Potential role of soluble TNF-α receptors in diagnosis of idiopathic membranous nephropathy

Soluble tumor necrosis factor alpha (TNF-α) receptors contain two receptors: soluble tumor necrosis factor receptor (sTNFR) 1 and 2, and the aim of our study was to discover their concentration and diagnostic value for idiopathic membranous nephropathy (IMN). In total, 58 patients with IMN, 51 patients with chronic kidney disease (CKD), and 30 healthy volunteers were enrolled in this study. Levels of serum sTNFR1 and sTNFR2 were determined by enzyme-linked immunosorbent assay (ELISA). Serum cystatin C (CysC), urea, creatinine (CREA), uric acid (UA), total protein (TP), albumin (ALB), and 24-h urinary protein (proteinuria, PRO) were examined by automatic biochemical analyzer. Levels of sTNFR1 and sTNFR2 were significantly higher in IMN group than CKD and control group ( P < 0.05). In IMN group, there were significant correlation between sTNFR1 and sTNFR2 ( P < 0.01). Both sTNFR1 and sTNFR2 were positively related to serum urea, CREA, CysC, UA, 24-h PRO ( P < 0.05) and negatively related to ALB ( P < 0.01). Receiver operating characteristic (ROC) analysis showed that the area under the curve (AUC) of sTNFR1 and sTNFR2 were 0.997 and 0.993, respectively, when control was healthy volunteers. When sTNFR1 cut-off was 959.15 pg/mL, the sensitivity and specificity were 96.6% and 100%, respectively. When sTNFR2 cut-off was 2449.43 pg/mL, the sensitivity and specificity were 93.1% and 100%, respectively. While the control was CKD group, AUC of sTNFR1 and sTNFR2 were 0.647 and 0.626, respectively. When sTNFR1 cut-off was 3356.57 pg/mL, the sensitivity and specificity were 72.4% and 60.8%, respectively. When sTNFR2 cut-off was 6497.34 pg/mL, the sensitivity and specificity were 72.4% and 58.8%, respectively. This is the first study to show that both levels of sTNFR1 and sTNFR2 increased and correlated with serum urea, CREA, CysC, UA, ALB, 24-h PRO and could be usable for IMN diagnosis and differential diagnosis between IMN and CKD.

Download Full-text

Modulation of Anti-Tumor Necrosis Factor Alpha (TNF-α) Antibody Secretion in Mice Immunized with TNF-α Kinoid

Clinical and Vaccine Immunology ◽

10.1128/cvi.05649-11 ◽

2012 ◽

Vol 19 (5) ◽

pp. 699-703 ◽

Cited By ~ 10

Author(s):

Eric Assier ◽

Luca Semerano ◽

Emilie Duvallet ◽

Laure Delavallée ◽

Emilie Bernier ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Tumor Necrosis Factor ◽

Tumor Necrosis Factor Alpha ◽

Tumor Necrosis ◽

Enzyme Linked Immunosorbent Assay ◽

Necrosis Factor Alpha ◽

Neutralizing Capacity ◽

Tnf Α ◽

Factor Alpha ◽

Necrosis Factor

ABSTRACTTumor necrosis factor alpha (TNF-α) blockade is an effective treatment for patients with TNF-α-dependent chronic inflammatory diseases, such as rheumatoid arthritis, Crohn's disease, and psoriasis. TNF-α kinoid, a heterocomplex of human TNF-α and keyhole limpet hemocyanin (KLH) (TNF-K), is an active immunotherapy targeting TNF-α. Since the TNF-K approach is an active immunization, and patients receiving this therapy also receive immunosuppressant treatment, we evaluated the effect of some immunosuppressive drugs on the generation of anti-TNF-α antibodies produced during TNF-K treatment. BALB/c mice were injected intramuscularly with TNF-K in ISA 51 adjuvant. Mice were also injected intraperitoneally with one of the following: phosphate-buffered saline, cyclophosphamide, methylprednisolone, or methotrexate. Anti-TNF-α and anti-KLH antibody levels were assessed by enzyme-linked immunosorbent assay and the anti-TNF-α neutralizing capacity of sera by L929 bioassay. Our results showed that current treatments used in rheumatoid arthritis, such as methylprednisolone and methotrexate, do not significantly alter anti-TNF-α antibody production after TNF-K immunization. In contrast, the administration of cyclophosphamide (200 mg/kg) after immunization significantly reduced anti-TNF-α antibody titers and their neutralizing capacity.

Download Full-text

PROFIL TNF-α SESUDAH SENAM LANSIA PADA LANSIA DI PANTI WREDHA BETHANIA LEMBEAN KOTA MANADO

Jurnal e-Biomedik ◽

10.35790/ebm.3.1.2015.7660 ◽

2015 ◽

Vol 3 (1) ◽

Author(s):

Michael Tulong ◽

Siantan Supit ◽

Joice N. A. Engka

Keyword(s):

Informed Consent ◽

Tumor Necrosis Factor ◽

Tumor Necrosis ◽

Tumor Necrosis Factor Α ◽

Enzyme Linked Immunosorbent Assay ◽

Test Design ◽

Tnf Α ◽

Post Test ◽

Factor Α ◽

Necrosis Factor

Abstract: Tumor Necrosis Factor- α (TNF-α) is a systemic pro-inflammatory cytokine which is responsible to a trauma, injury, or inflammation in human body. Physical activity can physiologically affect the human body, inter alia the immune system. This was an experimental study with a post test design. This study aimed to determine the profile of TNF-α after doing gymnastics among the elderly at nursing home Bethania Lembean. Samples included 30 peoples who met the inclusion criteria, i.e. over 64 years old, followed the gymnastics for 5 weeks regularly, approbated as respondents, healthy, and signed the informed consent. The TNF-α concentration measurements were done by using enzyme-linked immunosorbent assay (ELISA) Quantikine ®Human TNF-α. The results showed that the average conventration of TNF-α after elderly gymnastics was 70.54 pg/ml, with the highest value of 88.90 pg/mL and the lowest value of 12.54 pg/mL. For all respondents, TNF-α was within normal limit and ranged between 10-100 pg / mL. Conclusion: In this study, TNF-α concentrations after elderly gymnastics for 5 weeks were within normal limitsKeywords: TNF-α, elderly gymnasticsAbstrak: Tumor Necrosis Factor-α (TNF-α) merupakan suatu sitokin sistemik pro-inflamasi, yang berespons terhadap suatu cedera trauma atau peradangan yang terjadi dalam tubuh manusia. Kegiatan fisik dapat berdampak cukup besar pada tubuh manusia, dimana dapat merubah sistem imun tubuh secara fisiologis. Penelitian ini bersifat eksperimental dengan rancangan post test design dan bertujuan untuk mengetahui profil TNF-α sesudah senam lansia di Panti Wredha Bethania Lembean. Sampel penelitian berjumlah 30 orang yang memenuhi kriteria inklusi yaitu berumur di atas 64 tahun, teratur mengikuti senam lansia selama 5 minggu, bersedia menjadi responden, sehat saat di periksa, dan menandatangani informed consent. Pengukuran konsentrasi TNF-α di lakukan dengan menggunakan enzyme-linked immunosorbent assay (ELISA) Quantikine ®Human TNF-α. Hasil penelitian memperlihatkan bahwa rerata kadar TNF-α sesudah senam lansia 70,54 pg/ml, dengan nilai tertinggi 88,90 pg/mL dan nilai terendah 12,54 pg/mL. Berdasarkan hasil yang di dapat, semua kadar TNF-α masih dalam batas normal dengan nilai 10-100 pg/mL. Simpulan: TNF-α sesudah senam lansia selama 5 minggu masih dalam batas normal.Kata kunci:TNF-α, senam lansia

Download Full-text

Korelasi Tingkat Depresi dengan Kadar Tumor Necrosis Factor-Alpha (TNF-α) pada Penderita Asma Bronkial Tidak Terkontrol

Jurnal Penyakit Dalam Indonesia ◽

10.7454/jpdi.v3i2.12 ◽

2017 ◽

Vol 3 (2) ◽

pp. 74

Author(s):

Muhammad Ali Apriansyah ◽

Rudi Putranto ◽

Eddy Mart Salim ◽

Hamzah Shatri

Keyword(s):

Tumor Necrosis Factor ◽

Bronchial Asthma ◽

Tumor Necrosis Factor Alpha ◽

Tumor Necrosis ◽

Enzyme Linked Immunosorbent Assay ◽

Necrosis Factor Alpha ◽

Tnf Α ◽

Factor Alpha ◽

Α Level ◽

Necrosis Factor

Pendahuluan. Prevalensi depresi hamper mencapai 50% pada pasien yang berobat di pelayanan tertier klinik asma. Tumor Necrosis Factor-Alpha (TNF-α) telah diketahui sebagai sitokin pro-inflamasi yang berperan penting dalam mekanisme patogenesis sejumlah penyakit inflamasi kronik, termasuk asma bronkial dan depresi. Belum ada data penelitian mengenai hal tersebut di Indonesia.Metode. Penelitian ini merupakan studi cross sectional yang dilakukan pada 40 pasien asma bronkial tidak terkontrol di alergi imunologi klinik unit rawat jalan Rumah Sakit Umum Pusat (RSUP) Moh Hoesin Palembang selama kurun waktu mulai bulan Juni 2014 sampai dengan Agustus 2014. Asma bronkial tidak terkontrol dinilai dengan menggunakan kuesioner Asthma Control Test (ACT), sedangkan gejala depresi dinilai dengan kuisioner Beck Depression Inventory (BDI). Konfirmasi diagnosis depresi dilakukan dengan kriteria dari Diagnostic and Statistical Manual for Psychiatry-IV Text Revision (DSMIV TR)/ International Code Diagnose 10 (ICD-10). Sementara itu, kadar TNF-α serum diukur dengan metode quantitative enzyme-linked immunosorbent assay (ELISA).Hasil. Nilai median tingkat depresi dan TNF-α serum pada penelitian ini adalah 16 (10 – 45) dan 4,09 (1,29 – 19,57) pg/mL.Tidak didapatkan korelasi yang bermakna secara statistik antara tingkat depresi dan kadar TNF-α (r = -0,265, p = 0,098).Simpulan. Tidak didapatkan korelasi yang bermakna antara tingkat depresi dengan kadar TNF-α pada penderita asma bronkial tidak terkontrol.Kata Kunci: asma bronkial tidak terkontrol, kadar TNF-α, Tingkat depresi The Correlation of Depression Level with Tumor Necrosis Factor-Alpha (TNF-α) Concentration in Uncontrolled Bronchial Asthma PatientsIntroduction. Depression occurs at high rates in people with chronic diseases, including bronchial asthma, with the prevalence of depression approaches 50% in tertiary care asthma clinic. Tumor necrosis factor alpha (TNF-α) is known to play a critical role in the pathogenic mechanism of a number of chronic inflammatory disease, including bronchial asthma and depression. There has not been any research data on the subject in Indonesia. The objective of this study was to investigate the correlation between depressive level and TNF-α level in uncontrolled bronchial asthma. Methods. This was a cross sectional study conducted in 40 patients with uncontrolled bronchial asthma at the allergy immunology clinic outpatient of Dr Moh Hoesin Hospital Palembang, during June 2014 until August 2014. Uncontrolled bronchial asthma was assessed using the Asthma Control Test (ACT) questionnaire, whereas depressive symptoms were assessed by Beck Depression Inventory (BDI) questionnaire, and diagnosis was confirmed by the criteria of the Diagnostic and Statistical Manual for Psychiatry-IV Text Revision (DSM-IV TR) / International Code Diagnose 10 (ICD-10). Serum levels of TNF-α was measured by the method of quantitative enzyme-linked immunosorbent assay (ELISA). Results. The median value of the level of depression and serum TNF- α in this study were 16 (10 - 45) and 4.09 (1.29 - 19.57) pg/mL. There was no significant correlation between depressive level and TNF-α level ( r = -0.265 , p = 0.098 ). Conclusions. There was no significant correlation between depressive level and TNF-α level in uncontrolled bronchial asthma Keywords: depressive level, TNF-α level, uncontrolled asthma bronchial

Download Full-text

Lipopolysaccharide influence on leptin hormone and tumor necrosis factor-alpha release from human adipose tissue

European Journal of Inflammation ◽

10.1177/2058739218774975 ◽

2018 ◽

Vol 16 ◽

pp. 205873921877497

Author(s):

Sa’ad Al-Lahham ◽

Nidal Jaradat ◽

Malik Al-Qub ◽

Abdallah Hamayel ◽

Abdalrahman Assaassa ◽

...

Keyword(s):

Adipose Tissue ◽

Tumor Necrosis Factor ◽

Tumor Necrosis ◽

Ex Vivo ◽

Enzyme Linked Immunosorbent Assay ◽

Low Grade ◽

Necrosis Factor Alpha ◽

Tnf Α ◽

Low Grade Inflammation ◽

Necrosis Factor

Obesity is associated with low-grade inflammation that originates mainly from adipose tissue. This is implicated in the pathogenesis of type-2 diabetes and cardiovascular diseases. Strong evidence indicates that chronically elevated systemic low-grade lipopolysaccharide (LPS), elicits low-grade inflammation. However, evidence on LPS effect on adipokines’ level, such as leptin, is scarce, and it has never been investigated ex vivo in human subcutaneous adipose tissue (SAT) and therefore we aim to investigate this. To achieve our aim, SAT explants were obtained from 12 patients (50% males) and were treated with/without LPS. Protein concentration was determined by enzyme-linked immunosorbent assay (ELISA). We found that the average age and body mass index (BMI) of included patients were 58.6 years and 28.6 kg/m2, respectively. LPS induced significantly (~3×, P < 0.0001) the secretion of tumor necrosis factor (TNF)-α from SAT, and it was not associated with age or BMI. However, leptin secretion was inhibited slightly (~20%), but significantly. Interestingly, leptin release was significantly inhibited (~50%) in SAT from lean but not from obese patients, and there was an association between leptin response and BMI (R = 0.8), but no association with age. In this study, we found, for the first time, that LPS suppresses the release of leptin hormone from SAT obtained from lean patients, while it induces TNF-α release. Our findings provide extra evidence and confirm earlier studies regarding the role of LPS in low-grade inflammation. Further investigations are essential to identify factors that inhibit LPS passage through intestinal barrier in order to prevent or reduce the development of obesity and its associated chronic diseases.

Download Full-text

Gender-Related Cytokine Patterns in Sera of Schistosomiasis Patients with Symmers' Fibrosis

Clinical and Diagnostic Laboratory Immunology ◽

10.1128/cdli.11.3.627-630.2004 ◽

2004 ◽

Vol 11 (3) ◽

pp. 627-630 ◽

Cited By ~ 10

Author(s):

David Nascimento Silva-Teixeira ◽

Cristiane Contigli ◽

José Roberto Lambertucci ◽

José Carlos Serufo ◽

Virmondes Rodrigues

Keyword(s):

Tumor Necrosis Factor ◽

Tumor Necrosis ◽

Ultrasound Examination ◽

Enzyme Linked Immunosorbent Assay ◽

Serum Samples ◽

Necrosis Factor Alpha ◽

Tnf Α ◽

Cytokine Levels ◽

Factor Alpha ◽

Necrosis Factor

ABSTRACT Cytokine levels were compared between schistosomiasis patients affected by intense fibrosis defined by ultrasound examination and graded from F-0 to F-3. The concentrations of interleukin-1β (IL-1β), IL-4, IL-5, IL-10, IL-13, gamma interferon, and tumor necrosis factor alpha (TNF-α) were determined by enzyme-linked immunosorbent assay of serum samples. Levels of IL-4, IL-5, and TNF-α in the sera of F-3 patients were significantly higher than those found in F-0 individuals, while levels of IL-13 were lower. Levels of IL-4, IL-5, and TNF-α in serum were significantly higher in F-3 males than in F-0 males or F-3 females. Conversely, levels of IL-13 were significantly lower in F-3 females than in F-0 females and males.

Download Full-text

The Relationship of Hemoglobin, Interleukin-10 and Tumor Necrosis Factor Alpha Levels In Asymptomatic Malaria Patients in Trenggalek, Jawa Timur, Indonesia

Molecular and Cellular Biomedical Sciences ◽

10.21705/mcbs.v3i1.37 ◽

2019 ◽

Vol 3 (1) ◽

pp. 13

Author(s):

Arif Rahman Nurdianto ◽

Heny Arwati ◽

Yoes Prijatna Dachlan ◽

Dyah Ayu Febiyanti

Keyword(s):

Tumor Necrosis Factor ◽

Tumor Necrosis ◽

Enzyme Linked Immunosorbent Assay ◽

High Morbidity ◽

Normal Person ◽

Asymptomatic Malaria ◽

Necrosis Factor Alpha ◽

Positive Correlation ◽

Tnf Α ◽

Necrosis Factor

Background: Malaria is still a universal health problem, especially in tropical countries because of high morbidity and mortality rates. Infection by Plasmodium falciparum and Plasmodium vivax could result in asymptomatic disease of malaria and be found in Trenggalek, Jawa Timur. Differences in pathogenesis among affected individuals are affected by many factors, and the immune system is one of them. Among substances involved in the malarial immunity is Tumor Necrosis Factor (TNF)-α and Interleukin (IL)-10, produced by the body's defense system as the reaction to the parasite. Therefore a study was designed to detect the level of TNF-α and IL-10 in asymptomatic malaria patients.Materials and Methods: A cross-sectional study was conducted. Thirty male asymptomatic malaria subjects, age 21 to 60 years were selected. Blood from each subject was collected and the levels of TNF-α and IL-10 were analyzed using enzyme-linked immunosorbent assay (ELISA) method. Significant values considered at p<0.05.Results: There was an increased level of TNF-α with the average of 218.760 pg/µL, and an increased level of IL-10 with an average of 257.574 pg/µL in asymptomatic malaria subjects. In normal person IL-10 level is 12.6 (8.5-16.7) pg/mL and the levels of TNF-α in normal person is 0-1.5 pg/mL because they are not produce. There was a positive correlation of TNF-α with IL-10 (r=0.332; p>0.05), and positive correlation between TNF-α and the rate of hemoglobin (r=0.002; p>0.05). IL-10 was correlated negatively with the rate of hemoglobin (r=-0.363; p<0.05).Conclusion: The results from this study conclude that TNF-α and IL-10 levels increase in asymptomatic malaria subjects.Keywords: asymptomatic malaria, TNF-α, IL-10, parasite, hemoglobin

Download Full-text

Induction of matrix metalloproteinase-1 by tumor necrosis factor-α is mediated by interleukin-6 in cultured fibroblasts of keratoconus

Experimental Biology and Medicine ◽

10.1177/1535370216650940 ◽

2016 ◽

Vol 241 (18) ◽

pp. 2033-2041 ◽

Cited By ~ 7

Author(s):

Genlai Du ◽

Chengxing Liu ◽

Xiaona Li ◽

Weiyi Chen ◽

Rui He ◽

...

Keyword(s):

Tumor Necrosis Factor ◽

Matrix Metalloproteinase ◽

Interleukin 6 ◽

Tumor Necrosis ◽

Tumor Necrosis Factor Α ◽

Enzyme Linked Immunosorbent Assay ◽

Tnf Α ◽

Factor Α ◽

Inflammatory Molecules ◽

Necrosis Factor

Inflammatory molecules and matrix metalloproteinase (MMPs) have been found over-expressed in the tear film of patients with keratoconus. However, the mechanistic link between inflammatory molecules and MMPs in the pathogenesis of keratoconus remains still elusive. Therefore, we investigated the effect of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) on MMP-1 expression and used IL-6 antibody (IL-6 Ab) to examine the role of IL-6 on TNF-α mediated regulation of MMP-1 in fibroblasts of normal cornea and keratoconus. Real-time polymerase chain reaction, Enzyme-linked immunosorbent assay, and Western blot data demonstrated that MMP-1 and IL-6 were expressed in fibroblasts of normal cornea and keratoconus. Levels of MMP-1 and IL-6 were significantly higher in keratoconus than normal cornea. TNF-α treatment led to a significant increase in IL-6 levels. IL-6 treatment induced MMP-1 synthesis in normal cornea and keratoconus. TNF-α increased MMP-1 expression in a dose- and time-dependent manner and this response was completely inhibited by the IL-6 Ab. In conclusion, these results indicate that fibroblasts of keratoconus shows increased levels of IL-6 and MMP-1 gene and protein expression and IL-6 mediates the TNF-α-induced MMP-1 expression.

Download Full-text

Assessment of tumor necrosis factor- alpha in gingivitis and periodontitis patients

International Journal of Dental Research ◽

10.14419/ijdr.v5i2.7874 ◽

2017 ◽

Vol 5 (2) ◽

pp. 108 ◽

Cited By ~ 1

Author(s):

Sajeev Shrestha ◽

Manisha Neupane ◽

Shivalal Sharma ◽

Madhab Lamsal

Keyword(s):

Tumor Necrosis Factor ◽

Tumor Necrosis Factor Alpha ◽

Tumor Necrosis ◽

Enzyme Linked Immunosorbent Assay ◽

Pocket Depth ◽

Necrosis Factor Alpha ◽

Cross Sectional ◽

Tnf Α ◽

Factor Alpha ◽

Necrosis Factor

Background: Tumor necrosis factor-α, one of the cytokines, is released in various chronic inflammatory diseases including periodontitis.Aims: To estimate the level of Tumor necrosis factor- alpha (TNF-α) in gingivitis and periodontitis patientsSettings and Design: A cross-sectional study was conducted. A total of 75 patients were recruited by purposive sampling technique among the patients visiting the Department of Periodontology and Oral Implantology during the period one year from August 2014 to July 2015.Material and methods: The recruited samples were divided into gingivitis and periodontitis groups based on clinical attachment level (CAL). A periodontitis subject was defined as having at least 4 sites with pocket depth (PD) >3mm and at least 4 sites with CAL>3 mm, while gingivitis group included the subjects having no CAL measurements greater than 3 mm with signs of inflammation. The TNF-α level was measured using the RayBio Human TNF-alpha ELISA (Enzyme-linked Immunosorbent Assay) kit.Results: The Median TNF-α interquartile range (IQR) (minimum-maximum) in gingivitis and periodontitis was 58.37 (32.63 – 198.77) and 111.89 (39.27 – 215.0) pg/ml, respectively.Conclusion: The level of TNF-α was found to be higher in periodontitis group compared to gingivitis group, although the result was not statistically significant.

Download Full-text

Enhancement of Neonatal Innate Defense: Effects of Adding an N-Terminal Recombinant Fragment of Bactericidal/Permeability-Increasing Protein on Growth and Tumor Necrosis Factor-Inducing Activity of Gram-Negative Bacteria Tested in Neonatal Cord Blood Ex Vivo

Infection and Immunity ◽

10.1128/iai.68.9.5120-5125.2000 ◽

2000 ◽

Vol 68 (9) ◽

pp. 5120-5125 ◽

Cited By ~ 37

Author(s):

Ofer Levy ◽

Richard B. Sisson ◽

Jonathan Kenyon ◽

Eric Eichenwald ◽

Ann B. Macone ◽

...

Keyword(s):

Tumor Necrosis Factor ◽

Klebsiella Pneumoniae ◽

Cord Blood ◽

Tumor Necrosis ◽

Enzyme Linked Immunosorbent Assay ◽

Gram Negative Bacteria ◽

Gram Negative ◽

Innate Defense ◽

Tnf Α ◽

Necrosis Factor

ABSTRACT Innate defense against microbial infection requires the action of neutrophils, which have cytoplasmic granules replete with antibiotic proteins and peptides. Bactericidal/permeability-increasing protein (BPI) is found in the primary granules of adult neutrophils, has a high affinity for lipopolysaccharides (or “endotoxins”), and exerts selective cytotoxic, antiendotoxic, and opsonic activity against gram-negative bacteria. We have previously reported that neutrophils derived from newborn cord blood are deficient in BPI (O. Levy et al., Pediatrics 104:1327–1333, 1999). The relative deficiency in BPI of newborns raised the possibility that supplementing the levels of BPI in plasma might enhance newborn antibacterial defense. Here we determined the effects of addition of recombinant 21-kDa N-terminal BPI fragment (rBPI21) on the growth and tumor necrosis factor (TNF)-inducing activity of representative gram-negative clinical isolates. Bacteria were tested in citrated newborn cord blood or adult peripheral blood. Bacterial viability was assessed by plating assay, and TNF-α release was measured by enzyme-linked immunosorbent assay. Whereas adult blood limited the growth of all isolates exceptKlebsiella pneumoniae, cord blood also allowed logarithmic growth of Escherichia coli K1/r and Citrobacter koseri. Bacteria varied in their susceptibility to rBPI21's bactericidal action: E. coliK1/r was relatively susceptible (50% inhibitory concentration [IC50], ∼10 nM), C. koseri was intermediate (IC50, ∼1,000 nM), Klebsiella pneumoniae was resistant (IC50, ∼10,000 nM), andEnterobacter cloacae and Serratia marcescens were highly resistant (IC50, >10,000 nM). All isolates were potent inducers of TNF-α activity in both adult and newborn cord blood. In contrast to its variable antibacterial activity, rBPI21 consistently inhibited the TNF-inducing activity of all strains tested (IC50, 1 to 1,000 nM). The antibacterial effects of rBPI21 were additive with those of a combination of conventional antibiotics typically used to treat bacteremic newborns (ampicillin and gentamicin). Whereas ampicillin and gentamicin demonstrated little inhibition of bacterially induced TNF release, addition of rBPI21 either alone or together with ampicillin and gentamicin profoundly inhibited release of this cytokine. Thus, supplementing newborn cord blood with rBPI21 potently inhibited the TNF-inducing activity of a variety of gram-negative bacterial clinical pathogens and, in some cases, enhanced bactericidal activity. These results suggest that administration of rBPI21 may be of clinical benefit to neonates suffering from gram-negative bacterial infection and/or endotoxemia.

Download Full-text

Effects of Eight-Week Combined Resistance and Endurance Training on Salivary Interleukin-12, Tumor Necrosis Factor, Cortisol, and Testosterone Levels in Patients with Breast Cancer

International Journal of Cancer Management ◽

10.5812/ijcm.109039 ◽

2021 ◽

Vol 14 (2) ◽

Author(s):

Amirabbas Monazzami ◽

Roya Momenpur ◽

Elham Alipour ◽

Kheirollah Yari ◽

Mehrdad Payandeh

Keyword(s):

Breast Cancer ◽

Tumor Necrosis Factor ◽

Endurance Training ◽

Tumor Necrosis ◽

Training Group ◽

Interleukin 12 ◽

Enzyme Linked Immunosorbent Assay ◽

Testosterone Levels ◽

Tnf Α ◽

Necrosis Factor

Background: In recent years, several studies have shown the association between exercise and decreased risk of mortality in patients with breast cancer. However, the effects of combined resistance and endurance training on salivary Interleukin-12 (IL-12), tumor necrosis factor (TNF-α), Cortisol, and Testosterone levels in patients with breast cancer have not been investigated. Objectives: This study aimed at determining the effect of 8 weeks of combined resistance and endurance training on salivary IL-12, TNF-α, Cortisol, and Testosterone levels in women with breast cancer. Methods: Forty-two postmenopausal women with breast cancer were randomly selected and divided into training (intervention) and control groups. The training group performed resistance training with 2 to 3 sets, 10 to 18 repetitions, 50 to 70% 1 repetition maximum (1RM), and aerobic exercise with 50 to 70% maximum heart rate (maxHR) (12-14 degrees borg scale) for 20 to 40 minutes for 8 weeks. The salivary IL-12, TNF-α, cortisol, and testosterone levels were measured, using the enzyme-linked immunosorbent assay (ELISA) method. Two-way analysis of variance repeated measure was also used to analyze variance with the confidence interval of 95%. Results: In the training group, there was a significant decrease in salivary TNF-α levels, cortisol, TNF-α/IL-12 ratio, and variables of weight, fat percentage, body mass index (BMI), and waist circumference (P < 0.05). Also, the results showed a significant increase in salivary testosterone and testosterone/cortisol ratio in the intervention group (P < 0.05). However, no significant changes were observed in the interaction between-group and time in IL-12 and waist–hip ratio (WHR) values (P > 0.05). Conclusions: The results indicate that resistance and endurance training could be used as a useful method to improve salivary pro-inflammatory factors and hormonal levels in patients with breast cancer. Medical oncologists can underline a resistance and endurance training program for patients with breast cancer under their care.

Download Full-text