scholarly journals Phenolic Isomers from Plantago Catharinea Leaves: Isolation, Identification, Quantification and in Vitro Antioxidant Activity

2017 ◽  
Vol 12 (3) ◽  
pp. 1934578X1701200
Author(s):  
Leonardo Mendes De Souza Mesquita ◽  
Claudia Quintino Da Rocha ◽  
Luiz Henrique Lima Affonso ◽  
Antonietta Cerulli ◽  
Sonia Piacente ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Chemical Structure ◽  
Biological Activities ◽  
Bioactive Molecules ◽  
Good Source ◽  
Rare Sugar ◽  
Polyphenolic Acids ◽  
In Vitro Antioxidant Activity

In this study we isolated two polyphenolic acids of m/z 639, called catharinol A and catharinol B, from Plantago catharinea L. (Plantaginaceae) leaves. Although presenting very similar structures, catharinol A showed higher antioxidant activity when compared with gallic acid and quercetin standards. These compounds are position isomers and present in their chemical structure the rare sugar D-allose. Molecules with similar constitution are known to have important biological activities such as antitumor and immunosuppressive. These compounds were isolated by high-performance liquid chromatography (HPLC) and characterized by mass spectrometry (FIA-ESI-IT-MS/MS) and nuclear magnetic resonance (NMR). This work is the first study on the chemical composition of P. catharinea and encourages the production of Plantago species as a good source of bioactive molecules.

scholarly journals To perform phytochemical screening and study the antioxidant potential of isolated compound from Hemidesmus indicus

2019 ◽  
Vol 9 (2) ◽  
pp. 188-191
Author(s):  
, Nutan ◽  
Manas Kr Das ◽  
Gaurav Saxena ◽  
Nitin Kumar
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Biological Activities ◽  
Phytochemical Screening ◽  
Good Source ◽  
Physicochemical Composition ◽  
Hemidesmus Indicus ◽  
Isolated Compound ◽  
In Vitro Antioxidant Activity

“Anantmul” is an important and widely used medicinal plant. The study aimed to determine the physicochemical composition, bioactive compounds and antioxidant activity of Hemidesmus indicus (Asclepiadaceae). The roots of the plant were collected and sequentially extracted using petroleum ether, ethyl acetate, and methanol. The preliminary phytochemical screening of extracts was carried out and found to be a good source of bioactive compounds. Biological activities of flavonoid and phenolic compounds have been discovered in several latest studies. Further phytochemical isolation was carried out, and Lupeol was isolated. Phenols are shown to be multifunctional antioxidants which will perform as singlet oxygen quenchers. Lupeol was evaluated for in-vitro antioxidant activity. It showed a correlation with antioxidant activity by DPPH (IC50 = 0.52, P 6 0.05) and H2O2 (IC50 = 0.43, P 6 0.05). The results show promising perspectives for the exploitation and use of anantmul rhizome as a constituent of anti-aging as well as anticancer diet.  Keywords: Anantmul, Lupeol, Antioxidant, DPPH 

scholarly journals Chromatographic and Spectroscopic Fingerprinting of Ficus carica and Evaluation of In Vitro Antioxidant Activity

2021 ◽  
Vol 25 (03) ◽  
pp. 677-682
Author(s):  
Amara Javaid
Keyword(s):  
Antioxidant Activity ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Potential ◽  
Ficus Carica ◽  
In Vitro Antioxidant Activity

The present study was conducted to evaluate the in vitro antioxidant activity of Ficus carica, commonly known as fig. Methanol and ethanol extracts of F. carica leaves were subjected to 2, 2-diphenyl-1-picryhydrazyl (DPPH) free radical scavenging activity assay where ascorbic acid being positive control had an IC50 value of 3.98±0.26 while methanol and ethanol fractions showed an IC50 of 101.76±1.12 and 93.12±1.17 respectively exhibiting their high antioxidant potential. DPPH assay was also performed on high performance liquid chromatography (HPLC) elutions. Most active antioxidant components in ethanol extract were eluted between 17–18 min, and those in methanol were eluted over 14–15 min and upon ultra-high performance liquid chromatography-mass spectrometery (Orbitrap Liquid Chromatography-Mass Spectrometry) were identified to be 13-Docosenamide, (Z)- for ethanol and ficusin for methanol fraction. Thus, it is concluded that these two components are most probable determinants of antioxidant potential of F. carica leaf extracts. © 2021 Friends Science Publishers

scholarly journals Polyphenolic Composition and In Vitro Antioxidant Activity of Red Grape Seeds as Byproducts of Short and Medium-Long Fermentative Macerations

Foods ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1451 ◽  
Author(s):  
Antonella Bosso ◽  
Claudio Cassino ◽  
Silvia Motta ◽  
Loretta Panero ◽  
Christos Tsolakis ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Grape Seeds ◽  
Antioxidant Power ◽  
Polyphenolic Composition ◽  
Polyphenolic Content ◽  
Red Grape ◽  
Polyphenolic Profile ◽  
In Vitro Antioxidant Activity

The polyphenolic composition and antioxidant activity of grape seeds, as byproducts of red winemaking, depend on various factors, such as grape cultivar, vintage effect, grape maturity and winemaking methods. In the present work, the influence of the maceration length on the polyphenolic and antioxidant characteristics of the seeds of four Italian red grape cultivars (‘Barbera’, ‘Grignolino’, ‘Nebbiolo’, and ‘Uvalino’), sampled from the fermentation tanks after short (two days) and medium-long (7–21 days) macerations, was studied with spectrophotometric methods, high-performance liquid chromatography (HPLC), and three different antioxidant assays (2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), ferric reducing antioxidant power (FRAP) and 2,2 diphenyl-1-picrylhydrazyl (DPPH)). The total polyphenolic content (gallic acid equivalent (GAE)) of the seeds sampled after short macerations ranged between 24.5 and 60.1 mg/g dry weight (DW), and it dropped to 20.0–37.5 mg/g DW after medium-long macerations. The polyphenolic profile of the shortly macerated seeds was related to the varietal characteristics, while, after longer macerations, the influence of the maceration length prevailed on the cultivar effect. The multiple in vitro antioxidant activity tests (ABTS, FRAP and DPPH), although based on different mechanisms capable of highlighting behavioral differences between the different polyphenolic compounds, were highly correlated with each other and with the polyphenolic parameters; the qualitative differences between the matrices in the polyphenolic profile were probably less important than the quantitative differences in the polyphenolic content. The relations with the polyphenolic content were linear, except for the Efficient Concentration (EC20) parameter, whose relation was better described by a hyperbolic equation.

scholarly journals Characterization and Biological Activities of an Alkali Soluble Exopolysaccharide from Acetobacter

2019 ◽  
pp. 1-10 ◽  
Author(s):  
Hema Chandran ◽  
Kanika Sharma
Keyword(s):  
Antioxidant Activity ◽  
Biological Activities ◽  
Stationary Phases ◽  
High Moisture ◽  
Moisture Retention ◽  
Viscous Solutions ◽  
Potential Use ◽  
Biomedical Fields ◽  
In Vitro Antioxidant Activity

Natural polysaccharides have received much attention due to the plethora of their applications. Acetobacter sp. MRL 5A (KX082688) produced an alkali soluble exopolysaccharide (20.39 g/L) on sucrose based media during the late log and early stationary phases. The exopolysaccharide was insoluble in water with high moisture-retention ability and produced viscous solutions and gels. The exopolysaccharide contained 40.3% glucose, 34.4% galactose, 22.2% mannose and 3.1% of arabinose monomers revealed by GC. The exopolysaccharide showed emulsifying, flocculating and in-vitro antioxidant activity similar to standard exopolysaccharides like dextran and xanthan. This EPS can be of potential use in food, cosmetic, pharmaceutical and biomedical fields.

Antioxidant Components and Antioxidant Activities of Hydroglyceroalcoholic Extract from Fresh Mushroom Mycelium

2017 ◽  
Vol 68 (3) ◽  
pp. 534-540
Author(s):  
Emanuel Vamanu
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Phenolic Acid ◽  
Antioxidant Activities ◽  
Submerged Cultivation ◽  
Active Components ◽  
Gentisic Acid ◽  
Polyphenolic Acids ◽  
In Vitro Antioxidant Activity

The in vitro antioxidant activity, as well as the hydroglyceroalcoholic extracts main active components from mycelia of Pleurotus ostreatus and Coprinus comatus, were determined. The mycelium submitted for extraction was obtained by submerged cultivation in a bioreactor. The hydroglyceroalcoholic extracts, in particular, those of P. ostreatus PSI101109 and PQMZ91109 mycelium had a considerable antioxidant activity, and was compared to the overall constituent of flavonoids plus phenolic compounds. According to the extracts HPLC assessment, the key phenolic acid evident from the extracts was gentisic acid. The homogentisic, gallic and protocatechuic acids were also determined. Catechin was identified as well, except for C. comatus M8102 and P. ostreatus PBS281009 mycelia extracts. Since catechol and gentisic acid levels were high, respectively, there was a correlation with the absence of carotenoid compounds in hydroglyceroalcoholic extracts (P. ostreatus PSI101109). There is a correspondence between the hydroglyceroalcoholic extracts� key antioxidant activities with the catechin and polyphenolic acids levels identified in each extract.

scholarly journals Cannabidiol Content and In Vitro Biological Activities of Commercial Cannabidiol Oils and Hemp Seed Oils

Medicines ◽  
2020 ◽  
Vol 7 (9) ◽  
pp. 57
Author(s):  
Masashi Kitamura ◽  
Yuka Kiba ◽  
Ryuichiro Suzuki ◽  
Natsumi Tomida ◽  
Akemi Uwaya ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Seed Oil ◽  
Cannabis Sativa ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Seed Oils ◽  
Hemp Seed ◽  
Industrial Hemp

Background: Hemp (Cannabis sativa L.) seed contains high contents of various nutrients, including fatty acids and proteins. Cannabidiol (CBD) is a non-psychoactive compound that can be extracted from C. sativa and used for treating epilepsy and pain. Industrial hemp products, including CBD and hemp seed oils, have become increasingly popular. Some products are marketed without a clear distinction between CBD and hemp seed oils. Herein, the CBD content and biological activities of commercial CBD and hemp seed oils were examined. Methods: CBD content was measured by high-performance liquid chromatography. For in vitro antioxidant activity determination, 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) radical-scavenging assays were performed. Results: The CBD concentrations in the two CBD oil samples were 18.9 ± 0.5 and 9.2 ± 0.4 mg/mL. Of the seven hemp seed oil samples, six samples contained CBD in concentrations ranging from 2.0 ± 0.1 to 20.5 ± 0.5 µg/mL, but it was not detected in one sample. Antioxidant activity was observed in both CBD oil samples. Conclusions: The results indicate that (1) CBD content varied by hemp seed oil sample and that (2) antioxidant activity could be a useful landmark for discriminating CBD oils from hemp seed oils.

scholarly journals Free and Conjugated Phenolic Profiles and Antioxidant Activity in Quinoa Seeds and Their Relationship with Genotype and Environment

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1046
Author(s):  
Fabiana Antognoni ◽  
Giulia Potente ◽  
Stefania Biondi ◽  
Roberto Mandrioli ◽  
Lorenzo Marincich ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Environmental Conditions ◽  
High Performance ◽  
Biological Activities ◽  
Chenopodium Quinoa ◽  
Northern Italy ◽  
Temperate Climate ◽  
Total Phenolic ◽  
Array Detector

The nutraceutical interest in quinoa (Chenopodium quinoa Willd.) seeds is associated with the presence of macronutrients, micronutrients, minerals, vitamins, and polyphenols. In particular, polyphenols contribute to the health-promoting effects of this food crop, and their levels are influenced by environmental conditions. Production of quinoa is recently being explored in temperate climate areas, including Italy. The aim of this research was to assess the profile of bioactive compounds in seeds of two quinoa varieties, Regalona-Baer and Titicaca, grown in northern Italy, compared to that of seeds of those varieties grown in Chile and Denmark, respectively. High-performance liquid chromatography-diode array detector (HPLC-DAD) analysis of phenolic acid and flavonoid profiles, both in their free and soluble conjugated forms, showed that the main differences between Regalona grown in Chile and Italy were for the free vanillic acid and daidzein contents, while the two Titicaca samples mainly differed in quercetin derivative levels. The total phenolic index was comparable in Titicaca and Regalona, and only a slight decrease in this parameter was found in seeds of the two varieties grown in Italy. The in vitro antioxidant activity of seed extracts, evaluated by means of three different assays, indicated that it correlated with flavonol (quercetin derivative) levels. In conclusion, the results indicate that, although environmental conditions alter the polyphenolic profile and biological activities, it is possible to grow good-quality quinoa in northern Italy.

scholarly journals Chemical composition and biological activities of essential oils of endemic Thymus leucostomus Hausskn. et Velen

2020 ◽  
Vol 49 (4) ◽  
pp. 957-965
Author(s):  
Omer Elkiran ◽  
Cumhur Avsar
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Biological Activities ◽  
Antioxidant Properties ◽  
Antimicrobial Activities ◽  
Gram Positive Bacteria ◽  
In Vitro Antioxidant Activity

The chemical composition, antimicrobial and antioxidant properties of the essential oils from the leaves of endemic Thymus leucostomus naturally grown in Turkey were investigated and chemical differences were discussed by means of chemotaxonomy. Twenty-six components were identified representing 98.8% of the oils. The main compounds in the essential oil of T. leucostomus were: o-cymene (30.6%), carvacrol (9.6%), thymol methyl ether (7.2%), limonene (6.8%). Essential oil was screened for their antimicrobial activities against 7 bacteria and 2 yeast species by using disc-diffusion and MIC procedure. The essential oil showed higher effectiveness against all the tested bacteria and yeast. The extract was observed to be much more effective in Gram-positive bacteria (especially, S. aureus ATCC 6538). In vitro antioxidant activity based on the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical was evaluated for the essential oil, and it was found that the essential oil had good antioxidant activity in the range of the IC50= 5.42 ±0.8 μg/ml.

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