Two Diarylundecanones Isolated from Ardisia tenera

2017 ◽  
Vol 12 (9) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Chong-Yu Lu ◽  
Hong-Xiang Li ◽  
Sha Li ◽  
Ling Qiu ◽  
Si Yu ◽  
...  
Keyword(s):  
Cell Lines ◽  
Ethanol Extract ◽  
Spectroscopic Evidence ◽  
Aerial Parts ◽  
Inhibitory Activities ◽  
Mcf 7

Ardisinones G-H (1–2), two new diarylundecanones, were isolated from the ethanol extract of aerial parts of Ardisia tenera Mez. Their structures were elucidated by interpretation of the spectroscopic evidence. The isolated compounds were evaluated for cytotoxicity against four cell lines (MV4–11, MCF-7, HCT116 and A549) and displayed moderate inhibitory activities.

scholarly journals In Vitro Cytotoxic Effects of Secondary Metabolites Present in Sarcopoterium Spinosum L.

2021 ◽  
Vol 11 (11) ◽  
pp. 5300
Author(s):  
Jozef Hudec ◽  
Jan Mojzis ◽  
Marta Habanova ◽  
Jorge A. Saraiva ◽  
Pavel Hradil ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Benzalkonium Chloride ◽  
Ethanol Extract ◽  
Cytotoxic Activities ◽  
Sarcopoterium Spinosum ◽  
Mcf 7

Sarcopoterium spinosum (L.) is a medicinal plant traditionally used for the treatment of various diseases including cancer in the Near- and Middle East. The fractions and constituents of the ethanol extract of S. spinosum were screened for in vitro cytotoxic activities on Jurkat (acute T-lymphoblastic leukemia), HeLa (cervical adenocarcinoma), MCF-7 (mammary gland adenocarcinoma), Caco-2 (human colorectal adenocarcinoma), and MDA-MB-231 (mammary gland adenocarcinoma) cell lines using the MTT (3-(dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The ethanol extract was subsequently re-extracted with ethyl acetate and in its sub-fraction obtained by column chromatography three compounds (stachydrine, benzalkonium chloride and rutine) were the first time identified by nuclear magnetic resonance (NMR) analyses. The most active subfraction showed cytotoxic activity against HeLa, MCF-7, and Caco-2 cell lines. The three compounds mentioned, as standards of high-performance liquid chromatography (HPLC) quality, were studied individually and in combination. Cytotoxic activity observed might be due to the presence of benzalkonium chloride and rutin. Benzalkonium chloride showed the strongest growth suppression effect against HeLa cells (IC50 8.10−7 M) and MCF-7 cells (IC50 5.10−6 M). The mixture of stachydrine and benzalkonium chloride allowed a synergistic cytotoxic effect against all tested cancer and normal cells to be obtained. Anti-cancer activity of the plant extract of S. spinosum remains under-investigated, so this research describes how the three major compounds identified in the ethyl acetate extract can exert a significant dose dependent in vitro cytotoxicity.

scholarly journals Bauhinia variegataLeaf Extracts Exhibit Considerable Antibacterial, Antioxidant, and Anticancer Activities

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Amita Mishra ◽  
Amit Kumar Sharma ◽  
Shashank Kumar ◽  
Ajit K. Saxena ◽  
Abhay K. Pandey
Keyword(s):  
Cell Lines ◽  
Metal Ion ◽  
Human Cancer ◽  
Reducing Power ◽  
Ethanol Extract ◽  
Anticancer Activities ◽  
Leaf Extracts ◽  
Human Cancer Cell Lines ◽  
Polar Extracts ◽  
Mcf 7

The present study reports the phytochemical profiling, antimicrobial, antioxidant, and anticancer activities ofBauhinia variegataleaf extracts. The reducing sugar, anthraquinone, and saponins were observed in polar extracts, while terpenoids and alkaloids were present in nonpolar and ethanol extracts. Total flavonoid contents in various extracts were found in the range of 11–222.67 mg QE/g. In disc diffusion assays, petroleum ether and chloroform fractions exhibited considerable inhibition againstKlebsiella pneumoniae. Several other extracts also showed antibacterial activity against pathogenic strains ofE. coli,Proteusspp. andPseudomonasspp. Minimum bactericidal concentration (MBC) values of potential extracts were found between 3.5 and 28.40 mg/mL. The lowest MBC (3.5 mg/mL) was recorded for ethanol extract againstPseudomonasspp. The antioxidant activity of the extracts was compared with standard antioxidants. Dose dependent response was observed in reducing power of extracts. Polar extracts demonstrated appreciable metal ion chelating activity at lower concentrations (10–40 μg/mL). Many extracts showed significant antioxidant response in beta carotene bleaching assay. AQ fraction ofB. variegatashowed pronounced cytotoxic effect against DU-145, HOP-62, IGR-OV-1, MCF-7, and THP-1 human cancer cell lines with 90–99% cell growth inhibitory activity. Ethyl acetate fraction also produced considerable cytotoxicity against MCF-7 and THP-1 cell lines. The study demonstrates notable antibacterial, antioxidant, and anticancer activities inB. variegataleaf extracts.

scholarly journals Cytotoxic potential of ethanol extract of Parquetina nigrescens on MCF-7, C4-2WT, HT 29 and HTC 116 cell lines

2018 ◽  
Vol 12 (23) ◽  
pp. 310-318
Author(s):  
M. Onyegeme-Okerenta Blessing ◽  
Agyare Christian ◽  
D. Bradshaw Tracey ◽  
A. Spriggs Keith
Keyword(s):  
Cell Lines ◽  
Ethanol Extract ◽  
Cytotoxic Potential ◽  
Ht 29 ◽  
Mcf 7

scholarly journals Chemical Composition and Biological Activity of Pulicaria vulgaris Essential Oil from Iran

2014 ◽  
Vol 9 (11) ◽  
pp. 1934578X1400901 ◽  
Author(s):  
Javad Sharifi-Rad ◽  
Abdolhossein Miri ◽  
Seyedeh Mahsan Hoseini-Alfatemi ◽  
Majid Sharifi-Rad ◽  
William N. Setzer ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Cell Lines ◽  
Chemotherapeutic Agents ◽  
Cytotoxic Activities ◽  
Hep G2 ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Aerial Parts ◽  
Mcf 7

The present study investigated the chemical composition of the essential oil (EO) from aerial parts (flowering stage) of Pulicaria vulgaris Gaertn. by GC–MS. Also, the antimicrobial activity of the EO against Gram-positive bacteria ( Bacillus cereus and Staphylococcus aureus), Gram-negative bacteria ( Escherichia coli and Pseudomonas aeruginosa) and fungi ( Aspergillus niger and Candida albicans) was tested. In total, 23 compounds were recognized, accounting for 98.08 % of the EO. The main compounds in the EO were thymol (50.22%), p-menth-6-en-2-one (carvotanacetone, 20.2%), thymol isobutyrate (16.88%), menthan-2-one (4.31%), 1-methyl-1,2-propanedione (4.13%), 2,5-dimethoxy- p-cymene (4.01%), myrtenol (1.22%), linalool (1.1%), and β-myrcene (1.9%). Results of antibacterial test of P. vulgaris essential oil showed that all assayed concentrations significantly inhibited the growth of B. cereus, S. aureus, E. coli, and P. aeruginosa at P < 0.05. MIC for B. cereus, S. aureus, E. coli, P. aeruginosa was 17.5, 25.2, 19.4 and 33.2 μg/mL respectively; antifungal screening of the essential oil of P. vulgaris showed that the oil significantly inhibited the growth of A. niger and C. albicans (MIC = 15.5 and 9.9 μg/mL, respectively). Results of cytotoxicity assay showed that the essential oil exhibited a significant cytotoxic activity against both cell lines. In case of MCF-7 and Hep-G2 cell lines, IC50 of the essential oil were 5.36 and 7.16 μμg/ml, respectively. The potent antimicrobial and cytotoxic activities of the EO may be attributed to its high contents of thymol, carvotanacetone and thymol isobutyrate. Antimicrobial and antitumor chemotherapies are showing diminishing effectiveness because of emergence of drug-resistance. Hence, using efficient natural chemotherapeutic agents such as Pulicaria vulgaris essential oil with fewer side effects is an encouraging approach to fight cancer and infectious diseases in medicine, agriculture, food science and related fields.

scholarly journals New Cytotoxic Cycloartane Triterpenes from the Aerial Parts of Actaea heracleifolia (syn. Cimicifuga heracleifolia)

Planta Medica ◽  
2018 ◽  
Vol 85 (02) ◽  
pp. 154-159
Author(s):  
Qiang-Qiang Shi ◽  
Wei-Hua Wang ◽  
Jing Lu ◽  
Da-Shan Li ◽  
Lin Zhou ◽  
...  
Keyword(s):  
Cell Lines ◽  
Human Tumor ◽  
Human Tumor Cell ◽  
Human Tumor Cell Lines ◽  
Chemical Structures ◽  
Aerial Parts ◽  
Ic50 Values ◽  
Weak Activity ◽  
Mcf 7

AbstractOne new 15,16-seco-cycloartane triterpene (1), three new cycloartane triterpene glycosides (2–4), and five known compounds (5–9) were isolated from the aerial parts of Actaea heracleifolia. The chemical structures of these compounds were determined on the basis of NMR analysis, HRTOF-ESIMS data, and other spectroscopic methods. Selected compounds were evaluated for their cytotoxicity against human tumor cell lines (HL-60, SMMC-7721, A549, MCF-7, and SW480) in vitro. Compounds 3 and 4 showed weak activity against the HL-60, A-549, and MCF-7 cell lines with IC50 values ranging from 21.34 to 36.98 µM.

scholarly journals New Cytotoxic Guaianolides from Centaurea Aegyptiaca

2016 ◽  
Vol 11 (6) ◽  
pp. 1934578X1601100
Author(s):  
Hanan G. Sary ◽  
Abdel Nasser B. Singab ◽  
Khaled Y. Orabi
Keyword(s):  
Cytotoxic Activity ◽  
Spectral Data ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Ethanol Extract ◽  
Aerial Parts ◽  
Carcinoma Cell Lines

The ethanol extract of Centaurea aegyptiaca aerial parts afforded two new chlorinated bioactive guaianolides, cenegyptin A (1) and cenegyptin B (2), in addition to four known sesquiterpenes (3-6). Their identities were established on the basis of their spectral data. The cytotoxicity (IC50, μM) of compounds 1-6 were evaluated against hepatic (HEPG2) and laryngeal (HEP2) carcinoma cell lines in comparison with normal fibroblasts (BHK). Compound 1 showed cytotoxic activity against HEPG2 and HEP2 with IC50 values of 7.2 ± 0.04 and 7.5 ± 0.02, respectively. However, compound 2 exhibited only a limited toxicity against both cell lines.

scholarly journals Ethanol extract of male Carica papaya flowers demonstrated non-toxic against MCF-7, HEP-G2, HELA, NCI-H460 cancer cell lines

2020 ◽  
Vol 58 (1) ◽  
pp. 86-91 ◽  
Author(s):  
Vo Thi Nga ◽  
Nguyen Thi Hanh Trang ◽  
Nguyen Thi Anh Tuyet ◽  
Nguyen Kim Phi Phung ◽  
Ngo Thi Thuy Duong ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Carica Papaya ◽  
Ethanol Extract ◽  
Cancer Cell Lines ◽  
Hep G2 ◽  
Mcf 7

scholarly journals Potential Antiproliferative Activity and Evaluation of Essential Oil Composition of the Aerial Parts of Tamarix aphylla (L.) H.Karst.: A Wild Grown Medicinal Plant in Jordan

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
N. Alhourani ◽  
V. Kasabri ◽  
Y. Bustanji ◽  
R. Abbassi ◽  
M. Hudaib
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Human Fibroblasts ◽  
Cancer Cell Lines ◽  
Cytotoxic Agents ◽  
Normal Fibroblast ◽  
Breast Adenocarcinoma ◽  
Dependent Manner ◽  
Aerial Parts ◽  
Mcf 7

Essential (volatile) oil from aerial parts of Tamarix aphylla (L.) H.Karst. (Tamaricaceae) grown wild in Jordan was hydrodistilled by Clevenger apparatus and analyzed by means of GC and GC-MS techniques. In vitro screening of potential cytotoxicity of the aqueous (AE) and ethanol (EE) extracts was also evaluated against human breast adenocarcinoma (MCF-7), colorectal adenocarcinoma (Caco-2), and pancreatic carcinoma (Panc-1) cancer cell lines as well as normal human fibroblasts. GC-MS analysis of T. aphylla EO revealed its richness in nonterpenoid nonaromatic hydrocarbons (52.39%), with predominance of 6,10,14-trimethyl-2-pentadecanone as the principal component. Biologically, the plant extracts exhibited cytotoxicity effects in dose-dependent manner against most of the tested cell lines, but potent effects were only predicted against MCF-7 cells with IC50 values of 2.17 ± 0.10 and 26.65 ± 3.09 μg/mL for T. aphylla AE and EE, respectively. T. aphylla AE demonstrated a comparable cytotoxic effect with that offered by the control drug cisplatin (IC50 value of 1.17 ± 0.13 μg/mL), even with higher safety profile against normal fibroblast cells (IC50 values of T. aphylla AE versus cisplatin: 79.99 ± 4.90 versus 9.08 ± 0.29 μg/mL). T. aphylla extracts could be a valuable source for cytotoxic agents with high safety and selective cytotoxicity profiles. Unfortunately, no antiproliferative potential against Caco-2 or Panc-1 cancer cell lines was detected at a concentration less than 30 μg/mL.

The Cytotoxic Effect of Ethanol Extract of Momordica Charantia, Kuguacin-J and Cisplatin on Healthy MCF-10A and MCF-7 and MDAMB-231 Breast Cancer Cell Lines in Vitro

2021 ◽  
Author(s):  
Chahinez Houacine ◽  
Jaipaul Singh ◽  
Raphael Singh ◽  
Karishma Jeeboo ◽  
Abdullah Adil Ansari ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Death ◽  
Cell Viability ◽  
Cell Lines ◽  
Caspase 3 ◽  
Ethanol Extract ◽  
Momordica Charantia ◽  
High Dose ◽  
Time Points ◽  
Mcf 7

Abstract Traditional medicines, derived from plants, could present alternative treatment strategy for cancer therapy. One such plant is Momordica charantia (MC) which possesses anti-carcinogenic properties. This study investigated the anticancer effect of an ethanol extract of MC fruit, Kuguacin-J (K-J), an isolated compound from the leaves of MC and cisplatin, either alone or combination on healthy MCF-10A mammary cells and compared with breast cancer MCF-7 and MDAMB-231 cell lines. Cell viability was tested using 8 μg/mL and 80 μg/mL doses of MC extract, K-J and cisplatin individually or combined for 24 and 48 hours. Caspase-3- activity was measured in MCF-7 and MDA-MB-231 cells using established methods. The results revealed that MC extract and K-J had no effect on healthy MCF-10A cell viability as compared to cisplatin which induced dose and time-dependent cell death. Similarly, treatment of MCF-7 cells with cisplatin induced cell death at high concentration at both the time points, while MC extract and K-J only induce MCF-7 cell death at high dose after 48 hours only. During combination therapy, both doses of cisplatin enhanced MCF-7 cell death when combined with MC extract or K-J after 24 and 48 hours. In MDAMB-231 cells, the three drugs, either alone or combined, evoked significant cell death at both the doses and time points. All three drugs, at high dose, elicited significant increase in caspase-3- activity in MCF-7 and MDA-MB-231 cells as compared to untreated cells. The results revealed that either MC extract or K-J alone or combined with cisplatin, can elicit significant increase in cell death and caspase–3-activity in MCF-7 and MDA-MB-231cells as compared to untreated cells.

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