2%) and Linalool (15.3%). Antioxidant study showed that essential oil exhibited antioxidant activity with IC50 values ranging from 0.32 mg/ml to 1.45 mg/ml. The results of antimicrobial activity showed that the essential oil had an inhibitory effect against the majority of tested microorganisms except K. pneumonia and P. aeruginosa. Gram-positive bacteria were found to be more sensitive than Gram-negative ones. Furthermore, essential oil approved an interesting antifungal activity against yeast species. Out of 25 combinations tested 64% showed total synergism, 20% had a partial synergistic interaction and 16% showed no effect. The best synergistic effect was obtained with the combination essential oil-gentamycin. Conclusion: Our results are of a great importance and suggest that C. europaea essential oil contain bioactive compounds with antioxidant and antimicrobial properties with possible applications in the food and pharmaceutical industries.

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Chemical Composition, Antioxidant, Antimicrobial and Insecticidal Activities of Essential Oil from a Moroccan Endemic Plant: Bubonium imbricatum

Natural Product Communications ◽

10.1177/1934578x1601101123 ◽

2016 ◽

Vol 11 (11) ◽

pp. 1934578X1601101

Author(s):

Abdellah Aghraz ◽

Jürgen Wanner ◽

Erich Schmidt ◽

Loubna Aitdra ◽

Malika Aitsidibrahim ◽

...

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Reducing Power ◽

Inhibition Zone ◽

Moderate Activity ◽

Endemic Plant ◽

Strong Activity ◽

Power Test ◽

Gram Positive Bacteria ◽

Aerial Parts

The chemical composition, antioxidant, antimicrobial and insecticidal properties of essential oil obtained by hydro-distillation from Bubonium imbricatum Cav. have been investigated. The essential oil of aerial parts was analyzed by GC-FID and GC-MS. A total of 63 compounds were identified representing more than 82% of the oil. The major components were cis-chrysanthenyl acetate (31.2%) and thymol isobutyrate (3.4%). The antioxidant activity was evaluated by the DPPH and reducing power test. The data showed moderate activity compared with the reference compounds. Our results showed strong activity of the investigated oil against all tested microorganisms. The highest antibacterial activity was observed against Bacillus subtilis (inhibition zone= 18 ± 0.21, MIC= 0.18 mg/mL). In general, Gram-positive bacteria were more susceptible than Gram-negative ones. Candida parapsilosis was the most sensitive among the Candida strains tested. LD50 and LD90 values were 33.1 and 53.1 ppm against 1-d-old larvae of Aedes aegypti L.

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Helichrysum italicum (Roth) G. Don Essential Oil from Serbia: Chemical Composition, Classification and Biological Activity—May It Be a Suitable New Crop for Serbia?

Agronomy ◽

10.3390/agronomy11071282 ◽

2021 ◽

Vol 11 (7) ◽

pp. 1282

Author(s):

Milica Aćimović ◽

Jovana Ljujić ◽

Jelena Vulić ◽

Valtcho D. Zheljazkov ◽

Lato Pezo ◽

...

Keyword(s):

Biological Activity ◽

Chemical Composition ◽

Essential Oil ◽

Reducing Power ◽

Value Added ◽

Pharmaceutical Products ◽

Retention Indices ◽

Gas Chromatography Mass Spectrometry ◽

Bleaching Test

H. italicum essential oil (EO) is one of the most popular ingredients utilized by the cosmetic industry, and it is also used as natural antioxidant and as a value-added ingredient in food products. The chemical composition of the EO H. italicum cultivated in Serbia was analyzed by gas chromatography–mass spectrometry. The quantitative structure–retention relationship was used to predict the retention indices of the EO constituents acquired by GC-MS data, applying five molecular descriptors selected by factor analysis and a genetic algorithm. Also, antimicrobial activity, and biological activity by four common antioxidant tests (DPPH and ABTS assays, reducing power, and β-carotene bleaching test), and in vitro antihyperglycemic and anti-inflammatory capacities were evaluated. A total of 70 EO constituents were detected, of which 17 (8.5%) could not be identified. The H. italicum EO in this study belonged to γ-curcumene chemotype. The coefficients of determination reached the value of 0.964, demonstrating that this model could be used for prediction purposes. All applied tests showed that H. italicum EO possesses good biological activity and an interesting chemical composition. Therefore, the EO of H. italicum grown in Serbia has a potential to be used in food, cosmetic, and pharmaceutical products.

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Comparative Analysis of In-Vitro Biological Activities of Methyl Eugenol Rich Cymbopogon khasianus Hack., Leaf Essential Oil with Pure Methyl Eugenol Compound

Current Pharmaceutical Biotechnology ◽

10.2174/1389201021666200217113921 ◽

2020 ◽

Vol 21 (10) ◽

pp. 927-938 ◽

Cited By ~ 7

Author(s):

Roktim Gogoi ◽

Rikraj Loying ◽

Neelav Sarma ◽

Twahira Begum ◽

Sudin K. Pandey ◽

...

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Biological Activities ◽

Methyl Eugenol ◽

Dpph Assay ◽

Pure Compound ◽

Allium Cepa Assay ◽

Anti Inflammatory ◽

Lemongrass Essential Oil

Background: The essential oil of methyl eugenol rich Cymbopogon khasianus Hack. was evaluated and its bioactivities were compared with pure methyl eugenol. So far, methyl eugenol rich essential oil of lemongrass was not studied for any biological activities; hence, the present study was conducted. Objective: This study examined the chemical composition of essential oil of methyl eugenol rich Cymbopogon khasianus Hack., and evaluated its antioxidant, anti-inflammatory, antimicrobial, and herbicidal properties and genotoxicity, which were compared with pure compound, methyl eugenol. Material and Methods: Methyl eugenol rich variety of Cymbopogon khasianus Hack., with registration no. INGR18037 (c.v. Jor Lab L-9) was collected from experimental farm CSIR-NEIST, Jorhat, Assam (26.7378°N, 94.1570°E). The essential oil wasobtained by hydro-distillation using a Clevenger apparatus. The chemical composition of the essential oil was evaluated using GC/MS analysis and its antioxidant (DPPH assay, reducing power assay), anti-inflammatory (Egg albumin denaturation assay), and antimicrobial (Disc diffusion assay, MIC) properties, seed germination effect and genotoxicity (Allium cepa assay) were studied and compared with pure Methyl Eugenol compound (ME). Results: Major components detected in the Essential Oil (EO) through Gas chromatography/mass spectroscopy analysis were methyl eugenol (73.17%) and β-myrcene (8.58%). A total of 35components were detected with a total identified area percentage of 98.34%. DPPH assay revealed considerable antioxidant activity of methyl eugenol rich lemongrass essential oil (IC50= 2.263 μg/mL), which is lower than standard ascorbic acid (IC50 2.58 μg/mL), and higher than standard Methyl Eugenol (ME) (IC50 2.253 μg/mL). Methyl eugenol rich lemongrass EO showed IC50 38.00 μg/mL, ME 36.44 μg/mL, and sodium diclofenac 22.76 μg/mL, in in-vitro anti-inflammatory test. Moderate antimicrobial activity towards the 8 tested microbes was shown by methyl eugenol rich lemongrass essential oil whose effectiveness against the microbes was less as compared to pure ME standard. Seed germination assay further revealed the herbicidal properties of methyl eugenol rich essential oil. Moreover, Allium cepa assay revealed moderate genotoxicity of the essential oil. Conclusion: This paper compared the antioxidant, anti-inflammatory, antimicrobial, genotoxicity and herbicidal activities of methyl eugenol rich lemongrass with pure methyl eugenol. This methyl eugenol rich lemongrass variety can be used as an alternative of methyl eugenol pure compound. Hence, the essential oil of this variety has the potential of developing cost-effective, easily available antioxidative/ antimicrobial drugs but its use should be under the safety range of methyl eugenol and needs further clinical trials.

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Chemical Composition, In Vitro and In Silico Antioxidant Potential of Melissa officinalis subsp. officinalis Essential Oil

Antioxidants ◽

10.3390/antiox10071081 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1081

Author(s):

Matilda Rădulescu ◽

Călin Jianu ◽

Alexandra Teodora Lukinich-Gruia ◽

Marius Mioc ◽

Alexandra Mioc ◽

...

Keyword(s):

Antioxidant Activity ◽

Chemical Composition ◽

Essential Oil ◽

In Silico ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Inhibitory Effect ◽

Melissa Officinalis ◽

Β Carotene

The investigation aimed to study the in vitro and in silico antioxidant properties of Melissa officinalis subsp. officinalis essential oil (MOEO). The chemical composition of MOEO was determined using GC–MS analysis. Among 36 compounds identified in MOEO, the main were beta-cubebene (27.66%), beta-caryophyllene (27.41%), alpha-cadinene (4.72%), caryophyllene oxide (4.09%), and alpha-cadinol (4.07%), respectively. In vitro antioxidant properties of MOEO have been studied in 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging, and inhibition of β-carotene bleaching assays. The half-maximal inhibitory concentration (IC50) for the radical scavenging abilities of ABTS and DPPH were 1.225 ± 0.011 μg/mL and 14.015 ± 0.027 μg/mL, respectively, demonstrating good antioxidant activity. Moreover, MOEO exhibited a strong inhibitory effect (94.031 ± 0.082%) in the β-carotene bleaching assay by neutralizing hydroperoxides, responsible for the oxidation of highly unsaturated β-carotene. Furthermore, molecular docking showed that the MOEO components could exert an in vitro antioxidant activity through xanthine oxidoreductase inhibition. The most active structures are minor MOEO components (approximately 6%), among which the highest affinity for the target protein belongs to carvacrol.

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Chemical Composition, in vitro Trypanocidal and Antibacterial Activities of the Essential Oil from the Dried Leaves of Eugenia dysenterica DC from Brazil

Journal of Essential Oil Bearing Plants ◽

10.1080/0972060x.2019.1626293 ◽

2019 ◽

Vol 22 (2) ◽

pp. 347-355 ◽

Cited By ~ 4

Author(s):

Larissa Sousa Santos ◽

Cassia Cristina Fernandes Alves ◽

Elisângela Barbosa Borges Estevam ◽

Carlos Henrique Gomes Martins ◽

Thayná de Souza Silva ◽

...

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Antibacterial Activities ◽

Eugenia Dysenterica

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Chemical composition, in vitro antitumor and pro-oxidant activities of Glandora rosmarinifolia (Boraginaceae) essential oil

PLoS ONE ◽

10.1371/journal.pone.0196947 ◽

2018 ◽

Vol 13 (5) ◽

pp. e0196947 ◽

Cited By ~ 14

Author(s):

Paola Poma ◽

Manuela Labbozzetta ◽

Monica Notarbartolo ◽

Maurizio Bruno ◽

Antonella Maggio ◽

...

Keyword(s):

Chemical Composition ◽

Essential Oil

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Phaseolus vulgaris L. var. Venanzio Grown in Tuscany: Chemical Composition and In Vitro Investigation of Potential Effects on Colorectal Cancer

Antioxidants ◽

10.3390/antiox9121181 ◽

2020 ◽

Vol 9 (12) ◽

pp. 1181

Author(s):

Federica Finetti ◽

Marco Biagi ◽

Jasmine Ercoli ◽

Giulia Macrì ◽

Elisabetta Miraldi ◽

...

Keyword(s):

Chemical Composition ◽

Phaseolus Vulgaris ◽

Unsaturated Fatty Acids ◽

Biological Activities ◽

Water Extract ◽

Phaseolus Vulgaris L ◽

Cellular Models ◽

In Vitro Investigation ◽

Promising Source

Phaseolus vulgaris L. (common bean) is a leguminous species that is an important dietary component due to its high content of proteins, unsaturated fatty acids, minerals, dietary fibers and vitamins. Due to the high content of polyphenols, several biological activities have been described for bean extracts, making it possible to include P. vulgaris among food with beneficial effects for human health. Moreover, more than 40,000 varieties of beans have been recognised with different nutraceutical properties, pointing out the importance of food biodiversity. In this work, we describe for the first time the chemical composition and biological activity of a newly recognized Italian variety of P. vulgaris grown in a restricted area of the Tuscany region and named “Fagiola di Venanzio”. Fagiola di Venanzio water extract is rich in proteins, sugars and polyphenols and displays antioxidant, anti-inflammatory and antiproliferative activities in in vitro assays on colon cancer cellular models. Our data indicate that this variety of P. vulgaris appears to be a promising source of bioactive compounds and encourage more in-depth studies to better elucidate the implications of its consumption for public health.

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ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF STEM BARK ESSENTIAL OIL CONSTITUENTS OF LITSEA GLUTINOSA C. B. ROB.

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2016v8i12.13577 ◽

2016 ◽

Vol 8 (12) ◽

pp. 258

Author(s):

Arunodaya H. S. ◽

Krishna V. ◽

Shashikumar R. ◽

Girish Kumar K.

Keyword(s):

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Stem Bark ◽

Diffusion Method ◽

Vibrio Cholera ◽

Litsea Glutinosa ◽

Β Carotene ◽

Mic Value

Objective: To evaluate the chemical composition, antibacterial and antioxidant properties of stem bark essential oil of Litsea glutinosa C. B. Rob.Methods: The essential oil isolated from stem bark of L. glutinosa and their chemical composition was analyzed by gas chromatography coupled with mass spectrometry detector. The in vitro antibacterial activity of the stem bark essential oil was investigated against eight human pathogenic bacterial clinical isolates using agar disc diffusion method and MIC value was determined by modified resazurin microtitre-plate assay. The antioxidant activity of essential oil was measured by 2, 2-diphenyl-1-picrylhydrazyl radical (DPPH), 2, 2-azinobis-3-ethylbenzothiazoline-6-sulphonate radical cation (ABTS) and β-carotene bleaching assay.Results: GC-MS analysis of stem bark essential oil resulted in the identification of 37 compounds, off which 9,12-octadecadienoic acid (62.57%), hexadecanoic acid (12.68%), stigmast-5-en-3-ol (6.87%) and vitamin E (2.51%) were the main constituents representing 84.63% of the oil. The determination of in vitro antibacterial activity of stem bark essential oil resulted in significant inhibition zone (15.00±0.57 mm) and MIC value (0.15±0.15×10-2 mg/ml) against the pathogenic bacteria Vibrio cholera followed by Pseudomonas aeruginosa and Salmonella typhi. The results of DPPH radical scavenging (IC50:4.540±0.06 µg/ml), ABTS (IC50:256.02±0.06 µg/ml) and β-carotene bleaching assay (%I: 78.51±0.42 %) showed significant in vitro antioxidant property.Conclusion: L. glutinosa stem bark essential oil showed potential antibacterial activity against the Vibrio cholera. The results of this investigation supported the ethnomedical claim of essential oil as a demulcent, antidiarrheal and antioxidant drug.

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