scholarly journals Preparation and Validated Analysis of Anthocyanin Concentrate from the Calyces of Hibiscus sabdariffa

2017 ◽  
Vol 12 (1) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Lubomir Opletal ◽  
Lucie Chocholousova-Havlikova ◽  
Tomas Siatka ◽  
Lucie Cahliková ◽  
Miroslav Locarek ◽  
...  
Keyword(s):  
Analytical Method ◽  
Anthocyanin Content ◽  
Hibiscus Sabdariffa ◽  
Chromatographic Purification ◽  
Purification Step ◽  
Extraction And Purification ◽  
Medicinal Properties ◽  
The Individual ◽  
Method Of Extraction ◽  
Column Chromatographic

Extracts of Hibiscus sabdariffa calyces have been shown to have various medicinal properties, some of which have been reported to be due to anthocyanins present in the calyces. To study whether these claims are valid, it is necessary to produce an extract with a high anthocyanin content and to have available a method to identify and quantify the individual compounds in the product. A method of extraction and purification has been developed based on a polyamide column chromatographic purification step. Using this method, anthocyanin concentrates were produced containing from 57 to 64% of delphinidin-3-sambubioside plus cyanidin-3-sambubioside. A rapid, efficient and validated HPLC analytical method was developed and used for the analysis of the anthocyanin concentrate.

AN IMPROVED METHOD FOR THE ASSAY OF PROGESTERONE BY COMPETITIVE PROTEIN BINDING

1970 ◽  
Vol 63 (2) ◽  
pp. 225-241 ◽  
Author(s):  
B. D. Reeves ◽  
M. L. A. de Souza ◽  
I. E. Thompson ◽  
E. Diczfalusy
Keyword(s):  
Protein Binding ◽  
Binding Sites ◽  
Entire Range ◽  
Improved Method ◽  
Plasma Progesterone ◽  
Extraction And Purification ◽  
Corticosteroid Binding Globulin ◽  
Competitive Protein Binding ◽  
The Individual ◽  
Range Of Values

ABSTRACT An improved method for the assay of plasma progesterone by competitive protein binding is described. The improvement is based upon rigorous control of the variables, the compensation for and standardisation of interfering factors inherent in the method and the use of a human corticosteroid binding globulin, that meets the requirements for sensitivity at levels of 1.0 ng of progesterone and below. The assessment of the reliability of the individual steps in the method as well as that of the complete method is presented. The sensitivity of the method is around 0.2 ng progesterone per ml plasma. Accuracy was measured by adding progesterone in amounts ranging from 0.0 to 1.0 ng to 1.0 ml plasma. There was a linear relationship between the progesterone added and recovered throughout the entire range of values, with a coefficient of correlation (r) of 0.94. Of 52 related steroids tested, none was found which would remain associated with progesterone following extraction and purification and which would also compete with progesterone for binding sites.

scholarly journals Differences in Anthocyanin Accumulation Profiles between Teinturier and Non-Teinturier Cultivars during Ripening

Foods ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1073
Author(s):  
Meng-Bo Tian ◽  
Lin Yuan ◽  
Ming-Yuan Zheng ◽  
Zhu-Mei Xi
Keyword(s):  
Gene Expression ◽  
Plant Secondary Metabolites ◽  
Anthocyanin Synthesis ◽  
Anthocyanin Content ◽  
Anthocyanin Accumulation ◽  
Total Anthocyanin ◽  
Total Anthocyanin Content ◽  
The Individual ◽  
Relative Gene ◽  
High Expression Level

Anthocyanins are vital components of plant secondary metabolites, and are also the most important coloring substances in wine. Teinturier cultivars are rich in anthocyanins. However, the differences in anthocyanin accumulation and profiles between teinturier and non-teinturier cultivars have not been reported. In this study, Yan 73 and Dunkelfelder were selected as the experimental materials, and three non-teinturier cultivars were used for comparison. LC-MS and qRT-PCR were used to determine the individual anthocyanin contents and the relative gene expression. The results show that the total anthocyanin content of the teinturier cultivars was considerably higher than that in non-teinturier cultivars, and the levels of individual anthocyanins increased gradually during ripening. Lower ratios of modified anthocyanins were found in the teinturier cultivars, which was not only due to the high expression level of VvUFGT and VvGST4, but also due to the relatively low expression of VvOMT in these cultivars. Cluster analysis of gene expression and anthocyanin accumulation showed that VvUFGT is related to anthocyanin accumulation, and that AM1 is related to the synthesis and transport of methylated anthocyanins. Our results will be useful for further clarifying the pathways of anthocyanin synthesis, modification, and transport in teinturier cultivars.

scholarly journals Phytochemical Composition of an Underutilized Plant Sorrel/Roselle (Hibiscus Sabdariffa L.) Cultivated in India

2020 ◽  
Vol 10 (2) ◽  
pp. 2138-2147
Keyword(s):  
Nutritional Composition ◽  
Total Phenolic ◽  
Hibiscus Sabdariffa ◽  
Bioactive Components ◽  
Therapeutic Value ◽  
Ethnic Food ◽  
Total Anthocyanins ◽  
Uv Visible ◽  
The Individual ◽  
Effective Component

Roselle (Hibiscus sabdariffa L) belongs to the Malvaceae family is a multipurpose plant with high economic importance and worldwide fame for its nutritional and therapeutic value, but unfortunately, it is underutilized in India. Their calyces are utilized as ethnic food and in traditional medicine to cure many degenerative diseases in different parts of the world and by the tribal of India. However, no evidence is available in the literature regarding the existent quality assessment of the roselle calyces in India. Therefore, the objective of the study was to characterize the calyces of roselle (Hibiscus sabdariffa L.) from India. The calyces were grounded, sieved to 20 mesh sizes, and stored in the refrigerator for further analysis. The parameters analyzed included nutritional composition and the biologically effective component that is total phenols and the total anthocyanins. A procedure to measure the anthocyanins by pH-differential UV-visible spectrometry was followed. Total phenolic content was assessed by the Folin Ciocalteau method, and gallic acid was used as a standard. The individual anthocyanins present in Hibiscus were characterized by TLC. The results reveal that these indigenous powdered calyces are rich sources of macro and micronutrients, bioactive components like polyphenols and flavonoids, especially anthocyanins. Our study substantiates the health /nutritional claims and validates its applicability in functional food processing and therapeutic treatments.

Low dose irradiation of plum peel anthocyanins: Impact on stability and antioxidant activity

2021 ◽  
Author(s):  
Peerzada R. Hussain ◽  
Prashant P. Suradkar
Keyword(s):  
Antioxidant Activity ◽  
Low Dose ◽  
B Value ◽  
Natural Food ◽  
Thermal Methods ◽  
Dose Irradiation ◽  
Extraction And Purification ◽  
Irradiation Treatment ◽  
The Individual ◽  
Anthocyanin Degradation

Consumer demand for both natural food colorants and non-thermal methods of preservation has increased overwhelmingly during the last few decades. Preventing loss of appeal and color through non-thermal methods without compromising on quality will provide an alternative to conventional thermal methods. In the present study, irradiation of plum peel anthocyanins after extraction and purification was done in a range of 0.1-1.0 kGy. Results revealed that degradation was significantly (p ≤ 0.05) dependent on both time and dose of irradiation. The percentage degradation observed in the first 3 h of treatment was 9.0-21.0±1.2%. The maximum anthocyanin degradation recorded during 8 h of irradiation was 45.3%. Anthocyanin degradation was apparently less at 0.1-0.5 kGy compared to 0.6-1.0 kGy. The retention of anthocyanins was up to the extent of 88.8-62.3% in samples irradiated at 0.1-0.5 kGy. Comparison of the individual anthocyanin indicated that diglucoside anthocyanins were stable towards irradiation than monoglucoside anthocyanins. For monoglucoside anthocyanins, a 50% decrease was observed as the irradiation treatment exceeded 0.6 kGy. A close comparison of the data revealed that at irradiation dose of 1.0 kGy, the decrease in L* and a* values was almost four and three times higher than that observed in samples irradiated at 0.5 kGy and below; while an increase in b* value was almost double. In spite of marginal degradation in color, low dose irradiation (up to 0.5 kGy) significantly (p≤0.05) increased the antioxidant activity of the plum peel anthocyanins.

scholarly journals Anthocyanin Induction by Drought Stress in the Calyx of Roselle Cultivars

Molecules ◽  
2020 ◽  
Vol 25 (7) ◽  
pp. 1555 ◽  
Author(s):  
Jeny Hinojosa-Gómez ◽  
César San Martín-Hernández ◽  
José B. Heredia ◽  
Josefina León-Félix ◽  
Tomás Osuna-Enciso ◽  
...  
Keyword(s):  
Water Stress ◽  
Abiotic Factors ◽  
Control Level ◽  
Ultra Performance Liquid Chromatography ◽  
Anthocyanin Content ◽  
Hibiscus Sabdariffa ◽  
Chemical Profile ◽  
Moisture Condition ◽  
Total Anthocyanin ◽  
Total Anthocyanin Content

Abiotic factors can alter the chemical profile of crops and the number of compounds they contain. In this study, the anthocyanin and anthocyanidin contents, determined by ultra-performance liquid chromatography (UPLC-MS/MS), and the colour attributes of the calyces of three cultivars of Hibiscus sabdariffa subjected to three water stress regimes during the stage of physiological maturity were investigated. The total anthocyanin content in calyx increased relative to the control content under a 65% moisture irrigation regime. Among the cultivars, UAN16-2 showed the greatest increases in the contents of cyanidin, delphinidin 3-O-glucoside, cyanidin 3-O-glucoside, and cyanidin 3-O-sambubioside. The content of cyanidin 3-O-sambubioside showed the greatest increase, increasing by 55% relative to the control level. The contents of these compounds are correlated with colour attributes such as luminosity. Water stress under the 33% moisture condition during plant development led to decreased anthocyanin contents in all of the roselle cultivars.

Production of Sterigmatocystin

1975 ◽  
Vol 58 (3) ◽  
pp. 622-623
Author(s):  
Martin Steyn ◽  
Chris J Rabie
Keyword(s):  
Crude Extract ◽  
Aspergillus Versicolor ◽  
Chromatographic Purification ◽  
Column Chromatographic

Abstract A procedure for the isolation of pure sterigmatocystin is described. It involves cultivation of an appropriate fungus on sterile maize, and extraction and column chromatographic purification of the crude extract. With this method sterigmatocystin may be obtained at a fraction of its commercial cost. Aspergillus versicolor seemed to be the best producer (5–12 g/kg) in 250 ml Erlenmeyer flasks. The yield dropped markedly with increasing flask size.

Extraction and Purification of Tea Polyphenols and EGCG

2013 ◽  
Vol 662 ◽  
pp. 392-395 ◽  
Author(s):  
Yan Hua Gao ◽  
Ying Chen ◽  
Zhen Xi Wang
Keyword(s):  
Green Tea ◽  
Silica Gel ◽  
Hollow Fiber ◽  
Membrane Filtration ◽  
Hollow Fiber Membrane ◽  
Tea Polyphenols ◽  
Fiber Membrane ◽  
Extraction And Purification ◽  
Column Chromatographic

The extraction and purification of tea polyphenols(TP) and EGCG from green tea extraction by nano-membrane filtration, resins absorption and column chromatographic exctration(CCE) were studied. The results indicated that the concentrations of TP and EGCG respectively increased to 35.21% and 13.56% after separated by the hollow fiber membrane with the molecular of 1.0×104. And then, after the absorption of polyamide resins, the concentrations further increased to 95.51% and 52.56%. Finally, the product with the EGCG concentration of 98% was obtained by silica gel CCE method.

Dry-column chromatographic purification of aflatoxin precursors

1980 ◽  
Vol 2 (1) ◽  
pp. 17-22 ◽  
Author(s):  
J. J. Dunn ◽  
L. S. Lee ◽  
J. W. Bennett
Keyword(s):  
Chromatographic Purification ◽  
Column Chromatographic

scholarly journals The right of information privacy between the challenges of technology and the reality of legal protection: الحق في الخصوصية المعلوماتية بين تحديات التقنية وواقع الحماية القانونية

2016 ◽  
Vol 2 (5) ◽  
Author(s):  
Ben Qara Mustafa Aisha
Keyword(s):  
Analytical Method ◽  
Personal Data ◽  
Legal Protection ◽  
Digital Age ◽  
Information Privacy ◽  
Legal Rules ◽  
Technical Challenges ◽  
The Individual ◽  
The Right ◽  
The Impact

This study aimed to identify the international, regional and even national efforts to protect the rights and privacy of the individual from the impact of informatics, and the extent to which it succeeded in achieving this. To achieve this, the researcher used the analytical method by explaining the new technical challenges to personal data and various legal mechanisms to protect this right. The research was based on an introduction, two papers and a conclusion. The first topic was entitled "What is the privacy of informatics and the dangers it faces in the digital age", while the second topic is devoted to international and regional efforts to protect information privacy. The results of the study showed that most of the legislations, especially the Arab ones, are not able to deal with violations of personal data, and concluded that new legal rules must be enacted to protect information privacy, based on established international principles in the field of informatics.

Sign in / Sign up

Export Citation Format

Share Document